Allele Dmel\per^T

General Information
Symbol	Dmel\per^T	Species	D. melanogaster
Name		FlyBase ID	FBal0051296
Feature type	allele	Associated gene	Dmel\per

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Konopka et al., 1994)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation X:2,584,241..2,584,241 evidence=experimental na_change=G2584241A pr_change=G593D\|per-PA reported_na_change=G4800A reported_pr_change=G593D (Hamblen et al., 1998)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Nucleotide substitution: G4800A (numbering system of FBrf0047067). Amino acid replacement: G593D. G593 maps only four amino acids C-terminal to the site of the lesion in per^s. 3bp are deleted from the 3' UTR with respect to the sequence published in FBrf0049579. Two additional bases exist in the per^T sequence, 800 bases upstream of the translation start site, in intron 1. (Hamblen et al., 1998)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective (Andretic et al., 1999) behavior defective \| semidominant (Konopka et al., 1994) circadian rhythm defective (Hamblen et al., 1998)
Phenotype Manifest In

Detailed Description
	Statement Reference per^T mutants show increased responsiveness to initial cocaine 75μg exposure, and weak sensitization (as compared to wild-type) to a second exposure when tested for behavioural responses. These flies do not show sensitization to other cocaine doses. (Andretic et al., 1999) Very fast 16hr clock mutant. (Hamblen et al., 1998) Period-altering mutations. Homozygotes have a 16 hour period, heterozygotes with per⁺ have a 20 hour period and heterozygotes with per^- have a shorter period than with per⁺. The rhythmic mutant can be entrained. At 25^oC individuals exhibit marginal emergence rhythm but at 19^oC periodicity is 17-18 hours. CkIIβ^And combined with per^T exhibits a 1 hour lengthening of the adult periodicities, an additive effect. Combination with so mutation has no effect on rhythms. (Konopka et al., 1994)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference per^T is a suppressor \| partially of circadian rhythm defective phenotype of dco^j3B9/dco^ar (Rothenfluh et al., 2000) per^T is a suppressor \| partially of circadian rhythm defective phenotype of Df(3R)tll-g/dco^ar (Rothenfluh et al., 2000)
NOT Suppressor of
Statement Reference per^T/per[+] is a non-suppressor of circadian rhythm defective \| partially phenotype of tim⁰¹ (Rothenfluh et al., 2000)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference The addition of per^T to dco^j3B9/dco^ar flies suppresses the arrhythmicity phenotype seen in these flies. the addition of per^T/+ has nor effect on the arrhythmia phenotype seen in tim⁰¹ flies. (Rothenfluh et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	per^SLIH (Hamblen et al., 1998)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer
	Induced on: CG2650^T. (Hamblen et al., 1998)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	E331 (Konopka et al., 1994) per^T
Name Synonym	line E331 (Konopka et al., 1994)
Secondary FlyBase IDs

References ( 12 )
Research paper	Stempfl et al., 2002, Genetics 160(2): 571--593 Identification of circadian-clock-regulated enhancers and genes of Drosophila melanogaster by transposon mobilization and luciferase reporting of cyclical gene expression. [FBrf0144900] Rothenfluh et al., 2000, Curr. Biol. 10(21): 1399--1402 Short-period mutations of per affect a double-time-dependent step in the Drosophila circadian clock. [FBrf0131392] Andretic et al., 1999, Science 285(5430): 1066--1068 Requirement of circadian genes for cocaine sensitization in Drosophila. [FBrf0109239] Hamblen et al., 1998, Genetics 149(1): 165--178 Molecular and behavioral analysis of four period mutants in Drosophila melanogaster encompassing extreme short, novel long, and unorthodox arrhythmic types. [FBrf0102841] Goldbeter, 1995, Proc. Biol. Sci. 261(1362): 319--324 A model for circadian oscillations in the Drosophila period protein (PER). [FBrf0083967] Konopka et al., 1994, J. Biol. Rhythms 9(3-4): 189--216 An ultrashort clock mutation at the period locus of Drosophila melanogaster that reveals some new features of the fly's circadian system. [FBrf0080168]
Review	Stanewsky, 2003, J. Neurobiol. 54(1): 111--147 Genetic analysis of the circadian system in Drosophila melanogaster and mammals. [FBrf0155881] Subramanian and Lakhotia, 1999, Curr. Sci. 77(9): 1165--1169 Molecular rhythms that regulate rhythm genes in Drosophila. [FBrf0127339] Hall, 1997, Cell 90(1): 9--12 Circadian pacemakers blowing hot and cold - but they're clocks, not thermometers. [FBrf0094843] Rosato et al., 1997, BioEssays 19(12): 1075--1082 Circadian rhythms: from behaviour to molecules. [FBrf0099853] Dunlap, 1996, A. Rev. Genet. 30: 579--601 Genetic and molecular analysis of circadian rhythms. [FBrf0091051] Hall, 1995, Trends Neurosci. 18(5): 230--240 Tripping along the trail to the molecular mechanisms of biological clocks. [FBrf0082069]

Allele Dmel\perT

Allele Dmel\per^T