FlyBase Allele Report: Dmel\twi[UAS.cBa]

General Information

Symbol

Dmel\twi^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Baylies

FlyBase ID

FBal0050680

Feature type

allele

Associated gene

Dmel\twi

Associated Insertion(s)

Carried in Construct

P{UAS-twi.B}

Also Known As

UAS-twi, UAS-twist

Key Links

Transgenic product class

cDNA

(Baylies and Bate, 1996)

wild_type

(Baylies and Bate, 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Baylies and Bate, 1996)

wild_type

(Baylies and Bate, 1996)

Mutagen

in vitro construct

(Anant et al., 1998, Gajewski et al., 1998, Baylies and Bate, 1996, Taylor et al., 1995)

Progenitor genotype

Carried in construct

P{UAS-twi.B}

Cytology

Description

Expression of a full length twi cDNA is governed by UASt regulatory sequences.

(Baylies and Bate, 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

twi

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal lateral oblique muscle 1 founder cell, with Scer\GAL4^twi.PB

(Cox and Baylies, 2005)

abdominal ventral transverse muscle 1 founder cell, with Scer\GAL4^twi.PB

(Cox and Baylies, 2005)

corpus cardiacum primordium, with Scer\GAL4^twi.2PE

(Park et al., 2011)

dorsal vessel wall cell, with Scer\GAL4^twi.PB

(Baylies and Bate, 1996)

embryonic/larval dorsal vessel | embryonic stage, with Scer\GAL4^twi.PB

(Baylies and Bate, 1996)

embryonic/larval heart, with Scer\GAL4^twi.PB

(Castanon et al., 2001)

embryonic epidermis, with Scer\GAL4^da.G32

(Baylies and Bate, 1996)

embryonic somatic muscle cell | ectopic, with Scer\GAL4^twi.PB

(Castanon et al., 2001)

indirect flight muscle cell, with Scer\GAL4¹¹⁵¹

(Bernard et al., 2009, Anant et al., 1998)

larval pericardial cell, with Scer\GAL4^twi.PB

(Baylies and Bate, 1996)

mesoderm, with Scer\GAL4^twi.PG

(Halfon et al., 2000)

myoblast | P-stage, with Scer\GAL4¹¹⁵¹

(Dutta et al., 2004)

visceral muscle cell, with Scer\GAL4^twi.PB

(Baylies and Bate, 1996)

visceral trunk mesoderm, with Scer\GAL4^twi.PB

(Castanon et al., 2001, Baylies and Bate, 1996)

Detailed Description

Statement

Reference

The gl-positive corpora cardiaca precursor cells are missing in stage 12 embryos expressing twi^Scer\UAS.cBa under the control of Scer\GAL4^twi.2PE.

(Park et al., 2011)

Developing indirect flight muscles degenerate in animals expressing twi^Scer\UAS.cBa under the control of Scer\GAL4¹¹⁵¹.

(Bernard et al., 2009)

When twi^Scer\UAS.cBa is driven by Scer\GAL4^twi.PB mutant embryos exhibit a near complete loss of cluster I slou expressing muscle founder cells. Cluster II cells are unaffected.

(Cox and Baylies, 2005)

There are no dorsal-ventral indirect flight muscle founder myoblasts in twi^Scer\UAS.cBa; Scer\GAL4¹¹⁵¹ pupae at 12 hours after puparium formation.

(Dutta et al., 2004)

Over expression of twi^Scer\UAS.cBa when driven by Scer\GAL4^twi.PB converts non-somatic mesoderm into somatic muscle. Ectopic multinucleated somatic muscle cells are detected dorsally where the heart normally forms and around the gut and central nervous system. These muscles do not spread out and form a pattern owing to lack of epidermis.

(Castanon et al., 2001)

Expression of twi^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG leads to occasional extra eve-expressing progenitor cells in the mesoderm.

(Halfon et al., 2000)

The larval templates of the indirect flight muscles do not split in animals expressing twi^Scer\UAS.cBa under the control of Scer\GAL4¹¹⁵¹ and the indirect flight muscles degenerate completely. Direct flight muscles are present and appear normal.

(Anant et al., 1998)

When expression is driven by Scer\GAL4^how-24B somatic myogenesis and segregation of twi expressing adult myoblasts occur normally. With extra copies of P{UAS-twi.B} some somatic muscle patterning defects do occur and minor defects in the differentiation of visceral mesoderm (e.g. gastric caecae) and heart occur. When expression is driven by Scer\GAL4^twi.PB, the somatic muscles develop normally but the development of the visceral mesoderm and heart are disturbed. The number of visceral mesoderm progenitors was reduced and the cardial and pericardial cells were missing or deranged. Somatic muscle-like structures form on the gut and heart. When expression is driven by Scer\GAL4^da.G32 ectodermal derivatives fail to develop. External (normally epidermal) cells express myosin and fuse to form bi- or tri-nucleate syncytia.

(Baylies and Bate, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^twi.PB, twi^UAS.cBa has embryonic somatic muscle cell | ectopic phenotype, enhanceable by da⁵

(Castanon et al., 2001)

Scer\GAL4^twi.PB, twi^UAS.cBa has embryonic/larval heart phenotype, enhanceable by da⁵

(Castanon et al., 2001)

Scer\GAL4^twi.PB, twi^UAS.cBa has visceral trunk mesoderm phenotype, enhanceable by da⁵

(Castanon et al., 2001)

Scer\GAL4^twi.PG, twi^UAS.cBa has mesoderm phenotype, enhanceable by Ras85D^G13Q.UAS, Scer\GAL4^twi.PG

(Halfon et al., 2000)

Scer\GAL4^twi.PG, twi^UAS.cBa has mesoderm phenotype, enhanceable by wg^UAS.cGa/Ras85D^G13Q.UAS, Scer\GAL4^twi.PG

(Halfon et al., 2000)

Scer\GAL4^twi.PG, twi^UAS.cBa has mesoderm phenotype, enhanceable by Scer\GAL4^twi.PG/wg^UAS.cGa

(Halfon et al., 2000)

Suppressed by

Statement

Reference

twi^UAS.cBa has abdominal ventral transverse muscle 1 founder cell phenotype, suppressible by Scer\GAL4^twi.PB/wg^l-17

(Cox and Baylies, 2005)

Enhancer of

Statement

Reference

twi^UAS.cBa, Scer\GAL4^twi.PG is an enhancer of mesoderm phenotype of Ras85D^G13Q.UAS, Scer\GAL4^twi.PG

(Halfon et al., 2000)

wg^UAS.cGa/twi^UAS.cBa, Scer\GAL4^twi.PG is an enhancer of mesoderm phenotype of Ras85D^G13Q.UAS, Scer\GAL4^twi.PG

(Halfon et al., 2000)

Suppressor of

Statement

Reference

Scer\GAL4^twi.PB/twi^UAS.cBa is a suppressor of abdominal lateral oblique muscle 1 founder cell phenotype of wg^l-17

(Cox and Baylies, 2005)

Scer\GAL4^twi.PB/twi^UAS.cBa is a suppressor of abdominal ventral transverse muscle 1 founder cell phenotype of wg^l-17

(Cox and Baylies, 2005)

NOT Suppressor of

Statement

Reference

Scer\GAL4^twi.PB/twi^UAS.cBa is a non-suppressor of abdominal ventral acute muscle 1 founder cell phenotype of wg^l-17

(Cox and Baylies, 2005)

Scer\GAL4^twi.PB/twi^UAS.cBa is a non-suppressor of abdominal ventral acute muscle 2 founder cell phenotype of wg^l-17

(Cox and Baylies, 2005)

Scer\GAL4^twi.PB/twi^UAS.cBa is a non-suppressor of abdominal ventral acute muscle 3 founder cell phenotype of wg^l-17

(Cox and Baylies, 2005)

Other

Statement

Reference

Scer\GAL4^twi.PB, twi^UAS.cBa, wg^l-17 has abdominal lateral oblique muscle 1 founder cell phenotype

(Cox and Baylies, 2005)

Scer\GAL4^twi.PB, twi^UAS.cBa, wg^l-17 has abdominal ventral acute muscle 1 founder cell phenotype

(Cox and Baylies, 2005)

Scer\GAL4^twi.PB, twi^UAS.cBa, wg^l-17 has abdominal ventral acute muscle 2 founder cell phenotype

(Cox and Baylies, 2005)

Scer\GAL4^twi.PB, twi^UAS.cBa, wg^l-17 has abdominal ventral acute muscle 3 founder cell phenotype

(Cox and Baylies, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The number of extra eve-expressing progenitor cells in the mesoderm is substantially increased when twi^Scer\UAS.cBa is co-expressed with wg^Scer\UAS.cGa under the control of Scer\GAL4^twi.PG. Co-expression of twi^Scer\UAS.cBa and Ras85D^{G13Q.Scer\UAS} under the control of Scer\GAL4^twi.PG results in a lateral expansion of the eve-expressing progenitor clusters in the mesoderm. Co-expression of twi^Scer\UAS.cBa, Ras85D^{G13Q.Scer\UAS} and wg^Scer\UAS.cGa under the control of Scer\GAL4^twi.PG results in an almost continuous anteroposterior stripe of eve-expressing progenitor cells confined to the dorsal mesoderm.

(Halfon et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^twi.PB/twi^UAS.cBa rescues twi¹

(Castanon et al., 2001)

Comments

twi^Scer\UAS.cBa completely rescues all phenotypes seen in twi¹/twi¹. The addition of da⁵/+ to twi^Scer\UAS.cBa, Scer\GAL4^twi.PB embryos enhances the losses in gut and heart forming mesoderm. Two copies can also enhance the ectopic muscle phenotype as well.

(Castanon et al., 2001)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

twi^Scer\UAS.cBa

twi^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Baylies

(Baylies and Bate, 1996)

Secondary FlyBase IDs

References (22)

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