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General Information
Symbol
Dmel\smoD16
Species
D. melanogaster
Name
FlyBase ID
FBal0051131
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
insertion site
Comment:

Insertion of 38 base pairs after codon 124 that introduces two stop codons, one within and the other just downstream of the insertion.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion of 38 base pairs after codon 124 that introduces two stop codons, one within and the other just downstream of the insertion. Codon 124 falls well before the first transmembrane domain.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The size of smoD16 homozygous follicle cell clones in the germarium is significantly smaller than control clones.

Follicle stem cell clones homozygous for smoD16 are present at a much lower frequency than control clones at 7 days (25% vs 73%) and 14 days (6% vs 57%) after clone induction.

Homozygous cells in the morphogenetic furrow (in clones that encompass the morphogenetic furrow) show a significant impairment in apical constriction.

Unlike neutral somatic clones, smoD16 homozygous somatic clones are rapidly lost from the somatic stem cell population of the germarium so that, by 3 weeks after clone induction, none remain.

When smoD16 clones are made in the posterior margin of the eye disc, clones lack photoreceptor differentiation.

Somatic clones of smoD16 in the ocellar triangle (ocellar cuticle) lead to reduced or absent ocelli. The medial ocellus is occasionally split in two.

Cells in clones in the eye disc fail to enter S phase in the second mitotic wave.

Embryos derived from germline clone females exhibit loss of naked cuticle and polarity of the embryo is lost.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
NOT Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference

smoD16 is an enhancer of eye phenotype of hidGMR.PG/WGMR.PG

Suppressor of
Statement
Reference

smoD16 is a suppressor of phenotype of slmbe4-1

smoD16 is a suppressor of phenotype of slmb05415

Additional Comments
Genetic Interactions
Statement
Reference

Eyes mosaic for smoD16 (generated using the ey-FLP/FRT system) enhance the WGMR.PG eye phenotype.

smoD16/+ restores an almost wild-type wing phenotype in animals expressing fuScer\UAS.T:Hsap\Myr2,T:Avic\GFP-CFP under the control of Scer\GAL4Bx-MS1096.

The rapid loss of smoD16 homozygous somatic clone cells from the somatic stem cell population of the germarium is not significantly suppressed if the clone cells are also tkvQ199D.Scer\UAS; Scer\GAL4Act5C.PI (Scer\GAL80 method).

The addition of dppScer\UAS.cSa or eyaScer\UAS.cPa (driven by Scer\GAL4ey.PB) to animals with smoD16 clones in the eye disc has no effect on the photoreceptor differentiation phenotype seen in eye discs. The delayed progression of the morphogenetic furrow is also not affected. The addition of soScer\UAS.cPa and eyaScer\UAS.cPa (driven by Scer\GAL4ey.PB) to animals with smoD16 clones in the eye disc has no effect on the photoreceptor differentiation phenotype seen in eye discs. The delayed progression of the morphogenetic furrow is also not affected. The addition of dppScer\UAS.cSa and eyaScer\UAS.cPa (driven by Scer\GAL4ey.PB) to animals with smoD16 clones in the eye disc, fully suppresses photoreceptor differentiation phenotype seen in eye discs. The delayed progression of the morphogenetic furrow is also rescued. If soScer\UAS.cPa is also added, posterior margin clones are still rescued, although ectopic anterior furrows are induced with high frequency. The addition of soScer\UAS.cPa and dppScer\UAS.cSa (driven by Scer\GAL4ey.PB) to animals with smoD16 clones in the eye disc has no effect on the photoreceptor differentiation phenotype seen in eye discs.

Suppresses slmb induced outgrowths in mutant clones.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Not rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Likely to be a null allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (27)