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General Information
Symbol
Dmel\Tm1eg9
Species
D. melanogaster
Name
FlyBase ID
FBal0049223
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
TmIIeg9
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of P{lacW}Tm1gs1, resulting in a greatly reduced expression of Tm1 isoforms C, H and I in homozygous ovaries.

Excision of the P{lacW} element.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Tm1eg9/Tm1eg1 mutant oocytes show impaired plus-end-directed motility of osk messenger ribonucleoprotein particles as they fail to show the dominance of plus-end-directed runs over the minus-end-directed runs normally observed in wild-type oocytes) and delocalization of the osk mRNA (also observed in Tm1eg9 homozygotes), the females display the grandchildless phenotype.

96.3% of the progeny of homozygous females are germ cell-less.

Strong germ cell-less phenotype.

Embryos fail to form pole cells. Transplantation of wild type pole cells into homozygous embryos results in wild type egg chambers and wild type embryos.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

Tm1[+]/Tm1eg9 is an enhancer of visible phenotype of br1

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Tm1eg20/Tm1eg9 has germline cell phenotype, enhanceable by E(To)1010/E(To)10[+]

Tm1eg20/Tm1eg9 has germline cell phenotype, enhanceable by E(To)4[+]/E(To)44

Tm1eg20/Tm1eg9 has germline cell phenotype, enhanceable by E(To)66/E(To)6[+]

Tm1eg20/Tm1eg9 has germline cell phenotype, enhanceable by Rab11ETo11/Rab11[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)1010/E(To)10[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)1212/E(To)12[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)13[+]/E(To)1313

Tm1eg20/Tm1eg9 has germline cell phenotype, enhanceable by osk[+]/oskETo7

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)11/E(To)1[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)4[+]/E(To)44

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)55/E(To)5[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)66/E(To)6[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)8[+]/E(To)88

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)9[+]/E(To)99

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by Rab11ETo11/Rab11[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by Rab11[+]/Rab11ETo3

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by magoETo2/mago[+]

Tm1eg20/Tm1eg9 has germline cell phenotype, enhanceable by vasETo14/vas[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by vasETo14/vas[+]

Tm1eg20/Tm1eg9 has germline cell phenotype, enhanceable by E(To)11/E(To)1[+]

Suppressed by
Statement
Reference

Tm1eg20/Tm1eg9 has germline cell phenotype, suppressible by E(To)1212/E(To)12[+]

Tm1eg20/Tm1eg9 has germline cell phenotype, suppressible by E(To)13[+]/E(To)1313

Enhancer of
Statement
Reference

Tm1[+]/Tm1eg9 is an enhancer of leg phenotype of br1

Other
Additional Comments
Genetic Interactions
Statement
Reference

Expression of Khc401.Scer\UAS.P\T.T:MS2\MCP under the control of Scer\GAL4osk.T:Hsim\VP16 rescues the impaired plus-end-directed motility of osk messenger ribonucleoprotein particles (restoring the dominance of plus-end-directed runs over the minus-end-directed runs) and the posterior pole localization of osk mRNA in the mutant oocytes.

Dominantly weakly enhances the frequency of br1 mutant animals with malformed legs.

66% of eggs derived from Tm1eg9 +/Tm1el4 osk1 females do not develop into viable larvae. In 44% of the dead eggs, embryogenesis does not commence, or is terminated before cuticle formation, while 56% of the dead eggs have a characteristic "posterior group" phenotype with missing abdominal segments.

Second site non-complementing phenotype with zipEbr but not zipmhc-c6.1.

Xenogenetic Interactions
Statement
Reference

The expression of Mmus\SynpoScer\UAS.cWa via Scer\GAL4nos.UTR.T:Hsim\VP16 suppresses the grandchildless phenotype of Tm1eg9 mutants.

Complementation and Rescue Data
Comments

Expression of Tm1Scer\UAS.P\T.I.T:Avic\GFP-EMD under the control of Scer\GAL4osk.T:Hsim\VP16 rescues the grandchildless phenotype of Tm1eg9/Tm1eg1 and restores the posterior pole localization of osk mRNA in the mutant oocytes.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (7)