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General Information
Symbol
Dmel\Diap15
Species
D. melanogaster
Name
FlyBase ID
FBal0050595
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
th5, th5
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    Nucleotide change:

    G16039171A

    Amino acid change:

    W273term | Diap1-PA; W273term | Diap1-PB; W273term | Diap1-PC; W273term | Diap1-PD; W273term | Diap1-PE; W273term | Diap1-PF

    Reported amino acid change:

    W273term

    Comment:

    G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Mutation is within the second BIR domain.

    Amino acid replacement: W273term.

    The premature stop codon is near the middle of the second BIR domain.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    th5/+ flies show near normal eye phenotype.

    14% of th5/th21-2s egg chambers have border follicle cell migration defects, 25% have extra follicle cells and 3% have more than 15 nurse cells. 3% of th5/th21-2s egg chambers show polarity defects. Homozygous follicle cells clones result in border follicle cell migration defects.

    th5 homozygotes progress normally through gastrulation but arrest at the beginning of germ band extension, and within 90 min thereafter, adopt a characteristic morphology reflecting catastrophic events associated with widespread and synchronous apoptosis and arrest at stage 7.

    Heterozygotes are phenotypically wild type.

    Embryos show increased cell death as assessed by TUNEL staining. The onset of this apoptosis appears several hours after development arrests.

    Epidermis of embryos does not form a cuticle. Homozygotes and hemizygotes undergo normal cellularization and initial gastrulation. During germ band extension all morphogenetic movements cease, cells adopt a rounded morphology (suggesting disruption if intercellular adhesion) and the yolk cell fragments and moves to the surface of the embryo. Cephalic furrow regresses. Surface of cells contain blebs and vesicular fragments characteristic of dying cells. Embryos show a significant increase in TUNEL-positive cells, but no significant change in acridine orange staining. Double mutants with Df(3L)H99 show a phenotype indistinguishable from that of th5 mutants alone.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressed by
    NOT suppressed by
    Enhancer of
    Statement
    Reference
    Other
    Phenotype Manifest In
    Suppressed by
    NOT suppressed by
    Enhancer of
    Statement
    Reference

    Diap15 is an enhancer of eye phenotype of shtd1

    Diap15 is an enhancer of ommatidium phenotype of shtd1

    Diap15 is an enhancer of phenotype of rprGMR.PW

    Diap15 is an enhancer of phenotype of hidGMR.PG/WGMR.PG

    Diap15/th[+] is an enhancer of eye phenotype of DroncGMR.PH

    Diap15 is an enhancer of eye phenotype of grimGMR.PC

    Diap15 is an enhancer of eye phenotype of rprGMR.PW

    Diap15 is an enhancer of eye phenotype of rprGMR.PH

    Suppressor of
    Statement
    Reference
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    A th5 mutant background enhances the moderately rough eye phenotype observed upon expression of mblC.Scer\UAS under the control of Scer\GAL4hs.2sev.

    80% of chic01320/chick13321 ; th5/+ egg chambers have border follicle cell migration defects. 77% of chic01320/chic221 ; th5/+ egg chambers have border follicle cell migration defects.

    The addition of WGMR.PG to th5 heterozygotes leads to an enhancement of cell killing. The subsequent addition of ArkCD4/ArkCD4 suppresses that enhancement. The developmental arrest phenotype is also suppressed, embryos developing through stage 10. In about 5% of cases although no cell death is seen, arrest at stage 7 is still seen.

    Homozygous th5 embryos that express BacA\p35Scer\UAS.cHa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 undergo normal morphogenesis until stage 11, after which massive cell death resumes.

    Addition of this allele strongly enhances the phenotype seen in rprGMR.PW and WGMR.PG flies.

    Flies expressing intermediate levels of NcGMR.PH and also heterozygous for th5 have small eyes.

    Xenogenetic Interactions
    Statement
    Reference

    The Hsp60DdsRNA.Sym.Scer\UAS-mediated suppression of the eye degeneration phenotype caused by Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpression via Scer\GAL4GMR.PU is not much affected by heterozygosity for th5.

    The enhancement of the eye degeneration resulting from the overexpression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU in a th5/+ background is not suppressed when Hsp60DdsRNA.Sym.Scer\UAS is co-expressed.

    Heterozygosity for th5 enhances the eye degeneration resulting from the overexpression of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU.

    Heterozygosity for th5 enhances the eye degeneration resulting from the overexpression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU.

    Homozygous th5 embryos that express BacA\p35Scer\UAS.cHa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 undergo normal morphogenesis until stage 11, after which massive cell death resumes.

    Complementation and Rescue Data
    Fails to complement
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    Comments
    Comments

    grimGMR.PC-induced apoptosis is enhanced by th5 to a slightly greater extent than by a deletion of th suggesting that a single complete BIR may have slight dominant negative properties.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (5)
    References (18)