Allele Dmel\salm^Scer\UAS.cKa

General Information
Symbol	Dmel\salm^Scer\UAS.cKa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Kuhnlein	FlyBase ID	FBal0051183
Feature type	allele	Associated gene	Dmel\salm

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Kuhnlein and Schuh, 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of salm fragment from nucleotide 370 to nucleotide 5555 of the composite cDNA sequence (FBrf0066960) is governed by Scer\UAS regulatory sequences. (Kuhnlein and Schuh, 1996)
Carried in construct	P{UAS-salm.K} (Bradley and Andrew, 2001, Franch-Marro and Casanova, 2002, Elstob et al., 2001, Domingos et al., 2004, Ribeiro et al., 2004, Chen et al., 1998, Kuhnlein and Schuh, 1996, Sprecher et al., 2007, Caussinus et al., 2008, Schönbauer et al., 2011, Grieder et al., 2009, Bryantsev et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^bi-omb-Gal4 (Grieder et al., 2009) lethal \| pharate adult stage, with Scer\GAL4¹¹⁵¹, Scer\GAL80^ts.αTub84B (Schönbauer et al., 2011) lethal \| pharate adult stage, with Scer\GAL4^Mef2.PR, Scer\GAL80^ts.αTub84B (Schönbauer et al., 2011) lethal \| pupal stage, with Scer\GAL4^dpp.blk1 (Grieder et al., 2009)
Phenotype Manifest In
	abdominal lateral pentascolopidial chordotonal organ lch5, with Scer\GAL4^en-e16E (Elstob et al., 2001) adherens junction & embryonic dorsal branch, with Scer\GAL4^btl.PS (Ribeiro et al., 2004) adherens junction & embryonic ganglionic branch, with Scer\GAL4^btl.PS (Ribeiro et al., 2004) adherens junction & embryonic tracheal system, with Scer\GAL4^btl.PS (Ribeiro et al., 2004) adult somatic muscle, with Scer\GAL4^Mef2.PR, Scer\GAL80^ts.αTub84B (Schönbauer et al., 2011) embryonic/larval dorsal anastomosis, with Scer\GAL4^btl.PS (Chen et al., 1998) embryonic/larval dorsal branch, with Scer\GAL4^btl.PS (Chen et al., 1998) embryonic/larval dorsal trunk, with Scer\GAL4^salm.TSE (Kuhnlein and Schuh, 1996) embryonic/larval lateral trunk, with Scer\GAL4^btl.PS (Franch-Marro and Casanova, 2002) embryonic/larval oenocyte, with Scer\GAL4^en-e16E (Elstob et al., 2001) head, with Scer\GAL4^dpp.blk1 (Grieder et al., 2009) leg, with Scer\GAL4¹¹⁵¹, Scer\GAL80^ts.αTub84B (Schönbauer et al., 2011) leg, with Scer\GAL4^dpp.blk1 (Grieder et al., 2009) leg, with Scer\GAL4^Mef2.PR, Scer\GAL80^ts.αTub84B (Schönbauer et al., 2011) mesothoracic leg disc, with Scer\GAL4^dpp.blk1 (Grieder et al., 2009) mesothoracic tergum, with Scer\GAL4^dpp.blk1 (Grieder et al., 2009) mesothoracic tergum \| ectopic, with Scer\GAL4^bi-omb-Gal4 (Grieder et al., 2009) mesothoracic tergum \| ectopic, with Scer\GAL4^dpp.blk1 (Grieder et al., 2009) third segment of antenna \| ectopic, with Scer\GAL4^dpp.blk1 (Grieder et al., 2009) tracheal primordium, with Scer\GAL4^Kr.PM (Kuhnlein and Schuh, 1996) tracheal system, with Scer\GAL4^Kr.PM (Kuhnlein and Schuh, 1996) unguis, with Scer\GAL4^dpp.blk1 (Grieder et al., 2009) wing, with Scer\GAL4^bi-omb-Gal4 (Grieder et al., 2009) wing, with Scer\GAL4^dpp.blk1 (Grieder et al., 2009) wing \| ectopic, with Scer\GAL4^dpp.blk1 (Grieder et al., 2009) wing disc, with Scer\GAL4^dpp.blk1 (Grieder et al., 2009) wing hinge \| ectopic, with Scer\GAL4^dpp.blk1 (Grieder et al., 2009)
Detailed Description
	Statement Reference Ectopic expression of salm[Scer\UAS.cKa] under the control of Scer\GAL4[Mef2.PR] and Scer\GAL80 induces the transformation of the tubular fibre-type leg muscles into fibrillar indirect flight muscle-like muscles. As a consequence, these transformed leg muscles do not function properly and flies die as pharate adults. Ectopic expression of salm[Scer\UAS.cKa] under the control of Scer\GAL4[1151] and Scer\GAL80 induces the transformation of the tubular fibre-type leg muscles into fibrillar indirect flight muscle-like muscles. As a consequence, these transformed leg muscles do not function properly and flies die as pharate adults. Ectopic expression of salm[Scer\UAS.cKa] under the control of Scer\GAL4[Mef2.PR] and Scer\GAL80 induces the transformation of the tubular fibre-type abdominal muscles into fibrillar indirect flight muscle-like muscles. (Schönbauer et al., 2011) Expression of salm[Scer\UAS.cKa] under the control of Scer\GAL4[dpp.blk1] generally results in early pupal lethality. If a weak driver insertion or lower temperature is used, more pharate adults can be recovered. These pharate adults have a large ectopic notum, which often contain macrochaetae. Severe defects in the legs and wings are seen, as well as head and notum defects. Third instar leg discs expressing salm[Scer\UAS.cKa] under the control of Scer\GAL4[dpp.blk1] and then transplanted into wild-type host larvae of approximately the same age show transformations. 15/16 transplanted second leg discs give rise to mesothoracic notum structures. The legs are strongly truncated (distal structures like claws are completely missing and other leg segments are crippled and difficult to recognise). The sternopleura can be identified in several cases, and occasionally some wing hinge tissue can be seen. 5/16 transplanted second leg discs show transdetermination to proximal wing structures and 4/16 show transdetermination to structures of the third antennal segment. Only 2/14 transplanted first leg discs form ectopic notum structures. The legs are crippled and truncated. No differentiated claws are found and only a few occasional sex comb teeth can be seen. 3/3 transplanted third leg discs result in truncated and malformed leg structures (no notum tissue is seen). Mesothoracic leg discs expressing salm[Scer\UAS.cKa] under the control of Scer\GAL4[dpp.blk1] show extra growth in dorsal cells. Wing discs expressing salm[Scer\UAS.cKa] under the control of Scer\GAL4[dpp.blk1] show outgrowths within the central domain (the region that would normally develop into the wing blade). Only a few larvae expressing salm[Scer\UAS.cKa] under the control of Scer\GAL4[bi-omb-Gal4] develop into pharate adults. These animals either have strongly reduced wings or no wings, and instead a supernumerary notum has formed. (Grieder et al., 2009) Expression of salm^Scer\UAS.cKa under the control of Scer\GAL4^btl.PS in the tracheal system results in autocellular adherens junction (AJ) formation being completely blocked, and all branches are transformed into tubes with cells organised in a paired arrangement and connected via intercellular AJs. Either the cells are not capable of reaching around the lumen or the initial autocellular AJ contacts are not stabilised and zipped up. Single cells expressing salm^Scer\UAS.cKa under the control of Scer\GAL4^btl.B123 in either the dorsal or ventral branches of the tracheal system still form extended autocellular adherens junctions (AJs) (as occurs in wild type). Groups of cells in the dorsal or ganglionic branch expressing salm^Scer\UAS.cKa under the control of Scer\GAL4^btl.B123 are generally inhibited in their capacity to form autocellular AJs and form cell aggregates with intercellular AJs. However, single cells at the periphery of such groups often form autocellular AJs. (Ribeiro et al., 2004) When salm^Scer\UAS.cKa is driven by Scer\GAL4^btl.PS the cells of the lateral trunk of the tracheal system adopt a more dorsal identity. (Franch-Marro and Casanova, 2002) When salm^Scer\UAS.cKa is driven by Scer\GAL4^btl.PS no increase in dorsal trunk cell migration is seen. (Bradley and Andrew, 2001) Expression of salm^Scer\UAS.cKa under the control of Scer\GAL4^en-e16E results in a high frequency of lateral chordotonal organ arrays containing 3-4 chordotonal organs at 25^oC, while at 29^oC 3 chordotonal organs per array are mostly seen. A moderate reduction in oenocyte number is also seen. (Elstob et al., 2001) When expression is driven by Scer\GAL4^btl.PS there is no effect on the primary outgrowth of tracheal dorsal branch progenitor cells. Subsequently these cells fail to grow out to the dorsal midline and lack the typical U-turn structure of wild-type dorsal branches. Dorsal anastomosis formation is blocked. (Chen et al., 1998) When expression is driven by Scer\GAL4^Kr.PM, the number of tracheal primordial cells and the corresponding differentiated tracheal system is reduced. There are no associated defects in the epidermis or peripheral nervous system. When expression is driven by Scer\GAL4^salm.TSE, in an otherwise salm mutant background, the dorsal truck branches fuse at high frequency, leading to dorsal trunk fragments which span several tracheal metameric units, constituting a partial rescue of the salm mutant phenotype. (Kuhnlein and Schuh, 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement Reference salm^Scer\UAS.cKa/Scer\GAL4¹¹⁵¹ is a suppressor of dorsal medial muscle phenotype of vg^null (Schönbauer et al., 2011) Scer\GAL4^en-e16E/salm^Scer\UAS.cKa is a suppressor of abdominal lateral pentascolopidial chordotonal organ lch5 \| supernumerary phenotype of Scer\GAL4^en-e16E, spi^s.Scer\UAS (Elstob et al., 2001)
NOT Suppressor of
Statement Reference Scer\GAL4^en-e16E/salm^Scer\UAS.cKa is a non-suppressor of embryonic/larval oenocyte \| supernumerary phenotype of Scer\GAL4^en-e16E, spi^s.Scer\UAS (Elstob et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference Expression of salm[Scer\UAS.cKa] under the control of Scer\GAL4[1151] restores the normal muscle fibre structure in dorsal longitudinal indirect flight muscles in vg[null] mutants. Fibrillar transformation of leg muscles is not observed as a result of salm[Scer\UAS.cKa] expression. (Schönbauer et al., 2011) The addition of salm^Scer\UAS.cKa and Scer\GAL4^btl.PS to rib¹ embryos does not rescue the dorsal trunk migration phenotype. (Bradley and Andrew, 2001) Coexpression of spi^s.Scer\UAS and salm^Scer\UAS.cKa under the control of Scer\GAL4^en-e16E results in a chordotonal phenotype identical to that seen when salm^Scer\UAS.cKa alone is expressed under the control of Scer\GAL4^en-e16E. Giant clusters of oenocyte cells are induced dorsally, as is seen when spi^s.Scer\UAS alone is expressed under the control of Scer\GAL4^en-e16E. (Elstob et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to rescue	Scer\GAL4^btl.PS/salm^Scer\UAS.cKa fails to rescue salm¹ (Bradley and Andrew, 2001)
Comments
Stocks ( 2 )
Bloomington	29715 w^; P{UAS-salm.K}X; P{UAS-mCD8::GFP.L}LL5 29716 w^; P{UAS-salm.K}B3/CyO, P{en1}wg^en11; P{UAS-GFP.GB}3/TM6B, Tb¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	salm^Scer\UAS.cKa salm^UAS.cKa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Kuhnlein (Kuhnlein and Schuh, 1996)
Secondary FlyBase IDs

References ( 12 )
Research paper	Bryantsev et al., 2012, Dev. Cell 23(3): 664--673 Extradenticle and homothorax control adult muscle fiber identity in Drosophila. [FBrf0219427] Schönbauer et al., 2011, Nature 479(7373): 406--409 Spalt mediates an evolutionarily conserved switch to fibrillar muscle fate in insects. [FBrf0216735] Grieder et al., 2009, Dev. Biol. 329(2): 315--326 Spalt major controls the development of the notum and of wing hinge primordia of the Drosophila melanogaster wing imaginal disc. [FBrf0210004] Caussinus et al., 2008, Curr. Biol. 18(22): 1727--1734 Tip-Cell Migration Controls Stalk-Cell Intercalation during Drosophila Tracheal Tube Elongation. [FBrf0206360] Sprecher et al., 2007, Genes Dev. 21(17): 2182--2195 Adult and larval photoreceptors use different mechanisms to specify the same Rhodopsin fates. [FBrf0205916] Domingos et al., 2004, Dev. Biol. 273(1): 121--133 Regulation of R7 and R8 differentiation by the spalt genes. [FBrf0179729] Ribeiro et al., 2004, Curr. Biol. 14(24): 2197--2207 Genetic control of cell intercalation during tracheal morphogenesis in Drosophila. [FBrf0183816] Franch-Marro and Casanova, 2002, Dev. Biol. 250(2): 374--382 spalt-induced specification of distinct dorsal and ventral domains is required for Drosophila tracheal patterning. [FBrf0151884] Bradley and Andrew, 2001, Development 128(15): 3001--3015 ribbon encodes a novel BTB/POZ protein required for directed cell migration in Drosophila melanogaster. [FBrf0138367] Elstob et al., 2001, Development 128(5): 723--732 spalt-dependent switching between two cell fates that are induced by the Drosophila EGF receptor. [FBrf0134578] Chen et al., 1998, Development 125(24): 4959--4968 The transcription factors KNIRPS and KNIRPS RELATED control cell migration and branch morphogenesis during Drosophila tracheal development. [FBrf0105788] Kuhnlein and Schuh, 1996, Development 122(7): 2215--2223 Dual function of the region-specific homeotic gene spalt during Drosophila tracheal system development. [FBrf0088351]

Allele Dmel\salmScer\UAS.cKa

Allele Dmel\salm^Scer\UAS.cKa