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General Information
Symbol
Dmel\ksrS-638
Species
D. melanogaster
Name
FlyBase ID
FBal0049701
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ksrS638
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    Nucleotide change:
    C5481551T
    Amino acid change:
    A696V | ksr-PA; A696V | ksr-PB; A696V | ksr-PC; A696V | ksr-PD
    Reported amino acid change:
    A696V
    Comment:
    One of two nucleotide substitutions in the ksr coding region in the allele.
    Comment:
    Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
    point mutation
    Nucleotide change:
    G5481531A
    Amino acid change:
    A703T | ksr-PA; A703T | ksr-PB; A703T | ksr-PC; A703T | ksr-PD
    Reported amino acid change:
    A703T
    Comment:
    One of two nucleotide substitutions in the ksr coding region in the allele. It falls within a highly conserved residue within the kinase subdomain II.
    Comment:
    Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Amino acid replacement: A696V. Amino acid replacement: A703T. A703 is a highly conserved residue within the kinase II subdomain II.
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    abdominal segment 8 & cephalopharyngeal skeleton | germ-line clone
    abdominal segment 9 & cephalopharyngeal skeleton | germ-line clone
    abdominal segment 10 & cephalopharyngeal skeleton | germ-line clone
    abdominal segment 11 & cephalopharyngeal skeleton | germ-line clone
    Detailed Description
    Statement
    Reference
    ksrS-638 embryos (as well as ksrS-638/ksrS-627 that show a stronger phenotype) derived from mothers bearing ksrS-638 mutant germline clones and thus lacking both maternal and zygotic ksr do not show any loss of muscle founder cells at late embryonic stages and only display rather mild visceral muscle phenotypes such as myotube stretching defects at stage 13/14 and incomplete midgut constriction at the end of embryogenesis.
    ksrS-638 homozygous clones in the dorsal air sac primordium grow more slowly than wild-type clones and never contribute to the tip of the primordium.
    Clonal analysis fails to produce clones of ksrS-638 suggesting ksr is required for cell proliferation or survival. Embryos derived from germline clones reveal severe terminal defects. They are missing posterior structures beyond the seventh abdominal segment and have collapsed head skeleton. Embryos derived from germline clones of ksrS-638 reveal severe terminal defects.
    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Suppressed by
    Statement
    Reference
    Enhancer of
    Statement
    Reference
    ksr[+]/ksrS-638 is an enhancer of phenotype of cswlf
    Suppressor of
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Dominantly suppresses the eye phenotype caused by C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 expressed under the control of Scer\GAL4GMR.PF. Completely dominantly suppresses the wing vein phenotype caused by C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 expressed under the control of Scer\GAL4dpp.blk1.
    The terminal defect phenotype seen in germline clones of ksrS-638 can be partially rescued by the injection of mRNA generated from phl::tor13D.hs.sev. Dominantly suppresses the rough eye phenotype of Ras85DV12.sev. Suppresses the rough eye phenotype of Ras85D::Src64BV12.ΔCAAX.sev. The terminal defects of embryos derived from homozygous ksrS-638 germline clones can be partially rescued by the injection of mRNA generated from phl::tor13D.hs.sev.
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (6)