Allele Dmel\mew^Scer\UAS.cWa

General Information
Symbol	Dmel\mew^Scer\UAS.cWa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Wehrli	FlyBase ID	FBal0051343
Feature type	allele	Associated gene	Dmel\mew

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Roote and Zusman, 1996, Brabant et al., 1996, Hoang and Chiba, 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Fragment of mew cDNA (nucleotides 315 to 4700, FBrf0064742) is cloned into the pUAST vector. (Brabant et al., 1996) Construct: mew cDNA downstream of Scer\UAS binding sites for Scer\GAL4. (Roote and Zusman, 1996)
Carried in construct	P{UAS-mew.W} (Li et al., 1998, O'Keefe and Thomas, 2001, Boube et al., 2001, Roote and Zusman, 1996, Hoang and Chiba, 1998, Brabant et al., 1996, Tomoyasu et al., 2009, Yang and Terman, 2012)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	dorsal branch primordium, with Scer\GAL4^btl.PS (Boube et al., 2001)
Detailed Description
	Statement Reference In mew^Scer\UAS.cWa, Scer\GAL4^btl.PS embryos, there is abnormal dorsal branch migration and fusion and occasional dorsal trunk breaks. Many dorsal branches fail to fuse with the dorsal branch from the contralateral hemisegment, or fuse with an inappropriate branch. (Boube et al., 2001) Embryos expressing mew^Scer\UAS.cWa under the control of Scer\GAL4^elav-C155 do not show axon defects in the central or peripheral nervous systems. (Hoang and Chiba, 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Scer\GAL4^{14-3-3ε-NP1301}/mew^Scer\UAS.cWa is a suppressor of neuroanatomy defective phenotype of 14-3-3ε^j2B10/14-3-3ε^NP1301 (Yang and Terman, 2012) Scer\GAL4^ap-md544, mew^Scer\UAS.cWa, fng^Scer\UAS.cKa is a suppressor \| partially of visible phenotype of ap^md544/ap^ts78j (O'Keefe and Thomas, 2001)
NOT Suppressor of
Statement Reference Scer\GAL4^elav.PU/mew^Scer\UAS.cWa is a non-suppressor of neuroanatomy defective phenotype of Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} (Yang and Terman, 2012)
Phenotype Manifest In
Suppressor of
Statement Reference Scer\GAL4^{14-3-3ε-NP1301}/mew^Scer\UAS.cWa is a suppressor of intersegmental nerve branch ISNb of A1-7 phenotype of 14-3-3ε^j2B10/14-3-3ε^NP1301 (Yang and Terman, 2012) Scer\GAL4^{14-3-3ε-NP1301}/mew^Scer\UAS.cWa is a suppressor of segmental nerve branch SNa of A1-7 phenotype of 14-3-3ε^j2B10/14-3-3ε^NP1301 (Yang and Terman, 2012) Scer\GAL4^ap-md544, mew^Scer\UAS.cWa, fng^Scer\UAS.cKa is a suppressor \| partially of wing phenotype of ap^md544/ap^ts78j (O'Keefe and Thomas, 2001)
NOT Suppressor of
Statement Reference Scer\GAL4⁶⁸⁴/mew^Scer\UAS.cWa is a non-suppressor of wing \| somatic clone phenotype of if^A7 (Brabant et al., 1996) Scer\GAL4^ap-md544/mew^Scer\UAS.cWa is a non-suppressor of wing phenotype of ap^unspecified (O'Keefe and Thomas, 2001) Scer\GAL4^elav.PU/mew^Scer\UAS.cWa is a non-suppressor of embryonic/larval central nervous system phenotype of Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} (Yang and Terman, 2012)
Other
Statement Reference Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^Scer\UAS.cKa, mew^Scer\UAS.cWa has alula & macrochaeta \| supernumerary phenotype (O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^Scer\UAS.cKa, mew^Scer\UAS.cWa has dorsal row phenotype (O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^Scer\UAS.cKa, mew^Scer\UAS.cWa has sensillum campaniformium \| supernumerary & wing vein L3 \| ventral phenotype (O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^Scer\UAS.cKa, mew^Scer\UAS.cWa has ventral row \| supernumerary phenotype (O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^Scer\UAS.cKa, mew^Scer\UAS.cWa has wing margin phenotype (O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^Scer\UAS.cKa, mew^Scer\UAS.cWa has wing vein phenotype (O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001)
Additional Comments
Genetic Interactions
Statement Reference The ISNb and SNa axon guidance defects seen in 14-3-3ε[j2B10]/14-3-3ε[NP1301] embryos are significantly suppressed by expression of mew[Scer\UAS.cWa] under the control of Scer\GAL4[14-3-3ε-NP1301]. (Yang and Terman, 2012) Expression of mew^Scer\UAS.cWa alone under the control of Scer\GAL4^ap-md544 does not rescue any aspect of the ap^unspecified mutant phenotype. Coexpression of mew^Scer\UAS.cWa and fng^Scer\UAS.cKa under the control of Scer\GAL4^ap-md544 partially rescues the ap^ts78j/ap^md544 wing phenotype; the overall size and shape, apposition of the two surfaces, position of the wing margin and pattern of the wing veins are remarkably normal. However, these wings consist entirely of ventral cell types. Firstly, the rescued wing margins lack the dorsal-specific bristle types and instead have ventral bristle types on both surfaces. Secondly, the wings have many more alula bristles than normal (which in wild-type are derived only from the ventral surface). Thirdly, a mechanosensory campaniform sensillum that is normally located only on the ventral surface of vein L3 is duplicated and present on both surfaces of the rescued wing. Finally, the morphology of the wing veins indicate that they entirely of ventral identity. (O'Keefe and Thomas, 2001) Scer\GAL4⁶⁸⁴ induced expression of mew^Scer\UAS.cWa fails to rescue the blistering phenotype of ventral if^A7 clones. (Brabant et al., 1996)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	mew^Scer\UAS.cWa/Scer\GAL4^hs.PB rescues mew⁴⁹⁸ (Li et al., 1998) Scer\GAL4⁶⁸⁴/mew^Scer\UAS.cWa rescues mew⁴⁹⁸ (Brabant et al., 1996) Scer\GAL4⁶⁸⁴/mew^Scer\UAS.cWa rescues mew^M6 (Brabant et al., 1996) Scer\GAL4^btl.PS/mew^Scer\UAS.cWa rescues mew^M6 (Boube et al., 2001) Scer\GAL4^how-24B, Scer\GAL4^how-24B, mew^Scer\UAS.cWa rescues mew⁴⁹⁸ (Li et al., 1998)
Comments	mew^Scer\UAS.cWa when driven by Scer\GAL4^btl.PS rescues the visceral branch phenotype of mew^M6 embryos. (Boube et al., 2001) Scer\GAL4⁶⁸⁴ induced expression of mew^Scer\UAS.cWa rescues mew^M6 and mew⁴⁹⁸ dorsal clones completely. (Brabant et al., 1996)
Stocks ( 0 )

Notes on Origin
Discoverer	M. Wehrli.

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	mew^Scer\UAS.cWa mew^UAS.cWa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Wehrli (Roote and Zusman, 1996)
Secondary FlyBase IDs

References ( 8 )
Research paper	Yang and Terman, 2012, Neuron 74(1): 108--121 14-3-3ε couples protein kinase A to semaphorin signaling and silences plexin RasGAP-mediated axonal repulsion. [FBrf0218615] Tomoyasu et al., 2009, Curr. Biol. 19(24): 2057--2065 Repeated co-options of exoskeleton formation during wing-to-elytron evolution in beetles. [FBrf0212037] Boube et al., 2001, Genes Dev. 15(12): 1554--1562 Specific tracheal migration is mediated by complementary expression of cell surface proteins. [FBrf0136944] O'Keefe and Thomas, 2001, Development 128(5): 703--710 Drosophila wing development in the absence of dorsal identity. [FBrf0134576] Hoang and Chiba, 1998, J. Neurosci. 18(19): 7847--7855 Genetic analysis on the role of integrin during axon guidance in Drosophila. [FBrf0104450] Li et al., 1998, Development 125(4): 701--711 Requirements for the cytoplasmic domain of the alphaPS1, alphaPS2 and betaPS integrin subunits during Drosophila development. [FBrf0101940] Brabant et al., 1996, Development 122(10): 3307--3317 Distinct spatial and temporal functions for PS integrins during Drosophila wing morphogenesis. [FBrf0090466] Roote and Zusman, 1996, Development 122(6): 1985--1994 Alternatively spliced forms of the Drosophila _PS2 subunit of integrin are sufficient for viability and can replace the function of the _PS1 subunit of integrin in the retina. [FBrf0088875]

Allele Dmel\mewScer\UAS.cWa

Allele Dmel\mew^Scer\UAS.cWa