FB2025_01 , released February 20, 2025
Allele: Dmel\sa2
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General Information
Symbol
Dmel\sa2
Species
D. melanogaster
Name
FlyBase ID
FBal0050456
Feature type
allele
Associated gene
Dmel\sa
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Lin et al., 1996)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q109term.

(Hiller et al., 2004)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:21,406,309..21,406,309 [-]
Nucleotide change:

C21406309T

Amino acid change:

Q109term | sa-PA

Reported amino acid change:

Q109term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lin et al., 1996)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      sa1/sa2 adult heterozygote males show morphological defects of the nuclear lamina in stage S4-S6 spermatocytes (the lamina has distinctly more ballon-like shape, lacking the deformations that correlate with chromosome distribution seen in wild-type), while in stage S3 the lamina is of wild-type appearance.

      (Trost et al., 2016)

      sa1/sa2 mutant spermatocytes exhibit meiotic arrest and other defects, including bivalent chromosomes condensing to spherical shapes away from the nuclear periphery, and failure of the nucleolus to fully break down.

      (Lu and Fuller, 2015)

      sa1/sa2 males are sterile due to arrested spermatogenesis at spermatocyte stage.

      (Hiller et al., 2004)

      Early stages of spermatogenesis up through mature primary spermatocytes are present and normal. Postmeiotic stages are completely absent. Mutant testes have an abundance of mature primary spermatocytes with large, round nuclei and prominent nucleoli. Spermatocytes arrest in the G2/M transition of meiosis I with partial chromosome condensation.

      (Lin et al., 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      sa2/sa1 is rescued by saEGFP

      (Chen et al., 2005)

      sa2/sa1 is rescued by saTag:HA

      (Chen et al., 2005)

      sa2/sa1 is rescued by sa+t3.2

      (Hiller et al., 2004)
      Comments

      sa+t3.2 fully rescues the spermatocyte arrest and male sterility phenotypes of sa1/sa2 males.

      (Hiller et al., 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      References (11)