FlyBase Allele Report: Dmel\ci[UAS.cAa]

General Information

Symbol

Dmel\ci^UAS.cAa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Alexandre

FlyBase ID

FBal0051700

Feature type

allele

Associated gene

Dmel\ci

Associated Insertion(s)

Carried in Construct

P{UAS-ci.A}

Also Known As

UAS-ci

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Alexandre et al., 1996)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-ci.A}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of a full length ci cDNA.

(Alexandre et al., 1996)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^30A

(Haines and van Den Heuvel, 2000, Alexandre et al., 1996)

lethal - all die during embryonic stage, with Scer\GAL4^en-e16E

(Alexandre et al., 1996)

some die during embryonic stage, with Scer\GAL4^en-e16E

(Alexandre et al., 1996)

visible, with Scer\GAL4^bs-1348

(Haines and van Den Heuvel, 2000)

visible, with Scer\GAL4^cad-em459

(Moreno and Morata, 1999)

Phenotype Manifest In

adult hindgut, with Scer\GAL4^cad-em459

(Moreno and Morata, 1999)

cercus, with Scer\GAL4^cad-em459

(Moreno and Morata, 1999)

denticle, with Scer\GAL4^69B

(Angelats et al., 2002)

embryonic/first instar larval cuticle, with Scer\GAL4^69B

(Angelats et al., 2002)

embryonic epidermis, with Scer\GAL4^69B

(Angelats et al., 2002)

larval abdominal segment, with Scer\GAL4^69B

(Angelats et al., 2002)

larval thoracic segment, with Scer\GAL4^69B

(Angelats et al., 2002)

pseudotrachea, with Scer\GAL4^pb.PJ

(Joulia et al., 2005)

wing | proximal, with Scer\GAL4^30A

(Alexandre et al., 1996)

wing vein, with Scer\GAL4^bs-1348

(Haines and van Den Heuvel, 2000)

Detailed Description

Statement

Reference

Overexpression of ci^Scer\UAS.cAa under the control of Scer\GAL4^pb.PJ leads to loss and disorganization of the pseudotracheal rows in the anterior compartment of the labial palps. This effect is less severe when flies are maintained at 22^oC than when maintained at 28^oC.

(Joulia et al., 2005)

ci^Scer\UAS.cAa (when driven by Scer\GAL4^69B) induces a reduction in the number of denticles in a variable fashion. Enlargements of thoracic segments are seen which are covered in naked cuticle. This effect is penetrant when tested at 18^oC 22^oC and 29^oC. At 29^oC variable suppression of the first abdominal belt is seen, and sometimes all denticle belts of the abdomen.

(Angelats et al., 2002)

Expression of ci^Scer\UAS.cAa under the control of Scer\GAL4^bs-1348 results in a mild wing vein phenotype.

(Haines and van Den Heuvel, 2000)

ci^Scer\UAS.cAa driven by Scer\GAL4^cad-em459 produces very large anal plates, about twice the wild-type size. Anal plate bristles are also seen on the hindgut.

(Moreno and Morata, 1999)

Animals expressing ci^Scer\UAS.cAa under the control of Scer\GAL4^αTub84B.PP in an hh^ts2 background that have been raised at 28^oC after hatching contain S phase lamina neuron precursors (LPCs) in the lamina.

(Huang and Kunes, 1998)

Scer\GAL4^30A induced expression causes lethality during late larval or pupal stages, pharate adults exhibit outgrowths from the proximal wing region.

(Alexandre et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^bs-1348, ci^UAS.cAa has visible phenotype, suppressible by Su(hh)II^II

(Haines and van Den Heuvel, 2000)

Scer\GAL4^bs-1348, ci^UAS.cAa has visible phenotype, suppressible by Su(hh)III^III

(Haines and van Den Heuvel, 2000)

Scer\GAL4^bs-1348, ci^UAS.cAa has visible phenotype, suppressible | partially by Su(hh)IV^IV

(Haines and van Den Heuvel, 2000)

NOT suppressed by

Statement

Reference

Scer\GAL4^bs-1348, ci^UAS.cAa has visible phenotype, non-suppressible by Su(hh)I^I

(Haines and van Den Heuvel, 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^pb.PJ, ci^UAS.cAa has pseudotrachea phenotype, enhanceable by pb⁵/pb[+]

(Joulia et al., 2005)

Scer\GAL4^69B, ci^UAS.cAa has denticle phenotype, enhanceable by wg^l-17

(Angelats et al., 2002)

Scer\GAL4^69B, ci^UAS.cAa has embryonic/first instar larval cuticle phenotype, enhanceable by wg^l-17

(Angelats et al., 2002)

ci^UAS.cAa has embryonic epidermis phenotype, enhanceable by wg^l-17/Scer\GAL4^69B

(Angelats et al., 2002)

Suppressed by

Statement

Reference

Scer\GAL4^pb.PJ, ci^UAS.cAa has pseudotrachea phenotype, suppressible by pb^UAS.A/Scer\GAL4^pb.PJ

(Joulia et al., 2005)

Scer\GAL4^bs-1348, ci^UAS.cAa has wing vein phenotype, suppressible by Su(hh)II^II

(Haines and van Den Heuvel, 2000)

Scer\GAL4^bs-1348, ci^UAS.cAa has wing vein phenotype, suppressible by Su(hh)III^III

(Haines and van Den Heuvel, 2000)

Scer\GAL4^bs-1348, ci^UAS.cAa has wing vein phenotype, suppressible | partially by Su(hh)IV^IV

(Haines and van Den Heuvel, 2000)

NOT suppressed by

Statement

Reference

Scer\GAL4^bs-1348, ci^UAS.cAa has wing vein phenotype, non-suppressible by Su(hh)I^I

(Haines and van Den Heuvel, 2000)

Other

Statement

Reference

Scer\GAL4^αTub84B.PP, ci^UAS.cAa, tsh⁸ has denticle belt phenotype

(Angelats et al., 2002)

Scer\GAL4^αTub84B.PP, ci^UAS.cAa, tsh⁸ has embryonic/first instar larval cuticle phenotype

(Angelats et al., 2002)

Scer\GAL4^αTub84B.PP, ci^UAS.cAa, wg^l-17 has embryonic/first instar larval cuticle phenotype

(Angelats et al., 2002)

Scer\GAL4^wg.PM, ci^UAS.cAa, wg^l-17 has embryonic/first instar larval cuticle phenotype

(Angelats et al., 2002)

Scer\GAL4^αTub84B.PP, arm², ci^UAS.cAa has embryonic/first instar larval cuticle | germline clone phenotype

(Angelats et al., 2002)

Scer\GAL4^αTub84B.PP, arm², ci^UAS.cAa has mesothoracic ventral denticle belt | germline clone phenotype

(Angelats et al., 2002)

Scer\GAL4^αTub84B.PP, arm², ci^UAS.cAa has metathoracic ventral denticle belt | germline clone phenotype

(Angelats et al., 2002)

Scer\GAL4^αTub84B.PP, arm², ci^UAS.cAa has prothoracic ventral denticle belt | germline clone phenotype

(Angelats et al., 2002)

Scer\GAL4^αTub84B.PP, ci^UAS.cAa, wg² has embryonic/first instar larval cuticle | germline clone phenotype

(Angelats et al., 2002)

Scer\GAL4^αTub84B.PP, ci^UAS.cAa, wg² has mesothoracic ventral denticle belt | germline clone phenotype

(Angelats et al., 2002)

Scer\GAL4^αTub84B.PP, ci^UAS.cAa, wg² has metathoracic ventral denticle belt | germline clone phenotype

(Angelats et al., 2002)

Scer\GAL4^αTub84B.PP, ci^UAS.cAa, wg² has prothoracic ventral denticle belt | germline clone phenotype

(Angelats et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ci^Scer\UAS.cAa under the control of Scer\GAL4^pb.PJ in pb⁵/+ heterozygotes enhances the reduced pseudotracheal row phenotype of ci^Scer\UAS.cAa single mutants. When pb^Scer\UAS.A is overexpressed along with ci^Scer\UAS.cAa, both under the control of Scer\GAL4^pb.PJ, the pseudotracheal row phenotype is suppressed and labial palps appear almost the same as wild type.

(Joulia et al., 2005)

When ci^Scer\UAS.cAa (driven by Scer\GAL4^69B) is expressed in a wg^l-17 background the naked cell fate is induced in the thorax and the most anterior part of the abdomen. Unpigmented denticles are seen in the posterior trunk. However, when ci^Scer\UAS.cAa is driven by Scer\GAL4^αTub84B.PP in a wg^l-17 background, naked cuticle is formed in the thorax but denticles are present throughout the abdomen. If ci^Scer\UAS.cAa is driven by Scer\GAL4^wg.PM in a wg^l-17 background naked cuticle is produced in a cell autonomous manner. The addition of ci^Scer\UAS.cAa (driven by Scer\GAL4^αTub84B.PP) to arm² germ-line clones, can suppress denticle belt formation in the thorax of mutant embryos but not in the abdomen. The addition of ci^Scer\UAS.cAa (driven by Scer\GAL4^αTub84B.PP) to tsh⁸ mutant embryos, can suppress denticle belt formation in the thorax and the abdomen.

(Angelats et al., 2002)

Su(hh)II^II or Su(hh)III^III rescue the wing vein patterning of animals expressing ci^Scer\UAS.cAa under the control of Scer\GAL4^bs-1348 to almost wild type. Su(hh)IV^IV rescues to almost wild type the wing vein patterning in the anterior compartment of the wing of animals expressing ci^Scer\UAS.cAa under the control of Scer\GAL4^bs-1348.

(Haines and van Den Heuvel, 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ci^Scer\UAS.cAa

ci^UAS.cAa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Alexandre

(Alexandre et al., 1996)

Secondary FlyBase IDs

References (10)

Report Sections

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