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General Information
Symbol
Dmel\dshUAS.cAa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Axelrod
FlyBase ID
FBal0049443
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dsh, UAS.Dsh, UASdsh
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of dsh cDNA is governed by UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Overexpression of dshScer\UAS.cAa under the control of Scer\GAL4prd.RG1 in embryos produces clear expansions of the naked cuticle zones that are interspersed between the ventral denticle belts of wild-type embryos.

Expression of dshScer\UAS.cAa under the control of Scer\GAL4btl.PS results in dorsal trunks that are shorter than normal and are discontinuous.

Expression of dshScer\UAS.cAa in the retina behind the furrow using Scer\GAL4GMR.PF results in the projection of ventral R axons into the dorsal lamina.

Expression of dshScer\UAS.cAa under the control of Scer\GAL4hs.PB using four days of pulsed heat shock treatment consistently produces more follicle cells that accumulate between egg chambers. These extra follicle cells form long stalks with multiple rows of cells instead of one row in the wild type.

When expression is driven by Scer\GAL4ptc-559.1 at 18oC adults often eclose and a multiple wing hair phenotype results. In addition, ecoptic bristles are found.

Expression of dshScer\UAS.cAa under the control of Scer\GAL4dpp.blk1 inhibits furrow initiation in the eye disc.

dshScer\UAS.cAa when driven by Scer\GAL4GMR.PF produces small bristle-less eyes devoid of ommatidia.

Expression of dshScer\UAS.cAa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in loss of denticles and expansion of smooth cuticle.

Expression of dshScer\UAS.cAa under the control of Scer\GAL4da.G32 results in a phenotype identical to that produced by overexpression of wg.

Expression of dshScer\UAS.cAa driven by Scer\GAL4109(2)80 does not give rise to any detectable dendritic phenotypes.

When expression of dshScer\UAS.cAa is driven by Scer\GAL4sd-SG29.1, the whole of the anterior compartment of the wing disc is covered in evenly spaced SOPs (sensory organ precursors).

Clones in the notum expressing dshScer\UAS.cAa under the control of Scer\GAL4Ubx.PdC develop extra macrochaetae.

Embryos expressing dshScer\UAS.cAa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 have naked cuticle.

When driven by Scer\GAL4ptc-559.1 duplication of the wg-dependent scutellar bristles occurs. Scer\GAL4rho-rhl-mediated expression causes an increase in the density of macrochaetae on the notum.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
NOT suppressed by
Suppressor of
Statement
Reference

dshUAS.cAa/Scer\GAL469B is a suppressor | partially of embryonic leading edge cell & actin filament phenotype of hep1

dshUAS.cAa is a suppressor of wing phenotype of Scer\GAL4bbg-C96, mamN.UAS

Other
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of dshScer\UAS.cAa and MadScer\UAS.cNa under the control of Scer\GAL4sca-537.4 results in wings with no L1 or L3 sensilla, ectopic mechanosensory bristles on the dorsal margin and expansion of the L3 vein distally.

None of the embryos that are mutant for both maternal and zygotic arr2 and express Scer\GAL4prd.RG1>dshScer\UAS.cAa exhibit broad stripes of excess cuticle.

Scer\GAL4prd.RG1>dshScer\UAS.cAa-overexpressing embryos are readily identifiable amongst wgl-17 homozygotes by their four wide naked zones interspersed between their denticle bands.

Co-expression of nkdΔR1S.Scer\UAS.T:Avic\GFP-EGFP only weakly rescues the eye phenotype caused by expression of dshScer\UAS.cAa under the control of Scer\GAL4GMR.PF.

dshScer\UAS.cAa; Scer\GAL4da.G32 partially suppresses the transformation of naked ventral cuticle to denticles and the shortening of the cuticle seen in wgl-17 homozygous embryos. The dorsal side of these cuticles have some 'warts'. dshScer\UAS.cAa; Scer\GAL4da.G32 suppresses the loss of dorsal ventral polarity in the leading edge cells of stage 13 wgl-17 homozygous embryos, but only weakly rescues elongation of these cells.

The addition of dshScer\UAS.cAa partially restores areas of naked cuticle to AxnScer\UAS.cHa, Scer\GAL4arm.PS mutants. Also an abundance of small denticles are seen.

The dorsal hole phenotype, loss of leading edge actin cable, and loss of adhesion between the amnioserosa and dorsal epidermis of hep1 mutant embryos are all partially suppressed by Scer\GAL469B with dshScer\UAS.cAa.

The multiple hair cell phenotype of dshScer\UAS.cAa expressed from Scer\GAL4ptc-559.1 is additive with the multiple hair phenotypes of fy2 and inIH56.

nkdScer\UAS.T:Hsap\MYC strongly suppresses the dshScer\UAS.cAa, Scer\GAL4GMR.PF phenotype restoring numerous bristles and ommatidia.

The addition of fz2ECD.GPI.Scer\UAS.T:Hsap\MYC to dshScer\UAS.cAa, Scer\GAL4GMR.PF results in some eye necrosis, but has a negligible effect on eye phenotype caused by dshScer\UAS.cAa and Scer\GAL4GMR.PF alone.

nkdScer\UAS.cZa strongly suppresses the dshScer\UAS.cAa, Scer\GAL4GMR.PF phenotype restoring numerous bristles and ommatidia. The addition of fz2ECD.GPI.Scer\UAS.T:Hsap\MYC to dshScer\UAS.cAa, Scer\GAL4GMR.PF results in some eye necrosis, but has a negligible effect on eye phenotype caused by dshScer\UAS.cAa and Scer\GAL4GMR.PF alone.

Expression of dshScer\UAS.cAa under the control of Scer\GAL4arm.T:Hsim\VP16 in an arm3 background gives rise to a cuticle with many small denticles, but with the length of an arm3 single mutant.

Smooth cuticle is restored in alternate segments to arrunspecified embryos lacking maternal and zygotic arr function when dshScer\UAS.cAa is expressed in these embryos under the control of Scer\GAL4prd.RG1.

Strongly suppresses the wing nicking phenotype seen in mamN.Scer\UAS, Scer\GAL4C96 flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

dshScer\UAS.cAa; Scer\GAL4da.G32 rescues the cuticles of embryos from dsh3 germline clones to normal length and suppresses the hole anterodorsal cuticle hole.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Used to show that over-expression of dsh (hence an increase in wg signalling) in the posterior wing compartment inhibits wing margin N activity.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
dshScer\UAS.cAa
dshUAS.cAa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Axelrod
Secondary FlyBase IDs
    References (29)