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General Information
Symbol
Dmel\dock13421
Species
D. melanogaster
Name
FlyBase ID
FBal0049421
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dockP2
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
transposable element insertion site
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{PZ} insertion 37bp downstream of the first exon-intron boundary.

Insertion components
P{PZ}dock13421
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

dock13421/+ mutant third instar larval eye discs do not show disrupted axon targeting from photoreceptor neurons to the brain optic lobes.

When mutant clones are made in the developing eye, no R-cell precursor nuclear migration phenotype was seen.

Photoreceptor cell axons fail to target the optic lobe properly in mutant larvae; crossing of axons, gaps in the lamina and blunt ended termination point in the medulla are seen.

The growth of retinula axons into the brain is disrupted in homozygous larvae, leading to a disruption of the lamina neuropile organisation. Homozygous embryos show a reduction in the longitudinal connectives and a slight fusion of the segmental commissures. The VUM neurons do not project normally.

In dock04723/dock13421 embryos, either the entire Bolwig's Nerve, or a subset of its axons project to ectopic positions. These defects have over a 90% penetrance.

In dock13421/dock3 mutant embryos longitudinal connectives are more wavy and more varied in thickness than wild type and the outermost one is occasionally interrupted. There are low penetrance defects in muscle organization. The muscle phenotype is not affected further in embryos lacking both maternal and zygotic dock activity. dock13421/dock3 mutant embryos (and homozygotes of both alleles) show a variable absence of the synapse between RP3 and muscles 7 and 6. Outgrowth of RP3 from the CNS is normal. Synapse formation eventually occurs, but is delayed. No ectopic synapses occur. This phenotype is identical to that of lbmY13. Maternal loss of dock expression enhances the CNS longitudinal axon defects, but not the RP3 synapse formation delay.

Some homozygotes survive to adulthood. These flies are sluggish and uncoordinated, dying within a few days after eclosion. Homozygous third instar larvae exhibit defects in receptor cell fasciculation, targeting and retinotopy. Receptor cell axons terminate at different depths in the developing lamina and form clumps of terminals instead of an even array. Bundles project from these regions into the medulla. Some bundles project along abnormal paths.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

dock13421 has photoreceptor cell & axon phenotype, enhanceable by Hem[+]/HemΔ2-6

NOT Enhanced by
Statement
Reference

dock13421 has phenotype, non-enhanceable by lbmY13

NOT suppressed by
Statement
Reference

dock13421 has phenotype, non-suppressible by lbmY13

Enhancer of
Statement
Reference

dock[+]/dock13421 is an enhancer of photoreceptor cell & axon phenotype of HemΔ2-6

dock13421 is an enhancer of eye photoreceptor cell & growth cone phenotype of msn03349

dock13421 is an enhancer of lamina phenotype of msn03349

NOT Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

dock13421/+; Dys8-2/+ double heterozygous mutant third instar larval eye discs do not show disrupted axon targeting from photoreceptor neurons to the brain optic lobes.

dock13421/+; Df(3R)Dl-X43/+ double heterozygous mutant third instar larval eye discs do not show disrupted axon targeting from photoreceptor neurons to the brain optic lobes.

Dg323/dock13421 double heterozygous mutant third instar larval eye discs show disrupted axon targeting from photoreceptor neurons to the brain optic lobes.

dock13421/+ ; InRex15/+ double heterozygotes show defects in photoreceptor axon projections in the medulla; gaps in the R7 terminal array and uninnervated areas are seen. These defects are seen in larvae and adults.

One copy of dock13421 enhances the axonal projection phenotype of HemΔ2-6 homozygous larvae. One copy of HemΔ2-6 enhances the axonal projection phenotype of dock13421 homozygous larvae.

The msn03349 R1-R6 photoreceptor cell phenotype is dominantly enhanced by dock13421; the R1-R6 termination site at the lamina becomes more disorganised and photoreceptor cell growth cones are much less expanded.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (9)