Allele Dmel\put⁶²

General Information
Symbol	Dmel\put⁶²	Species	D. melanogaster
Name		FlyBase ID	FBal0051241
Feature type	allele	Associated gene	Dmel\put
Also Known As	punt⁶²

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Simin et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	put¹⁰⁴⁶⁰ (Simin et al., 1998)
Nature of the lesion	Statement Reference Partial excision of the P{PZ} element, with more than 3kb of the P{PZ} sequences remaining. (Simin et al., 1998)
Caused by insertion	P{PZ-Δ.p62}put⁶² (Simin et al., 1998)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| recessive \| heat sensitive (Simin et al., 1998) neuroanatomy defective \| embryonic stage \| heat sensitive (with put¹³⁵) (Serpe and O'Connor, 2006) visible \| recessive (Simin et al., 1998)
Phenotype Manifest In
	abdominal segmental nerve \| heat sensitive (with put¹³⁵) (Serpe and O'Connor, 2006) antenna \| distal \| heat sensitive (Simin et al., 1998) antenna \| heat sensitive (Simin et al., 1998) dorsal closure embryo \| heat sensitive (with put¹³⁵) (Serpe and O'Connor, 2006) dorsal mesothorax \| heat sensitive (Simin et al., 1998) embryonic/first instar larval cuticle \| dorsal \| heat sensitive (Simin et al., 1998) embryonic abdominal segment 1 \| heat sensitive (Simin et al., 1998) embryonic abdominal segment 2 \| heat sensitive (Simin et al., 1998) embryonic abdominal segment 3 \| heat sensitive (Simin et al., 1998) embryonic abdominal segment 4 \| heat sensitive (Simin et al., 1998) embryonic abdominal segment 5 \| heat sensitive (Simin et al., 1998) embryonic abdominal segment 6 \| heat sensitive (Simin et al., 1998) embryonic abdominal segment 7 \| heat sensitive (Simin et al., 1998) embryonic abdominal segment 8 \| heat sensitive (Simin et al., 1998) embryonic abdominal segment 9 \| heat sensitive (Simin et al., 1998) embryonic abdominal segment 10 \| heat sensitive (Simin et al., 1998) embryonic abdominal segment 11 \| heat sensitive (Simin et al., 1998) embryonic epidermis \| heat sensitive (Byars et al., 1999) eye \| heat sensitive (Simin et al., 1998) interommatidial bristle \| heat sensitive (Simin et al., 1998) intersegmental nerve \| heat sensitive (with put¹³⁵) (Serpe and O'Connor, 2006) leg (Penton and Hoffmann, 1996) leg \| distal \| heat sensitive (Simin et al., 1998) leg \| heat sensitive (Simin et al., 1998) ommatidium \| heat sensitive (Simin et al., 1998) sex comb \| heat sensitive (Simin et al., 1998) wing (Penton and Hoffmann, 1996, Simin et al., 1998) wing margin \| ectopic (Penton and Hoffmann, 1996) wing vein (Simin et al., 1998) wing vein \| ectopic (Penton and Hoffmann, 1996)
Detailed Description
	Statement Reference At permissive temperatures of 18-20^oC, put¹³⁵/put⁶² embryos do not show significant axon guidance defects. At 23-25^oC, these embryos show some guidance defects, affecting the intersegmental nerve and the SNa and SNb. At temperatures above 25^oC, embryos of this genotype exhibit gross head involution and dorsal closure defects. (Serpe and O'Connor, 2006) Homozygous embryos maintained at the restrictive temperature (25^oC) have a single, large hole in the dorsal epidermis. (Byars et al., 1999) At 25^oC, 17% of homozygous embryos have a strong dorsal-open phenotype (large holes in the dorsal cuticle), 42% have an intermediate phenotype (the dorsal cuticle is noticeably reduced, and there is increased curvature in the abdominal segments, resulting in a "tail-up" phenotype) and 42% have a weak phenotype (dorsal closure is completed and there are minor defects in the dorsal cuticle). put¹⁰/put⁶² adults are viable, and sometimes show defects in wing venation, with ectopic venation stemming from the posterior crossvein into the second posterior cell. put⁶²/put¹³⁵ flies are inviable at 25^oC and fully viable at 18^oC. put⁶²/put¹³⁵ flies maintained at 18^oC throughout embryogenesis and then shifted to 25^oC show a marked reduction in viability and are grossly deformed. These animals all have notal defects, usually medial notal clefts, and have leg defects in 99% of cases, which include truncations, bifurcations and abnormal twists. Distal pattern elements are usually deleted in at least one limb. Duplications of sex combs are often seen on the forelegs. 97% of these flies have gross eye and antennal defects. The eyes are highly disorganised and have a reduced number of ommatidia and bristles. Antennal defects include duplications and deletions of distal pattern elements. (Simin et al., 1998) Produces a higher number of clones and larger clones than put¹³⁵. Clones induced in 2nd instar larvae that arose in ventral regions or were proximal to the distal tibia have no effect on bristle pattern. However clones close on the extreme dorsal surface of distal leg segments show a loss of dorsal structures, duplication of ventral structures and dorsal bifurcations. Duplicated structures frequently contain wild-type bristles. Clones in adult wings cause reduced wing size, ectopic vein formation, blisters and ectopic wing margin structures. (Penton and Hoffmann, 1996)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	put¹⁰⁴⁶⁰ (Simin et al., 1998)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer
	Selected as: ry^- excision allele. (Simin et al., 1998)
Comments
	Three classes of put allele can be identified with respect to decreasing severity of wing venation phenotypes with put¹⁰: Class I (put¹⁰⁴⁶⁰ = put⁶²) > Class II (put⁵¹ = put⁸⁸ = put¹³⁵) > Class III (put¹⁰ = put²⁴ = put⁹⁷). (Simin et al., 1998)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	punt⁶² (Byars et al., 1999, Horsfield et al., 1998, Simin et al., 1998) put⁶² (Serpe and O'Connor, 2006) put^P62
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Serpe and O'Connor, 2006, Development 133(24): 4969--4979 The metalloprotease tolloid-related and its TGF-beta-like substrate Dawdle regulate Drosophila motoneuron axon guidance. [FBrf0194328] Byars et al., 1999, Development 126(21): 4913--4923 The dorsal-open group gene raw is required for restricted DJNK signaling during closure. [FBrf0111821] Horsfield et al., 1998, Development 125(24): 5069--5078 decapentaplegic is required for arrest in G1 phase during Drosophila eye development. [FBrf0105843] Simin et al., 1998, Genetics 148(2): 801--813 Genetic analysis of punt, a type II Dpp receptor that functions throughout the Drosophila melanogaster life cycle. [FBrf0102010] Penton and Hoffmann, 1996, Nature 382(6587): 162--165 Decapentaplegic restricts the domain of wingless during Drosophila limb patterning. [FBrf0088774]

Allele Dmel\put62

Allele Dmel\put⁶²