Open Close
General Information
Symbol
Dmel\hhUAS.cIa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Ingham
FlyBase ID
FBal0051518
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-hh, UAShh
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of a cDNA fragment including the entire hh open reading frame.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
embryonic abdominal segment 1 & cuticle, with Scer\GAL4prd.RG1
embryonic abdominal segment 1 & denticle belt, with Scer\GAL4prd.RG1
embryonic abdominal segment 3 & denticle belt, with Scer\GAL4prd.RG1
embryonic abdominal segment 3 & denticle belt | supernumerary, with Scer\GAL4prd.RG1
embryonic abdominal segment 5 & denticle belt, with Scer\GAL4prd.RG1
embryonic abdominal segment 5 & denticle belt | supernumerary, with Scer\GAL4prd.RG1
embryonic abdominal segment 7 & denticle belt, with Scer\GAL4prd.RG1
embryonic abdominal segment 7 & denticle belt | supernumerary, with Scer\GAL4prd.RG1
embryonic thoracic segment & cuticle, with Scer\GAL4prd.RG1
embryonic thoracic segment & denticle belt, with Scer\GAL4prd.RG1
Detailed Description
Statement
Reference
Expression of hhScer\UAS.cIa under the control of Scer\GAL4vg.int2.1 results in extensive overproliferation of anterior compartment cells in the wing disc.
Flies overexpressing hhScer\UAS.cIa under the control of Scer\GAL4Hml.Δ in the hemocytes develop normally, with no obvious defects after they hatch. Expression of hhScer\UAS.cIa does not protect against infection with group B streptococcus.
Expression of hhScer\UAS.cIa, under the control of two copies of Scer\GAL4sim.PS, causes cell death in embryos. Expression of hhScer\UAS.cIa, under the control of Scer\GAL4sca-537.4, abolishes the formation of the anterior commissure, but not of the posterior commissure. The differentiation of midline glia and MP1 neurons is also affected. hhScer\UAS.cIa expression in all midline cells from late stage 9, driven by Scer\GAL4sim.PS, results in a severe reduction in cell number. The few remaining axons of the surviving midline cells suggest that the UMI and VUM interneurons are still able to differentiate. When cells expressing hhScer\UAS.cIa, under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16, are transplanted close to the midline, the differentiation of MP1 neurons is disrupted. The resulting lack of MP1 axons causes a gap in the MP1 axon fascicle. In some embryos, axons project randomly across the longitudinal tracts. The number of midline cells in the affected neuromeres is not reduced, although the midline glia are occasionally absent or pushed out of the CNS. The axons of the VUM interneurons defasciculate slightly. All other midline cell lineages develop normally.
Overexpression of hhScer\UAS.cIa under the control of Scer\GAL4lz-gal4 behind the morphogenetic furrow results in mildly disordered bristles and ommatidial packing. The pupal retinas show mild defects near one edge, with occasional loss or excess cone cells (4%), mis-oriented of incompletely wrapped primary cells (44%) and excess lattice cells (55%). Photoreceptor differentiation, assessed by counting rhabdomeres in pupal retinas, is unaffected in these mutants.
Expression of hhScer\UAS.cIa under the control of Scer\GAL4dpp.3KK results in partial loss of wing vein L3.
Expression of hhScer\UAS.cIa under the control of Scer\GAL4pb.PJ does not affect labial palp development.
The cuticles formed by hhScer\UAS.cIa; Scer\GAL469B embryos have reduced denticle diversity: rows 3-5 adopt row 2 identity (there is often an extra row 2 in addition to these), while row 6 adopts row 1 identity.
When hhScer\UAS.cIa is driven by Scer\GAL469B in the embryo a mirror image duplication of anterior denticles is seen in the abdominal area. The amount of naked cuticle seen is reduced compared to wild-type.
Expression of hhScer\UAS.cIa under the control of Scer\GAL4h-1J3 results in germ cell migration defects in the embryo. These defects are evident just after the germ cells leave the posterior midgut; instead of migrating dorsally towards the overlying mesoderm, some germ cells in stage 10-11 embryos remain associated with the posterior midgut or migrate in the opposite direction towards the ventral side of the embryo. In stage 13 embryos, germ cells are seen at a variety of abnormal positions in the posterior ectoderm in addition to the normal cluster of germ cells in the mesoderm near the posterior of the embryo. Expression of hhScer\UAS.cIa under the control of Scer\GAL4elav.PLu results in abnormal germ cell migration patterns in the embryo. Two clusters of germ cells on either side of the ventral midline can be seen in these embryos at stage 13, but in contrast to wild-type embryos the cells within the clusters are not tightly associated with each other and some are dispersed at distant sites in the posterior mesoderm. In addition, some germ cells are typically found near the ventral midline in the region just underlying the central nervous system. Expression of hhScer\UAS.cIa under the control of Scer\GAL4twi.PG results in germ cell migration defects in the embryo. In these embryos at stage 12-13, germ cells are found in several small clusters of 3-6 cells scattered in different segments along the anterior-posterior axis. In stage 14-15 embryos, many of the germ cells are clustered near the very posterior of the embryo. Expression of hhScer\UAS.cIa under the control of Scer\GAL4how-24B results in germ cells congregating in the region of the mesoderm just posterior to the midgut invagination (the site of ectopic hh expression) in stage 11 embryos.
Embryos in which hhScer\UAS.cIa is expressed under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 have a deranged overall morphology, but the tracheal cells are capable of forming all the branches except the dorsal branch. The morphology of the branches is slightly affected but primary patterning proceeds normally. Embryos in which hhScer\UAS.cIa is expressed under the control of Scer\GAL4btl.PS have almost normal branching of the primary and secondary tracheal branches, with occasional defects in the dorsal branch and lateral trunk posterior. Excess terminal cells and excess terminal branching are seen in the dorsal branch.
Embryos expressing hhScer\UAS.cIa under the control of Scer\GAL4prd.RG1 show mutant phenotypes in every other segment. Thoracic and the first abdominal segment are principally covered by naked cuticle, while abdominal segments 3, 5 and 7 show mirror image duplication of their denticle belts, showing duplication of denticle rows 1 and 2.
Ectopic expression of hhScer\UAS.cIa by Scer\GAL4dpp.blk1 causes severely disrupted eye-antennal disc morphology.
When expression is driven by Scer\GAL4en-e16E the denticle belts are affected such that there is an excess of small denticles, of type row 2-4, at the expense of rows 5 and 6.
Flies expressing hhScer\UAS.cIa under the control of Scer\GAL4en-e16E show anterior displacement of wing vein 3 and a more anterior restriction of the double row bristles, leading to an expansion of the intervein 3-4 region.
Scer\GAL430A driven expression causes dramatic respecification of the pupal wing anterior compartment, costa is eliminated and triple row bristles are present on most of the anterior wing margin are replaced by double row bristles. Pupae also exhibit duplication of notal structures. Scer\GAL434B driven expression causes suppression of the costa and overgrowth in the anterior wing compartment resulting in a bulge in the anterior margin and an increase in the distance between veins II and III.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Scer\GAL4en-e16E, hhUAS.cIa has visible phenotype, suppressible by fu1
Phenotype Manifest In
Suppressed by
Statement
Reference
Scer\GAL4prd.RG1, hhUAS.cIa has embryonic abdominal segment 3 & denticle belt | supernumerary phenotype, suppressible by ci94
Scer\GAL4prd.RG1, hhUAS.cIa has embryonic abdominal segment 5 & denticle belt | supernumerary phenotype, suppressible by ci94
Scer\GAL4prd.RG1, hhUAS.cIa has embryonic abdominal segment 7 & denticle belt | supernumerary phenotype, suppressible by ci94
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference
Expression of hhScer\UAS.cIa under the control of Scer\GAL4prd.RG1 does not restore naked cuticle in dlpA187 embryos derived from dlpA187 female germline clones.
Expression of hhScer\UAS.cIa, under the control of Scer\GAL4unspecified in dispS037707 mutant embryos rescues some type 1 cuticle, but no type 2 or type 1 cuticle is formed.
Expression of hhScer\UAS.cIa with Scer\GAL4en-e16E in dispS037707 germ-line clone embryos doesn't rescue naked cuticle formation, but does increase denticle diversity in the ventral cuticle: row 3,4 and 5 type denticles are all present. Expression of hhScer\UAS.cIa with Scer\GAL4en-e16E in ttv00681b germ-line clone embryos doesn't rescue naked cuticle formation, but does increase denticle diversity in the ventral cuticle: denticles corresponding to row 2,3,4, and 5 types are all present.
hhScer\UAS.cIa; Scer\GAL4prd.RG1 fails to rescue metameric furrow formation in Df(2R)enE; wgl-17 double homozygous embryos.
When wgScer\UAS.cKa or hhScer\UAS.cIa is driven by Scer\GAL4prd.RG1 in sll7E18 animals suppression of the segment polarity phenotype is seen in those domains that express Scer\GAL4prd.RG1.
When hhScer\UAS.cIa is expressed under the control of Scer\GAL4prd.RG1 in a an embryo that is zygotically and maternally mutant for frc00073, naked cuticle is restored in a typical pair-rule pattern.
The mirror image duplication of denticle belts seen in abdominal segments 3, 5 and 7 of embryos expressing hhScer\UAS.cIa under the control of Scer\GAL4prd.RG1 is rescued if they are also mutant for ci94. The denticle types present (5 and 6) in tsh8 mutant embryos are unchanged if they are also expressing hhScer\UAS.cIa under the control of Scer\GAL4prd.RG1, although in some segments the denticle belts are reduced and the shape of the denticle belt is modified.
When expression is driven in a wgl-17, wgScer\UAS.cLa, Scer\GAL4en-e16E background the extent of naked cuticle anterior to the Scer\GAL4en-e16E-driven wg source is narrower than in wgl-17, wgScer\UAS.cLa, Scer\GAL4en-e16E without the hhScer\UAS.cIa.
The wing phenotype seen in flies expressing hhScer\UAS.cIa under the control of Scer\GAL4en-e16E is completely suppressed by fu1.
Expression of hhScer\UAS.cIa under the control of Scer\GAL4prd.RG1 in sgl08310 embryos induces naked cuticle at 16oC and 25oC.
Scer\GAL4e22c-mediated expression in ptcG12 mutants changes the denticle belt morphology. Scer\GAL4h-1J3-mediated expression in smo3 mutants at 18oC does not modify the smo phenotype.
Scer\GAL4arm.PS mediated expression of both wgScer\UAS.cLa and hhScer\UAS.cIa in wgl-17 Df(2R)enE embryos causes naked cuticle to form in the place of the T1 beard.
Scer\GAL4h-1J3 induced expression fails to rescue naked cuticle phenotype of smo- embryos.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Expression of hhScer\UAS.cIa under the control of Scer\GAL4vg.PM completely rescues the lethality seen in homozygous hhts2 mutant flies grown at the restrictive temperature.
Naked cuticle formation in hh15/hhAC embryonic cuticle is rescued by hhScer\UAS.cIa; Scer\GAL469B, but the denticle belts formed consist of only row 1 2 and 3 type denticles.
hhScer\UAS.cIa partially rescues the hh8 tracheal defects when expressed under the control of Scer\GAL4btl.PS. In particular, rescue of the dorsal trunk, visceral branches and some lateral trunk anterior branches is seen. The ectodermal defects are not rescued in these embryos.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
hhScer\UAS.cIa
hhUAS.cIa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Ingham
Secondary FlyBase IDs
    References (44)