FlyBase Allele Report: Dmel\tkv[Q199D.UAS.Tag:HA]

General Information

Symbol

Dmel\tkv^{Q199D.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0051066

Feature type

allele

Associated gene

Dmel\tkv

Associated Insertion(s)

Carried in Construct

P{UAS-tkv^Q199D}

Also Known As

UAS-TkvA, UAS-tkv^QD, UAS-tkv^Act, UAS-Tkv^Q199D, TKV-A, UAS.CA-Tkv

Key Links

Transgenic product class

missense_variant

(Hoodless et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Hoodless et al., 1996)

Mutagen

in vitro construct

(Haerry et al., 1998, Newfeld et al., 1997, Hoodless et al., 1996)

Progenitor genotype

tkv^Q199D

(Hoodless et al., 1996)

Carried in construct

P{UAS-tkv^Q199D}

Cytology

Description

UASt regulatory sequences drive expression of a constitutively active form of tkv (carries amino acid replacement Q199D) that is tagged with the Tag:HA epitope.

(Hoodless et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

tkv

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

(O'Connor-Giles et al., 2008)

abnormal neuroanatomy | somatic clone | third instar larval stage, with Scer\GAL4^elav.PH

(Haussmann et al., 2008)

abnormal neurophysiology, with Scer\GAL4^ChAT.7.4

(Baines, 2004)

abnormal neurophysiology | larval stage, with Scer\GAL4^BG380

(Sun et al., 2012)

abnormal size | adult stage, with Scer\GAL4^dsx.KI

(Redhai et al., 2016)

abnormal size | adult stage, with Scer\GAL4^esg.PU

(Redhai et al., 2016)

abnormal size | adult stage, with Scer\GAL4^NP0261

(Redhai et al., 2016)

increased cell size, with Scer\GAL4^dsx.KI

(Corrigan et al., 2014)

visible, with Scer\GAL4^A9

(Haerry, 2010, Takaesu et al., 2006, Yu et al., 2000, Haerry et al., 1998)

visible, with Scer\GAL4^unspecified

(Hoodless et al., 1996)

visible | recessive

(Evans et al., 2009)

Phenotype Manifest In

adherens junction & embryonic dorsal trunk, with Scer\GAL4^btl.B123

(Ribeiro et al., 2004)

adherens junction & embryonic dorsal trunk, with Scer\GAL4^btl.PS

(Ribeiro et al., 2004)

dorsal row of triple row of wing sensilla, with Scer\GAL4^A9

(Haerry et al., 1998)

embryonic/larval neuromuscular junction | somatic clone, with Scer\GAL4^elav.PH

(Haussmann et al., 2008)

late endosome | increased number, with Scer\GAL4^NP0261

(Corrigan et al., 2014)

lysosome | adult stage, with Scer\GAL4^dsx.KI

(Redhai et al., 2016)

lysosome | adult stage, with Scer\GAL4^NP0261

(Redhai et al., 2016)

male accessory gland secondary cell, with Scer\GAL4^dsx.KI

(Redhai et al., 2016, Corrigan et al., 2014)

male accessory gland secondary cell, with Scer\GAL4^esg.PU

(Redhai et al., 2016)

male accessory gland secondary cell, with Scer\GAL4^NP0261

(Redhai et al., 2016, Corrigan et al., 2014)

medial row of triple row of wing sensilla, with Scer\GAL4^A9

(Haerry et al., 1998)

multivesicular body, with Scer\GAL4^NP0261

(Corrigan et al., 2014)

multivesicular body | adult stage, with Scer\GAL4^dsx.KI

(Redhai et al., 2016)

multivesicular body | adult stage, with Scer\GAL4^NP0261

(Redhai et al., 2016)

neuromuscular junction, with Scer\GAL4^Mhc.PW

(Dudu et al., 2006)

NMJ bouton | larval stage | increased number, with Scer\GAL4^elav-C155

(O'Connor-Giles et al., 2008)

NMJ bouton | somatic clone, with Scer\GAL4^elav.PH

(Haussmann et al., 2008)

nucleus | adult stage, with Scer\GAL4^dsx.KI

(Redhai et al., 2016)

nucleus | adult stage, with Scer\GAL4^esg.PU

(Redhai et al., 2016)

nucleus | adult stage, with Scer\GAL4^NP0261

(Redhai et al., 2016)

secretory vesicle | adult stage, with Scer\GAL4^dsx.KI

(Redhai et al., 2016)

tracheal dorsal trunk primordium, with Scer\GAL4^btl.PS

(Ribeiro et al., 2004)

ventral row of triple row of wing sensilla, with Scer\GAL4^A9

(Haerry et al., 1998)

wing, with Scer\GAL4^A9

(Haerry, 2010, Takaesu et al., 2006, Yu et al., 2000, Haerry et al., 1998)

wing, with Scer\GAL4^T80

(Takaesu et al., 2012)

wing, with Scer\GAL4^unspecified

(Hoodless et al., 1996)

wing cell

(Evans et al., 2009)

wing cell, with Scer\GAL4^A9

(Haerry et al., 1998)

wing vein

(Evans et al., 2009)

wing vein, with Scer\GAL4^A9

(Haerry, 2010)

wing vein | ectopic, with Scer\GAL4^A9

(Takaesu et al., 2006)

Detailed Description

Statement

Reference

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^esg.PU, Scer\GAL4^dsx.KI or Scer\GAL4^spi-NP0261 (in combination with Gal80[ts] for the temporal regulation of expression) leads to a significant decrease in the relative and absolute size of secondary cell nuclei compared to neighboring main cells and wild-type controls; adulthood-only expression under the combined control of Scer\GAL4^esg.PU and Gal80[ts] does not affect the size of main cell nuclei compared to controls. The adulthood-only expression under the control of Scer\GAL4^dsx.KI (and Gal80[ts]) leads to a significant increase in the number of secretory vesicles, associated with a significant increase in the size of the largest multivesicular body/lysosome, within accessory gland secondary cells compared to controls; there is also a severe decrease in the secretion of (CD63-positive) exosomes into the accessory gland lumen. The adulthood-only expression under the control of Scer\GAL4^spi-NP0261 (and Gal80[ts]) leads to a significant increase in the size of the largest multivesicular body/lysosome within accessory gland secondary cells, as compared to controls.

(Redhai et al., 2016)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^elav.PU does not lead to significant increases in satellite bouton number at the neuromuscular junction of third instar larvae.

(Carrillo et al., 2015)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^dsx.KI results in hypertrophy of the male accessory gland secondary cells. Secondary cells expressing tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^spi-NP0261 contain fewer, but much larger, maturing multivesicular bodies or lysosomes (mMVBLs) compared to controls. The number of immature late endosomes (iLEs) per secondary cell is increased and more of these cells than controls contain multiple secretory vacuoles (SVs) clustered around the enlarged mMVML.

(Corrigan et al., 2014)

The amplitude of miniature excitatory junctional currents (mEJCs) is normal at the neuromuscular junction (NMJ) in larvae expressing tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^BG380, but the evoked EJC shows an increase in amplitude compared to wild type, resulting in an increase in quantal content at the mutant NMJ.

(Sun et al., 2012)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} in the wing discs under the control of Scer\GAL4^T80 leads to ectopic wing vein formation.

(Takaesu et al., 2012)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 results in small wings that are highly pigmented with excess vein tissue.

(Haerry, 2010)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} in the wing with Scer\GAL4^ptc-559.1 induces patterning abnormalities including extensive ectopic vein, fusion of longitudinal veins, and loss of intervein regions.

(Evans et al., 2009)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^BG380 does not significantly alter the number of boutons per muscle surface area at the neuromuscular junction of third instar larvae.

(Merino et al., 2009)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PH in clones induced by FLP/FRT mediated recombination (to activate the Scer\GAL4^elav.PH driver) results in an increase in the number of type 1b boutons at muscle 13 compared to wild type.

(Haussmann et al., 2008)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PU does not affect synaptic growth at the larval neuromuscular junction (the average number of satellite boutons is not significantly different from wild type).

Expression of two copies of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 results in an increase in both the total number of boutons and the number of satellite boutons at the larval neuromuscular junction.

(O'Connor-Giles et al., 2008)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Mhc.PW results in the ratio of import/export rates being higher than in control muscles (7.4 compared to 3.6). This difference in the import/export ratio explains the net accumulation of nuclear Mad in tkv^{Q199D.Scer\UAS.T:Ivir\HA1} muscles above the levels of control in steady state. The effective import rate does not increase, but decreases slightly upon constitutive signalling (1.6 times smaller in tkv^{Q199D.Scer\UAS.T:Ivir\HA1} than in controls), while it is the effective export rate that significantly decreases (3.4 times smaller in

tkv^{Q199D.Scer\UAS.T:Ivir\HA1}).

Constitutive signalling caused by expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Mhc.PW leads to an increase by an order of magnitude (67%) of the immobile pool of Mad in the nucleus.

(Dudu et al., 2006)

100% of wings are overgrown and have numerous ectopic veins in flies expressing tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9.

(Takaesu et al., 2006)

Expression of tkv^{Q199D.UAS.Tag:HA} in presynaptic cholinergic neurons, driven by Scer\GAL4^ChAT.7.4, is sufficient to produce a large and significant increase in synaptic current amplitude in aCC/RP2 neurons compared to controls. tkv^{Q199D.UAS.Tag:HA} expression in postsynaptic neurons, driven by Scer\GAL4^eve.RN2, has no effect on synaptic current amplitude.

(Baines, 2004)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PLu or Scer\GAL4^OK6 in a wild-type background does not result in a significant increase in the number of neuromuscular junction synaptic boutons.

(McCabe et al., 2004)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^btl.PS in the tracheal system results in the formation of fine dorsal trunks containing stretches of autocellular adherens junctions. Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^btl.B123 in single cells or groups of cells in the dorsal trunk results in the formation of ectopic autocellular adherens junctions, although at a low frequency.

(Ribeiro et al., 2004)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 causes a wing phenotype.

(Yu et al., 2000)

Flies expressing tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 have small wings which are blistered due to lack of adhesion of the dorsal and ventral compartments. Intervein cells express vein-specific hairs and the marginal triple row bristles are transformed into the oversized double row bristles normally seen at more distal positions along the wing margin. The wings are darkly pigmented.

(Haerry et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^BG380, sax^A.UAS.cBa, tkv^{Q199D.UAS.Tag:HA} has abnormal neurophysiology | third instar larval stage phenotype, suppressible by trio^6A/trio^S137203

(Ball et al., 2010)

Scer\GAL4^elav-C155, tkv^{Q199D.UAS.Tag:HA} has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^elav-C155/nwk^UAS.cCa

(O'Connor-Giles et al., 2008)

Scer\GAL4^elav-C155, tkv^{Q199D.UAS.Tag:HA} has abnormal neuroanatomy | larval stage phenotype, suppressible by Dap160^UAS.cKa, Scer\GAL4^elav-C155

(O'Connor-Giles et al., 2008)

Scer\GAL4^A9, tkv^{Q199D.UAS.Tag:HA} has visible phenotype, suppressible by Snoo^UAS.cTa/Scer\GAL4^A9

(Takaesu et al., 2006)

Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/tkv^{Q199D.UAS.Tag:HA} is an enhancer of abnormal neuroanatomy | larval stage phenotype of Scer\GAL4^elav-C155, Snx16^3A.UAS.GFP

(Rodal et al., 2011)

Scer\GAL4^elav.PU/tkv^{Q199D.UAS.Tag:HA} is an enhancer of abnormal neuroanatomy | larval stage phenotype of nwk²

(O'Connor-Giles et al., 2008)

tkv^{Q199D.UAS.Tag:HA}/Scer\GAL4^elav.PLu is an enhancer of abnormal neuroanatomy phenotype of hiw^EMS

(McCabe et al., 2004)

Suppressor of

Statement

Reference

Scer\GAL4^BG380/tkv^{Q199D.UAS.Tag:HA} is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of nmo^unspecified

(Merino et al., 2009)

NOT Suppressor of

Statement

Reference

Snx16^3A.UAS.GFP/tkv^{Q199D.UAS.Tag:HA}, Scer\GAL4^VGlut1.PD is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Snx16^3A.UAS.GFP/tkv^{Q199D.UAS.Tag:HA}, Scer\GAL4^VGlut1.PD is a non-suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Other

Statement

Reference

Scer\GAL4^VGlut1.PD, Snx16^3A.UAS.GFP, tkv^{Q199D.UAS.Tag:HA} has abnormal neuroanatomy | third instar larval stage phenotype

(Deshpande et al., 2016)

Scer\GAL4^elav.PU, dpr11^MI02231, tkv^{Q199D.UAS.Tag:HA} has abnormal neuroanatomy | third instar larval stage phenotype

(Carrillo et al., 2015)

DIP-γ^MI03222, Scer\GAL4^elav.PU, tkv^{Q199D.UAS.Tag:HA} has abnormal neuroanatomy | third instar larval stage phenotype

(Carrillo et al., 2015)

Scer\GAL4^{VP16.nanos.UTR}, sax^{Q263D.UAS.Tag:HA}, tkv^{Q199D.UAS.Tag:HA} has increased cell number | adult stage phenotype

(Smendziuk et al., 2015)

Scer\GAL4^BG380, sax^A.UAS.cBa, tkv^{Q199D.UAS.Tag:HA} has abnormal neurophysiology | third instar larval stage phenotype

(Ball et al., 2010)

kek5^UAS.GFP, tkv^{Q199D.UAS.Tag:HA} has visible | recessive phenotype

(Evans et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

tkv^{Q199D.UAS.Tag:HA} has wing cell phenotype, enhanceable by kek5^UAS.GFP

(Evans et al., 2009)

tkv^{Q199D.UAS.Tag:HA} has wing vein phenotype, enhanceable by kek5^UAS.GFP

(Evans et al., 2009)

NOT Enhanced by

Statement

Reference

Scer\GAL4^A9, tkv^{Q199D.UAS.Tag:HA} has wing phenotype, non-enhanceable by Scer\GAL4^A9/sog^{supersog1.UAS}

(Yu et al., 2000)

Suppressed by

Statement

Reference

Scer\GAL4^T80, tkv^{Q199D.UAS.Tag:HA} has wing phenotype, suppressible by Scer\GAL4^T80, tkv^{Q199D.UAS.Tag:HA}

(Takaesu et al., 2012)

Scer\GAL4^elav-C155, tkv^{Q199D.UAS.Tag:HA} has NMJ bouton | increased number | larval stage phenotype, suppressible by Scer\GAL4^elav-C155/nwk^UAS.cCa

(O'Connor-Giles et al., 2008)

Scer\GAL4^elav-C155, tkv^{Q199D.UAS.Tag:HA} has NMJ bouton | increased number | larval stage phenotype, suppressible by Dap160^UAS.cKa, Scer\GAL4^elav-C155

(O'Connor-Giles et al., 2008)

Scer\GAL4^A9, tkv^{Q199D.UAS.Tag:HA} has wing phenotype, suppressible by Snoo^UAS.cTa/Scer\GAL4^A9

(Takaesu et al., 2006)

Scer\GAL4^A9, tkv^{Q199D.UAS.Tag:HA} has wing vein | ectopic phenotype, suppressible by Snoo^UAS.cTa/Scer\GAL4^A9

(Takaesu et al., 2006)

NOT suppressed by

Statement

Reference

Scer\GAL4^A9, tkv^{Q199D.UAS.Tag:HA} has wing phenotype, non-suppressible by Scer\GAL4^A9/sog^{supersog1.UAS}

(Yu et al., 2000)

Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/tkv^{Q199D.UAS.Tag:HA} is an enhancer of NMJ bouton | larval stage | increased number phenotype of Scer\GAL4^elav-C155, Snx16^3A.UAS.GFP

(Rodal et al., 2011)

Scer\GAL4^elav.PU/tkv^{Q199D.UAS.Tag:HA} is an enhancer of NMJ bouton | larval stage | increased number phenotype of nwk²

(O'Connor-Giles et al., 2008)

tkv^{Q199D.UAS.Tag:HA}/Scer\GAL4^elav.PLu is an enhancer of bouton | increased number phenotype of hiw^EMS

(McCabe et al., 2004)

tkv^{Q199D.UAS.Tag:HA}/Scer\GAL4^elav.PLu is an enhancer of neuromuscular junction phenotype of hiw^EMS

(McCabe et al., 2004)

Suppressor of

Statement

Reference

Scer\GAL4^T80, tkv^{Q199D.UAS.Tag:HA} is a suppressor of wing phenotype of Scer\GAL4^T80, tkv^{Q199D.UAS.Tag:HA}

(Takaesu et al., 2012)

Scer\GAL4^{VP16.nanos.UTR}/tkv^{Q199D.UAS.Tag:HA} is a suppressor of wing vein phenotype of magu^e00439/magu^f02256

(Zheng et al., 2011)

Scer\GAL4^{VP16.nanos.UTR}/tkv^{Q199D.UAS.Tag:HA} is a suppressor of testis phenotype of magu^e00439/magu^f02256

(Zheng et al., 2011)

Scer\GAL4^{VP16.nanos.UTR}/tkv^{Q199D.UAS.Tag:HA} is a suppressor of male germline stem cell | third instar larval stage phenotype of magu^e00439/magu^f02256

(Zheng et al., 2011)

Scer\GAL4^BG380/tkv^{Q199D.UAS.Tag:HA} is a suppressor of NMJ bouton | third instar larval stage phenotype of nmo^unspecified

(Merino et al., 2009)

tkv^{Q199D.UAS.Tag:HA}/Scer\GAL4^hs.PB is a suppressor of embryonic dorsal epidermis | germline clone | rescuable maternal effect phenotype of Cka⁰⁵⁸³⁶

(Chen et al., 2002)

Scer\GAL4^LE/tkv^{Q199D.UAS.Tag:HA} is a suppressor of embryonic epidermis | dorsal phenotype of arm³

(McEwen et al., 2000)

Scer\GAL4^pnr-MD237/tkv^{Q199D.UAS.Tag:HA} is a suppressor of embryonic dorsal epidermis phenotype of Jra^76-19

(Hou et al., 1997)

NOT Suppressor of

Statement

Reference

Snx16^3A.UAS.GFP/tkv^{Q199D.UAS.Tag:HA}, Scer\GAL4^VGlut1.PD is a non-suppressor of NMJ bouton | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

tkv^{Q199D.UAS.Tag:HA}/Scer\GAL4^T80 is a non-suppressor of wing phenotype of Scer\GAL4^T80, fuss^UAS.cTa

(Takaesu et al., 2012)

Other

Statement

Reference

Scer\GAL4^VGlut1.PD, Snx16^3A.UAS.GFP, tkv^{Q199D.UAS.Tag:HA} has NMJ bouton | third instar larval stage phenotype

(Deshpande et al., 2016)

DIP-γ^MI03222, Scer\GAL4^elav.PU, tkv^{Q199D.UAS.Tag:HA} has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Carrillo et al., 2015)

DIP-γ^MI03222, Scer\GAL4^elav.PU, tkv^{Q199D.UAS.Tag:HA} has NMJ bouton | increased number | third instar larval stage phenotype

(Carrillo et al., 2015)

Scer\GAL4^elav.PU, dpr11^MI02231, tkv^{Q199D.UAS.Tag:HA} has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Carrillo et al., 2015)

Scer\GAL4^elav.PU, dpr11^MI02231, tkv^{Q199D.UAS.Tag:HA} has NMJ bouton | increased number | third instar larval stage phenotype

(Carrillo et al., 2015)

Scer\GAL4^{VP16.nanos.UTR}, sax^{Q263D.UAS.Tag:HA}, tkv^{Q199D.UAS.Tag:HA} has somatic cell of testis phenotype

(Smendziuk et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Third instar larvae expressing tkv^{Q199D.Scer\UAS.T:Ivir\HA1} and Snx16^{3A.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^VGlut.PD display increased number of boutons on neuromuscular junctions.

(Deshpande et al., 2016)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^elav.PU in a dpr11^MI02231/+ or DIP-γ^MI03222/+ background leads to extra satellite boutons at the neuromuscular junction (NMJ) of third instar larvae.

(Carrillo et al., 2015)

Over-activation of the BMP pathway, by co-expressing tkv^{Q199D.Scer\UAS.T:Ivir\HA1} and sax^{Q263D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, leads to an increase of early somatic cells in the male gonad, but no increase in somatic cell proliferation.

(Smendziuk et al., 2015)

Co-expression of CORL^Scer\UAS.cTa in the wing disc under the control of Scer\GAL4^T80 suppresses ectopic wing vein formation from CORL^Scer\UAS.cTa expression. These wings exhibit a few ectopic veins and truncations of L2, L4 and L5 wing veins.

(Takaesu et al., 2012)

Co-expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} dramatically enhances the increase in satellite bouton number at the neuromuscular junction which is seen in larvae expressing Snx16^{3A.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav-C155.

(Rodal et al., 2011)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} using the germ cell driver Scer\GAL4^{nos.UTR.T:Hsim\VP16} raises the fraction of testes with germline stem cells from 63% to 100% in a magu^e00439/magu^f02256 background. The median germline stem cell number also doubles compared to that observed in mutants.

(Zheng et al., 2011)

Co-expression of sax^{A.Scer\UAS.cBa} and tkv^{Q199D.Scer\UAS.T:Ivir\HA1} in motor neurons under the control of Scer\GAL4^BG380 produces increased mean evoked excitatory junctional potentials (EJPs) and quantal content compared to controls. The size of miniature excitatory junctional potentials (mEJPs) does not differ from controls.

trio^S137203/trio^6A suppresses the increase in evoked excitatory junctional potential (EJPs) and quantal content seen when sax^{A.Scer\UAS.cBa} and tkv^{Q199D.Scer\UAS.T:Ivir\HA1} are expressed in motor neurons under the control of Scer\GAL4^BG380.

(Ball et al., 2010)

The wing patterning phenotypes resulting from the expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} with Scer\GAL4^ptc-559.1 are enhanced by the co-expression of kek5^{Scer\UAS.T:Avic\GFP}, and there is an additional decrease in wing size.

(Evans et al., 2009)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^BG380 significantly suppresses the reduction in the number of boutons per muscle surface area which is seen at the neuromuscular junction in nmo^unspecified third instar larvae.

(Merino et al., 2009)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PH in ewg² motorneurons (these motorneurons have been obtained by using FLP/FRT mediated recombination to inactivate the ewg^elav.PH rescuing transgene and simultaneously activate the Scer\GAL4^elav.PH driver in a ewg² background) results in intermediate numbers of boutons at the larval neuromuscular junction compared to wild-type and ewg² motorneurons.

(Haussmann et al., 2008)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PU enhances the increase in the average number of satellite boutons at the neuromuscular junction that is seen in nwk² larvae.

Co-expression of nwk^Scer\UAS.cCa suppresses the increase in the total number of boutons and in the number of satellite boutons at the neuromuscular junction that is seen in larvae expressing two copies of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155.

Expression of one copy of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 in a Dap160^Δ1/+ background results in an increase in both the total number of boutons and the number of satellite boutons at the larval neuromuscular junction compared to wild type.

Co-expression of Dap160^Scer\UAS.cKa suppresses the increase in the total number of boutons and in the number of satellite boutons at the neuromuscular junction that is seen in larvae expressing two copies of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155.

(O'Connor-Giles et al., 2008)

The wing phenotypes caused by expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 are suppressed by co-expression of Snoo^Scer\UAS.cTa; the wing in the co-expressing flies appears similar to the phenotype seen in flies expressing Snoo^Scer\UAS.cTa alone under the control of Scer\GAL4^A9.

(Takaesu et al., 2006)

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PLu in a hiw^EMS background results in a 30% increase in the number of boutons per muscle surface area at the neuromuscular junction compared to hiw^EMS single mutants.

(McCabe et al., 2004)

The dorsal open phenotype seen in homozygous Cka⁰⁵⁸³⁶ embryos derived from females carrying homozygous Cka⁰⁵⁸³⁶ germ-line clones is substantially rescued by expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.PB using heat shock.

(Chen et al., 2002)

Scer\GAL4^pnr-MD237-mediated expression in Jra^76-19 embryos rescues the dorsal open phenotype.

(Hou et al., 1997)

Similar dominant wing phenotypes are observed in flies expressing dpp^Scer\UAS.cHa and tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^unspecified that are not observed in flies expressing tkv^Scer\UAS.cHa under the control of Scer\GAL4^unspecified.

(Hoodless et al., 1996)

Xenogenetic Interactions

Statement

Reference

The reduced number of boutons on neuromuscular junctions as well as crawling defects observed in third instar larvae expressing Hsap\TARDBP^Scer\UAS.cHa under the Scer\GAL4^VGlut.PD driver cannot be restored by either by simultaneous co-expression of Snx16^{3A.Scer\UAS.T:Avic\GFP} and tkv^{Q199D.Scer\UAS.T:Ivir\HA1}. Co-expressing Rab5^{S43N.Scer\UAS} also fails to restore the neuromuscular junction growth defects.

(Deshpande et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

604925

w^*; P{UAS-tkv^Q199D}B

604924

y¹ w^*; P{UAS-tkv^Q199D}A2

Notes on Origin

Discoverer

Comments

Constitutively active.

(Hoodless et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

TKV-A

(Dobens et al., 2000)

TKVA

(Haerry et al., 1998)

tkv^{Q199D.Scer\UAS.T:Ivir\HA1}

tkv^{Q199D.UAS.T:HA1}

tkv^{Q199D.UAS.Tag:HA}

tkv^Q199D

(Rodal et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (43)

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