FlyBase Allele Report: Dmel\pav[B200]

General Information

Symbol

Dmel\pav^B200

Species

D. melanogaster

Name

FlyBase ID

FBal0050242

Feature type

allele

Associated gene

Dmel\pav

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Gregory et al., 2007, Salzberg et al., 1994)

Mutagen

ethyl methanesulfonate

(Salzberg et al., 1994)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal ventral denticle belt | embryonic stage

(Jones et al., 2010)

abdominal ventral denticle belt | embryonic stage (with Df(3L)GN24)

(Jones et al., 2010)

centrosome | embryonic cycle 16

(Adams et al., 1998)

embryonic hypodermal muscle cell

(Yang et al., 2022)

larval somatic muscle cell

(Guerin and Kramer, 2009)

microtubule

(Somers and Saint, 2003)

mitotic domain 1 | embryonic cycle 16

(Adams et al., 1998)

mushroom body & axon | somatic clone

(Goldstein et al., 2005)

neuron & peripheral nervous system

(Salzberg et al., 1994)

spindle | embryonic cycle 16

(Adams et al., 1998)

Detailed Description

Statement

Reference

In pav^B200 embryos, somatic muscles show attachment or elongation defects.

(Yang et al., 2022)

pav^B200 mutant embryos have epidermal pattern defects.

(Jones et al., 2010)

Homozygous embryos show defects in muscle attachment site selection. The mutant muscles no longer show uniform microtubule polarity at stages 14/15 and 16, in contrast to wild type.

(Guerin and Kramer, 2009)

Single cell mutant clones in the mushroom body neuroblasts made in newly hatched larvae, causes arrest of neuroblast proliferation and prevents the continued generation of later born α'/β' and α/β neurons. Enlarged cells also indicated cytokinesis defects. Single cell mutant clones in the mushroom body neurons are grossly normal in their axonal and dendritic projections.

(Goldstein et al., 2005)

Mitotic cycle 16 cells in pav^B200 mutant embryos are depleted for bundled microtubules.

(Somers and Saint, 2003)

Homozygous or hemizygous embryos show cytokinesis defects during mitosis 16. The cell membrane fails to invaginate during telophase of cycle 16 in some cases, producing binucleate cells. The nuclei of these cells appear to enter interphase 17, as the nuclear laminae reform around both nuclei. Cells with multipolar spindles and multiple centrosomes that are associated with a tetraploid mass of chromatin are seen at a very low frequency. The central spindle appears to contain fewer bundles of microtubules at telophase of cycle 16 than in wild-type embryos. Peripheral spindle microtubules do not become constricted, in contrast to wild-type.

(Adams et al., 1998)

Defect in embryonic PNS development: defect in cell division causing a reduction in the number of neurons and an increase in size of the remaining neurons.

(Salzberg et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

pav[+]/pav^B200 is an enhancer of abnormal meiotic cell cycle phenotype of Df(2L)ED1305/nesd^EY08338

(Montembault et al., 2010)

pav[+]/pav^B200 is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, pbl^{ΔDH497-549.UAS}

(Gregory et al., 2007)

pav^B200 is an enhancer of visible phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Collins and Cohen, 2005)

Suppressor of

Statement

Reference

pav[+]/pav^B200 is a suppressor of lethal | recessive | embryonic stage phenotype of arm⁸

(Jones et al., 2010)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

pav^B200 has abdominal ventral denticle belt | embryonic stage phenotype, non-enhanceable by tum^AR2

(Jones et al., 2010)

NOT suppressed by

Statement

Reference

pav^B200 has abdominal ventral denticle belt | embryonic stage phenotype, non-suppressible by tum^AR2

(Jones et al., 2010)

Enhancer of

Statement

Reference

pav[+]/pav^B200 is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, pbl^{ΔDH497-549.UAS}

(Gregory et al., 2007)

pav^B200 is an enhancer of wing phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Collins and Cohen, 2005)

NOT Enhancer of

Statement

Reference

pav^B200/pav^B200 is a non-enhancer of embryonic hypodermal muscle cell | embryonic stage phenotype of tum^DH15

(Yang et al., 2022)

pav[+]/pav^B200 is a non-enhancer of abdominal ventral denticle belt | embryonic stage phenotype of Scer\GAL4^e22c, pan^ΔN.UAS

(Jones et al., 2010)

pav[+]/pav^B200 is a non-enhancer of abdominal ventral denticle belt | embryonic stage phenotype of Scer\GAL4^e22c, arm⁴

(Jones et al., 2010)

Suppressor of

Statement

Reference

pav[+]/pav^B200 is a suppressor | partially of mechanosensory medial triple row bristle | decreased number phenotype of Arf6¹

(Marcetteau et al., 2021)

pav[+]/pav^B200 is a suppressor | partially of mechanosensory medial triple row bristle | decreased number phenotype of Arf6^GX16w-

(Marcetteau et al., 2021)

pav[+]/pav^B200 is a suppressor of abdominal ventral denticle belt | embryonic stage phenotype of arm⁸

(Jones et al., 2010)

NOT Suppressor of

Statement

Reference

pav[+]/pav^B200 is a non-suppressor of abdominal ventral denticle belt | embryonic stage phenotype of Scer\GAL4^e22c, pan^ΔN.UAS

(Jones et al., 2010)

pav[+]/pav^B200 is a non-suppressor of abdominal ventral denticle belt | embryonic stage phenotype of Scer\GAL4^e22c, arm⁴

(Jones et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The phenotype of tum^AR2; pav^B200 double mutant embryos does not show significant differences from the tum^AR2 and pav^B200 single mutant phenotypes.

The elimination of naked cuticle observed in Scer\GAL4^e22c > pan^ΔN.Scer\UAS embryos is unchanged in a pav^B200/+ mutant background.

The elimination of naked cuticle observed in arm⁴ mutant embryos is unchanged in a pav^B200/+ mutant background.

Heterozygosity for pav^B200 restores areas of naked cuticle between the belts to almost wide-type levels, and many more arm⁸ mutants are able to hatch into the first instar stage.

(Jones et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

pav²⁰⁴⁶

(Collins and Cohen, 2005)

pav⁸³¹

(Collins and Cohen, 2005)

pav⁹⁶³

(Collins and Cohen, 2005)

Rescued by

pav^B200 is rescued by Scer\GAL4^e22c/pav^UASp.mGFP6

(Jones et al., 2010)

pav⁸³¹/pav^B200 is rescued by pav^+t10.5

(Collins and Cohen, 2005)

pav²⁰⁴⁶/pav^B200 is rescued by pav^+t10.5

(Collins and Cohen, 2005)

pav^M547/pav^B200 is rescued by pav^+t10.5

(Adams et al., 1998)

Not rescued by

pav^B200 is not rescued by pav^{NLS4-7*.UASp.mGFP6}/Scer\GAL4^e22c

(Jones et al., 2010)

pav^M547/pav^B200 is not rescued by pav^RC2

(Adams et al., 1998)

Comments

Expression of pav^{Scer\UAS.P\T.T:Avic\GFP-m6} driven by Scer\GAL4^e22c rescues the epidermal pattern defects of pav^B200 mutants.

Expression of pav^{NLS4-7*.Scer\UAS.P\T.T:Avic\GFP-m6} driven by Scer\GAL4^e22c fails to rescue the epidermal pattern defects of pav^B200 mutants.

(Jones et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4384

y¹ w¹; P{lacW}64A pav^B200 Diap1^th-1 st¹ cu¹ sr¹ e^s ca¹/TM6B, P{iab-2(1.7)lacZ}6B, Tb¹

Kyoto

107716

y¹ w¹; P{lacW}64A pav^B200 Diap1^th-1 st¹ cu¹ sr¹ e^s ca¹/TM6B, P{iab-2(1.7)lacZ}6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

pav^B200

(Yang et al., 2022, Marcetteau et al., 2021, Thomas et al., 2021, Nakamura et al., 2020, Das et al., 2016, Unhavaithaya et al., 2013, Jones et al., 2010, Montembault et al., 2010, Guerin and Kramer, 2009, Christensen et al., 2008.7.11, Christensen et al., 2007.10.29, Dyer et al., 2007, Gregory et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (19)

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