Allele Ctet\TeTxLC^{IMPTNT-V.Scer\UAS}

General Information
Symbol	Ctet\TeTxLC^{IMPTNT-V.Scer\UAS}	Species	C. tetani
Name		FlyBase ID	FBal0049204
Feature type	allele	Associated gene	Ctet\TeTxLC

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Sweeney et al., 1995, de Celis, 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Mutated Ctet\TeTxLC gene fragment (amino acid replacement: H233V and H237V) is expressed under the control of the Scer\UAS Scer\GAL4 binding sites. (Sweeney et al., 1995)
Carried in construct	P{UAS-TeTxLC.(-)V} (Mee et al., 2004, Fishilevich and Vosshall, 2005, Friggi-Grelin et al., 2003, Rodriguez Moncalvo and Campos, 2005, Renn et al., 1999, Suster and Bate, 2002, Luan et al., 2006, Inoshita and Tanimura, 2006, Sweeney et al., 1995, Mathew et al., 2003, Baines et al., 2002, Blanchardon et al., 2001, Gomez-Diaz et al., 2004, Baines et al., 2001, Kaneko et al., 2000, Baines et al., 1999, Allen et al., 1999, Hiesinger et al., 1999, Olofsson and Page, 2005, Tsai et al., 2004, de Celis et al., 1996, Bray and Amrein, 2003, Tracey et al., 2003, de Celis et al., 1997, de Celis, 1997, Suster et al., 2003, Devaud et al., 2003, Johard et al., 2009, Lu et al., 2012, Kwon et al., 2010, Morton et al., 2008, Ejima et al., 2007, Roy et al., 2007, Ai et al., 2010, Sadaf et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	viable, with Scer\GAL4^Gyc-89Da.1.9 (Morton et al., 2008) viable, with Scer\GAL4^Gyc-89Db.3.4 (Morton et al., 2008) wild-type, with Scer\GAL4^eve.RRC (Baines et al., 1999)
Phenotype Manifest In
	dorsal medial muscle, with Scer\GAL4^Trh.PB (Sadaf et al., 2012)
Detailed Description
	Statement Reference Flies expressing Ctet\TeTxLC[IMPTNT-V.Scer\UAS] under the control of Scer\GAL4[Trh.PB] show normal flight ability in a cylinder drop test. Air-puff stimulated flight responses of the dorsal longitudinal indirect flight muscle (DLM) of tethered flies expressing Ctet\TeTxLC[IMPTNT-V.Scer\UAS] under the control of Scer\GAL4[Trh.PB] are normal. (Sadaf et al., 2012) Males expressing Ctet\TeTxLC[IMPTNT-V.Scer\UAS] under the control of either Scer\GAL4[Or65a.4.7] or Scer\GAL4[Or65a.PF] and treated with cis-vaccenyl acetate (cVa) show an inhibition of initial courtship of mature virgin females, as do wild-type males treated with cVa. (Ejima et al., 2007) The normal architecture of the CSD interneuron (CSDn) is only slightly altered by targeted expression of Ctet\TeTxLC[IMPTNT-V.Scer\UAS] driven by Scer\GAL4[αTub84B.PP]. (Roy et al., 2007) Ctet\TeTxLC^{IMPTNT.Scer\UAS}; Scer\GAL4^NP1017 adults starved of water for 2 hours a normal proboscis extension response when their prothoracic legs are exposed to water. (Inoshita and Tanimura, 2006) Expression of Ctet\TeTxLC^{IMPTNT-V.Scer\UAS} throughout the CNS, using Scer\GAL4^elav-C155 as the driver, does not affect embryonic ventral cord condensation. (Olofsson and Page, 2005) Expression of Ctet\TeTxLC^{IMPTNT-V.Scer\UAS} under the control of Scer\GAL4^GMR.PF does not affect 5-HT arborization in larval brains. (Rodriguez Moncalvo and Campos, 2005) When Ctet\TeTxLC^{IMPTNT-V.Scer\UAS} is driven by Scer\GAL4^P2.4.Pdf no effect is seen on sensitivity to cocaine. (Tsai et al., 2004) The olfactory attraction and repulsion behaviour of flies expressing Ctet\TeTxLC[IMPTNT-V.Scer\UAS] under the control of either Scer\GAL4[85b] or Scer\GAL4[oeC] in response to various chemicals, including butanol, benzaldehyde, propionic acid and acetone, does not significantly differ from that of wild-type. (Devaud et al., 2003) Adults expressing Ctet\TeTxLC^TNT.Scer\UAS under the control of Scer\GAL4^ple.PF show a normal response in an assay in which flies are placed in a column with a conic bottom end and then suddenly tapped to the bottom to startle them. (Friggi-Grelin et al., 2003) When Ctet\TeTxLC^{IMPTNT-V.Scer\UAS} is driven by Scer\GAL4^109(2)80, mutant larvae exhibit a normal noxious heat response. (Tracey et al., 2003) The average locomotor activity plots in light:dark (LD) cycles of flies expressing Ctet\TeTxLC^{IMPTNT-V.Scer\UAS} under the control of Scer\GAL4^per.PK or Scer\GAL4^tim.PE is indistinguishable from those of control flies. Expression of Ctet\TeTxLC^{IMPTNT-V.Scer\UAS} under the control of Scer\GAL4^per.PK under constant darkness conditions does not result in a decrease in rhythmicity of locomotor behaviour. Expression of Ctet\TeTxLC^{IMPTNT-V.Scer\UAS} under the control of Scer\GAL4^tim.PE under constant darkness conditions results in a decrease in in the number of rhythmic flies in a locomotor activity assay. (Kaneko et al., 2000) When expression is driven by Scer\GAL4^c17 in the giant fibre the response in the TTM to repetitive firing is wild type. (Allen et al., 1999) When Ctet\TeTxLC^{IMPTNT-V.Scer\UAS} is driven by Scer\GAL4^eve.RRC in aCC neurons, excitatory post-synaptic currents (epscs) are present in 14/18 neurons. Sustained inward currents are also seen in 14/18 neurons. (Baines et al., 1999) Flies expressing Ctet\TeTxLC^{IMPTNT-V.Scer\UAS} under the control of Scer\GAL4^MZ1369 have no obvious developmental defects. The wild-type columnar organisation of the medulla is recognisable in the optic lobes and the arborisations of the Fas2-positive lamina monopolar cells in the distal medulla are normal. (Hiesinger et al., 1999) Scer\GAL4 mediated expression has no effect on viability. (Sweeney et al., 1995)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference per[01] mutant files co-expressing per[Scer\UAS.cBa] and Ctet\TeTxLC[TNT.Scer\UAS] under the control of Scer\GAL4[Mai179] display morning anticipation in their locomotor activity, but they completely lose evening anticipation, compared with controls with Ctet\TeTxLC[IMPTNT-V.Scer\UAS] expression. (Johard et al., 2009)
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	28840 w^; P{UAS-TeTxLC.(-)V}A2 28841 w^; P{UAS-TeTxLC.(-)V}B3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Ctet\TeTxLC^{IMPTNT-V.Scer\UAS} TeTxLC^IMPTNT-V.UAS
Name Synonym
Secondary FlyBase IDs

References ( 35 )

Generate a list of	All references relating to this allele ( 35 )

List References by type	Research paper ( 35 )
Recent research papers ( 2 )
	Lu et al., 2012, PLoS Genet. 8(3): e1002587 ppk23-Dependent Chemosensory Functions Contribute to Courtship Behavior in Drosophila melanogaster. [FBrf0217844] Sadaf et al., 2012, PLoS ONE 7(9): e46405 Synaptic Activity in Serotonergic Neurons Is Required for Air-Puff Stimulated Flight in Drosophila melanogaster. [FBrf0219558] Show all research papers ...

Allele Ctet\TeTxLCIMPTNT-V.Scer\UAS

Allele Ctet\TeTxLC^{IMPTNT-V.Scer\UAS}