FlyBase Allele Report: Dmel\dsh[UAS.cNa]

General Information

Symbol

Dmel\dsh^UAS.cNa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Neumann

FlyBase ID

FBal0051655

Feature type

allele

Associated gene

Dmel\dsh

Associated Insertion(s)

Carried in Construct

P{UAS-dsh.N}

Also Known As

UAS-Dsh

Key Links

Transgenic product class

cDNA

(Neumann and Cohen, 1996)

wild_type

(Neumann and Cohen, 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Neumann and Cohen, 1996)

wild_type

(Neumann and Cohen, 1996)

Mutagen

in vitro construct

(Neumann and Cohen, 1997, Neumann and Cohen, 1996)

Progenitor genotype

Carried in construct

P{UAS-dsh.N}

Cytology

Description

UASt regulatory sequences drive expression of a full-length dsh cDNA.

(Neumann and Cohen, 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsh

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity, with Scer\GAL4^hs.2sev

(Klein et al., 2006)

abnormal planar polarity, with Scer\GAL4^hs.2sev

(Hermle et al., 2010, Strutt et al., 1997)

Phenotype Manifest In

eye photoreceptor cell, with Scer\GAL4^hs.2sev

(Klein et al., 2006)

ommatidium, with Scer\GAL4^hs.2sev

(Hermle et al., 2010, Klein et al., 2006, Strutt et al., 1997)

wing disc, with Scer\GAL4^ptc-559.1

(Mohit et al., 2006)

wing margin bristle | ectopic, with Scer\GAL4^vg.PM

(Bajpai et al., 2004)

Detailed Description

Statement

Reference

Mutant wing disc clones expressing dsh^Scer\UAS.cNa under the control of Scer\GAL4^tub.PU do not show neoplastic transformation.

(Khan et al., 2013)

Expression of dsh^Scer\UAS.cNa under the control of Scer\GAL4^hs.2sev results in planar cell polarity defects in the eye.

(Hermle et al., 2010)

Somatic clones overexpressing dsh^Scer\UAS.cNa under the control of Scer\GAL4^e22c exhibit an increase in wg signaling and apical cell constrictions. They separate from their neighbours and display smooth clone borders.

(Jaiswal et al., 2006)

Expression of dsh^Scer\UAS.cNa in the eye, under the control of Scer\GAL4^hs.2sev, causes planar cell polarity defects and a loss of photoreceptors.

(Klein et al., 2006)

Expression of dsh^Scer\UAS.cNa, under the control of Scer\GAL4^ptc-559.1, results on notum-to-wing pouch transformations in the dorsal mesothoracic disc.

(Mohit et al., 2006)

The wings of dsh^Scer\UAS.cNa; Scer\GAL4^vg.PM flies have tufts of ectopic wing margin bristles.

(Bajpai et al., 2004)

Causes a tissue polarity defect when expressed in a subset of photoreceptors in the developing eye using Scer\GAL4^hs.2sev; ommatidia frequently show abnormal rotation and chirality.

(Strutt et al., 1997)

Expression driven by P{GawB}Bx^MS1096 causes overgrowth of the dorsal hinge and densely packed wing margin bristles over the wing blade surfaces.

(Neumann and Cohen, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, dsh^UAS.cNa has abnormal planar polarity phenotype, suppressible by Rho1^k07903

(Strutt et al., 1997)

Scer\GAL4^hs.2sev, dsh^UAS.cNa has abnormal planar polarity phenotype, suppressible by Rho1^72R

(Strutt et al., 1997)

Scer\GAL4^hs.2sev, dsh^UAS.cNa has abnormal planar polarity phenotype, suppressible by bsk²

(Strutt et al., 1997)

NOT suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, dsh^UAS.cNa has abnormal planar polarity phenotype, non-suppressible by ATP6AP2^EY03616/CG8444[+]

(Hermle et al., 2010)

Scer\GAL4^hs.2sev, dsh^UAS.cNa has abnormal planar polarity phenotype, non-suppressible by rl¹⁰

(Strutt et al., 1997)

Other

Statement

Reference

Scer\GAL4^Tub.PU, dsh^UAS.cNa, l(2)gl⁴ has FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone phenotype

(Khan et al., 2013)

Scer\GAL4^Tub.PU, dsh^UAS.cNa, l(2)gl⁴ has neoplasia | somatic clone phenotype

(Khan et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, dsh^UAS.cNa has wing disc phenotype, enhanceable by Cyp310a1^RNAi.Sym.UAS, Scer\GAL4^ptc-559.1

(Mohit et al., 2006)

Scer\GAL4^ptc-559.1, dsh^UAS.cNa has wing disc phenotype, enhanceable by Scer\GAL4^ptc-559.1/peg^RNAi.Sym.UAS

(Mohit et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, dsh^UAS.cNa has eye photoreceptor cell phenotype, suppressible by dco^j3B9/dco[+]

(Klein et al., 2006)

Scer\GAL4^ptc-559.1, dsh^UAS.cNa has wing disc phenotype, suppressible by Cyp310a1^UAS.cMa, Scer\GAL4^ptc-559.1

(Mohit et al., 2006)

Scer\GAL4^vg.PM, dsh^UAS.cNa has wing margin bristle | ectopic phenotype, suppressible | partially by tws[+]/tws^j11C8

(Bajpai et al., 2004)

Scer\GAL4^hs.2sev, dsh^UAS.cNa has ommatidium phenotype, suppressible by Rho1^k07903

(Strutt et al., 1997)

Scer\GAL4^hs.2sev, dsh^UAS.cNa has ommatidium phenotype, suppressible by Rho1^72R

(Strutt et al., 1997)

Scer\GAL4^hs.2sev, dsh^UAS.cNa has ommatidium phenotype, suppressible by bsk²

(Strutt et al., 1997)

NOT suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, dsh^UAS.cNa has ommatidium phenotype, non-suppressible by ATP6AP2^EY03616/CG8444[+]

(Hermle et al., 2010)

Suppressor of

Statement

Reference

dsh^UAS.cNa/Scer\GAL4^vg.PM is a suppressor | partially of wing phenotype of wg^spd-fg/wg^spd-hL2

(Neumann and Cohen, 1996)

Other

Statement

Reference

Scer\GAL4^Tub.PU, dsh^UAS.cNa, l(2)gl⁴ has wing disc | distal | somatic clone phenotype

(Khan et al., 2013)

Scer\GAL4^Tub.PU, dsh^UAS.cNa, l(2)gl⁴ has wing disc | proximal | somatic clone phenotype

(Khan et al., 2013)

Scer\GAL4^hs.2sev, dsh^UAS.cNa, fz¹ has ommatidium phenotype

(Strutt et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of dsh^Scer\UAS.cNa under the control of Scer\GAL4^tub.PU in l(2)gl⁴ mutant wing disc mutant clones results in overproliferation and neoplastic transformation in the proximal domain. Clones in the distal domain are eliminated, even when the growth temperature is elevated.

(Khan et al., 2013)

The planar cell polarity defects in the eye which are caused by expression of dsh^Scer\UAS.cNa under the control of Scer\GAL4^hs.2sev are not suppressed by VhaM8.9^EY03616/+.

(Hermle et al., 2010)

Expression of dsh^Scer\UAS.cNa under the control of Scer\GAL4^hs.2sev in a dco^j3B9/+ background results in a strong suppression of the loss of photoreceptor cell phenotype seen when the transgene is expressed in a wild-type background.

(Klein et al., 2006)

Simultaneous expression of Cyp310a1^{dsRNA.Sym.Scer\UAS} or CG17278^{dsRNA.Sym.Scer\UAS} to create Cyp310a1 or CG17278 dsRNA results in an enhancement of the notum-to-wing pouch transformation within the dorsal mesothoracic disc in flies expressing dsh^Scer\UAS.cNa, under the control of Scer\GAL4^ptc-559.1. Conversely, expression of Cyp310a1^Scer\UAS.cMa or CG17278^Scer\UAS.cMa suppresses the Scer\GAL4^ptc-559.1>dsh^Scer\UAS.cNa phenotype.

(Mohit et al., 2006)

Ectopic wing margin bristles on the wings of dsh^Scer\UAS.cNa; Scer\GAL4^vg.PM animals are almost completely suppressed by tws^j11C8/+.

(Bajpai et al., 2004)

The tissue polarity phenotype caused by expression of dsh^Scer\UAS.cNa using Scer\GAL4^hs.2sev is dominantly suppressed by Rho1^k07903, Rho1^72R and bsk² but is not affected by rl¹⁰.

(Strutt et al., 1997)

wg^spd-fg/wg^spd-hL2 transheterozygous wing phenotype can be partially rescued by Scer\GAL4^vg.PM induced expression of dsh^Scer\UAS.cNa, rescue of the anterior wing margin and the posterior wing blade between vein 5 and the posterior margin.

(Neumann and Cohen, 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dsh^Scer\UAS.cNa

dsh^UAS.cNa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Neumann

(Neumann and Cohen, 1996)

Secondary FlyBase IDs

References (16)

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