FlyBase Allele Report: Dmel\msi[1]

General Information

Symbol

Dmel\msi¹

Species

D. melanogaster

Name

FlyBase ID

FBal0050164

Feature type

allele

Associated gene

Dmel\msi

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Nakamura et al., 1994)

Mutagen

Delta2-3 transposase

(Nakamura et al., 1994)

Progenitor genotype

P{PZ}msi⁹⁰⁰

(Nakamura et al., 1994)

Cytology

Description

Imprecise excision of an enhancer trap element, deletion of the entire element and 600bp of flanking genomic DNA (including the initiator codon).

(Nakamura et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult olfactory receptor neuron (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

adult olfactory receptor neuron | cell autonomous

(Landínez-Macías et al., 2021)

adult thoracic mechanosensory chaeta neuron (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

antennal lobe glomerulus VA7m (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

antennal lobe glomerulus VA7m | cell autonomous

(Landínez-Macías et al., 2021)

axon | adult stage | cell autonomous

(Landínez-Macías et al., 2021)

axonal growth cone | decreased number | pupal stage (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

axon collateral | absent | adult stage (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

axon collateral | absent | pupal stage (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

axon collateral | adult stage (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

axon collateral | pupal stage (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

axon terminus | adult stage (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

axon terminus | adult stage | decreased number (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

axon terminus | pupal stage (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

external sensory organ

(Okabe et al., 2001)

germline stem cell | somatic clone

(Siddall et al., 2006)

glial cell & macrochaeta

(Nakamura et al., 1994)

macrochaeta & scutum

(Nakamura et al., 1994)

male germline stem cell (with msi²)

(Siddall et al., 2012)

mechanosensory neuron of chaeta | pupal stage (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

microchaeta & scutum

(Nakamura et al., 1994)

neuron & macrochaeta

(Nakamura et al., 1994)

neuron & microchaeta

(Nakamura et al., 1994)

ommatidium

(Hirota et al., 1999)

presynapse | adult stage | decreased number (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

presynapse | adult stage | ectopic (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

presynapse | ectopic | pupal stage (with Df(3R)Exel6203)

(Landínez-Macías et al., 2021)

rhabdomere

(Hirota et al., 1999)

spermatogonium | somatic clone

(Siddall et al., 2006)

testis

(Siddall et al., 2006)

testis (with msi²)

(Siddall et al., 2006)

testis sheath muscle cell

(Siddall et al., 2006)

testis sheath muscle cell (with msi²)

(Siddall et al., 2006)

thecogen cell

(Nakamura et al., 1994)

thecogen cell & dorsal triple row

(Nakamura et al., 1994)

thecogen cell & medial triple row

(Nakamura et al., 1994)

thecogen cell & twin sensillum of margin 1

(Nakamura et al., 1994)

thecogen cell & twin sensillum of margin 2

(Nakamura et al., 1994)

tormogen cell

(Nakamura et al., 1994)

trichogen cell | increased number

(Okabe et al., 2001)

Detailed Description

Statement

Reference

In msi¹/Df(3R)Exel6203 adults, thoracic mechanosensory neurons exhibit decreased connectivity: frequently terminal synaptic arborizations are lost and presynapse number is decreased; frequently the primary axon branch fails to reach the contralateral side of the CNS and is arrested at the midline/on the ipsilateral side; or frequently, the contralateral branch is missing; however, there are ectopic presynapses in the posterior branch. Branch and synapse patterning are also affected at early stages of axon targeting during pupal development: frequently, the contralateral branch is missing; frequently there are ectopic presynapses in the posterior branch; at 45-55h apf, filopodial protrusions sprout when the primary branch projects toward the contralateral side; at 65-75h apf, however, the contralateral branch almost lacks both satellite growth cones and the filopodial protrusions.

In msi¹/Df(3R)Exel6203 adults, the axons of or10a-expressing ORNs mistarget to the VA7m glomerulus.

msi¹ homozygous or10a ORN clones in a mostly heterozygous adult mistarget to the VA7m glomerulus.

(Landínez-Macías et al., 2021)

msi¹ and msi¹/msi² mutant testes exhibit fewer brightly stained cells at the apex of the testis, indicative of a loss of early germ cells. msi¹ mutant testis also exhibit a swelling of the apical region in ~90% of testes analyzed.

msi¹ mutant third-instar larvae exhibit a hub in 97% of testes analysed and exhibit milder morphological defects than those observed in pharate adults.

In msi¹ mutants, there are on average 3.9 germ stem cells per testis, compared to 8.4 per testis in wild-type.

Germline stem cell clones homozygous for msi¹ are found at the same frequency as control clones 2 days after clone induction. Although wild-type clones are maintained at similar levels over time, msi¹ stem cell clones are not maintained at the same frequency. At 8 days after induction, msi¹ mutant clones are observed significantly less frequently than control wild-type germ line stem cells of the same age.

msi¹ spermatogonia clones are found in 47% of testes 5 days after clone induction, this is a similar number to that found in control clones.

Acridine orange staining does not reveal the presence of any excess dying cells in msi¹ mutant clones. In 15% of cases, msi¹ spermatogonial clones are found 8 days after induction in the absence of msi¹ germline stem cells, indicating that msi¹ germline stem cells can differentiate into spermatogonial cells without regeneration of germline stem cells.

In msi¹ mutants, early round spermatids commonly contain two or four nuclei and a large mitochondrial derivative, indicating that one or both meiotic cytokinetic divisions have failed. These mutants often display haploid nuclei of different sizes, resulting from errors in chromosome segregation during meiosis.

(Siddall et al., 2006)

Mutants show a double bristle phenotype.

(Okabe et al., 2001)

Homozygotes show a low penetrance phenotype of abnormal ommatidia with deformed rhabdomeres and abnormal ommatidial orientation. The number of photoreceptor cells is normal, in affected ommatidia.

(Hirota et al., 1999)

Lethality occurs during late pupal and early adult stages. The total number of sensilla is similar to wild type but the number of outer support cells (sockets and shafts) is variable. Microchaetae on the notum have variable phenotypes, some having up to 4 sockets and no shaft. The presence of extra support cells is correlated to the absence of neurons. Macrochaetae also display variability, the most frequent phenotype is two shafts and one socket. Macrochaetae with additional support cells frequently had one or more neurons but did not typically contain glial cells. Anterior wing margin has extra support cells in the singly innervated bristles and multiple innervated bristles and extra outer support cells in the twin campaniform sensilla.

(Nakamura et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

msi¹/Df(3R)Exel6203 has abnormal neuroanatomy | adult stage phenotype, enhanceable by Ptp69D¹/Ptp69D[+]

(Landínez-Macías et al., 2021)

msi¹/Df(3R)Exel6203 has decreased size | adult stage phenotype, enhanceable by Ptp69D¹/Ptp69D[+]

(Landínez-Macías et al., 2021)

msi¹ has visible | recessive phenotype, enhanceable by sina³/sina²

(Okabe et al., 2001)

msi¹ has visible phenotype, enhanceable by sina³/sina²

(Hirota et al., 1999)

Suppressed by

Statement

Reference

msi¹/Df(3R)Exel6203 has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Ptp69D^{UAS.UTRs.mCherry}/Scer\GAL4^pnr-MD237

(Landínez-Macías et al., 2021)

msi¹ has visible | recessive phenotype, suppressible by ttk[+]/ttk^osn

(Okabe et al., 2001)

NOT suppressed by

Statement

Reference

msi¹ has visible | recessive phenotype, non-suppressible by N^ECN.UAS/Scer\GAL4^sca-109-68

(Okabe et al., 2001)

Enhancer of

Statement

Reference

msi[+]/msi¹ is an enhancer of visible | dominant phenotype of N^55e11

(Kankel et al., 2007)

msi¹/msi¹ is an enhancer of visible phenotype of sina³/sina²

(Okabe et al., 2001)

NOT Enhancer of

Statement

Reference

msi¹ is a non-enhancer of abnormal neuroanatomy phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

NOT Suppressor of

Statement

Reference

msi¹ is a non-suppressor of abnormal neuroanatomy phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

Other

Statement

Reference

Ptp69D¹, msi[+]/msi¹ has abnormal neuroanatomy | adult stage phenotype

(Landínez-Macías et al., 2021)

Ptp69D¹, msi[+]/msi¹ has decreased size | adult stage phenotype

(Landínez-Macías et al., 2021)

N^ECN.UAS, Scer\GAL4^sca-109-68, msi¹ has visible phenotype

(Okabe et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

msi¹/Df(3R)Exel6203 has adult thoracic mechanosensory chaeta neuron phenotype, enhanceable by Ptp69D¹/Ptp69D[+]

(Landínez-Macías et al., 2021)

msi¹/Df(3R)Exel6203 has axon collateral | adult stage phenotype, enhanceable by Ptp69D¹/Ptp69D[+]

(Landínez-Macías et al., 2021)

msi¹/Df(3R)Exel6203 has axon terminus | adult stage | decreased number phenotype, enhanceable by Ptp69D¹/Ptp69D[+]

(Landínez-Macías et al., 2021)

msi¹/Df(3R)Exel6203 has presynapse | adult stage | decreased number phenotype, enhanceable by Ptp69D¹/Ptp69D[+]

(Landínez-Macías et al., 2021)

msi¹ has external sensory organ phenotype, enhanceable by sina³/sina²

(Okabe et al., 2001)

msi¹ has ommatidium phenotype, enhanceable by sina³/sina²

(Hirota et al., 1999)

NOT Enhanced by

Statement

Reference

msi²/msi¹ has male germline stem cell phenotype, non-enhanceable by Rbp6¹

(Siddall et al., 2012)

Suppressed by

Statement

Reference

msi¹/Df(3R)Exel6203 has adult thoracic mechanosensory chaeta neuron phenotype, suppressible | partially by Ptp69D^{UAS.UTRs.mCherry}/Scer\GAL4^pnr-MD237

(Landínez-Macías et al., 2021)

msi¹/Df(3R)Exel6203 has axon collateral | adult stage | absent phenotype, suppressible | partially by Ptp69D^{UAS.UTRs.mCherry}/Scer\GAL4^pnr-MD237

(Landínez-Macías et al., 2021)

msi¹/Df(3R)Exel6203 has axon collateral | adult stage phenotype, suppressible | partially by Ptp69D^{UAS.UTRs.mCherry}/Scer\GAL4^pnr-MD237

(Landínez-Macías et al., 2021)

msi¹/Df(3R)Exel6203 has axon terminus | adult stage | decreased number phenotype, suppressible | partially by Ptp69D^{UAS.UTRs.mCherry}/Scer\GAL4^pnr-MD237

(Landínez-Macías et al., 2021)

msi¹, sina³/sina² has photoreceptor cell R1 | third instar larval stage phenotype, suppressible by ttk^GD4414/Scer\GAL4^lz-gal4

(Siddall et al., 2009)

msi¹, sina³/sina² has photoreceptor cell R6 | third instar larval stage phenotype, suppressible by ttk^GD4414/Scer\GAL4^lz-gal4

(Siddall et al., 2009)

msi¹, sina³/sina² has photoreceptor cell R7 | third instar larval stage phenotype, suppressible by ttk^GD4414/Scer\GAL4^lz-gal4

(Siddall et al., 2009)

msi¹ has trichogen cell | increased number phenotype, suppressible by ttk[+]/ttk^osn

(Okabe et al., 2001)

msi¹ has phenotype, suppressible by ttk^osn

(Hirota et al., 1999)

msi¹, sina³/sina² has ommatidium phenotype, suppressible by ttk^osn

(Hirota et al., 1999)

msi¹, sina³/sina² has rhabdomere phenotype, suppressible by ttk^osn

(Hirota et al., 1999)

msi¹, sina³/sina² has cone cell phenotype, suppressible by ttk^osn

(Hirota et al., 1999)

NOT suppressed by

Statement

Reference

msi²/msi¹ has male germline stem cell phenotype, non-suppressible by Rbp6¹

(Siddall et al., 2012)

msi¹ has external sensory organ phenotype, non-suppressible by N^ECN.UAS/Scer\GAL4^sca-109-68

(Okabe et al., 2001)

msi¹ has trichogen cell | increased number phenotype, non-suppressible by N^ECN.UAS/Scer\GAL4^sca-109-68

(Okabe et al., 2001)

Enhancer of

Statement

Reference

msi¹/msi¹ is an enhancer of photoreceptor cell R1 | third instar larval stage phenotype of sina³/sina²

(Siddall et al., 2009)

msi¹/msi¹ is an enhancer of photoreceptor cell R6 | third instar larval stage phenotype of sina³/sina²

(Siddall et al., 2009)

msi¹/msi¹ is an enhancer of photoreceptor cell R7 | third instar larval stage phenotype of sina³/sina²

(Siddall et al., 2009)

msi¹ is an enhancer of egg phenotype of Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C}

(Geng and MacDonald, 2007)

msi¹ is an enhancer of egg chorion phenotype of Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C}

(Geng and MacDonald, 2007)

msi[+]/msi¹ is an enhancer of wing phenotype of N^55e11

(Kankel et al., 2007)

msi¹/msi¹ is an enhancer of external sensory organ phenotype of sina³/sina²

(Okabe et al., 2001)

NOT Suppressor of

Statement

Reference

msi¹/msi¹ is a non-suppressor of external sensory organ precursor cell IIb | increased number phenotype of ttk^osn

(Okabe et al., 2001)

msi¹/msi¹ is a non-suppressor of external sensory organ precursor cell IIa phenotype of ttk^osn

(Okabe et al., 2001)

Other

Statement

Reference

Ptp69D¹, msi[+]/msi¹ has axon collateral | adult stage phenotype

(Landínez-Macías et al., 2021)

Ptp69D¹, msi[+]/msi¹ has adult thoracic mechanosensory chaeta neuron phenotype

(Landínez-Macías et al., 2021)

Ptp69D¹, msi[+]/msi¹ has axon terminus | adult stage | decreased number phenotype

(Landínez-Macías et al., 2021)

Ptp69D¹, msi[+]/msi¹ has presynapse | adult stage | decreased number phenotype

(Landínez-Macías et al., 2021)

N^ECN.UAS, Scer\GAL4^sca-109-68, msi¹ has external sensory organ phenotype

(Okabe et al., 2001)

N^ECN.UAS, Scer\GAL4^sca-109-68, msi¹ has trichogen cell phenotype

(Okabe et al., 2001)

N^ECN.UAS, Scer\GAL4^sca-109-68, msi¹ has eo neuron phenotype

(Okabe et al., 2001)

msi¹, sina³/sina², ttk^osn has ommatidium phenotype

(Hirota et al., 1999)

msi¹, sina³/sina², ttk^osn has cone cell phenotype

(Hirota et al., 1999)

msi¹, sina³/sina², ttk^osn has rhabdomere phenotype

(Hirota et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

In Ptp69D¹/+, msi¹/+ adults, thoracic mechanosensory neurons exhibit decreased connectivity: reduced contralateral branch synaptic arborizations; and synapse numbers.

(Landínez-Macías et al., 2021)

A msi¹ background weakly enhances the dorsalisation phenotype observed in Imp^Scer\UAS.cGa overexpression (Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) mutants.

(Geng and MacDonald, 2007)

Dominantly enhances the Df(1)N-54l9/+ and N^55e11/+ wing nicking phenotypes.

(Kankel et al., 2007)

N^ECN.Scer\UAS (Scer\GAL4^sca-109-68), msi¹ double mutants show a dense double bristle phenotype. The IIb precursor takes the non-neuronal fate as there are no neurons in the subepidermal layer.

(Okabe et al., 2001)

Double mutants of sina²/sina³ with msi¹ show a synergistic, not additive, eye phenotype. Ommatidial arrays are disturbed, eyes consequently roughened and ommatidia do not contain more than five rhabdomeres. Cone cell array is disturbed. 30% of the ommatidia of sina² msi¹ ttk^osn/sina³ msi¹ show the normal number and arrangement of photoreceptor cells. The cone cell defects of sina² msi¹/sina³ msi¹ are also suppressed by ttk^osn.

(Hirota et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

msi¹ is rescued by msi^+t10

(Hirota et al., 1999)

msi¹ is rescued by msi^+t14.3

(Nakamura et al., 1994)

Not rescued by

msi¹ is not rescued by msi^A.B

(Hirota et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

4160

107603

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

msi¹

(Siddall et al., 2022, Landínez-Macías et al., 2021, Berson et al., 2019, Siddall et al., 2012, Siddall et al., 2009, Geng and MacDonald, 2007, Kankel et al., 2007, Sofola et al., 2007, Siddall et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (13)

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