Allele Dmel\NetB^{Scer\UAS.T:Hsap\MYC}

General Information
Symbol	Dmel\NetB^{Scer\UAS.T:Hsap\MYC}	Species	D. melanogaster
Name		FlyBase ID	FBal0052146
Feature type	allele	Associated gene	Dmel\NetB

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Mitchell et al., 1996) in vitro construct - regulatory fusion (Mitchell et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Full length NetB cDNA and C-terminal c-myc epitope are cloned into pUASt. (Mitchell et al., 1996)
Carried in construct	P{UAS-NetrinB} (Kolesnikov and Beckendorf, 2005, Keleman and Dickson, 2001, Mitchell et al., 1996, Sano et al., 2005, von Hilchen et al., 2010, Timofeev et al., 2012)
Tagged with	T:Hsap\MYC (Keleman and Dickson, 2001, Kolesnikov and Beckendorf, 2005, Mitchell et al., 1996)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with Scer\GAL4^how-24B (Keleman and Dickson, 2001)
Phenotype Manifest In
	abdominal segmental nerve, with Scer\GAL4^how-24B (Keleman and Dickson, 2001) axon, with Scer\GAL4^elav.PLu, Scer\GAL4^sca-537.4 (Mitchell et al., 1996) commissure, with Scer\GAL4^elav.PLu, Scer\GAL4^sca-537.4 (Mitchell et al., 1996) intersegmental nerve, with Scer\GAL4^how-24B (Mitchell et al., 1996) optic lobe, with Df(1)NetAB^Δ, Scer\GAL4^MH56 (Timofeev et al., 2012) photoreceptor cell R8 stalk, with Df(1)NetAB^Δ, Scer\GAL4^MH56 (Timofeev et al., 2012) presumptive embryonic salivary gland, with Scer\GAL4^sca.PU (Kolesnikov and Beckendorf, 2005) presumptive embryonic salivary gland, with Scer\GAL4^twi.PB (Kolesnikov and Beckendorf, 2005)
Detailed Description
	Statement Reference Overexpression of NetB[Scer\UAS.T:Hsap\MYC], under the control of Scer\GAL4[MH56], which has strong activity in the lamina neurons L3 throughout pupal development, does not interfere with R8 axon targeting. Expression of NetB[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[MH56] in a Df(1)NetAB[Δ] background significantly rescues the R8 axon-targeting defects. Only 8% of Rh6-expressing neurons stall at the medulla neuropil border or terminate in the distal M1/M2 layers, compared to 61% in mutant siblings lacking NetB[Scer\UAS.T:Hsap\MYC]. (Timofeev et al., 2012) Ectopic expression of NetB^{Scer\UAS.T:Hsap\MYC} throughout the somatic and visceral mesoderm, driven by Scer\GAL4^twi.PB, causes salivary gland guidance defects. In 16% of these embryos, the distal tip of the salivary gland splits into two branches; one branch continues to migrate along the visceral mesoderm, while the other migrates towards lateral somatic muscles. Expression of NetB^{Scer\UAS.T:Hsap\MYC} throughout the CNS, driven by Scer\GAL4^sca.PU, causes the distal tip of the salivary gland to become curved medially toward the CNS, instead of lying parallel to the CNS. However, expression of NetB^{Scer\UAS.T:Hsap\MYC} in the CNS with Scer\GAL4^elav.PLu, does not cause misorientation of the salivary gland. (Kolesnikov and Beckendorf, 2005) When expression is driven by Scer\GAL4^how-24B SNa axons often stall at the edge of the CNS or fasciculate with the ISN. (Keleman and Dickson, 2001) Scer\GAL4^sca-537.4 and Scer\GAL4^elav.PLu induced expression in embryos causes commissures that are thinner or nearly absent and bundles of axons that project inappropriately laterally toward the edge of the CNS leaving gaps in the longitudinal tracts. Scer\GAL4^how-24B induced expression causes excessive branching of the intersegmental nerve and these branches to stall short of their targets. (Mitchell et al., 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference NetB^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^how-24B/Scer\GAL4^how-24B has neuroanatomy defective phenotype, suppressible by unc-5^dsRNA.cKa, Scer\GAL4^how-24B/Scer\GAL4^how-24B (Keleman and Dickson, 2001)
NOT suppressed by
Statement Reference NetB^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^how-24B/Scer\GAL4^how-24B has neuroanatomy defective phenotype, non-suppressible by Ptp69D^dsRNA.cKa, Scer\GAL4^how-24B/Scer\GAL4^how-24B (Keleman and Dickson, 2001)
Phenotype Manifest In
Suppressed by
Statement Reference NetB^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^how-24B/Scer\GAL4^how-24B has abdominal segmental nerve phenotype, suppressible by unc-5^dsRNA.cKa, Scer\GAL4^how-24B/Scer\GAL4^how-24B (Keleman and Dickson, 2001)
NOT suppressed by
Statement Reference NetB^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^how-24B/Scer\GAL4^how-24B has abdominal segmental nerve phenotype, non-suppressible by Ptp69D^dsRNA.cKa, Scer\GAL4^how-24B/Scer\GAL4^how-24B (Keleman and Dickson, 2001)
Additional Comments
Genetic Interactions
Statement Reference The SNa pathfinding defect can be suppressed by injection of unc-5^dsRNA.cKa RNA. (Keleman and Dickson, 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments	Expression of NetB[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[sim.PS] only weakly rescues the defects in interface glial cell migration seen in Df(1)NetAB[Δ] embryos. A clear rescue of the stalling phenotype of the peripheral embryonic glia is seen in these animals. Expression of NetB[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[sca-537.4] completely rescues the defects in interface glial cell migration seen in Df(1)NetAB[Δ] embryos in 90% of embryos. (von Hilchen et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	NetB^{Scer\UAS.T:Hsap\MYC} NetB^UAS.T:Myc
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Timofeev et al., 2012, Neuron 75(1): 80--93 Localized netrins act as positional cues to control layer-specific targeting of photoreceptor axons in Drosophila. [FBrf0218874] von Hilchen et al., 2010, Development 137(8): 1251--1262 Netrins guide migration of distinct glial cells in the Drosophila embryo. [FBrf0210391] Kolesnikov and Beckendorf, 2005, Dev. Biol. 284(1): 102--111 NETRIN and SLIT guide salivary gland migration. [FBrf0187388] Sano et al., 2005, J. Cell Biol. 171(4): 675--683 Control of lateral migration and germ cell elimination by the Drosophila melanogaster lipid phosphate phosphatases Wunen and Wunen 2. [FBrf0190943] Keleman and Dickson, 2001, Neuron 32(4): 605--617 Short- and long-range repulsion by the Drosophila Unc5 Netrin receptor. [FBrf0141688] Mitchell et al., 1996, Neuron 17(2): 203--215 Genetic analysis of Netrin genes in Drosophila: Netrins guide CNS commissural axons and peripheral motor axons. [FBrf0089749]

Allele Dmel\NetBScer\UAS.T:Hsap\MYC

Allele Dmel\NetB^{Scer\UAS.T:Hsap\MYC}