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General Information
Symbol
Dmel\cortRH65
Species
D. melanogaster
Name
FlyBase ID
FBal0049393
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G6674959A

Reported nucleotide change:

G1378A

Amino acid change:

W431term | cort-PA; W431term | cort-PB

Reported amino acid change:

W431term

Comment:

TGG to TGA

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: W431term.

Nucleotide substitution: G1378A.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

0-2 hours eggs derived from cortQW55/cortRH65 females contain two spindles near the anterior cortex of the egg. Both of the spindles contain a single X-chromosome signal, indicating arrest in metaphase II.

A small percentage of embryos laid by grauRM61/Df(2R)Pu-D17 or grauQF31/Df(2R)Pu-D17 females develop into adult flies.

Embryos derived from homozygous females do not develop.

cort embryos do not develop to the cellular blastoderm stage. Embryos exhibit a complete absence of microtubule containing spindles, embryos do not initiate mitotic divisions. Oocyte meiosis is initiated but is arrested before completion. Sperm tails are present in embryos indicating a defect in oocyte fertilisation.

Females lay eggs that arrest in meiosis II, the earliest defect is in anaphase I. Premature sister chromatid separation does not rescue the meiotic defects, as shown in mei-S3321 double mutants. Male meiosis and mitosis are normal.

Eggs derived from homozygous females initiate development and cytoplasmic clearing occurs in a narrow zone around the egg periphery (in wild-type embryos this process is coupled to the arrival of the nuclei at the periphery). The eggs do not seem to develop beyond this stage; pole cells are not formed and cellularisation does not occur. However, about two hours after cytoplasmic clearing, the egg periphery starts to show local contractions, in what might be an attempt at gastrulation. These contractions eventually lead to eggs which typically have one or two condensed yolk balls surrounded by more transparent cytoplasm.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

cortRH65/cort[+] is an enhancer of lethal | maternal effect phenotype of grauRM61/Df(2R)Pu-D17

cortRH65/cort[+] is an enhancer of lethal | maternal effect phenotype of Df(2R)Pu-D17/grauQF31

Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

74% of eggs derived from fzy6/fzy7 ; cortQW55/cortRH65 females raised at 29oC contain two spindles, each with a single X-chromosome signal, indicating arrest in metaphase of meiosis II. The remaining 26% of eggs contain only a single spindle containing two X-chromosome signals, indicating an arrest in meiosis I.

The small percentage of developing embryos laid by grauRM61/Df(2R)Pu-D17 or grauQF31/Df(2R)Pu-D17 females is abolished if the females are also heterozygous for cortQW55.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
  • FBal0001770
References (13)