The proportion of embryos displaying a delay in furrow formation drops from 25% observed for embryos derived from Fmr13/Df(3R)Exel6265 females to below 25% for embryos from RpS131/+;Fmr13/Df(3R)Exel6265 mothers.
Homozygous mutant RpI135k16513 clone survival can be rescued by genetically reducing the growth of surrounding cells by making them heterozygous for a dominant Minute RpS131 mutant. In this case, RpI135k16513 mutant clones are recovered in all RpS131 heterozygous discs that are examined at 96hrs after clone induction, a time-point at which all clones are normally eliminated in a wild-type background. However, these clones colonise a significantly smaller area of RpS131/+ discs compared with wild-type clones (28.8% compared to 67.8% of the total disc area), indicating that they are still growth impaired. Compared to surrounding heterozygote RpS131 or wild-type cells, RpI135k16513 mutant clone cells exhibit no significant change in either cell size or cell-cycle phasing.