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General Information
Symbol
Dmel\fjd1
Species
D. melanogaster
Name
FlyBase ID
FBal0049500
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:
FBrf0086339 : Deletion of approximately 4kb extending into fj transcribed region and removing at least the first 100 amino acids including a putative signal sequence. FBrf0123257 : All but the last 57 amino acids of the coding region are deleted, with no remaining in frame methionines. Position of mutation on reference sequence inferred by FlyBase curator based on author statement.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
All but the last 57 amino acids of the coding region are deleted, with no remaining in frame methionines.
Deletion of approximately 4kb extending into fj transcribed region and removing at least the first 100 amino acids including a putative signal sequence.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
abdominal segment 2 & macrochaeta
abdominal segment 2 & microchaeta
trichome & tergite | cell non-autonomous | somatic clone
Detailed Description
Statement
Reference
Homozygous fjd1 wings have altered shape, but hair polarity is disrupted in only a small proximal region. Posterior ridges of fjd1 homozygous wings also have a more anterior-posterior orientation than wild-type.
Homozygous clones in the dorsal adult abdomen reverse hair polarity behind the clone.
Hemizygous stage 16 embryos have longer dorsal trunks than normal.
An equator can easily be discerned in fjd1/fjN7 eyes. 98.5% of ommatidia are correctly constructed and rotated in these animals. Flies expressing fjScer\UAS.cZa under the control of Scer\GAL4αTub84B.PL in a fjd1/fjN7 background have a normal overall pattern of polarisation in the eye; an equator can easily be discerned. 92.9% of ommatidia are correctly constructed and rotated in these animals. Expression of fjScer\UAS.cZa under the control of Scer\GAL4bi-omb3-GAL4 in a fjd1/fjN7 background has a profound effect on ommatidial polarity; near the dorsal pole, large-scale reversals of ommatidial polarity are seen.
Somatic clones lacking fjt14.T:Hsap\GALNT3 induced in a fjd1/fjd1; fjt14.T:Hsap\GALNT3/+ background non-autonomously cause inversion of ommatidia.
Within the anterior compartments of the adult abdominal cuticle, somatic clones of fjd1 only exhibit planar polarity defects when they are located in approximately the front half of the compartment. Within affected fjd1 clones, the rows of hairs are jumbled and hair orientation is disturbed: most of those at the back of the clone are reversed, as are the wild-type hairs behind it. The further anterior the clone, the more disturbance within and the more reversal of hair polarity behind. Some somatic clones of fjd1 in the anterior (hair producing) territory of the posterior compartment form dishevelled and incorrectly polarized hairs; however, most appear normal. These clones do not have non-autonomous effects.
The normal regular pattern of wing hairs pointing towards the distal end of the wing is essentially unchanged in homozygous mutant individuals, occasional minor defects in wing hair polarity are identified. Homozygous mutant clones in the wing produce striking disruptions in the normal pattern of wing hair polarity. This effect wherever clones appeared in the wing. Occasionally loss of wing hairs is seen within the presumptive mutant area. Sometimes striking cell non-autonomous effects are seen on the proximal side of mutant patches. Clones smaller than about 5x5 cells never give non-autonomous phenotypes. All clones larger than about 5x5 cells produce non-autonomous phenotypes in a region distal to the posterior cross vein. Larger clones also produce strong non-autonomous phenotypes in a region between veins 3 and 4, and weaker phenotypes are observed in a large region in the centre of the wing posterior to vein 4. Clones sometimes contain veins that have either been duplicated or diverted, so that they remain in wild-type tissue immediately adjacent to the clone. In addition ectopic regions of wing vein material are found in many cones and occasional mutant clones are found in which veins fade after entering mutant tissue. In homozygous mutant adults flies, hair polarity defects and hair swirls are seen in the a2 region about 50% of the time. When homozygous mutant clones are produced in the abdomen, in a2 and the anterior of a3, both cell-autonomous and non-autonomous planar polarity defects are seen. Approximately three rows of non-autonomous hair inversions are seen at the posterior side of clone, and major disruptions of hair polarity are seen within the clone. No phenotypes are seen when clones are generated in the rest of the abdomen. Legs from fjd1 homozygotes are shortened and display the characteristic fusion of the second and third tarsi. Wings from fjd1 homozygotes are shortened - specifically the region between the anterior and the posterior crossvein is significantly reduced, whilst the proximal wing regions are unaffected.
fjd1/Df(2R)Pcl11B flies, exhibit occasional ommatidia with inverted polarity relative to their neighbours. In homozygous fjd1 somatic clones in the eye produce strong effects on dorsoventral ommatidial patterning. Clones lying close to the equator in either hemisphere of the eye result in dorsoventral inversions of polarity in 1-3 rows straddling the boundary of the clone furthest from the equator (polar boundary). This is non-autonomous as ommatidia beyond the polar boundary of clones were inverted. The closer the clone is to the equator the stronger the phenotype is seen. The phenotype tended to be stronger at the posterior ends of clones. Clones that straddle the equator have inverted ommatidia at both polar edges, but the equator remains.
Legs are shorter than wild type with four instead of five tarsal segments. The first three but not the fourth or the fifth tarsal segments are affected. The wing is reduced in length. Eye surface is deformed, though this phenotype is only evident for stronger alleles and has variable penetrance and expressivity.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Enhancer of
NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
fjd1 has crossvein phenotype, enhanceable by ds1/ds[+]
fjd1 has wing phenotype, enhanceable by ds1/ds[+]
fjd1 has crossvein phenotype, enhanceable by ds38k/ds[+]
fjd1 has wing phenotype, enhanceable by ds38k/ds[+]
fjd1 has crossvein phenotype, enhanceable by ft1/ft[+]
fjd1 has wing phenotype, enhanceable by ft1/ft[+]
fjd1 has crossvein phenotype, enhanceable by ft1/ft1
fjd1 has wing phenotype, enhanceable by ft1/ft1
fjd1 has wing phenotype, enhanceable by ds1/dsUAO71
Enhancer of
Statement
Reference
fjd1/fj[+] is an enhancer of embryonic/larval cuticle phenotype of baz4
fjd1/fj[+] is an enhancer of embryonic/larval cuticle phenotype of bazG0484
fjd1/fjd1 is an enhancer of wing phenotype of ds1/dsUAO71
fjd1/fjd1 is an enhancer of crossvein phenotype of ft1
fjd1/fjd1 is an enhancer of wing phenotype of ft1
NOT Enhancer of
Statement
Reference
fjd1/fjd1 is a non-enhancer of wing hair phenotype of ds05142
Suppressor of
NOT Suppressor of
Statement
Reference
fjd1/fjd1 is a non-suppressor of wing hair phenotype of ds05142
Other
Additional Comments
Genetic Interactions
Statement
Reference
Hair polarity on fjd1, pk30 double mutant wings is more distal than the pk30 phenotype.
Adding fjp1/fjd1 has little effect on the wing area of dsUAO71/ds38k flies. Adding fjp1/fjd1 dramatically reduces the wing area of flies expressing dsAct5C.T:Avic\GFP-EGFP in a dsUAO71/ds38k background. Adding fjp1/fjd1 has no effect on the wing area of flies expressing dsSAx3.Act5C.T:Avic\GFP-EGFP in a dsUAO71/ds38k background. Adding fjp1/fjd1 has no effect on the wing area of flies expressing dsSDx3.Act5C.T:Avic\GFP-EGFP in a dsUAO71/ds38k background.
The cuticle phenotype of dead embryos derived from baz4/+ embryos derived from a cross of baz4/+ females to wild-type males is enhanced if the females also carry one copy of fjd1. The cuticle phenotype of dead embryos derived from bazG0484/+ embryos derived from a cross of bazG0484/+ females to wild-type males is enhanced if the females also carry one copy of fjd1.
The planar polarity phenotype of ds05142/ds05142 wings is not enhanced by fjd1/fjd1. fjd1/fjd1 dsScer\UAS.cTa ; Scer\GAL4αTub84B.PP flies have only mild wing planar polarity defects. The planar polarity defects seen in the wings of dsScer\UAS.cTa; Scer\GAL4salm-459.2 flies are midly enhanced by fjd1/fjd1 : polarity defects are seen outside the regions of the wing affected by dsScer\UAS.cTa; Scer\GAL4salm-459.2 alone. Planar cell polarity defects in the posterior compartment of ftScer\UAS.cMa; Scer\GAL4en-e16E flies are strongly suppressed by fjd1/fjd1.
ds38k fjN7/dsUAO71 fjd1 flies have disorganised ommatidial polarity; no obvious equator is present. Flies expressing both fjScer\UAS.cZa and dsScer\UAS.P\T.cSa under the control of Scer\GAL4αTub84B.PL in a ds38k fjN7/dsUAO71 fjd1 background have a disorganised ommatidial polarity; no obvious equator is present. Flies expressing dsScer\UAS.P\T.cSa under the control of Scer\GAL4αTub84B.PL in a ds38k fjN7/dsUAO71 fjd1 background have a disorganised ommatidial polarity; no obvious equator is present. Flies expressing fjScer\UAS.cZa under the control of Scer\GAL4αTub84B.PL in a ds38k fjN7/dsUAO71 fjd1 background have a disorganised ommatidial polarity; no obvious equator is present. The polarity reversals in the eye seen in flies expressing ftScer\UAS.P\T.cSa under the control of Scer\GAL4bi-omb3-GAL4 is strengthened in a fjN7/fjd1 background. Flies expressing both fjScer\UAS.cZa and dsScer\UAS.P\T.cSa under the control of Scer\GAL4αTub84B.PL in a ds38k fjN7/dsUAO71 fjd1 background have normal polarity throughout the wing blade with the exception of a small region along the anterior proximal margin of the wing, where a small swirl is often present. Flies expressing dsScer\UAS.P\T.cSa under the control of Scer\GAL4αTub84B.PL in a ds38k fjN7/dsUAO71 fjd1 background have normal polarity throughout the wing blade with the exception of a small region along the anterior proximal margin of the wing, where a small swirl is often present.
fjd1; ft1 double homozygotes have a much more severe wing shortening phenotype than either single mutant. The distance between anterior and posterior crossveins in the wings of these flies is reduced to almost zero. The wing shortening phenotype of fjd1 homozygotes is enhanced by ds38k/+, ds1/+ or by ft1/+. Unlike either single mutant, 'fjd1 ds1/fjd1 dsUAO71' flies completely lack a posterior crossvein and have defects in wing hair polarity. 16% of ommatidia in these double mutants are inverted compared to less than 1% in either single mutant.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
fjd1 is partially rescued by fj+t24
fjd1 is partially rescued by fj+t14
fjd1 is partially rescued by fjUn.t14
Comments
Expression of either fjD447Q.Scer\UAS, fjD454Q.Scer\UAS or fjD490Q.Scer\UAS under the control of Scer\GAL4αTub84B.PL does not rescue the reversal of hair polarity that is seen behind fjd1 clones in the dorsal adult abdomen.
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (22)