|Name||Saccharomyces cerevisiae UAS construct a of Kuhnlein||FlyBase ID||FBal0051183|
|Feature type||allele||Associated gene||Dmel\salm|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
Ectopic expression of salm[Scer\UAS.cKa] under the control of Scer\GAL4[Mef2.PR] and Scer\GAL80 induces the transformation of the tubular fibre-type leg muscles into fibrillar indirect flight muscle-like muscles. As a consequence, these transformed leg muscles do not function properly and flies die as pharate adults. Ectopic expression of salm[Scer\UAS.cKa] under the control of Scer\GAL4 and Scer\GAL80 induces the transformation of the tubular fibre-type leg muscles into fibrillar indirect flight muscle-like muscles. As a consequence, these transformed leg muscles do not function properly and flies die as pharate adults. Ectopic expression of salm[Scer\UAS.cKa] under the control of Scer\GAL4[Mef2.PR] and Scer\GAL80 induces the transformation of the tubular fibre-type abdominal muscles into fibrillar indirect flight muscle-like muscles.
Expression of salm[Scer\UAS.cKa] under the control of Scer\GAL4[dpp.blk1] generally results in early pupal lethality. If a weak driver insertion or lower temperature is used, more pharate adults can be recovered. These pharate adults have a large ectopic notum, which often contain macrochaetae. Severe defects in the legs and wings are seen, as well as head and notum defects. Third instar leg discs expressing salm[Scer\UAS.cKa] under the control of Scer\GAL4[dpp.blk1] and then transplanted into wild-type host larvae of approximately the same age show transformations. 15/16 transplanted second leg discs give rise to mesothoracic notum structures. The legs are strongly truncated (distal structures like claws are completely missing and other leg segments are crippled and difficult to recognise). The sternopleura can be identified in several cases, and occasionally some wing hinge tissue can be seen. 5/16 transplanted second leg discs show transdetermination to proximal wing structures and 4/16 show transdetermination to structures of the third antennal segment. Only 2/14 transplanted first leg discs form ectopic notum structures. The legs are crippled and truncated. No differentiated claws are found and only a few occasional sex comb teeth can be seen. 3/3 transplanted third leg discs result in truncated and malformed leg structures (no notum tissue is seen). Mesothoracic leg discs expressing salm[Scer\UAS.cKa] under the control of Scer\GAL4[dpp.blk1] show extra growth in dorsal cells. Wing discs expressing salm[Scer\UAS.cKa] under the control of Scer\GAL4[dpp.blk1] show outgrowths within the central domain (the region that would normally develop into the wing blade). Only a few larvae expressing salm[Scer\UAS.cKa] under the control of Scer\GAL4[bi-omb-Gal4] develop into pharate adults. These animals either have strongly reduced wings or no wings, and instead a supernumerary notum has formed.
Expression of salmScer\UAS.cKa under the control of Scer\GAL4btl.PS in the tracheal system results in autocellular adherens junction (AJ) formation being completely blocked, and all branches are transformed into tubes with cells organised in a paired arrangement and connected via intercellular AJs. Either the cells are not capable of reaching around the lumen or the initial autocellular AJ contacts are not stabilised and zipped up. Single cells expressing salmScer\UAS.cKa under the control of Scer\GAL4btl.B123 in either the dorsal or ventral branches of the tracheal system still form extended autocellular adherens junctions (AJs) (as occurs in wild type). Groups of cells in the dorsal or ganglionic branch expressing salmScer\UAS.cKa under the control of Scer\GAL4btl.B123 are generally inhibited in their capacity to form autocellular AJs and form cell aggregates with intercellular AJs. However, single cells at the periphery of such groups often form autocellular AJs.
When salmScer\UAS.cKa is driven by Scer\GAL4btl.PS the cells of the lateral trunk of the tracheal system adopt a more dorsal identity.
When salmScer\UAS.cKa is driven by Scer\GAL4btl.PS no increase in dorsal trunk cell migration is seen.
Expression of salmScer\UAS.cKa under the control of Scer\GAL4en-e16E results in a high frequency of lateral chordotonal organ arrays containing 3-4 chordotonal organs at 25oC, while at 29oC 3 chordotonal organs per array are mostly seen. A moderate reduction in oenocyte number is also seen.
When expression is driven by Scer\GAL4btl.PS there is no effect on the primary outgrowth of tracheal dorsal branch progenitor cells. Subsequently these cells fail to grow out to the dorsal midline and lack the typical U-turn structure of wild-type dorsal branches. Dorsal anastomosis formation is blocked.
When expression is driven by Scer\GAL4Kr.PM, the number of tracheal primordial cells and the corresponding differentiated tracheal system is reduced. There are no associated defects in the epidermis or peripheral nervous system. When expression is driven by Scer\GAL4salm.TSE, in an otherwise salm mutant background, the dorsal truck branches fuse at high frequency, leading to dorsal trunk fragments which span several tracheal metameric units, constituting a partial rescue of the salm mutant phenotype.
|Phenotype Manifest In|
|NOT Suppressor of|
Expression of salm[Scer\UAS.cKa] under the control of Scer\GAL4 restores the normal muscle fibre structure in dorsal longitudinal indirect flight muscles in vg[null] mutants. Fibrillar transformation of leg muscles is not observed as a result of salm[Scer\UAS.cKa] expression.
The addition of salmScer\UAS.cKa and Scer\GAL4btl.PS to rib1 embryos does not rescue the dorsal trunk migration phenotype.
Coexpression of spis.Scer\UAS and salmScer\UAS.cKa under the control of Scer\GAL4en-e16E results in a chordotonal phenotype identical to that seen when salmScer\UAS.cKa alone is expressed under the control of Scer\GAL4en-e16E. Giant clusters of oenocyte cells are induced dorsally, as is seen when spis.Scer\UAS alone is expressed under the control of Scer\GAL4en-e16E.
|Complementation & Rescue Data|
|Fails to rescue|
|Stocks ( 2 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of Kuhnlein
|Secondary FlyBase IDs|
|References ( 12 )|