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General Information
Symbol
Dmel\dpn7
Species
D. melanogaster
Name
FlyBase ID
FBal0049433
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

A8231686C

Reported nucleotide change:

A404C

Amino acid change:

K45N | dpn-PA

Reported amino acid change:

K45N

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: K45N. K45N falls in the basic region of the bHLH region. Nucleotide substitution: A404C (nucleotide positions as in cDNA sequence of FBrf0056120).

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Type II neuroblasts are completely absent in the brains of late third larval instar dpn1/dpn7 larvae. The number of type I neuroblasts is close to that of wild type at 9-12 hours after larval hatching (ALH), but is reduced compared to wild type at 48 hours ALH, and does not reduce further from 48 hours to 96 hours ALH.

Mushroom body neuroblasts are progressively lost in dpn1/dpn7 mutants: at the late third instar larval stage nearly half of the mushroom body neuroblasts are missing, and by the start of pupariation, 90% of them are lost.

Homozygous mushroom body neuroblast clones generated immediately after larval hatching have mostly lost their neuroblasts by 1-2 days after pupariation, in contrast to wild-type control clones, which always contain a neuroblast at this stage.

Heterozygous larvae show a slight decrease in the number of type II neuroblasts in the brain compared to wild type.

The few Df(2R)dpn-2/dpn7 mutant escapers that are seen are uncoordinated and flightless. The total number of neuroblasts is reduced during the larval stages. Type II neuroblasts are never observed at late stages of larval development although one is occasionally seen in early larval brains.

dpn7 mutant clones generated in type II neuroblasts are smaller than control clones. In contrast, clones that originate in type I clones are similar in size to controls.

Strongly feminizes triploid intersexes. Degree of feminization of the foreleg is unusually high (35% of forelegs are entirely female). May be cell lethal in germ-line clones.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Suppressor of
Statement
Reference

dpn7/dpn[+] is a suppressor of lethal | female phenotype of sisA1

dpn7/dpn[+] is a suppressor of lethal | female phenotype of da1

dpn7/dpn[+] is a suppressor of lethal | female phenotype of scsisB-1

NOT Suppressor of
Phenotype Manifest In
NOT Enhanced by
Statement
Reference

dpn7 has type II neuroblast | larval stage phenotype, non-enhanceable by N+/N55e11

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

dpn7 has type II neuroblast | larval stage phenotype, non-suppressible by N+/N55e11

NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

prosV17/+ partially rescues the reduction in number of type I neuroblasts which is seen in the brains of late third larval instar dpn1/dpn7 larvae.

The decrease in the number of type II neuroblasts in the brain that is seen in dpn7/+ larvae is unaffected if the animals are also heterozygous for N55e11.

dpn7 does not suppress the ectopic neuroblast phenotype seen when clones expressing Nint.G.Scer\UAS are induced under the control of Scer\GAL4αTub84B.PL.

dpn-mediated suppression of sisA female lethality is entirely zygotic.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Shows complex complementation behavior. Complementation between mutant dpn alleles reveals differences in strength but little male-specific lethality.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)