wing | proximal, with Scer\GAL430A
Overexpression of ciScer\UAS.cAa under the control of Scer\GAL4pb.PJ leads to loss and disorganization of the pseudotracheal rows in the anterior compartment of the labial palps. This effect is less severe when flies are maintained at 22oC than when maintained at 28oC.
ciScer\UAS.cAa (when driven by Scer\GAL469B) induces a reduction in the number of denticles in a variable fashion. Enlargements of thoracic segments are seen which are covered in naked cuticle. This effect is penetrant when tested at 18oC 22oC and 29oC. At 29oC variable suppression of the first abdominal belt is seen, and sometimes all denticle belts of the abdomen.
Expression of ciScer\UAS.cAa under the control of Scer\GAL4bs-1348 results in a mild wing vein phenotype.
ciScer\UAS.cAa driven by Scer\GAL4cad-em459 produces very large anal plates, about twice the wild-type size. Anal plate bristles are also seen on the hindgut.
Animals expressing ciScer\UAS.cAa under the control of Scer\GAL4αTub84B.PP in an hhts2 background that have been raised at 28oC after hatching contain S phase lamina neuron precursors (LPCs) in the lamina.
Scer\GAL430A induced expression causes lethality during late larval or pupal stages, pharate adults exhibit outgrowths from the proximal wing region.
Scer\GAL4bs-1348, ciUAS.cAa has visible phenotype, suppressible by Su(hh)IIII
Scer\GAL4bs-1348, ciUAS.cAa has visible phenotype, suppressible by Su(hh)IIIIII
Scer\GAL4bs-1348, ciUAS.cAa has visible phenotype, suppressible | partially by Su(hh)IVIV
Scer\GAL4bs-1348, ciUAS.cAa has visible phenotype, non-suppressible by Su(hh)II
Scer\GAL4pb.PJ, ciUAS.cAa has pseudotrachea phenotype, enhanceable by pb5/pb[+]
Scer\GAL469B, ciUAS.cAa has denticle phenotype, enhanceable by wgl-17
Scer\GAL469B, ciUAS.cAa has embryonic/first instar larval cuticle phenotype, enhanceable by wgl-17
ciUAS.cAa has embryonic epidermis phenotype, enhanceable by wgl-17/Scer\GAL469B
Scer\GAL4pb.PJ, ciUAS.cAa has pseudotrachea phenotype, suppressible by pbUAS.A/Scer\GAL4pb.PJ
Scer\GAL4bs-1348, ciUAS.cAa has wing vein phenotype, suppressible by Su(hh)IIII
Scer\GAL4bs-1348, ciUAS.cAa has wing vein phenotype, suppressible by Su(hh)IIIIII
Scer\GAL4bs-1348, ciUAS.cAa has wing vein phenotype, suppressible | partially by Su(hh)IVIV
Scer\GAL4bs-1348, ciUAS.cAa has wing vein phenotype, non-suppressible by Su(hh)II
Scer\GAL4αTub84B.PP, ciUAS.cAa, tsh8 has denticle belt phenotype
Scer\GAL4αTub84B.PP, ciUAS.cAa, tsh8 has embryonic/first instar larval cuticle phenotype
Scer\GAL4αTub84B.PP, ciUAS.cAa, wgl-17 has embryonic/first instar larval cuticle phenotype
Scer\GAL4wg.PM, ciUAS.cAa, wgl-17 has embryonic/first instar larval cuticle phenotype
Scer\GAL4αTub84B.PP, arm2, ciUAS.cAa has embryonic/first instar larval cuticle | germline clone phenotype
Scer\GAL4αTub84B.PP, arm2, ciUAS.cAa has mesothoracic ventral denticle belt | germline clone phenotype
Scer\GAL4αTub84B.PP, arm2, ciUAS.cAa has metathoracic ventral denticle belt | germline clone phenotype
Scer\GAL4αTub84B.PP, arm2, ciUAS.cAa has prothoracic ventral denticle belt | germline clone phenotype
Scer\GAL4αTub84B.PP, ciUAS.cAa, wg2 has embryonic/first instar larval cuticle | germline clone phenotype
Scer\GAL4αTub84B.PP, ciUAS.cAa, wg2 has mesothoracic ventral denticle belt | germline clone phenotype
Scer\GAL4αTub84B.PP, ciUAS.cAa, wg2 has metathoracic ventral denticle belt | germline clone phenotype
Expression of ciScer\UAS.cAa under the control of Scer\GAL4pb.PJ in pb5/+ heterozygotes enhances the reduced pseudotracheal row phenotype of ciScer\UAS.cAa single mutants. When pbScer\UAS.A is overexpressed along with ciScer\UAS.cAa, both under the control of Scer\GAL4pb.PJ, the pseudotracheal row phenotype is suppressed and labial palps appear almost the same as wild type.
When ciScer\UAS.cAa (driven by Scer\GAL469B) is expressed in a wgl-17 background the naked cell fate is induced in the thorax and the most anterior part of the abdomen. Unpigmented denticles are seen in the posterior trunk. However, when ciScer\UAS.cAa is driven by Scer\GAL4αTub84B.PP in a wgl-17 background, naked cuticle is formed in the thorax but denticles are present throughout the abdomen. If ciScer\UAS.cAa is driven by Scer\GAL4wg.PM in a wgl-17 background naked cuticle is produced in a cell autonomous manner. The addition of ciScer\UAS.cAa (driven by Scer\GAL4αTub84B.PP) to arm2 germ-line clones, can suppress denticle belt formation in the thorax of mutant embryos but not in the abdomen. The addition of ciScer\UAS.cAa (driven by Scer\GAL4αTub84B.PP) to tsh8 mutant embryos, can suppress denticle belt formation in the thorax and the abdomen.
Su(hh)IIII or Su(hh)IIIIII rescue the wing vein patterning of animals expressing ciScer\UAS.cAa under the control of Scer\GAL4bs-1348 to almost wild type. Su(hh)IVIV rescues to almost wild type the wing vein patterning in the anterior compartment of the wing of animals expressing ciScer\UAS.cAa under the control of Scer\GAL4bs-1348.