FlyBase Allele Report: Dmel\bowl[1]

General Information

Symbol

Dmel\bowl¹

Species

D. melanogaster

Name

FlyBase ID

FBal0051737

Feature type

allele

Associated gene

Dmel\bowl

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Wang and Coulter, 1996)

Mutagen

ethyl methanesulfonate

(Wang and Coulter, 1996)

Progenitor genotype

Cytology

Description

Amino acid replacement: S232term.

(Wang and Coulter, 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:3,780,420..3,780,420 [+]

Nucleotide change:

C3780420A

Reported nucleotide change:

C1039A

Amino acid change:

Reported amino acid change:

S232term

Comment:

5 residues upstream of first zinc finger

(Wang and Coulter, 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal 2 ventral denticle belt (with bowl³)

(Wang and Coulter, 1996)

abdominal 6 ventral denticle belt (with bowl³)

(Wang and Coulter, 1996)

adult cuticle & leg | somatic clone

(Hao et al., 2003)

antenna | ectopic | somatic clone

(Brás-Pereira and Casares, 2008)

cephalopharyngeal skeleton (with bowl²)

(Wang and Coulter, 1996)

embryonic/larval hindgut | embryonic stage

(Wang and Coulter, 1996)

embryonic/larval proventriculus | embryonic stage

(Wang and Coulter, 1996)

embryonic hindgut

(Iwaki et al., 2001)

embryonic large intestine

(Iwaki et al., 2001)

embryonic mandibular segment

(Wang and Coulter, 1996)

embryonic maxillary segment

(Wang and Coulter, 1996)

eye | ectopic | somatic clone

(Brás-Pereira and Casares, 2008)

eye-antennal disc | somatic clone

(Brás-Pereira and Casares, 2008)

eye disc | somatic clone

(Bras-Pereira et al., 2006)

lateral process of cephalopharyngeal skeleton (with bowl²)

(Wang and Coulter, 1996)

leg joint | somatic clone

(Hao et al., 2003)

peripodial epithelium & dorsal mesothoracic disc | somatic clone | first instar larval stage

(Nusinow et al., 2008)

prescutum | somatic clone

(Del Signore et al., 2012)

tarsal segment | somatic clone

(Hao et al., 2003)

Detailed Description

Statement

Reference

Homozygous clones generate cuticle patterning defects in the prescutum.

Homozygous clones induced in the third larval instar adopt a round and compact morphology and tend to segregate from the surrounding bowl[+] cells.

(Del Signore et al., 2012)

Somatic mutant clones induced in the antennal disc during L1 result in ectopic antennae formation, containing both proximal and distal antennal segments.

Mutant somatic clones in the ventral-posterior rim of the antennal disc lobe in L1 result in ventral eyelet formation and differentiation of mutant cells into photoreceptors in the adult eye.

(Brás-Pereira and Casares, 2008)

Most homozygous clones generated at the early first larval instar in the disc proper of the wing disc survive.

Only 50% of homozygous clones generated at the early first larval instar in the peripodial epithelium of the wing disc survive, with most surviving near the disc stalk.

Homozygous clones generated at the second larval instar in the peripodial epithelium or disc proper of the wing disc both survive.

(Nusinow et al., 2008)

Homozygous clones in the eye disc (induced during the first larval instar) that span the margin cause either a delay in, or the inhibition of, retinal initiation.

(Bras-Pereira et al., 2006)

Mutant clones in the distal parts of the leg are associated with tarsal segment fusions, as the joints fail to form within the mutant tissue. This joint loss is cell autonomous. In some cases where the mutant clone only forms a small part of the leg, no defects in segmentation are seen. In the proximal part of the leg, mutant clones are not associated with leg segment fusion, but rather with dark melanotic protrusions from the leg cuticle, which always occur near the site of an endogenous joint. Legs comprised almost entirely of mutant clone tissue (large clones induced using the Minute technique) show a failure of joint formation in segments extending from the tibia to tarsal segment 5, but proximal joints form normally.

(Hao et al., 2003)

Mutant embryos at stage 16 have hindguts about half the size of wild-type. These hindguts have larger lumens and two or three more cells in their circumference than those of wild-type. Hindguts are club shaped and smaller in both length and diameter. Hindgut epithelial cells are taller and narrower than seen in wild-type. The "boundary cells" that normally run the length of the large intestine are duplicated. Cell proliferation and apoptosis appear normal in mutant hindguts. Hindguts contain slightly fewer cells than wild-type. The visceral mesoderm is also normal in these mutants.

(Iwaki et al., 2001)

Homozygous embryos exhibit the 'tail-up' phenotype and defects in the mandible and maxilla. Hindgut is drastically reduced, proventriculus is absent. Second abdominal denticle belt is missing and the sixth abdominal denticle belt is defective in bowl¹/bowl³ transheterozygotes. Lateralgraten is shortened and the ventral arm reduced in bowl¹/bowl² transheterozygotes.

(Wang and Coulter, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

bowl¹ has visible | somatic clone phenotype, non-suppressible by Antp^73b

(Brás-Pereira and Casares, 2008)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

bowl¹ has embryonic foregut phenotype, non-enhanceable by lin^unspecified

(Johansen et al., 2003)

bowl¹ has keyhole structure phenotype, non-enhanceable by lin^unspecified

(Johansen et al., 2003)

bowl¹, lin^unspecified has embryonic foregut phenotype, non-enhanceable by Df(2L)drm-P2

(Johansen et al., 2003)

bowl¹, lin^unspecified has keyhole structure phenotype, non-enhanceable by Df(2L)drm-P2

(Johansen et al., 2003)

bowl¹, lin^unspecified has embryonic foregut phenotype, non-enhanceable by drm³

(Johansen et al., 2003)

bowl¹, lin^unspecified has keyhole structure phenotype, non-enhanceable by drm³

(Johansen et al., 2003)

NOT suppressed by

Statement

Reference

bowl¹ has eye-antennal disc | somatic clone phenotype, non-suppressible by Antp^73b

(Brás-Pereira and Casares, 2008)

bowl¹, lin^unspecified has embryonic foregut phenotype, non-suppressible by Df(2L)drm-P2

(Johansen et al., 2003)

bowl¹, lin^unspecified has embryonic foregut phenotype, non-suppressible by drm³

(Johansen et al., 2003)

bowl¹, lin^unspecified has keyhole structure phenotype, non-suppressible by Df(2L)drm-P2

(Johansen et al., 2003)

bowl¹, lin^unspecified has keyhole structure phenotype, non-suppressible by drm³

(Johansen et al., 2003)

bowl¹ has keyhole structure phenotype, non-suppressible by lin^unspecified

(Johansen et al., 2003)

bowl¹ has embryonic foregut phenotype, non-suppressible by lin^unspecified

(Johansen et al., 2003)

Suppressor of

Statement

Reference

bowl¹ is a suppressor of eye disc | somatic clone phenotype of Scer\GAL4^Act5C.PP, drm^UAS.cGa

(Bras-Pereira et al., 2006)

Other

Statement

Reference

bowl¹, lin^unspecified has keyhole structure phenotype

(Johansen et al., 2003)

bowl¹, lin^unspecified has embryonic foregut phenotype

(Johansen et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Somatic mutant bowl¹ clones induced in the ventral region of the antennal imaginal disc during stage L1 in Antp^73b mutant larvae result in the formation of ectopic, supernumerary appendages.

(Brás-Pereira and Casares, 2008)

Expression of drm^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PP in clones in the eye disc in a bowl¹ background does not result in ectopic retinogenesis.

(Bras-Pereira et al., 2006)

drm³ bowl¹ double mutant clones in the leg (induced using the Minute technique) have a leg segment fusion phenotype that can be attributed to the loss of bowl alone.

(Hao et al., 2003)

The gut morphology of Df(2L)drm-P2, bowl¹homozygous embryos or drm³, bowl¹ double homozygous embryos, is indistinguishable from that of either single mutant. lin^unspecified; bowl¹ double homozygous embryos have a foregut phenotype identical to those of bowl¹ homozygotes (narrow and lacking a keyhole structure), rather than lin^unspecified homozygotes (bloated and with an expanded keyhole structure). This phenotype is unaffected in embryos also homozygous for drm³, or Df(2L)drm-P2.

(Johansen et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

7094

bowl¹ pr¹ cn¹/CyO

9900

w^*; bowl¹ P{neoFRT}40A/CyO, P{ActGFP}JMR1

9903

y¹ w^*; drm³ bowl¹ P{neoFRT}40A/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

bowl¹

(Wen et al., 2024, Bourbon et al., 2022, Kahsai and Cook, 2018, Mojica-Vázquez et al., 2017, Del Signore et al., 2012, Greenberg and Hatini, 2009, Brás-Pereira and Casares, 2008, Nusinow et al., 2008, Tena et al., 2007, Bras-Pereira et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (17)

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