Allele Dmel\ci^Scer\UAS.cAa

General Information
Symbol	Dmel\ci^Scer\UAS.cAa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Alexandre	FlyBase ID	FBal0051700
Feature type	allele	Associated gene	Dmel\ci

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Alexandre et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Full length ci cDNA cloned as an EagI fragment into pUAST. (Alexandre et al., 1996)
Carried in construct	P{UAS-ci.A} (Angelats et al., 2002, Dai et al., 2003, Haines and van Den Heuvel, 2000, Alexandre et al., 1999, Therond et al., 1999, Joulia et al., 2005, Alexandre et al., 1996, Huang and Kunes, 1998, Callejo et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^30A (Alexandre et al., 1996, Haines and van Den Heuvel, 2000) lethal \| embryonic stage, with Scer\GAL4^en-e16E (Alexandre et al., 1996) visible, with Scer\GAL4^bs-1348 (Haines and van Den Heuvel, 2000) visible, with Scer\GAL4^cad-em459 (Moreno and Morata, 1999)
Phenotype Manifest In
	adult hindgut, with Scer\GAL4^cad-em459 (Moreno and Morata, 1999) anal plate, with Scer\GAL4^cad-em459 (Moreno and Morata, 1999) denticle, with Scer\GAL4^69B (Angelats et al., 2002) embryonic/first instar larval cuticle, with Scer\GAL4^69B (Angelats et al., 2002) embryonic epidermis, with Scer\GAL4^69B (Angelats et al., 2002) larval abdominal segment, with Scer\GAL4^69B (Angelats et al., 2002) larval thoracic segment, with Scer\GAL4^69B (Angelats et al., 2002) pseudotracheal sensillum, with Scer\GAL4^pb.PJ (Joulia et al., 2005) wing \| proximal, with Scer\GAL4^30A (Alexandre et al., 1996) wing vein, with Scer\GAL4^bs-1348 (Haines and van Den Heuvel, 2000)
Detailed Description
	Statement Reference Overexpression of ci^Scer\UAS.cAa under the control of Scer\GAL4^pb.PJ leads to loss and disorganization of the pseudotracheal rows in the anterior compartment of the labial palps. This effect is less severe when flies are maintained at 22^oC than when maintained at 28^oC. (Joulia et al., 2005) ci^Scer\UAS.cAa (when driven by Scer\GAL4^69B) induces a reduction in the number of denticles in a variable fashion. Enlargements of thoracic segments are seen which are covered in naked cuticle. This effect is penetrant when tested at 18^oC 22^oC and 29^oC. At 29^oC variable suppression of the first abdominal belt is seen, and sometimes all denticle belts of the abdomen. (Angelats et al., 2002) Expression of ci^Scer\UAS.cAa under the control of Scer\GAL4^bs-1348 results in a mild wing vein phenotype. (Haines and van Den Heuvel, 2000) ci^Scer\UAS.cAa driven by Scer\GAL4^cad-em459 produces very large anal plates, about twice the wild-type size. Anal plate bristles are also seen on the hindgut. (Moreno and Morata, 1999) Animals expressing ci^Scer\UAS.cAa under the control of Scer\GAL4^αTub84B.PP in an hh^ts2 background that have been raised at 28^oC after hatching contain S phase lamina neuron precursors (LPCs) in the lamina. (Huang and Kunes, 1998) Scer\GAL4^30A induced expression causes lethality during late larval or pupal stages, pharate adults exhibit outgrowths from the proximal wing region. (Alexandre et al., 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^bs-1348, ci^Scer\UAS.cAa has visible phenotype, suppressible \| partially by Su(hh)IV^IV (Haines and van Den Heuvel, 2000) Scer\GAL4^bs-1348, ci^Scer\UAS.cAa has visible phenotype, suppressible by Su(hh)II^II (Haines and van Den Heuvel, 2000) Scer\GAL4^bs-1348, ci^Scer\UAS.cAa has visible phenotype, suppressible by Su(hh)III^III (Haines and van Den Heuvel, 2000)
NOT suppressed by
Statement Reference Scer\GAL4^bs-1348, ci^Scer\UAS.cAa has visible phenotype, non-suppressible by Su(hh)I^I (Haines and van Den Heuvel, 2000)
Phenotype Manifest In
Enhanced by
Statement Reference ci^Scer\UAS.cAa has embryonic epidermis phenotype, enhanceable by wg^l-17/Scer\GAL4^69B (Angelats et al., 2002) Scer\GAL4^69B, ci^Scer\UAS.cAa has denticle phenotype, enhanceable by wg^l-17 (Angelats et al., 2002) Scer\GAL4^69B, ci^Scer\UAS.cAa has embryonic/first instar larval cuticle phenotype, enhanceable by wg^l-17 (Angelats et al., 2002) Scer\GAL4^pb.PJ, ci^Scer\UAS.cAa has pseudotracheal sensillum phenotype, enhanceable by pb⁵/pb[+] (Joulia et al., 2005)
Suppressed by
Statement Reference Scer\GAL4^bs-1348, ci^Scer\UAS.cAa has wing vein phenotype, suppressible \| partially by Su(hh)IV^IV (Haines and van Den Heuvel, 2000) Scer\GAL4^bs-1348, ci^Scer\UAS.cAa has wing vein phenotype, suppressible by Su(hh)II^II (Haines and van Den Heuvel, 2000) Scer\GAL4^bs-1348, ci^Scer\UAS.cAa has wing vein phenotype, suppressible by Su(hh)III^III (Haines and van Den Heuvel, 2000) Scer\GAL4^pb.PJ, ci^Scer\UAS.cAa has pseudotracheal sensillum phenotype, suppressible by pb^Scer\UAS.A/Scer\GAL4^pb.PJ (Joulia et al., 2005)
NOT suppressed by
Statement Reference Scer\GAL4^bs-1348, ci^Scer\UAS.cAa has wing vein phenotype, non-suppressible by Su(hh)I^I (Haines and van Den Heuvel, 2000)
Other
Statement Reference Scer\GAL4^wg.PM, ci^Scer\UAS.cAa, wg^l-17 has embryonic/first instar larval cuticle phenotype (Angelats et al., 2002, Angelats et al., 2002) Scer\GAL4^αTub84B.PP, arm², ci^Scer\UAS.cAa has embryonic/first instar larval cuticle \| germline clone phenotype (Angelats et al., 2002, Angelats et al., 2002) Scer\GAL4^αTub84B.PP, arm², ci^Scer\UAS.cAa has mesothoracic ventral denticle belt \| germline clone phenotype (Angelats et al., 2002, Angelats et al., 2002) Scer\GAL4^αTub84B.PP, arm², ci^Scer\UAS.cAa has metathoracic ventral denticle belt \| germline clone phenotype (Angelats et al., 2002, Angelats et al., 2002) Scer\GAL4^αTub84B.PP, arm², ci^Scer\UAS.cAa has prothoracic ventral denticle belt \| germline clone phenotype (Angelats et al., 2002, Angelats et al., 2002) Scer\GAL4^αTub84B.PP, ci^Scer\UAS.cAa, tsh⁸ has denticle belt phenotype (Angelats et al., 2002, Angelats et al., 2002) Scer\GAL4^αTub84B.PP, ci^Scer\UAS.cAa, tsh⁸ has embryonic/first instar larval cuticle phenotype (Angelats et al., 2002, Angelats et al., 2002) Scer\GAL4^αTub84B.PP, ci^Scer\UAS.cAa, wg² has embryonic/first instar larval cuticle \| germline clone phenotype (Angelats et al., 2002, Angelats et al., 2002) Scer\GAL4^αTub84B.PP, ci^Scer\UAS.cAa, wg² has mesothoracic ventral denticle belt \| germline clone phenotype (Angelats et al., 2002, Angelats et al., 2002) Scer\GAL4^αTub84B.PP, ci^Scer\UAS.cAa, wg² has metathoracic ventral denticle belt \| germline clone phenotype (Angelats et al., 2002, Angelats et al., 2002) Scer\GAL4^αTub84B.PP, ci^Scer\UAS.cAa, wg² has prothoracic ventral denticle belt \| germline clone phenotype (Angelats et al., 2002, Angelats et al., 2002) Scer\GAL4^αTub84B.PP, ci^Scer\UAS.cAa, wg^l-17 has embryonic/first instar larval cuticle phenotype (Angelats et al., 2002, Angelats et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference Expression of ci^Scer\UAS.cAa under the control of Scer\GAL4^pb.PJ in pb⁵/+ heterozygotes enhances the reduced pseudotracheal row phenotype of ci^Scer\UAS.cAa single mutants. When pb^Scer\UAS.A is overexpressed along with ci^Scer\UAS.cAa, both under the control of Scer\GAL4^pb.PJ, the pseudotracheal row phenotype is suppressed and labial palps appear almost the same as wild type. (Joulia et al., 2005) When ci^Scer\UAS.cAa (driven by Scer\GAL4^69B) is expressed in a wg^l-17 background the naked cell fate is induced in the thorax and the most anterior part of the abdomen. Unpigmented denticles are seen in the posterior trunk. However, when ci^Scer\UAS.cAa is driven by Scer\GAL4^αTub84B.PP in a wg^l-17 background, naked cuticle is formed in the thorax but denticles are present throughout the abdomen. If ci^Scer\UAS.cAa is driven by Scer\GAL4^wg.PM in a wg^l-17 background naked cuticle is produced in a cell autonomous manner. The addition of ci^Scer\UAS.cAa (driven by Scer\GAL4^αTub84B.PP) to arm² germ-line clones, can suppress denticle belt formation in the thorax of mutant embryos but not in the abdomen. The addition of ci^Scer\UAS.cAa (driven by Scer\GAL4^αTub84B.PP) to tsh⁸ mutant embryos, can suppress denticle belt formation in the thorax and the abdomen. (Angelats et al., 2002) Su(hh)II^II or Su(hh)III^III rescue the wing vein patterning of animals expressing ci^Scer\UAS.cAa under the control of Scer\GAL4^bs-1348 to almost wild type. Su(hh)IV^IV rescues to almost wild type the wing vein patterning in the anterior compartment of the wing of animals expressing ci^Scer\UAS.cAa under the control of Scer\GAL4^bs-1348. (Haines and van Den Heuvel, 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	ci^Scer\UAS.cAa ci^UAS.cAa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Alexandre (Alexandre et al., 1996)
Secondary FlyBase IDs

References ( 10 )
Research paper	Callejo et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(31): 12591--12598 Feature Article: From the Cover: Dispatched mediates Hedgehog basolateral release to form the long-range morphogenetic gradient in the Drosophila wing disk epithelium. [FBrf0214615] Joulia et al., 2005, Dev. Biol. 278(2): 496--510 Homeotic proboscipedia function modulates hedgehog-mediated organizer activity to pattern adult Drosophila mouthparts. [FBrf0183854] Dai et al., 2003, Dev. Cell 4(6): 917--928 A hedgehog-responsive region in the Drosophila wing disc is defined by debra-mediated ubiquitination and lysosomal degradation of Ci. [FBrf0160468] Angelats et al., 2002, Dev. Biol. 241(1): 132--144 Cubitus interruptus acts to specify naked cuticle in the trunk of Drosophila embryos. [FBrf0141480] Haines and van Den Heuvel, 2000, Genetics 156(4): 1777--1785 A directed mutagenesis screen in Drosophila melanogaster reveals new mutants that influence hedgehog signaling. [FBrf0131307] Alexandre et al., 1999, Development 126(24): 5689--5698 Wingless and Hedgehog pattern Drosophila denticle belts by regulating the production of short-range signals. [FBrf0111771] Therond et al., 1999, Development 126(18): 4039--4051 Differential requirements of the Fused kinase for Hedgehog signalling in the Drosophila embryo. [FBrf0111500] Huang and Kunes, 1998, Development 125(19): 3753--3764 Signals transmitted along retinal axons in Drosophila: hedgehog signal reception and the cell circuitry of lamina cartridge assembly. [FBrf0105250] Alexandre et al., 1996, Genes Dev. 10(16): 2003--2013 Transcriptional activation of hedgehog target genes in Drosophila is mediated directly by the cubitus interruptus protein, a member of the GLI family of zinc finger DNA-binding proteins. [FBrf0089583]
Letter	Moreno and Morata, 1999, Nature 400(6747): 873--877 Caudal is the Hox gene that specifies the most posterior Drosophile segment. [FBrf0111436]

Allele Dmel\ciScer\UAS.cAa

Allele Dmel\ci^Scer\UAS.cAa