FlyBase Allele Report: Dmel\N[UAS.cBb]

General Information

Symbol

Dmel\N^UAS.cBb

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct b of Baylies

FlyBase ID

FBal0052152

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{UAS-N.Bb}

Key Links

Transgenic product class

wild_type

(Couso et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Couso et al., 1995)

Mutagen

in vitro construct

(Klein and Martinez Arias, 1998, Couso et al., 1995)

Progenitor genotype

Carried in construct

P{UAS-N.Bb}

Cytology

Description

UAS regulatory sequences drive expression of N.

(Couso et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^bs-1348

(de Celis and Bray, 2000)

visible, with Scer\GAL4^Ubx.PdC

(de Celis and Bray, 1997)

Phenotype Manifest In

wing, with Scer\GAL4^bs-1348

(de Celis and Bray, 2000)

wing, with Scer\GAL4^Bx-MS1096

(Klein et al., 1997)

wing, with Scer\GAL4^Ubx.PdC

(de Celis and Bray, 1997)

wing & macrochaeta, with Scer\GAL4^sd-SG29.1

(Klein et al., 1997)

wing vein, with Scer\GAL4^bs-1348

(de Celis and Bray, 2000)

wing vein, with Scer\GAL4^ptc-559.1

(Klein et al., 1997)

wing vein, with Scer\GAL4^sd-SG29.1

(Klein et al., 1997)

Detailed Description

Statement

Reference

Flies expressing N^Scer\UAS.cBb under the control of Scer\GAL4^bs-1348 show a wing phenotype similar to Dp(1;2)51b flies.

(de Celis and Bray, 2000)

Scer\GAL4^sd-SG29.1-mediated expression does not change the general shape of the wing but leads to a mixture of neurogenic and antineurogenic effects: thickened veins and loss of bristles at the wing margin. Scer\GAL4^Bx-MS1096-mediated expression leads to deletions of the anterior region of the wing and thickened veins. Scer\GAL4^ptc-559.1-mediated expression produces small overgrowths at the distal part of the wing.

(Klein et al., 1997)

When expression is driven by Scer\GAL4^Ubx.PdC in clones in the wing, outpocketings of the wing occur which include both N^{int.G.Scer\UAS}-expressing and neighboring wild type cells. Ectopic wing margin bristles appear in both mutant and wild type cells. The cells are smaller and have altered polarity. The effects are similar to but milder than those seen for N^{int.G.Scer\UAS}.

(de Celis and Bray, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of Ser^Scer\UAS.cSa and N^Scer\UAS.cBb under the control of Scer\GAL4^sd-SG29.1 generates very large wings with a clearly defined margin with an antineurogenic phenotype. Coexpression under the control of Scer\GAL4^Bx-MS1096 results in normally shaped wings in which the dorsal surface has overgrown the ventral one. Coexpression under the control of Scer\GAL4^ptc-559.1 causes ectopic ventral double margin is transformed into a single thick one and the ventral margin and associated overgrowth are extended into the dorsal surface of the wing.

(Klein et al., 1997)

Scer\GAL4^h-1J3 induced coexpression of wg^{l-12.Scer\UAS} and Ser^Scer\UAS.cSa fails to cause ectopic wings, expression of P{UAS-N.Bb} with both wg^{l-12.Scer\UAS} and Ser^Scer\UAS.cSa causes ectopic wing development.

(Couso et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

N^Scer\UAS.cBb

N^UAS.cBb

N^fl

(de Celis and Bray, 1997)

Name Synonyms

Saccharomyces cerevisiae UAS construct b of Baylies

(Couso et al., 1995)

Secondary FlyBase IDs

References (9)

Report Sections

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