An additional, ectopic eve
-postive neuron is observed within the nerve cord in 25-41% of hemisegments in ~14 hour-old mutant embryos. In midlos1
embryos, the ectopic neuron is seen in 24-25% of hemisegments; in midlos1
embryos, the ectopic neuron is seen in 30-39% of hemisegments; in midlos1
embryos, the ectopic neuron is seen in 57-65% of hemisegments. Further experiments suggest this is an ectopic RP2 (eRP2) neuron, resulting from the transformation of a wild type neuron termed the 'M-neuron', with an ectopic GMC-1-like cell and a RP2 sib-like cell also present. This extra RP2/sib lineage is formed 2-2.5 hours after the development of the bona fide RP2 lineage.
Mutant phenotype as assayed by Ecol\lacZrp
staining: commissural subset and longitudinal connectives have gaps. Mutant phenotype of lateral chordotonal axons includes: shorter axons, defasciculated axons or dorsally routed axons.