FB2025_01 , released February 20, 2025
Allele: Dmel\tkvQ253D.UAS.cNc
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General Information
Symbol
Dmel\tkvQ253D.UAS.cNc
Species
D. melanogaster
Name
FlyBase ID
FBal0050625
Feature type
allele
Associated gene
Dmel\tkv
Associated Insertion(s)
Carried in Construct
P{UAS(-FRT)tkv.Q253D.Nc}
Also Known As
TKVQD, UAS-tkvQD
Key Links
Transgenic product class
missense_variant
(Nellen et al., 1996)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Nellen et al., 1996)
Mutagen
FLPase
(Greenwood and Struhl, 1999, Abu-Shaar and Mann, 1998, Lecuit and Cohen, 1997, Nellen et al., 1996)
Progenitor genotype
tkvQ253D.FRT.UAS
(Greenwood and Struhl, 1999, Abu-Shaar and Mann, 1998, Gonzalez-Crespo et al., 1998, Lecuit and Cohen, 1997, Burke and Basler, 1996, Nellen et al., 1996)
Carried in construct
P{UAS(-FRT)tkv.Q253D.Nc}
Cytology
Description

FRT cassette has been excised, juxtaposing UAS regulatory sequences with tkv coding sequences.

(Nellen et al., 1996)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
tkv
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of tkvQ253D.Scer\UAS.cNc under the control of Scer\GAL4GMR.PF has no significant effect on eye size.

      Wing disc clones expressing tkvQ253D.Scer\UAS.cNc under the control of Scer\GAL4Scer\FRT.Act5C induce growth enhancement.

      (Oh and Irvine, 2011)

      Somatic clones of tkvQ253D.Scer\UAS.cNc; Scer\GAL4Act5C.Switch.PR cells in the mid third instar wing disc exhibit only autonomous effects on dpp signalling, but cause increases in cell proliferation both autonomously and non-autonomously. (Note - Each of the following results refers to clones in mid-third instar wing discs which differ): In the absence of RU486 treatment there is no increase in S-phase cells (BrdU incorporation) in or adjacent to these clones. However, increased BrdU incorporation is apparent within lateral clones following exposure to RU486 (20 μg/ml in food). This response is first seen in some clones after 5 hours exposure to RU486, but is more intense and seen in more clones after 12-14 hours exposure and is still apparent after 50 hours of exposure. Non-autonomous stimulation of BrdU incorporation is weakly apparent adjacent to some of these clones by 5 hours after exposure. This non-autonomous effect can be seen adjacent to both medial and lateral clones, but is most intense adjacent to lateral clones. The effect increases with time of exposure, peaking after about 12-14 hours of exposure. It remains high until 18-24 hours of exposure. After 24 hours exposure it is no longer apparent adjacent to most medial clones, but is retained adjacent to lateral clones although it is lost there by 50 hours of exposure. There is also a small but significant increase in the number of cells in M-phase adjacent to these clones after 15 hours exposure. These clones are associated with some increase in apoptosis, but only from around 35 hours after RU486 exposure. Increased cell proliferation prior to 35 hours is therefore not a secondary consequence of increased apoptosis. If a lower dose of RU486 is used (1 μg/ml) increased BrdU incorporation is limited to cells adjacent to lateral clones.

      The wing discs tkvQ253D.Scer\UAS.cNc third instar larvae carrying the steroid dependent ubiquitous driver Scer\GAL4Act5C.Switch.PR exposed to RU486 for 18 hours (20 μg/ml in food) have increased numbers of cells in S-phase and M-phase laterally, but decreased numbers medially. The lateral increase in S-phase is especially pronounced close to the dorsal-ventral compartment boundary. If a much lower concentration of RU486 is used (1 μg/ml), then both of medial decrease and lateral increase in S-phase cell numbers are are much weaker.

      (Rogulja and Irvine, 2005)

      When tkvQ253D.Scer\UAS.cNc (driven by Scer\GAL4Act5C.PP) is induced in somatic clones in early second instar larvae, clones in the wing disc are made that are several times larger with smoother borders than control clones. This phenotype is stronger in lateral areas of the disc - approximately half of the lateral clones are completely round and bulge out of the disc epithelium, generating extra folds around them. This is due to the cells proliferating 10-20% faster than wild-type cells apparently preferentially promoting G1/S progression. tkvQ253D.Scer\UAS.cNc cells have a shortened G1 phase. Clonal expression of tkvQ253D.Scer\UAS.cNc (driven by Scer\GAL4Act5C.PP) frequently causes lethality during pupal stages, and those animals that do eclose show a variety of wing phenotypes, including extra vein tissue, vein loss and notches in the wing. Some have outgrowths in the wing.

      (Martin-Castellanos and Edgar, 2002)

      Clones of cells in the eye disc within or behind the morphogenetic furrow expressing tkvQ253D.Scer\UAS.cNc under the control of Scer\GAL4arm.PS appear to develop normally in terms of photoreceptor differentiation.

      (Greenwood and Struhl, 1999)

      When expressed from Scer\GAL4C-765, mutant clones induced in the wing disc do not affect the size or general shape of the wing discs, but do grow faster than cells in dppScer\UAS.cNa clones, eventually causing a bulge in the epithelium.

      (Burke and Basler, 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4FRT.Act5C, tkvQ253D.UAS.cNc has increased cell number | somatic clone phenotype, suppressible by ykiB5

      (Oh and Irvine, 2011)

      Scer\GAL4Act5C.PP, tkvQ253D.UAS.cNc has increased cell number | somatic clone phenotype, suppressible by vg83b27-R

      (Martin-Castellanos and Edgar, 2002)

      Scer\GAL4Act5C.PP, tkvQ253D.UAS.cNc has increased cell number | somatic clone phenotype, suppressible by Pi3K21BUAS.Δ.Tag:HA, Scer\GAL4Act5C.PP

      (Martin-Castellanos and Edgar, 2002)

      Scer\GAL4Act5C.PP, tkvQ253D.UAS.cNc has increased cell number | somatic clone phenotype, suppressible by RbfUAS.cDa, Scer\GAL4Act5C.PP

      (Martin-Castellanos and Edgar, 2002)

      Scer\GAL4Act5C.PP, tkvQ253D.UAS.cNc has increased occurrence of cell division | somatic clone | larval stage phenotype, suppressible by RbfUAS.cDa, Scer\GAL4Act5C.PP

      (Martin-Castellanos and Edgar, 2002)

      Scer\GAL4Act5C.PP, tkvQ253D.UAS.cNc has increased mortality | somatic clone | pupal stage phenotype, suppressible by RbfUAS.cDa, Scer\GAL4Act5C.PP

      (Martin-Castellanos and Edgar, 2002)

      Scer\GAL4Act5C.PP, tkvQ253D.UAS.cNc has neoplasia | somatic clone | adult stage phenotype, suppressible by RbfUAS.cDa, Scer\GAL4Act5C.PP

      (Martin-Castellanos and Edgar, 2002)

      Scer\GAL4Act5C.PP, tkvQ253D.UAS.cNc has increased occurrence of cell division | somatic clone | larval stage phenotype, suppressible by Pi3K21BUAS.Δ.Tag:HA, Scer\GAL4Act5C.PP

      (Martin-Castellanos and Edgar, 2002)

      Scer\GAL4Act5C.PP, tkvQ253D.UAS.cNc has increased occurrence of cell division | somatic clone | larval stage phenotype, suppressible by vg83b27-R

      (Martin-Castellanos and Edgar, 2002)
      NOT suppressed by
      Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of tkvQ253D.Scer\UAS.cNc enhances the eye overgrowth seen when ykiS111A.S250A.Scer\UAS.T:SV5\V5 is expressed under the control of Scer\GAL4GMR.PF.

      Co-expression of MadScer\UAS.T:Zzzz\FLAG and tkvQ253D.Scer\UAS.cNc in wing disc clones under the control of Scer\GAL4Scer\FRT.Act5C modestly enhances growth in lateral regions.

      Expression of ykiS111A.S250A.Scer\UAS.T:SV5\V5 enhances the overgrowth seen in wing disc clones expressing MadScer\UAS.T:Zzzz\FLAG and tkvQ253D.Scer\UAS.cNc under the control of Scer\GAL4Scer\FRT.Act5C. The phenotype seen in stronger than is seen when ykiS111A.S250A.Scer\UAS.T:SV5\V5 is expressed alone.

      A ykiB5 mutant background suppresses the overgrowth seen when tkvQ253D.Scer\UAS.cNc is expressed in wing disc clones under the control of Scer\GAL4Scer\FRT.Act5C. As in ykiB5 mutants alone, very few clones are recoverable.

      Co-expression of tkvQ253D.Scer\UAS.cNc and MadScer\UAS.T:Zzzz\FLAG does not suppress the growth and survival defects seen when brkScer\UAS.cJa is expressed in wing disc clones under the control of Scer\GAL4Scer\FRT.Act5C.

      Expression of ykiS168A.Scer\UAS.T:SV5\V5 suppresses the small eye phenotype seen when brkScer\UAS.cJa is expressed under the control of Scer\GAL4GMR.PF. When tkvQ253D.Scer\UAS.cNc is additionally expressed overgrown eyes are seen.

      Expression of ykiS168A.Scer\UAS.T:SV5\V5 partially suppresses the underproliferation seen when brkScer\UAS.cJa is expressed in wing disc clones under the control of Scer\GAL4Scer\FRT.Act5C. Co-expression of tkvQ253D.Scer\UAS.cNc suppresses the growth defects further, producing some clone overgrowth, whilst the addition of MadScer\UAS.T:Zzzz\FLAG increases clone overgrowth even further.

      Co-expression of MadScer\UAS.T:Zzzz\FLAG and tkvQ253D.Scer\UAS.cNc suppresses the small eye phenotype seen when brkScer\UAS.cJa is expressed under the control of Scer\GAL4GMR.PF.

      (Oh and Irvine, 2011)

      The addition of RbfScer\UAS.cDa to tkvQ253D.Scer\UAS.cNc (driven by Scer\GAL4Act5C.PP) suppresses the proliferation and wing phenotypes seen in tkvQ253D.Scer\UAS.cNc, Scer\GAL4Act5C.PP alone. These animals eclose at normal rates and show at most very mild morphological defects. The addition of Pi3K21BScer\UAS.Δ.T:Ivir\HA1 blocks the growth and proliferation effects tkvQ253D.Scer\UAS.cNc, Scer\GAL4Act5C.PP clones. They contain as few cells as Pi3K21BScer\UAS.Δ.T:Ivir\HA1 (Scer\GAL4Act5C.PP) cells alone, but those cells are slightly larger. When tkvQ253D.Scer\UAS.cNc is driven by Scer\GAL4Act5C.PP in a biomb-3198 or Cdk43 background, no suppression of the proliferation phenotype is seen. When tkvQ253D.Scer\UAS.cNc is driven by Scer\GAL4Act5C.PP in a vg83b27-R background, complete suppression of the proliferation phenotype is seen.

      (Martin-Castellanos and Edgar, 2002)
      Xenogenetic Interactions
      Statement
      Reference

      The presence of BacA\p35Scer\UAS.cHa does not noticeably the increase in cell proliferation seen within or adjacent to somatic clones of tkvQ253D.Scer\UAS.cNc; Scer\GAL4Act5C.Switch.PR cells in the mid third instar wing disc.

      (Rogulja and Irvine, 2005)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Encodes constitutively active form of tkv. Analysed in clones after flipping out FRT cassette from progenitor with Scer\FLP1hs.PG.

      (Burke and Basler, 1996)

      Exists in clones, not reported as being germ-line transmitted.

      (Lecuit and Cohen, 1997, Nellen et al., 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      TKVQ-D
      (Rogulja and Irvine, 2005)
      TKVQ253D
      (Rogulja and Irvine, 2005)
      TKVQD
      (Abu-Shaar and Mann, 1998)
      tkvQ253D.Scer\UAS.cNc
      tkvQ253D.UAS.cNc
      Name Synonyms
      Secondary FlyBase IDs
        References (17)