wings have altered shape, but hair polarity is disrupted in only a small proximal region. Posterior ridges of fjd1
homozygous wings also have a more anterior-posterior orientation than wild-type.
Homozygous clones in the dorsal adult abdomen reverse hair polarity behind the clone.
Hemizygous stage 16 embryos have longer dorsal trunks than normal.
An equator can easily be discerned in fjd1
eyes. 98.5% of ommatidia are correctly constructed and rotated in these animals. Flies expressing fjScer\UAS.cZa
under the control of Scer\GAL4αTub84B.PL
in a fjd1
background have a normal overall pattern of polarisation in the eye; an equator can easily be discerned. 92.9% of ommatidia are correctly constructed and rotated in these animals. Expression of fjScer\UAS.cZa
under the control of Scer\GAL4bi-omb3-GAL4
in a fjd1
background has a profound effect on ommatidial polarity; near the dorsal pole, large-scale reversals of ommatidial polarity are seen.
Within the anterior compartments of the adult abdominal cuticle, somatic clones of fjd1
only exhibit planar polarity defects when they are located in approximately the front half of the compartment. Within affected fjd1
clones, the rows of hairs are jumbled and hair orientation is disturbed: most of those at the back of the clone are reversed, as are the wild-type hairs behind it. The further anterior the clone, the more disturbance within and the more reversal of hair polarity behind. Some somatic clones of fjd1
in the anterior (hair producing) territory of the posterior compartment form dishevelled and incorrectly polarized hairs; however, most appear normal. These clones do not have non-autonomous effects.
The normal regular pattern of wing hairs pointing towards the distal end of the wing is essentially unchanged in homozygous mutant individuals, occasional minor defects in wing hair polarity are identified. Homozygous mutant clones in the wing produce striking disruptions in the normal pattern of wing hair polarity. This effect wherever clones appeared in the wing. Occasionally loss of wing hairs is seen within the presumptive mutant area. Sometimes striking cell non-autonomous effects are seen on the proximal side of mutant patches. Clones smaller than about 5x5 cells never give non-autonomous phenotypes. All clones larger than about 5x5 cells produce non-autonomous phenotypes in a region distal to the posterior cross vein. Larger clones also produce strong non-autonomous phenotypes in a region between veins 3 and 4, and weaker phenotypes are observed in a large region in the centre of the wing posterior to vein 4. Clones sometimes contain veins that have either been duplicated or diverted, so that they remain in wild-type tissue immediately adjacent to the clone. In addition ectopic regions of wing vein material are found in many cones and occasional mutant clones are found in which veins fade after entering mutant tissue. In homozygous mutant adults flies, hair polarity defects and hair swirls are seen in the a2 region about 50% of the time. When homozygous mutant clones are produced in the abdomen, in a2 and the anterior of a3, both cell-autonomous and non-autonomous planar polarity defects are seen. Approximately three rows of non-autonomous hair inversions are seen at the posterior side of clone, and major disruptions of hair polarity are seen within the clone. No phenotypes are seen when clones are generated in the rest of the abdomen. Legs from fjd1
homozygotes are shortened and display the characteristic fusion of the second and third tarsi. Wings from fjd1
homozygotes are shortened - specifically the region between the anterior and the posterior crossvein is significantly reduced, whilst the proximal wing regions are unaffected.
flies, exhibit occasional ommatidia with inverted polarity relative to their neighbours. In homozygous fjd1
somatic clones in the eye produce strong effects on dorsoventral ommatidial patterning. Clones lying close to the equator in either hemisphere of the eye result in dorsoventral inversions of polarity in 1-3 rows straddling the boundary of the clone furthest from the equator (polar boundary). This is non-autonomous as ommatidia beyond the polar boundary of clones were inverted. The closer the clone is to the equator the stronger the phenotype is seen. The phenotype tended to be stronger at the posterior ends of clones. Clones that straddle the equator have inverted ommatidia at both polar edges, but the equator remains.
Legs are shorter than wild type with four instead of five tarsal segments. The first three but not the fourth or the fifth tarsal segments are affected. The wing is reduced in length. Eye surface is deformed, though this phenotype is only evident for stronger alleles and has variable penetrance and expressivity.