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General Information
Symbol
Dmel\midlos1
Species
D. melanogaster
Name
FlyBase ID
FBal0051368
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:
Reported as a 22 bp deletion resulting in deletion of 7 amino acids at position 321 and a frame shift leading to a stop codon after 28 novel amino acids.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
A 22 bp deletion resulting in a deletion of 7 amino acids at position 321 and a frame shift leading to a STOP codon at amino acid position 350. Thus the resulting protein is predicted to have 28 amino acids that are different from wild type, in addition to the truncation.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
midlos1/midlos1 embryos show loss of lateral transverse muscles.
An additional, ectopic eve-postive neuron is observed within the nerve cord in 25-41% of hemisegments in ~14 hour-old mutant embryos. In midlos1/mid2 embryos, the ectopic neuron is seen in 24-25% of hemisegments; in midlos1/mid1 embryos, the ectopic neuron is seen in 30-39% of hemisegments; in midlos1/Df(2L)Exel6012 embryos, the ectopic neuron is seen in 57-65% of hemisegments. Further experiments suggest this is an ectopic RP2 (eRP2) neuron, resulting from the transformation of a wild type neuron termed the 'M-neuron', with an ectopic GMC-1-like cell and a RP2 sib-like cell also present. This extra RP2/sib lineage is formed 2-2.5 hours after the development of the bona fide RP2 lineage.
Mutant phenotype as assayed by Ecol\lacZrp staining: commissural subset and longitudinal connectives have gaps. Mutant phenotype of lateral chordotonal axons includes: shorter axons, defasciculated axons or dorsally routed axons.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference
In midlos1, insc22 mutants, two ectopic RP2s are observed. In midlos1, numb796 mutants, two ectopic RP2 sib cells are observed with complete penetrance. The ectopic RP2 neuron seen in midlos1 mutants is not observed in any animals that are also mutant for Df(2L)ED773 (which deletes nub and pdm2), and is missing in 75-96% of the hemisegments in midlos1, wgl-17 double mutants.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Not rescued by
Comments
Expression of midScer\UAS.cGa using Scer\GAL4wg.PM, Scer\GAL4sca.PU, Scer\GAL4ftz.ng or Scer\GAL4eve.RKK, but not Scer\GAL4en-e16E, significantly suppresses the formation of the extra RP2 lineage in midlos1 mutants.
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (4)