FlyBase Allele Report: Dmel\Taf1[XS-2232]

General Information

Symbol

Dmel\Taf1^XS-2232

Species

D. melanogaster

Name

FlyBase ID

FBal0048987

Feature type

allele

Associated gene

Dmel\Taf1

Associated Insertion(s)

Carried in Construct

Also Known As

SR3-5^XS-2232

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Karim et al., 1996)

Progenitor genotype

Cytology

Description

Amino acid replacement: V1072D.

(Wassarman et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:6,650,942..6,650,942 [+]

Nucleotide change:

T6650942A

Amino acid change:

Reported amino acid change:

V1072D

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement (Figure 4: RWEVIDV changes to RWEDIDV).

(Wassarman et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Wassarman et al., 2000)

lethal | recessive

(Karim et al., 1996)

partially lethal - majority die

(Wassarman et al., 2000)

Phenotype Manifest In

Detailed Description

Statement

Reference

Taf1^EP421/Taf1^XS-2232 flies have reduced bristle numbers on both male and female heads, are female sterile and are semi-lethal. They also display defects in oogenesis, ocelli development, and developmental timing. Ommatidia in Taf1^EP421/Taf1^XS-2232 flies are irregularly shaped and are not aligned in straight rows. The number of interommatidial bristles are reduced, multiple bristles sometimes originate from a single site and bristles are randomly distributed. The number of photoreceptor cells per ommatidium is also altered in these flies. Approximately 10% of ommatidia are missing one outer photoreceptor, and 14% have an extra outer photoreceptor. Taf1^EP421/Taf1^XS-2232 flies lack ocelli. Bristles on the thorax show normal morphology but are shifted from the normal orderly pattern. About 5% of Taf1^EP421/Taf1^XS-2232 males show an abnormal rotation of their terminalia during pupation. Wing development in Taf1^EP421/Taf1^XS-2232 is also disrupted. The wing vein L4 bifurcates along the wing margin forming a delta. The shape and size of the wings appear normal as does the morphology of the other wing veins. Wings are often held out to the sides of the body rather than crossed over the body as seen in wild-type. Taf1^EP421/Taf1^XS-2232 females are sterile with very small ovaries. Taf1^EP421/Taf1^XS-2232 flies develop about 2 days more slowly than wild-type.

(Wassarman et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Taf1^XS-2232 is a suppressor of lethal phenotype of Ras85D^V12.sev, sev^S11.Tag:MYC

(Maixner et al., 1998)

Other

Statement

Reference

Ras85D^V12.sev, Taf1^XS-2232, sev^S11.Tag:MYC has viable phenotype

(Maixner et al., 1998)

Phenotype Manifest In

Enhancer of

Statement

Reference

Taf1^XS-2232 is an enhancer of embryonic epidermis phenotype of dl^unspecified

(Pham and Sauer, 2000)

Taf1^XS-2232 is an enhancer of embryonic/first instar larval cuticle phenotype of dl^unspecified

(Pham and Sauer, 2000)

NOT Enhancer of

Statement

Reference

Taf1[+]/Taf1^XS-2232 is a non-enhancer of wing vein | ectopic phenotype of Snr1^E1

(Marenda et al., 2004)

Suppressor of

Statement

Reference

Taf1^XS-2232 is a suppressor of phenotype of Ras85D^V12.sev

(Karim et al., 1996)

NOT Suppressor of

Statement

Reference

Taf1[+]/Taf1^XS-2232 is a non-suppressor of wing vein | ectopic phenotype of Snr1^E1

(Marenda et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

When Taf1^XS-2232 is added to dl^unspecified/+ sensitized embryos they exhibit a dorsalised and twisted body pattern.

(Pham and Sauer, 2000)

The lethality caused by sev^{S11.T:Hsap\MYC} in combination with Ras85D^V12.sev is suppressed by Taf1^XS-2232.

(Maixner et al., 1998)

Suppresses the rough eye phenotype of Ras85D^V12.sev. Slightly suppresses the phenotype of Ras85D^N17.sev. Has little or no effect on the phenotype of phl::tor^13D.hs.sev. Suppresses the phenotype of Ras85D::Src64B^{V12.ΔCAAX.sev}. Has little or no effect on the lethality of phl¹². Has little or no effect on the phl¹² eye phenotype. Has little or no effect on the Dsor1^XS520 eye phenotype. Has little or no effect on the aop^S2382 eye phenotype.