Allele Dmel\trpl³⁰²

General Information
Symbol	Dmel\trpl³⁰²	Species	D. melanogaster
Name		FlyBase ID	FBal0050670
Feature type	allele	Associated gene	Dmel\trpl
Also Known As	trpl^p302

Map ( GBrowse )
Allele class	amorphic allele - genetic evidence, loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	amorphic allele - genetic evidence (Niemeyer et al., 1996) loss of function allele (Reuss et al., 1997)
Mutagen	ethyl methanesulfonate (Niemeyer et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:5,649,467..5,649,467 comment=site of nucleic acid difference inferred by FlyBase curator based on reported amino acid change evidence=experimental pr_change=Q302@ na_change=C5649467T (Niemeyer et al., 1996)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: ?302@. Amber nonsense codon leading to premature termination of the polypeptide chain before the first transmembrane segment. (Niemeyer et al., 1996)
Cytology
Phenotypic Data
Phenotypic Class
	auditory perception defective \| adult stage (Senthilan et al., 2012) neurophysiology defective (Reuss et al., 1997, Leung et al., 2000) neurophysiology defective \| adult stage (Senthilan et al., 2012) thermotaxis behavior defective \| third instar larval stage (Kwon et al., 2010) thermotaxis behavior defective \| third instar larval stage (with trpl^MB03075) (Kwon et al., 2010) visual behavior defective (Li et al., 1999, Baehner et al., 2002, Reuss et al., 1997, Chyb et al., 1999, Leung et al., 2000)
Phenotype Manifest In
	Malpighian tubule (Macpherson et al., 2005) photoreceptor (Reuss et al., 1997) photoreceptor cell (Li et al., 1999, Chyb et al., 1999, Leung et al., 2000, Haab et al., 2000) photoreceptor cell & axon (Niemeyer et al., 1996)
Detailed Description
	Statement Reference Compound action potentials can be evoked by sound in the antennal nerve of mutant flies, but the sound particle velocities required to elicit the response is increased compared to wild type. Nonlinear mechanical amplification is significantly reduced compared to wild type. (Senthilan et al., 2012) trpl[302] mutants show a sustained response to prolonged light. (Richter et al., 2011) Mutant flies exhibit the wild type avoidance of aristolochic acid in food choice assays. (Kim et al., 2010) The normal preference of third instar larvae for 17.5[o]C over 14[o]C in a two-way choice test is eliminated in trpl[302] and trpl[302]/trpl[MB03075] larvae. (Kwon et al., 2010) trpl[302] mutants retain a preference for 18[o]C (i.e. are thermotactic). (Kwon et al., 2008) capa stimulated fluid transport rates are significantly reduced in Malpighian tubules from trpl³⁰² animals compared to control tubules, while the basal rate of secretion is unaffected. (Macpherson et al., 2005) Mutant flies show an electroretinogram response that is similar to wild-type in response to a 10 second pulse of light. (Wang et al., 2005) The reduction in electroretinogram (ERG) amplitude seen in flies exposed to constant light is not suppressed by trpl³⁰². (Lee and Montell, 2004) Wild-type flies kept in the dark are significantly more sensitive to background light than wild-type flies kept in the light or trpl³⁰² flies kept in light or darkness. (Baehner et al., 2002) Mutant phenotype can be detected using ERG and intracellular recording techniques after prolonged stimuli. The receptor potential has a smaller sustained component than wild type, with altered time course of decay, though peak responses are similar. The response is accompanied by oscillations during stimulus and hyperpolarization after stimulus termination. Mutants also show inability to adapt to dim background illuminations. Refractory period and response latency are wild type. (Leung et al., 2000) When IBMX is applied to trpl³⁰² mutant flies the macroscopic response is slowed in a way that is no different to that of wild type flies exposed to IBMX. (Chyb et al., 1999) E_rev in mutant photoreceptors shows a small but statistically highly significant positive shift compared to wild type. Light sensitive conductance is even more permeable to Na⁺ than wild type. Permeability to Li⁺ is roughly the same in wild type, trpl³⁰² and trp² mutants. In general the permeability of mutant photoreceptors to Ca²⁺, Na⁺, Li⁺, Ba²⁺, Mg²⁺ and Mn²⁺ is higher than that for wild type. The I-V relationship of the light sensitive conductance is similar to that of wild type in always showing a pronounced dual rectification. Shows no biphasic reversal potential behaviour with lowered external calcium. Estimated single channel conductance is 4pS. La³⁺ completely blocks the light response. Light sensitive conductance is facilitated by Ca²⁺. (Reuss et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference trpl³⁰² has visual behavior defective phenotype, enhanceable by inaF-D^P106x (Li et al., 1999) trpl³⁰² has visual behavior defective phenotype, enhanceable by trp² (Li et al., 1999) trpl³⁰² has visual behavior defective phenotype, enhanceable by trp⁹ (Li et al., 1999)
Enhancer of
Statement Reference trpl³⁰² is an enhancer of neurophysiology defective phenotype of Xport¹ (Rosenbaum et al., 2011)
Other
Statement Reference inaD^P215, trpl³⁰² has visual behavior defective phenotype (Leung et al., 2000, Leung et al., 2000) trp¹, trpl³⁰² has visual behavior defective phenotype (Reuss et al., 1997, Reuss et al., 1997) trp², trpl³⁰² has visual behavior defective phenotype (Leung et al., 2000, Leung et al., 2000, Reuss et al., 1997, Reuss et al., 1997) trp⁹, trpl³⁰² has circadian rhythm defective \| recessive phenotype (Yang et al., 1998, Yang et al., 1998) trp⁹, trpl³⁰² has neurophysiology defective phenotype (Huang et al., 2004, Krause et al., 2008)
Phenotype Manifest In
Enhanced by
Statement Reference trpl³⁰² has photoreceptor cell phenotype, enhanceable by inaF-D^P106x (Li et al., 1999) trpl³⁰² has photoreceptor cell phenotype, enhanceable by trp² (Li et al., 1999) trpl³⁰² has photoreceptor cell phenotype, enhanceable by trp⁹ (Li et al., 1999)
Suppressor of
Statement Reference trp¹/trpl³⁰² is a suppressor of ommatidium & microvillus phenotype of rdgA¹ (Raghu et al., 2000) trp¹/trpl³⁰² is a suppressor of ommatidium phenotype of rdgA¹ (Raghu et al., 2000) trp²/trpl³⁰² is a suppressor of ommatidium & microvillus phenotype of rdgA¹ (Raghu et al., 2000) trp²/trpl³⁰² is a suppressor of ommatidium phenotype of rdgA¹ (Raghu et al., 2000) trpl³⁰²/trp⁹ is a suppressor of ommatidium & microvillus phenotype of rdgA¹ (Raghu et al., 2000) trpl³⁰²/trp⁹ is a suppressor of ommatidium phenotype of rdgA¹ (Raghu et al., 2000)
NOT Suppressor of
Statement Reference trpl³⁰² is a non-suppressor of phenotype of rdgA¹ (Raghu et al., 2000)
Other
Statement Reference inaD^P215, trpl³⁰² has photoreceptor cell phenotype (Leung et al., 2000, Leung et al., 2000) trp¹, trpl³⁰² has photoreceptor cell phenotype (Chyb et al., 1999, Chyb et al., 1999) trp¹, trpl³⁰² has photoreceptor phenotype (Reuss et al., 1997, Reuss et al., 1997) trp², trpl³⁰² has photoreceptor cell & axon phenotype (Niemeyer et al., 1996, Niemeyer et al., 1996) trp², trpl³⁰² has photoreceptor cell phenotype (Leung et al., 2000, Leung et al., 2000) trp², trpl³⁰² has photoreceptor phenotype (Reuss et al., 1997, Reuss et al., 1997) trp⁹, trpl³⁰² has ommatidium phenotype (Krause et al., 2008) trp⁹, trpl³⁰² has photoreceptor cell phenotype (Haab et al., 2000, Agam et al., 2000, Haab et al., 2000, Agam et al., 2000) trp⁹, trpl³⁰² has retina phenotype (Huang et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference The trpl[302]; trp[9] double mutant has no functional trp and trpl channels and shows no response to light. (Richter et al., 2011) Dissociated Xport[1], trpl[302] double mutant ommatidia show a residual response to brief light flashes; sensitivity is reduced ~500-fold with respect to wild type and 25-fold with respect to Xport[1]. (Rosenbaum et al., 2011) Illumination of dissociated, patch-clamped ommatidia from trp[9];trpl[302] double mutants show activation of the whole phototransduction cascade, do not show any light-induced current or Ca[2+] influx, but do show a normal light-dependent modulation of the delayed rectifier current mediated by Shab channels. Increasing the light level by a factor of ten results in an upregulation of approximately 30% and recovery to baseline within 30 minutes, similar to wildtype controls. (Krause et al., 2008) trp⁹; trpl³⁰² individual photoreceptors form the correct number of synapses per presynaptic terminal independently of cartridge composition. The photoreceptor terminals of these mutants show an increase in glial invaginations. (Hiesinger et al., 2006) capa stimulated fluid transport rates are significantly reduced in Malpighian tubules from trpl³⁰² trp⁹ animals compared to control tubules, while the basal rate of secretion is unaffected. (Macpherson et al., 2005) trpl³⁰² ; trp¹⁴ flies show a electroretinogram response in response to a 10 second pulse of light that is similar to that seen in trp¹⁴ single mutants. trpl³⁰² ; trp^L612F.ninaE trp⁹ flies have a wild-type electroretinogram response. trpl³⁰² ; trp^ninaE.PW trp⁹ flies have a wild-type electroretinogram response. trpl³⁰² ; trp^R671Q.ninaE trp⁹ flies have a transient electroretinogram response to a 10 second pulse of light. (Wang et al., 2005) trp⁹ ; trpl³⁰² double mutants show a reduced depolarisation response to anoxia in the photoreceptors compared to wild-type flies; the amplitude of anoxia-induced depolarisation in the double mutants is half that of wild type and the response kinetics are much slower than normal. (Huang et al., 2004) In trp⁹,trpl³⁰² double mutants the second, large phase depolarization response to anoxia (caused by N₂ and measured in the eye by extracellular voltage change recordings), fails, indicating that the small but not the large phase of the response resulted from accumulation of K⁺ in the extracellular space. In trp⁹, trpl³⁰² double mutants the Ca²⁺ response to anoxia is virtually abolished. (Agam et al., 2000) Excised patches from rhabdomeral membranes show no sustained spontaneous activity nor high frequency burst-like behavior in trp⁹,trpl³⁰² double mutants. (Haab et al., 2000) Rhabdomeres of trpl³⁰²,inaD¹ double mutants are intact in 1 day old flies. ERG light responses of trpl³⁰²,inaD¹ and trpl³⁰²,trp² are small and transient, but the response amplitude of trpl³⁰²,inaD¹ is significantly smaller than that of trpl³⁰²,trp². Similarly, refractory period and response latency defects are more severe for trpl³⁰²,inaD¹ than for trpl³⁰²,trp². (Leung et al., 2000) In trp¹, trpl³⁰² double mutants light sensitive channels are not activated by the application of linolenic acid, whereas they are activated in single mutant channels. (Chyb et al., 1999) Double mutant analysis comparing the receptor potentials of trpl³⁰² with inaF^P106x; trpl³⁰² or trp²; trpl³⁰² or trp⁹; trpl³⁰² showed that the effects of inaF^P106x are as severe as those of trp² and are specific to the trp channel. (Li et al., 1999) Behavioural rhythms of trp⁹ trpl³⁰² double mutants show reduced resetting in response to a pulse of light. (Yang et al., 1998) trpl³⁰²; trp² double mutants are almost blind as judged by response to saturating ultraviolet flashes and ERG recordings. trpl³⁰²; trp¹ double mutants are blind. (Reuss et al., 1997) Whole-cell patch-clamp recordings of photoreceptors display sensitivity and response kinetics indistinguishable from wild type. trpl³⁰²; trp² mutants display a dramatic loss of responsiveness, the residual current is due to a small amount of functional trp channel. trpl³⁰²; trp² mutants exhibit a dramatic bump phenotype. (Niemeyer et al., 1996)
Xenogenetic Interactions
Statement Reference The presence of trp::trpl[ninaE.trp1-675.trpl681-1124.T:Avic\GFP-EGFP] in trpl[302]; trp[9] double mutants results in a response to light that declines towards baseline during prolonged intense light. The presence of trp::trpl[ninaE.trpl1-336.T:Avic\GFP-EGFP] in trpl[302]; trp[9] double mutants results in a response to light that declines towards baseline during prolonged intense light. The presence of trp::trpl[ninaE.trpl1-336.trp328-675.trpl681-1124.T:Avic\GFP-EGFP] in trpl[302]; trp[9] double mutants results in a response to light that declines towards baseline during prolonged intense light. The presence of trp::trpl[ninaE.trp1-328.trpl336-681.trp675-1275.T:Avic\GFP-EGFP] in trpl[302]; trp[9] double mutants results in a response to light that declines towards baseline during prolonged intense light. (Richter et al., 2011)
Complementation & Rescue Data
Rescued by	trpl³⁰² is rescued by trpl^H678R.ninaE (Wang et al., 2005) trpl³⁰² is rescued by trpl^hs.PN (Macpherson et al., 2005, Niemeyer et al., 1996) trpl³⁰² is rescued by trpl^ninaE.PW (Wang et al., 2005)
Comments	Reintroduction of trpl^hs.PN to trpl³⁰²; trp² mutants rescues the defect and restores visual physiology. Photoreceptor cells produce quantum bumps (the unitary event of a single photon) that are indistinguishable from wild type. (Niemeyer et al., 1996)
Stocks ( 1 )
Bloomington	31433 cn¹ trpl³⁰² bw¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	trpl³⁰² (Hiesinger et al., 2006, Satoh et al., 2010, Huang et al., 2004, Wang et al., 2005, Meyer et al., 2006, Vasiliauskas et al., 2011, Rosenbaum et al., 2006, Krause et al., 2008, Chen et al., 2010, Kwon et al., 2010, Kim et al., 2010, Rosenbaum et al., 2011, Elsaesser et al., 2010, Senthilan et al., 2012, Richter et al., 2011) trpl^p302 (Haab et al., 2000) trpl^P302 (Kwon et al., 2008)
Name Synonym
Secondary FlyBase IDs

References ( 33 )

Generate a list of	All references relating to this allele ( 33 )

List References by type	Research paper ( 33 )
Recent research papers ( 4 )
	Senthilan et al., 2012, Cell 150(5): 1042--1054 Drosophila auditory organ genes and genetic hearing defects. [FBrf0219321] Richter et al., 2011, J. Biol. Chem. 286(39): 34234--34243 Translocation of the Drosophila Transient Receptor Potential-like (TRPL) Channel Requires Both the N- and C-terminal Regions Together with Sustained Ca2+ Entry. [FBrf0216251] Rosenbaum et al., 2011, Neuron 72(4): 602--615 XPORT-Dependent Transport of TRP and Rhodopsin. [FBrf0216705] Vasiliauskas et al., 2011, Nature 479(7371): 108--112 Feedback from rhodopsin controls rhodopsin exclusion in Drosophila photoreceptors. [FBrf0216554] Show all research papers ...

Allele Dmel\trpl302

Allele Dmel\trpl³⁰²