FlyBase Allele Report: Dmel\aly[5]

General Information

Symbol

Dmel\aly⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0049286

Feature type

allele

Associated gene

Dmel\aly

Associated Insertion(s)

H{}aly⁵

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Lin et al., 1996)

Progenitor genotype

Associated Insertion(s)

H{}aly⁵

Cytology

Description

Insertion of hobo into aly transcription unit, with a 10bp duplication of base pairs 1179-1188 (which lie within the open reading frame, region of amino acids 315-317).

(White-Cooper et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | recessive

(Lin et al., 1996)

male sterile | recessive

(Lin et al., 1996)

Phenotype Manifest In

nuclear chromosome & primary spermatocyte

(Jiang et al., 2007)

nuclear lamina | adult stage | male (with aly²)

(Trost et al., 2016)

spermatocyte

(Lin et al., 1996)

testis

(Lin et al., 1996)

Detailed Description

Statement

Reference

aly²/aly⁵ adult heterozygote males show morphological defects of the nuclear lamina in stage S4-S6 spermatocytes (the lamina has distinctly more ballon-like shape, lacking the deformations that correlate with chromosome distribution seen in wild-type), while in stage S3 the lamina is of wild-type appearance.

(Trost et al., 2016)

Nucleolar organisation is altered in aly⁵ mutant testes cells.

(Doggett et al., 2011)

Chromosomes in mutant primary spermatocytes are apposed to the nuclear envelope (as occurs in wild-type primary spermatocytes), but they are fuzzier and less well defined than in wild type.

(Jiang et al., 2007)

Early stages of spermatogenesis up through mature primary spermatocytes are present and normal. Postmeiotic stages are completely absent. Mutant testes have an abundance of mature primary spermatocytes with large, round nuclei and prominent nucleoli. Spermatocytes arrest in the G₂/M transition of meiosis I with partial chromosome condensation.

(Lin et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Morphologically, aly⁵ mutants expressing wuc^{dsRNA.Scer\UAS} under the control of Scer\GAL4^{bam.T:Hsim\VP16} are indistinguishable from single mutant testes. Nucleolar disorganisation, as found in aly⁵ mutants, is unaffected by expression of wuc^{dsRNA.Scer\UAS}.

(Doggett et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

aly^z3-1393

(White-Cooper et al., 2000)

aly^z3-3504

(White-Cooper et al., 2000)

aly^z3-4302

(White-Cooper et al., 2000)

aly^z3-4307

(White-Cooper et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

aly⁵

(Laktionov et al., 2018, Trost et al., 2016, Caporilli et al., 2013, Doggett et al., 2011, Di Cara et al., 2010, Jiang et al., 2007, Kalmykova et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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