Allele Dmel\tkv^Scer\UAS.cNa

General Information
Symbol	Dmel\tkv^Scer\UAS.cNa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Nellen	FlyBase ID	FBal0050627
Feature type	allele	Associated gene	Dmel\tkv

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Nellen et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of a 2.5kb EcoRI-DraI fragment of cDNA STK-A fused to αTub84B transcriptional termination sequences is influences by Scer\UAS Scer\GAL4 binding sites. (Nellen et al., 1996)
Carried in construct	P{UAS-tkv.Na} (Crickmore and Mann, 2007, Myat et al., 2005, Reim and Frasch, 2005, Funakoshi et al., 2001, Tanimoto et al., 2000, Gibson and Perrimon, 2005, Shen and Dahmann, 2005, Kruse et al., 2004, Nellen et al., 1996, Vincent et al., 1997, Crickmore and Mann, 2006, Crickmore and Mann, 2006, Shen et al., 2008, Terriente et al., 2008, Widmann and Dahmann, 2009)
Cytology
Phenotypic Data
Phenotypic Class
	cell shape defective \| third instar larval stage, with Scer\GAL4^ap-md544 (Widmann and Dahmann, 2009) visible, with Scer\GAL4^Act5C.PI (Funakoshi et al., 2001) visible, with Scer\GAL4^dpp.blk1 (Tanimoto et al., 2000)
Phenotype Manifest In
	wing, with Scer\GAL4^dpp.blk1 (Tanimoto et al., 2000) wing, with Scer\GAL4^ptc-559.1 (Shen et al., 2008) wing, with Scer\GAL4^tub.PU (Crickmore and Mann, 2006) wing, with Scer\GAL4^tub.PU, Scer\GAL4^vg.int2.1 (Crickmore and Mann, 2006) wing \| prepupal stage, with Scer\GAL4^ptc-559.1 (Shen et al., 2008) wing disc, with Scer\GAL4^tub.PU (Crickmore and Mann, 2006) wing disc \| third instar larval stage, with Scer\GAL4^ap-md544 (Widmann and Dahmann, 2009) wing pouch, with Scer\GAL4^ptc-559.1 (Shen et al., 2008) wing sensillum, with Scer\GAL4^Act5C.PI (Funakoshi et al., 2001) wing vein, with Scer\GAL4^Act5C.PI (Funakoshi et al., 2001) wing vein L4, with Scer\GAL4^dpp.blk1 (Tanimoto et al., 2000) wing vein L5, with Scer\GAL4^dpp.blk1 (Tanimoto et al., 2000)
Detailed Description
	Statement Reference In early third instar larvae, expression of tkv[Scer\UAS.cNa] in the dorsal wing disc under the control of Scer\GAL4[ap-md544] results in an apical-basal cell length 1.4 times longer than in control cells. Expressing cells are pseudostratified, and the apical cell circumference is decreased compared to control cells. The shape of the prospective dorsal hinge cells are not altered. In late third instar larval wing discs, dorsal pouch cells are moderately longer along their apical-basal axis compared with control cells. (Widmann and Dahmann, 2009) Anterior overexpression of tkv[Scer\UAS.cNa] by Scer\GAL4[ptc-559.1] results in ectopic apical fold formation along the anteroposterior boundary of the larval wing pouch leading to gap formation in the prepupal and adult wing. (Shen et al., 2008) The wings of flies that express tkv^Scer\UAS.cNa under the control of Scer\GAL4^tub are ~30% smaller than wild-type wings. (Crickmore and Mann, 2006) Expression of tkv^Scer\UAS.cNa in the wing and haltere, driven by Scer\GAL4^tub and Scer\GAL4^vg.int2.1, results in wings that are smaller than wild-type flies. Cell size in tkv^Scer\UAS.cNa-overexpressing wings is the same as for wild-type wings. Furthermore, wing disc size is reduced in larvae expressing tkv^Scer\UAS.cNa under the control of only Scer\GAL4^tub. (Crickmore and Mann, 2006) Expression of tkv^Scer\UAS.cNa under the control of Scer\GAL4^Act5C.PI in clones in the wing results in bifurcation of veins and transformation of bristles. (Funakoshi et al., 2001) Expression of tkv^Scer\UAS.cNa under the control of Scer\GAL4^dpp.blk1 results in small wings with abnormal patterning in the central region of the wing. Veins 4 and 5 appear to partially fuse and there is abnormal overgrowth of the region between veins 3 and 4. (Tanimoto et al., 2000) Coexpression of P{UAS-tkv} and P{UAS-put.N} with Scer\GAL4^69B causes strong dorsalisation of the embryo. Such embryos fail to develop ventral denticle belts and exhibit dorsal hairs along the entire dorsoventral axis. Expression of P{UAS-tkv} alone with Scer\GAL4^69B has no effect on embryonic patterning. (Nellen et al., 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^tub.PU, tkv^Scer\UAS.cNa has wing phenotype, enhanceable by dpp^hr4/dpp^d6 (Crickmore and Mann, 2006)
Enhancer of
Statement Reference tkv^Scer\UAS.cNa/Scer\GAL4^tub.PU is an enhancer of wing phenotype of dpp^hr4/dpp^d6 (Crickmore and Mann, 2006)
Suppressor of
Statement Reference Scer\GAL4^Act5C.PU/tkv^Scer\UAS.cNa is a suppressor \| partially of haltere disc \| posterior compartment phenotype of Ubx^pbx-1/Ubx^9.22 (Crickmore and Mann, 2006)
Additional Comments
Genetic Interactions
Statement Reference Expression of tkv^Scer\UAS.cNa under the control of Scer\GAL4^Act5C in a Ubx^pbx-1/Ubx^9.22 background reduces the size of the P compartment of haltere discs relative to that observed in Ubx^pbx-1/Ubx^9.22 discs (from 1.45 to 0.83), although this ratio is still greater than that of wild-type discs. (Crickmore and Mann, 2006) Flies that express tkv^Scer\UAS.cNa, under the control of Scer\GAL4^tub, in a dpp^hr4/dpp^d6 background, have smaller wings than flies expressing the transgene in a wild-type background and than dpp^hr4/dpp^d6 flies that do not express tkv^Scer\UAS.cNa. (Crickmore and Mann, 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	tkv^Scer\UAS.cNa/Scer\GAL4^tub.PU rescues tkv^xtr (Gibson and Perrimon, 2005)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	tkv^Scer\UAS.cNa tkv^UAS.cNa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Nellen (Nellen et al., 1996)
Secondary FlyBase IDs

References ( 15 )
Research paper	Widmann and Dahmann, 2009, J. Cell Sci. 122(9): 1362--1373 Dpp signaling promotes the cuboidal-to-columnar shape transition of Drosophila wing disc epithelia by regulating Rho1. [FBrf0207672] Shen et al., 2008, Mech. Dev. 125(3-4): 233--246 optomotor-blind suppresses instability at the A/P compartment boundary of the Drosophila wing. [FBrf0202782] Terriente et al., 2008, Dev. Biol. 320(1): 102--112 The Drosophila gene zfh2 is required to establish proximal-distal domains in the wing disc. [FBrf0207390] Crickmore and Mann, 2007, Development 134(2): 327--334 Hox control of morphogen mobility and organ development through regulation of glypican expression. [FBrf0194329] Crickmore and Mann, 2006, Science 313(5783): 63--68 Hox control of organ size by regulation of morphogen production and mobility. [FBrf0195181] Gibson and Perrimon, 2005, Science 307(5716): 1785--1789 Extrusion and death of DPP/BMP-compromised epithelial cells in the developing Drosophila wing. [FBrf0184250] Myat et al., 2005, Dev. Biol. 281(1): 38--52 A molecular link between FGF and Dpp signaling in branch-specific migration of the Drosophila trachea. [FBrf0187390] Reim and Frasch, 2005, Development 132(22): 4911--4925 The Dorsocross T-box genes are key components of the regulatory network controlling early cardiogenesis in Drosophila. [FBrf0190316] Shen and Dahmann, 2005, Dev. Biol. 279(1): 31--43 The role of Dpp signaling in maintaining the Drosophila anteroposterior compartment boundary. [FBrf0183859] Kruse et al., 2004, Development 131(19): 4843--4856 Dpp gradient formation by dynamin-dependent endocytosis: receptor trafficking and the diffusion model. [FBrf0180152] Funakoshi et al., 2001, Development 128(1): 67--74 mtv shapes the activity gradient of the Dpp morphogen through regulation of thickveins. [FBrf0131291] Tanimoto et al., 2000, Mol. Cell 5(1): 59--71 Hedgehog creates a gradient of DPP activity in Drosophila wing imaginal discs. [FBrf0125394] Vincent et al., 1997, Development 124(14): 2741--2750 DPP controls tracheal cell migration along the dorsoventral body axis of the Drosophila embryo. [FBrf0097693] Nellen et al., 1996, Cell 85(3): 357--368 Direct and long-range action of a DPP morphogen gradient. [FBrf0087626]
Supplementary material	Crickmore and Mann, 2006, Science 3485(5783): Supporting online material. [FBrf0199087]

Allele Dmel\tkvScer\UAS.cNa

Allele Dmel\tkv^Scer\UAS.cNa