FlyBase Allele Report: Dmel\ttk[rM730]

General Information

Symbol

Dmel\ttk^rM730

Species

D. melanogaster

Name

FlyBase ID

FBal0051010

Feature type

allele

Associated gene

Dmel\ttk

Associated Insertion(s)

P{PZ}ttk^rM730

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Lai and Li, 1999, Giesen et al., 1997)

Mutagen

P-element activity

(Giesen et al., 1997, Lai et al., 1996)

Progenitor genotype

Associated Insertion(s)

P{PZ}ttk^rM730

Cytology

Description

P-element insertion 1.2kb upstream of the first exon.

(Giesen et al., 1997)

P-element insertion within enhancer elements of ttk.

(Lai et al., 1996)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Murawsky et al., 2001)

lethal - all die during embryonic stage | recessive

(Lai et al., 1996)

some die during embryonic stage | recessive

(Lai et al., 1996)

visible | somatic clone

(Siddall et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

ttk^rM730 mutant eye clones display severe degeneration of the corneal lens, with ommatidia and sensory bristles failing to properly develop.

In 90% of ttk^rM730 mutant third instar eye disc clones, 2-3 presumptive R7 photoreceptor cells are observed seven rows posterior to the morphogenetic furrow, compared to just one R7 cell in control tissue. A reduced number of cone cells is observed in mutant clones.

(Siddall et al., 2009)

The number of neurons associated with the pentascolopidial sensory organ is approximately double that of wild type in mutant embryos.

(Murawsky et al., 2001)

Homozygous clones in the adult eye cause degeneration of the corneal lens and a failure of photoreceptor development. Rhabdomeres of photoreceptors are not observed in clones, but residual cellular structures in the mutant ommatidia are still recognizable. Genetically mosaic ommatidia are not seen near the boundary of the clone. Ectopic neurons are not seen between or below the developing ommatidia in homozygous clones in third instar larvae. Larval eye development seems close to normal in the absence of ttk, although the ommatidial clusters in clones were somewhat disorganised. Cone cell development appears normal.

(Lai and Li, 1999)

Large mitotic clones in the eye were rarely observed, small clones often causes scars.

(Lai et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

HDAC1^1-3, ttk^rM730 has visible phenotype, enhanceable by Mi-2⁴/Mi-2[+]

(Murawsky et al., 2001)

HDAC1^3-10, ttk^rM730 has visible phenotype, enhanceable by Mi-2⁴/Mi-2[+]

(Murawsky et al., 2001)

HDAC1^5-5, ttk^rM730 has visible phenotype, enhanceable by Mi-2⁴/Mi-2[+]

(Murawsky et al., 2001)

ttk^rM730 has abnormal neuroanatomy phenotype, enhanceable by Mi-2⁴

(Murawsky et al., 2001)

Suppressed by

Statement

Reference

ttk^rM730 has visible | somatic clone phenotype, suppressible by lz^mr2

(Siddall et al., 2009)

NOT Suppressor of

Statement

Reference

ttk^rM730 is a non-suppressor of increased mortality during development phenotype of Scer\GAL4^nanos.PG, run^UAS.cLa

(Vander Zwan et al., 2003)

ttk[+]/ttk^rM730 is a non-suppressor of partially lethal phenotype of Scer\GAL4^nanos.PG, run^UAS.cLa

(Wheeler et al., 2002)

Other

Statement

Reference

HDAC1^5-5, ttk^rM730 has visible phenotype

(Murawsky et al., 2001)

HDAC1^1-3, ttk^rM730 has visible phenotype

(Murawsky et al., 2001)

HDAC1^3-10, ttk^rM730 has visible phenotype

(Murawsky et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

ttk^rM730 has larval abdominal lch5 neuron | increased number phenotype, enhanceable by Mi-2⁴

(Murawsky et al., 2001)

HDAC1^1-3, ttk^rM730 has wing sensillum | ectopic phenotype, enhanceable by Mi-2⁴/Mi-2[+]

(Murawsky et al., 2001)

HDAC1^3-10, ttk^rM730 has wing sensillum | ectopic phenotype, enhanceable by Mi-2⁴/Mi-2[+]

(Murawsky et al., 2001)

HDAC1^5-5, ttk^rM730 has wing sensillum | ectopic phenotype, enhanceable by Mi-2⁴/Mi-2[+]

(Murawsky et al., 2001)

ttk^rM730 has abdominal lateral pentascolopidial chordotonal organ lch5 phenotype, enhanceable by Mi-2⁴

(Murawsky et al., 2001)

Suppressed by

Statement

Reference

ttk^rM730 has eye | somatic clone phenotype, suppressible by lz^mr2

(Siddall et al., 2009)

ttk^rM730 has ommatidium | somatic clone phenotype, suppressible by lz^mr2

(Siddall et al., 2009)

ttk^rM730 has interommatidial bristle | somatic clone phenotype, suppressible by lz^mr2

(Siddall et al., 2009)

hry⁴¹/h[+], ttk^rM730 has wing margin bristle | ectopic phenotype, suppressible by sc[+]/sc¹

(Badenhorst et al., 2002)

hry⁴¹/h[+], ttk^rM730 has wing margin bristle | ectopic phenotype, suppressible by sc^ase-1/sc[+]

(Badenhorst et al., 2002)

Enhancer of

Statement

Reference

ttk[+]/ttk^rM730 is an enhancer of wing margin bristle | ectopic phenotype of ac^Hw-49c

(Badenhorst et al., 2002)

ttk[+]/ttk^rM730 is an enhancer of wing margin bristle | ectopic phenotype of ac^Hw-1

(Badenhorst et al., 2002)

ttk[+]/ttk^rM730 is an enhancer of phenotype of aop^A141

(Lai et al., 1996)

ttk^rM730 is an enhancer of eye phenotype of Ras85D^V12

(Lai et al., 1996)

Other

Statement

Reference

CtBP^87De-10, ttk^rM730 has wing margin bristle | ectopic phenotype

(Badenhorst et al., 2002)

emc^ip15, ttk^rM730 has wing margin bristle | ectopic phenotype

(Badenhorst et al., 2002)

hry⁴¹/h[+], ttk^rM730 has wing margin bristle | ectopic phenotype

(Badenhorst et al., 2002)

emc^E12, ttk^rM730 has wing margin bristle | ectopic phenotype

(Badenhorst et al., 2002)

emc^ip15, ttk[+]/ttk^rM730 has wing margin bristle | ectopic phenotype

(Badenhorst et al., 2002)

hry⁴¹, ttk[+]/ttk^rM730 has wing margin bristle | ectopic phenotype

(Badenhorst et al., 2002)

HDAC1^3-10, ttk^rM730 has wing sensillum | ectopic phenotype

(Murawsky et al., 2001)

HDAC1^5-5, ttk^rM730 has wing sensillum | ectopic phenotype

(Murawsky et al., 2001)

HDAC1^1-3, ttk^rM730 has wing sensillum | ectopic phenotype

(Murawsky et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The eye phenotype observed in ttk^rM730 clones is partially suppressed in a lz^mr2 mutant background. Ommatidial structures and sensory bristles are present, and the eye appears less scarred.

(Siddall et al., 2009)

The addition of ttk^rM730/+ to ac^Hw-49c/+ animals leads to an 1.35 enhancement of the ectopic wing margin bristle phenotype. The addition of ttk^rM730/+ to ac^Hw-1/+ animals leads to an 1.4 enhancement of the ectopic wing margin bristle phenotype. The addition of ttk^rM730/+ to emc^E12/+ or emc^ip15/+ animals leads to a weak ectopic wing margin bristle phenotype. The addition of ttk^rM730/+ to h⁴¹/+ animals leads to a ectopic wing margin bristle phenotype. The addition of sc^ase-1/Y to h⁴¹/+, ttk^rM730/+ animals leads to a complete suppression of the ectopic margin bristle phenotype. The addition ofsc¹/Y to h⁴¹/+, ttk^rM730/+ animals leads to an almost complete suppression of the ectopic margin bristle phenotype. The addition of Df(3R)awd-KRB to h⁴¹/+, ttk^rM730/+ animals leads to a suppression of the ectopic margin bristle phenotype. CtBP^87De-10 combined with ttk^rM730 exhibit an ectopic margin bristle phenotype.

(Badenhorst et al., 2002)

The partial lethality due to run^Scer\UAS.cLa; Scer\GAL4^nos.PG (3% viable) is not suppressed by maternal heterozygosity for ttk^rM730.

(Wheeler et al., 2002)

The number of neurons associated with the pentascolopidial sensory organ in ttk^rM730 mutant embryos is significantly increased if they are also mutant for Mi-2⁴. ttk^rM730 Rpd3^1-3 double mutants have ectopic sensory bristles on the wing veins. ttk^rM730 Rpd3^5-5 double mutants have ectopic sensory bristles on the wing veins. ttk^rM730 Rpd3^3-10 double mutants have ectopic sensory bristles on the wing veins.

(Murawsky et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

ttk¹

(Lai and Li, 1999)

Comments

65% of the ommatidia in ttk¹/ttk^rM730 flies were wild-type, compared to 50-60% in ttk¹ homozygotes. There are 65% of normal ommatidia in ttk¹/ttk^rM730 flies and most mutant ommatidia contain ectopic photoreceptors.

(Lai and Li, 1999)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

42215

w^*; P{neoFRT}82B P{PZ}ttk^rM730/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

E(yan)100D^rM730

(Lai et al., 1996)

ttk^M730

(Giesen et al., 1997)

ttk^RM730

(Wheeler et al., 2002)

ttk^rM730

(Araujo et al., 2007, Vander Zwan et al., 2003)

ttk^rm730

(Siddall et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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