Allele Dmel\tkv^{Q253D.Scer\UAS.cNb}

General Information
Symbol	Dmel\tkv^{Q253D.Scer\UAS.cNb}	Species	D. melanogaster
Name		FlyBase ID	FBal0050626
Feature type	allele	Associated gene	Dmel\tkv
Also Known As	Tkv^QD

Allele class
Mutagen	in vitro construct - site directed mutagenesis, in vitro construct - amino acid replacement, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Nellen et al., 1996) in vitro construct - regulatory fusion (Yin and Frasch, 1998, Hazelett et al., 1998) in vitro construct - site directed mutagenesis (Nellen et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	tkv^Scer\UAS.cNa (Nellen et al., 1996)
Nature of the lesion	Statement Reference Encodes a constitutively active form of tkv. (Kuhnlein et al., 1997) Construct: Amino acid replacement: Q253D. (Nellen et al., 1996)
Carried in construct	P{UAS-tkv.Q253D.Nb} (Crickmore and Mann, 2007, Marty et al., 2001, Bessa and Casares, 2005, Fujise et al., 2003, Allan et al., 2003, Pena-Rangel et al., 2002, Rangarajan et al., 2001, Nakagoshi et al., 2002, Cavodeassi et al., 2002, de Navas et al., 2006, Gorfinkiel et al., 2003, Hemphala et al., 2003, Kwon et al., 2004, Bennett and Alphey, 2002, Bessa et al., 2002, Bharathi et al., 2004, Moreno and Basler, 2004, Cordero et al., 2007, Tanimoto et al., 2000, Fernandez et al., 2000, Tomoyasu et al., 2000, Steneberg et al., 1999, Fuss and Hoch, 1998, Henderson et al., 1999, Garoia et al., 2005, Kuhnlein et al., 1997, Tsuneizumi et al., 1997, Riesgo-Escovar and Hafen, 1997, Schulz et al., 2004, Brugger et al., 2004, Morel and Arias, 2004, Martin et al., 2004, Keisman and Baker, 2001, Lee and Treisman, 2001, Su et al., 1998, Nellen et al., 1996, Hazelett et al., 1998, Arquier et al., 2001, Shivdasani and Ingham, 2003, Yin and Frasch, 1998, Vincent et al., 1997, Riesgo-Escovar and Hafen, 1997, Glise and Noselli, 1997, Estrada et al., 2006, Crickmore and Mann, 2006, Crickmore and Mann, 2006, Ramel et al., 2007, Letizia et al., 2007, Wada et al., 2007, Rodriguez, 2011, Shen et al., 2008, Zeng et al., 2007, Yan et al., 2009, Schlichting and Dahmann, 2008, Brás-Pereira and Casares, 2008, Fontenele et al., 2009, Barrio et al., 2007, Widmann and Dahmann, 2009, Vuilleumier et al., 2010, Ninov et al., 2010, Higashi-Kovtun et al., 2010, Wang and Ward, 2010, Szuperák et al., 2011, Khuong et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Schulz et al., 2004) visible, with Scer\GAL4^cad-em459 (Gorfinkiel et al., 2003) visible, with Scer\GAL4^dpp.blk1 (Bharathi et al., 2004, Zeng et al., 2007) visible, with Scer\GAL4^GMR.PF (Ramel et al., 2007) visible, with Scer\GAL4^vg.PM (Tsuneizumi et al., 1997)
Phenotype Manifest In
	analia, with Scer\GAL4^cad-em459 (Gorfinkiel et al., 2003) anterior crossvein, with Scer\GAL4^dpp.blk1 (Bharathi et al., 2004) apoptotic amnioserosa (Wada et al., 2007) denticle belt, with Scer\GAL4^69B (Nellen et al., 1996) embryonic/first instar larval cuticle, with Scer\GAL4^69B (Fernandez et al., 2000) embryonic/larval dorsal branch, with Scer\GAL4^btl.PS (Steneberg et al., 1999) embryonic/larval tracheal system, with Scer\GAL4^btl.PS (Vincent et al., 1997) embryonic epidermis, with Scer\GAL4^prd.RG1 (Arquier et al., 2001) epidermal cell \| larval stage \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C} (Ninov et al., 2010) eye, with Scer\GAL4^GMR.PF (Ramel et al., 2007) genitalia, with Scer\GAL4^cad-em459 (Gorfinkiel et al., 2003) germline cell \| male, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Schulz et al., 2004) histoblast \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C} (Ninov et al., 2010) histoblast nest \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C} (Ninov et al., 2010) hub cell, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Schulz et al., 2004) mesoderm, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Yin and Frasch, 1998) ommatidium, with Scer\GAL4^GMR.PF (Ramel et al., 2007) pericardial cell, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Yin and Frasch, 1998) testis \| germline clone, with Scer\GAL4^Act5C.PI (Shivdasani and Ingham, 2003) wing, with Scer\GAL4^nub-AC-62 (Martin et al., 2004, Barrio et al., 2007) wing, with Scer\GAL4^salm-459.2 (Barrio et al., 2007) wing, with Scer\GAL4^vg.PM (Tsuneizumi et al., 1997) wing blade, with Scer\GAL4^dpp.blk1 (Zeng et al., 2007) wing cell, with Scer\GAL4^e22c (Yan et al., 2009) wing cell, with Scer\GAL4^hs.PB (Yan et al., 2009) wing disc, with Scer\GAL4^C-765 (Nellen et al., 1996) wing disc, with Scer\GAL4^nub-AC-62 (Martin et al., 2004) wing disc \| anterior/posterior compartment boundary, with Scer\GAL4^dpp.blk1 (Bharathi et al., 2004) wing margin \| anterior compartment, with Scer\GAL4^vg.int2.1 (Bennett and Alphey, 2002) wing margin bristle \| anterior compartment, with Scer\GAL4^vg.int2.1 (Bennett and Alphey, 2002) wing pouch, with Scer\GAL4^dpp.blk1 (Shen et al., 2008) wing vein, with Scer\GAL4^e22c (Yan et al., 2009) wing vein, with Scer\GAL4^hs.PB (Yan et al., 2009) wing vein, with Scer\GAL4^nub-AC-62 (Barrio et al., 2007) wing vein, with Scer\GAL4^salm-459.2 (Barrio et al., 2007) wing vein L5, with Scer\GAL4^vg.int2.1 (Bennett and Alphey, 2002)
Detailed Description
	Statement Reference Expression oftkv[Q253D.Scer\UAS.cNb] under the control of Scer\GAL4[n-syb.PS] does not result in neuromuscular junction overgrowth in third instar larvae. (Khuong et al., 2010) Histoblasts expressing tkv[Q253D.Scer\UAS.cNb] under the control of Scer\GAL4[Scer\FRT.Act5C] (initiated by heat-shock) show an increased predisposition to associate with the periphery of a histoblast nest edge. Histoblasts undergo dramatic morphogenetic changes and become extremely motile and progressively replace wild-type cells at the periphery to occupy leading positions. No affect on histoblast proliferation is observed. Larval epidermal cells expressing tkv[Q253D.Scer\UAS.cNb] under the control of Scer\GAL4[Scer\FRT.Act5C] (initiated by heat-shock) display slower delamination compared to wild-type larval epidermal cells. (Ninov et al., 2010) tkv[Q253D.Scer\UAS.cNb] expression, driven by Scer\GAL4[e22c] induces ectopic wing vein cells. Expression of tkv[Q253D.Scer\UAS.cNb] after a 20 minute heat-shock at 37[o]C at 18h APF, under the control of Scer\GAL4[hs.PB], results in the formation of ectopic vein cells. (Yan et al., 2009) Anterior overexpression of tkv[Q253D.Scer\UAS.cNb] by Scer\GAL4[dpp.blk1] leads to ectopic apical fold formation along the anteroposterior boundary of the larval wing pouch. In the early pupal stage (5 hours after puparium formation), a small gap develops along the A/P boundary at the distal tip of the wing. By this time, A and P cells are already completely separated and form two smooth apical surfaces. This may lead to the extensive cleft between A and P compartments that forms later in pupal development and which in the adult can lead to a complete separation of anterior and posterior wing blades. In cleft wings caused by posterior bi reduction the posterior wing fragment is characteristically shorter than the anterior fragment. (Shen et al., 2008) Expression of tkv[Q253D.Scer\UAS.cNb] using Scer\GAL4[salm-459.2] modifies wing size, the pattern of veins and the integrity of the wing. Expression of tkv[Q253D.Scer\UAS.cNb] using Scer\GAL4[nub-AC-62] causes the differentiation of most wing cells as vein tissue. (Barrio et al., 2007) Expression of tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^GMR.PF results in large eyes with patterning defects. The ommatidia in these eyes are larger than normal. (Ramel et al., 2007) Expression of tkv^{Q253D.Scer\UAS.cNb} in the peripheral amnioserosa (under the control of Scer\GAL4^pAS) does not affect dorsal closure, which progresses normally with a smooth outer edge of pAS and with internal AS cells being removed over the normal time course. Following completion of dorsal closure, pAS remains associated with the dorsal epidermis, which is subsequently delayed entry into apoptosis. (Wada et al., 2007) Expression of tkv[Q253D.Scer\UAS.cNb] under the control of Scer\GAL4[dpp.blk1] results in a 20.8% penetrant bifurcated wing blade phenotype. (Zeng et al., 2007) Overexpression of tkv^{Q253D.Scer\UAS.cNb} driven by Scer\GAL4^dpp.blk1 in the AP boundary in wild-type wing discs causes mild cleft formation. In adults, defects are seen in the anterior crossvein, otherwise the wing blade is normal. (Bharathi et al., 2004) The wing discs of third instar tkv^{Q253D.Scer\UAS.cNb}; Scer\GAL4^nub-AC-62 larvae have an enlarged wing pouch. The resulting flies have enlarged, poorly patterned wings. (Martin et al., 2004) Expression of tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in accumulation of early germ cell clusters in the testis. Freshly eclosed males show signs of massive cell death in the more distal regions of the testes, while in testes of older males, often only dead or dying tissue is detected. The somatic hub is expanded compared to wild type in larval and adult testes of males expressing tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}. (Schulz et al., 2004) Flies expressing tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^cad-em459 lack both analia and genitalia. (Gorfinkiel et al., 2003) Germ line clones in the testis expressing tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^Act5C.PI overproliferate. (Shivdasani and Ingham, 2003) tkv^{Q253D.Scer\UAS.cNb}; Scer\GAL4^vg.int2.1 flies exhibit distortion, notching and changes in bristle morphology at the anterior wing margin and supression of wing vein L5 formation. (Bennett and Alphey, 2002) Large mutant clones are rarely induced in wing discs. (Nakagoshi et al., 2002) When expression is driven by Scer\GAL4^prd.RG1 embryos are dorsalized. (Arquier et al., 2001) Embryos expressing tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^69B show partial dorsalisation of their cuticles. (Fernandez et al., 2000) Expression driven by Scer\GAL4^btl.PS in a wild type background produces an extra fusion cell per tracheal branch. (Steneberg et al., 1999) Stage 11 embryos expressing tkv^{Q253D.Scer\UAS.cNb} under the control of both Scer\GAL4^how-24B and Scer\GAL4^twi.PG have enlarged clusters of pericardial cell progenitors; each cluster contains approximately 8-10 eve-expressing cells (e-PCs) compared to 3-4 cells per cluster in wild-type embryos. The number of e-PCs decreases to a normal level during early stage 12. Ectopic heart progenitors are produced in the ventrolateral areas of the mesoderm, although the dorsal vessel develops essentially normally without any increase in cell number. (Yin and Frasch, 1998) Scer\GAL4^69B-mediated expression creates dorsalised embryos that undergo normal dorsal closure. Scer\GAL4^69B-mediated expression in Df(2L)flp147E embryos causes a partially dorsalised phenotype and partial rescue of the dorsal closure phenotype. (Riesgo-Escovar and Hafen, 1997) When expression is driven by Scer\GAL4^vg.PM, outgrowth of wing tissue results. (Tsuneizumi et al., 1997) Scer\GAL4^btl.PS-mediated expression causes many cells to move to the dorsal side of the embryo such that dorsal branches of the tracheal system (consisting of 5 to 7 cells in wild type embryos) consist of 25 cells. (Vincent et al., 1997) Expression with Scer\GAL4^69B causes strong dorsalisation of the embryo. Expression with Scer\GAL4^C-765 results in large overproliferating discs. (Nellen et al., 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^dpp.blk1, tkv^{Q253D.Scer\UAS.cNb} has visible phenotype, enhanceable by nmo^P1/nmo[+] (Zeng et al., 2007) tkv^{Q253D.Scer\UAS.cNb} has visible phenotype, enhanceable by coro^ex6/Scer\GAL4^dpp.blk1/coro[+] (Bharathi et al., 2004) tkv^{Q253D.Scer\UAS.cNb} has visible phenotype, enhanceable by Scer\GAL4^dpp.blk1/coro[+]/coro^ex11 (Bharathi et al., 2004)
Suppressed by
Statement Reference Scer\GAL4^dpp.blk1, tkv^{Q253D.Scer\UAS.cNb} has visible phenotype, suppressible \| partially by nmo^Scer\UAS.cUa/Scer\GAL4^dpp.blk1 (Zeng et al., 2007) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, tkv^{Q253D.Scer\UAS.cNb} has visible phenotype, suppressible by Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF/Snoo^EP2510 (Ramel et al., 2007) Scer\GAL4^vg.PM, tkv^{Q253D.Scer\UAS.cNb} has visible phenotype, suppressible by Dad^Scer\UAS.cTa, Scer\GAL4^vg.PM (Tsuneizumi et al., 1997)
Suppressor of
Statement Reference Scer\GAL4^αTub84B.PBb/tkv^{Q253D.Scer\UAS.cNb} is a suppressor of increased cell death \| somatic clone \| cell non-autonomous phenotype of dm^αTub84B.PBb (Moreno and Basler, 2004)
NOT Suppressor of
Statement Reference Scer\GAL4^G14/tkv^{Q253D.Scer\UAS.cNb} is a non-suppressor of neurophysiology defective phenotype of Ranbp11⁷⁰ (Higashi-Kovtun et al., 2010) Scer\GAL4^Rapgap1-OK6/tkv^{Q253D.Scer\UAS.cNb} is a non-suppressor of neurophysiology defective phenotype of Ranbp11⁷⁰ (Higashi-Kovtun et al., 2010)
Other
Statement Reference Scer\GAL4^dpp.blk1, coro^ex11, tkv^{Q253D.Scer\UAS.cNb} has visible phenotype (Bharathi et al., 2004)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^btl.PS, tkv^{Q253D.Scer\UAS.cNb} has embryonic/larval dorsal branch phenotype, enhanceable by N^l1N-ts1 (Steneberg et al., 1999) Scer\GAL4^dpp.blk1, tkv^{Q253D.Scer\UAS.cNb} has wing blade phenotype, enhanceable by nmo^P1/nmo[+] (Zeng et al., 2007) tkv^{Q253D.Scer\UAS.cNb} has wing disc \| anterior/posterior compartment boundary phenotype, enhanceable by coro^ex6/Scer\GAL4^dpp.blk1/coro[+] (Bharathi et al., 2004) tkv^{Q253D.Scer\UAS.cNb} has wing disc \| anterior/posterior compartment boundary phenotype, enhanceable by Scer\GAL4^dpp.blk1/coro[+]/coro^ex11 (Bharathi et al., 2004)
Suppressed by
Statement Reference Scer\GAL4^dpp.blk1, tkv^{Q253D.Scer\UAS.cNb} has wing blade phenotype, suppressible \| partially by nmo^Scer\UAS.cUa/Scer\GAL4^dpp.blk1 (Zeng et al., 2007) Scer\GAL4^e22c, tkv^{Q253D.Scer\UAS.cNb} has wing cell phenotype, suppressible by Egfr^DN.Scer\UAS, Scer\GAL4^e22c (Yan et al., 2009) Scer\GAL4^e22c, tkv^{Q253D.Scer\UAS.cNb} has wing vein phenotype, suppressible by Egfr^DN.Scer\UAS, Scer\GAL4^e22c (Yan et al., 2009) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, tkv^{Q253D.Scer\UAS.cNb} has eye phenotype, suppressible by Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF/Snoo^EP2510 (Ramel et al., 2007) Scer\GAL4^hs.PB, tkv^{Q253D.Scer\UAS.cNb} has wing cell phenotype, suppressible by Egfr^DN.Scer\UAS, Scer\GAL4^hs.PB (Yan et al., 2009) Scer\GAL4^hs.PB, tkv^{Q253D.Scer\UAS.cNb} has wing vein phenotype, suppressible by Egfr^DN.Scer\UAS, Scer\GAL4^hs.PB (Yan et al., 2009) Scer\GAL4^nub-AC-62, tkv^{Q253D.Scer\UAS.cNb} has wing disc phenotype, suppressible by brk^Scer\UAS.cJa/Scer\GAL4^nub-AC-62 (Martin et al., 2004) Scer\GAL4^nub-AC-62, tkv^{Q253D.Scer\UAS.cNb} has wing phenotype, suppressible by brk^Scer\UAS.cJa/Scer\GAL4^nub-AC-62 (Martin et al., 2004) Scer\GAL4^nub-AC-62, tkv^{Q253D.Scer\UAS.cNb} has wing phenotype, suppressible by Snoo^M67/Scer\GAL4^nub-AC-62 (Barrio et al., 2007) Scer\GAL4^nub-AC-62, tkv^{Q253D.Scer\UAS.cNb} has wing vein phenotype, suppressible by Snoo^M67/Scer\GAL4^nub-AC-62 (Barrio et al., 2007) Scer\GAL4^salm-459.2, tkv^{Q253D.Scer\UAS.cNb} has wing phenotype, suppressible by Snoo^M67/Scer\GAL4^salm-459.2 (Barrio et al., 2007) Scer\GAL4^salm-459.2, tkv^{Q253D.Scer\UAS.cNb} has wing vein phenotype, suppressible by Snoo^M67/Scer\GAL4^salm-459.2 (Barrio et al., 2007) Scer\GAL4^vg.PM, tkv^{Q253D.Scer\UAS.cNb} has wing phenotype, suppressible by Dad^Scer\UAS.cTa, Scer\GAL4^vg.PM (Tsuneizumi et al., 1997)
NOT suppressed by
Statement Reference Scer\GAL4^69B, tkv^{Q253D.Scer\UAS.cNb} has embryonic/first instar larval cuticle phenotype, non-suppressible \| germline clone by shark¹ (Fernandez et al., 2000) Scer\GAL4^prd.RG1, tkv^{Q253D.Scer\UAS.cNb} has embryonic epidermis phenotype, non-suppressible by l(2)gl^ts3 (Arquier et al., 2001)
Enhancer of
Statement Reference Scer\GAL4^btl.PS/tkv^{Q253D.Scer\UAS.cNb} is an enhancer of embryonic/larval dorsal branch phenotype of N^l1N-ts1 (Steneberg et al., 1999) Scer\GAL4^n-syb.PS/tkv^{Q253D.Scer\UAS.cNb} is an enhancer of embryonic/larval neuromuscular junction \| third instar larval stage phenotype of nwk¹ (Khuong et al., 2010)
NOT Enhancer of
Statement Reference Scer\GAL4^n-syb.PS/tkv^{Q253D.Scer\UAS.cNb} is a non-enhancer of embryonic/larval neuromuscular junction \| third instar larval stage phenotype of Df(3R)3450/WASp¹ (Khuong et al., 2010)
Suppressor of
Statement Reference Scer\GAL4^e22c/tkv^{Q253D.Scer\UAS.cNb} is a suppressor of FlyBase_internalproperty type:organism:organism \| dorsal closure stage phenotype of Sec61α¹ (Wang and Ward, 2010) Scer\GAL4^Rapgap1-OK6/tkv^{Q253D.Scer\UAS.cNb} is a suppressor of bouton phenotype of Ranbp11⁷⁰ (Higashi-Kovtun et al., 2010) tkv^{Q253D.Scer\UAS.cNb}/Scer\GAL4^69B is a suppressor of embryonic/first instar larval cuticle \| dorsal phenotype of msn¹⁷²/msn¹⁰² (Su et al., 1998) tkv^{Q253D.Scer\UAS.cNb}/Scer\GAL4^332.3 is a suppressor of FlyBase_internalproperty type:organism:organism \| dorsal closure stage phenotype of Sec61α¹ (Wang and Ward, 2010) tkv^{Q253D.Scer\UAS.cNb}/Scer\GAL4^T80 is a suppressor of FlyBase_internalproperty type:organism:organism \| dorsal closure stage phenotype of Sec61α¹ (Wang and Ward, 2010)
NOT Suppressor of
Statement Reference Scer\GAL4^G14/tkv^{Q253D.Scer\UAS.cNb} is a non-suppressor of bouton phenotype of Ranbp11⁷⁰ (Higashi-Kovtun et al., 2010) tkv^{Q253D.Scer\UAS.cNb}/Scer\GAL4^69B is a non-suppressor of dorsal closure embryo \| germline clone phenotype of shark¹ (Fernandez et al., 2000) tkv^{Q253D.Scer\UAS.cNb}/Scer\GAL4^Act5C.PP is a non-suppressor of eye disc phenotype of Scer\GAL4^Act5C.PP, arm^{Δ.Scer\UAS.T:Ivir\HA1} (Hazelett et al., 1998) tkv^{Q253D.Scer\UAS.cNb}/Scer\GAL4^Act5C.PP is a non-suppressor of photoreceptor cell phenotype of Scer\GAL4^Act5C.PP, arm^{Δ.Scer\UAS.T:Ivir\HA1} (Hazelett et al., 1998) tkv^{Q253D.Scer\UAS.cNb}/Scer\GAL4^ey.PH is a non-suppressor of eye disc \| posterior phenotype of Scer\GAL4^ey.PH, wg^Scer\UAS.cAa (Hazelett et al., 1998) tkv^{Q253D.Scer\UAS.cNb}/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor cell phenotype of Scer\GAL4^ey.PH, wg^Scer\UAS.cAa (Hazelett et al., 1998)
Other
Statement Reference Scer\GAL4^dpp.blk1, coro[+]/coro^ex11, tkv^{Q253D.Scer\UAS.cNb} has wing blade \| anterior/posterior compartment boundary phenotype (Bharathi et al., 2004) Scer\GAL4^dpp.blk1, coro^ex6, tkv^{Q253D.Scer\UAS.cNb} has wing blade \| anterior/posterior compartment boundary phenotype (Bharathi et al., 2004) Scer\GAL4^dpp.blk1, coro^ex11, tkv^{Q253D.Scer\UAS.cNb} has wing blade \| anterior/posterior compartment boundary phenotype (Bharathi et al., 2004) Scer\GAL4^ey.PH, arm^{Δ.Scer\UAS.T:Ivir\HA1}, tkv^{Q253D.Scer\UAS.cNb} has eye disc phenotype (Lee and Treisman, 2001, Lee and Treisman, 2001) Scer\GAL4^ey.PH, arm^{Δ.Scer\UAS.T:Ivir\HA1}, tkv^{Q253D.Scer\UAS.cNb} has morphogenetic furrow \| ectopic phenotype (Lee and Treisman, 2001, Lee and Treisman, 2001)
Additional Comments
Genetic Interactions
Statement Reference Expression of tkv[Q253D.Scer\UAS.cNb] under the control of Scer\GAL4[Act.PU] in Df(2L)cbt[S14] clones does not rescue the low cell viability seen in single mutant Df(2L)cbt[S14] clones. (Rodriguez, 2011) Expression of tkv[Q253D.Scer\UAS.cNb] in motor neurons (under the control of Scer\GAL4[OK6]) rescues bouton number and EJP phenotypes in Ranbp11[70] mutants, although mEJP frequency is not rescued. Expression of tkv[Q253D.Scer\UAS.cNb] in muscle (under the control of Scer\GAL4[G14]) does not rescue bouton number or mEJP and EJP phenotypes in Ranbp11[70] mutants. (Higashi-Kovtun et al., 2010) Expression of tkv[Q253D.Scer\UAS.cNb] enhances the neuromuscular junction overgrowth phenotype seen in nwk[1] third instar larvae. (Khuong et al., 2010) Expression of tkv[Q253D.Scer\UAS.cNb] under the control of either Scer\GAL4[e22c], Scer\GAL4[332.3] or Scer\GAL4[T80] results in nearly completely penetrant rescue of the dorsal closure defects of Sec61α[1] homozygous embryos. (Wang and Ward, 2010) Coexpression of tkv[Q253D.Scer\UAS.cNb] with Egfr[DN.Scer\UAS], under the control of Scer\GAL4[e22c], not only fails to induce ectopic vein cells, but also inhibits endogenous vein formation, suggesting that activation of the dpp pathway alone is not sufficient for vein differentiation. Coexpression of tkv[Q253D.Scer\UAS.cNb] with Egfr[DN.Scer\UAS] after a 20 minute heat-shock at 37[o]C at 18h APF, under the control of Scer\GAL4[hs.PB], not only fails to induce ectopic vein cells, but also inhibits endogenous vein formation. (Yan et al., 2009) Co-expression of Snoo[M67] rescues the wing phenotypes caused by Scer\GAL4[salm-459.2]- or Scer\GAL4[nub-AC-62]-mediated expression of tkv[Q253D.Scer\UAS.cNb]. (Barrio et al., 2007) Co-expression of Snoo^EP2510 completely suppresses both the overgrowth and patterning defects seen in the eyes of flies expressing tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^GMR.PF. (Ramel et al., 2007) The bifurcation of the wing caused by expression of tkv[Q253D.Scer\UAS.cNb] under the control of Scer\GAL4[dpp.blk1] is completely suppressed by co-expression of nmo[Scer\UAS.cUa], although wild-type wing morphology is not completely restored. The penetrance of the bifurcated wing phenotype caused by expression of tkv[Q253D.Scer\UAS.cNb] under the control of Scer\GAL4[dpp.blk1] is enhanced by nmo[P1]/+ from 20.8% to 86.3%. (Zeng et al., 2007) Overexpression of tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^dpp.blk1 in coro^ex6 and coro^ex11 heterozygous backgrounds causes a severe cleft phenotype with 85% penetrance along the AP boundary of the wing disc. Enhancement is more pronounced in adult wing blades. (Bharathi et al., 2004) Overexpression of tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^dpp.blk1 in coro^ex6 and coro^ex11 heterozygous backgrounds causes a severe cleft phenotype with 85% penetrance along the AP boundary of the wing disc (as compared to mild cleft formation in tkv^{Q253D.Scer\UAS.cNb} mutants). The enhancement is more pronounced in adult wing blades. (Bharathi et al., 2004) Enlargement of wings in tkv^{Q253D.Scer\UAS.cNb}; Scer\GAL4^nub-AC-62 flies is suppressed by brk^Scer\UAS.cJa. (Martin et al., 2004) Co-expression of arm^{Δ.Scer\UAS.T:Ivir\HA1} and tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^ey.PH in the eye disc often results in ectopic morphogenetic furrow formation from the anterior margin of the disc and a reduction in posterior differentiation. (Lee and Treisman, 2001) The cuticle phenotype of embryos expressing tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^69B is not altered if they are derived from homozygous shark¹ female germ-line clones (and fertilised by a shark⁺ sperm). The dorsal closure defects of homozygous shark¹ embryos derived from shark¹ female germline clones are not rescued by expression of tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^69B. (Fernandez et al., 2000) Expression driven by Scer\GAL4^btl.PS in a N^l1N-ts1 background further increases the number of fusion cells. (Steneberg et al., 1999) Flies expressing wg^Scer\UAS.cAa under the control of Scer\GAL4^ey.PH do not show photoreceptor development at the posterior of the eye disc. This phenotype is not rescued if the flies are also co-expressing tkv^{Q253D.Scer\UAS.cNb}. Clones expressing arm^{Δ.Scer\UAS.T:Ivir\HA1} in the eye disc under the control of Scer\GAL4^Act5C.PP do not show photoreceptor differentiation within the clone. This phenotype is not rescued if the clones are also co-expressing tkv^{Q253D.Scer\UAS.cNb}. eyg¹/Df(3L)iro-2 hemizygous eye discs completely lack photoreceptors. Photoreceptor development in these discs is not rescued by tkv^{Q253D.Scer\UAS.cNb} expressed under the control of Scer\GAL4^dpp.PH. The lack of photoreceptor phenotype of eya¹ eye discs is not rescued by tkv^{Q253D.Scer\UAS.cNb} expressed under the control of Scer\GAL4^ey.PH. (Hazelett et al., 1998) Partially rescues the dorsal open phenotype of msn¹⁰²/msn¹⁷² embryos when expressed under the control of Scer\GAL4^69B. (Su et al., 1998) Dad^Scer\UAS.cTa suppresses the wing outgrowth phenotype of tkv^{Q253D.Scer\UAS.cNb} driven by Scer\GAL4^vg.PM, resulting in an almost wild type wing. (Tsuneizumi et al., 1997)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	36536 w^*; P{UAS-tkv.Q253D.Nb}3/TM3, Sb¹ Ser¹
Notes on Origin
Discoverer

Comments
	Constitutively active. (Nellen et al., 1996)
External Crossreferences & Linkouts
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Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	tkv^{Q253D.Scer\UAS.cNb} tkv^{Q253D.UAS.cNb} tkv^Q253D (Yin and Frasch, 1998) Tkv^QD (Pena-Rangel et al., 2002, Crickmore and Mann, 2006) tkv^QD (Hazelett et al., 1998, Riesgo-Escovar and Hafen, 1997, Shen et al., 2008)
Name Synonym
Secondary FlyBase IDs

References ( 65 )

Generate a list of	All references relating to this allele ( 65 )

List References by type	Research paper ( 62 ) Supplementary material ( 2 ) Review ( 1 )
Recent research papers ( 2 )
	Rodriguez, 2011, PLoS ONE 6(4): e18418 Drosophila TIEG Is a Modulator of Different Signalling Pathways Involved in Wing Patterning and Cell Proliferation. [FBrf0213463] Szuperák et al., 2011, Development 138(4): 715--724 Feedback regulation of Drosophila BMP signaling by the novel extracellular protein Larval Translucida. [FBrf0212881] Show all research papers ...
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	All reviews listed in FlyBase were published before 2011 Show reviews ...

Allele Dmel\tkvQ253D.Scer\UAS.cNb

Allele Dmel\tkv^{Q253D.Scer\UAS.cNb}