Allele Dmel\Mad^Scer\UAS.cNa

General Information
Symbol	Dmel\Mad^Scer\UAS.cNa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Newfeld	FlyBase ID	FBal0052172
Feature type	allele	Associated gene	Dmel\Mad

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Newfeld et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of the complete Mad cDNA is governed by Scer\UAS regulatory sequences. (Newfeld et al., 1996)
Carried in construct	P{UAS-Mad.N} (Moser and Campbell, 2005, Takaesu et al., 2005, McCabe et al., 2004, Tsuneizumi et al., 1997, Newfeld et al., 1997, Marquez et al., 2001, Newfeld et al., 1996, Takaesu et al., 2006, Letizia et al., 2007, Gesualdi and Haerry, 2007, Quijano et al., 2011, Zeng et al., 2007, Barrio et al., 2007, Koh et al., 2004, Takaesu et al., 2012, Fischer et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	size defective, with Scer\GAL4^A9 (Fischer et al., 2012) visible, with Scer\GAL4^ptc-559.1 (Marquez et al., 2001) visible, with Scer\GAL4^sca-537.4 (Quijano et al., 2011) visible, with Scer\GAL4^vg.int2.1 (Zeng et al., 2007) visible, with Scer\GAL4^vg.PM (Tsuneizumi et al., 1997)
Phenotype Manifest In
	leg \| ectopic, with Scer\GAL4^ptc-559.1 (Marquez et al., 2001) wing (Gesualdi and Haerry, 2007) wing, with Scer\GAL4^nub-AC-62 (Barrio et al., 2007) wing, with Scer\GAL4^ptc-559.1 (Marquez et al., 2001) wing, with Scer\GAL4^T80 (Takaesu et al., 2012) wing, with Scer\GAL4^vg.int2.1 (Zeng et al., 2007) wing, with Scer\GAL4^vg.PM (Tsuneizumi et al., 1997) wing disc (Gesualdi and Haerry, 2007) wing vein (Gesualdi and Haerry, 2007) wing vein, with Scer\GAL4^nub-AC-62 (Barrio et al., 2007) wing vein \| ectopic, with Scer\GAL4^A9 (Fischer et al., 2012) wing vein \| ectopic, with Scer\GAL4^ptc-559.1 (Marquez et al., 2001, Takaesu et al., 2005) wing vein \| ectopic, with Scer\GAL4^sca-537.4 (Quijano et al., 2011) wing vein \| ectopic, with Scer\GAL4^vg.int2.1 (Zeng et al., 2007)
Detailed Description
	Statement Reference Overexpression of Mad[Scer\UAS.cNa] with Scer\GAL4[A9] leads to a dramatic reduction in wing size, ectopic vein tissue and blistering. (Fischer et al., 2012) Expression of Mad[Scer\UAS.cNa] in the wing discs under the control of Scer\GAL4[T80] leads to wing vein truncation. (Takaesu et al., 2012) Expression of Mad[Scer\UAS.cNa] under the control of Scer\GAL4[sca-537.4] results in wild-type wings 34% of the time, while the remaining wings have a variety of mild ectopic vein phenotypes. (Quijano et al., 2011) Expression of Mad[Scer\UAS.cNa] using Scer\GAL4[salm-459.2] has no severe effects on wing growth or patterning. Expression of Mad[Scer\UAS.cNa] using Scer\GAL4[nub-AC-62] causes alterations in the vein pattern and wing size. (Barrio et al., 2007) Overexpression of Mad^Scer\UAS.cNa under the control of Scer\GAL4^A9 results in wings of almost normal size that exhibit some ectopic vein tissue. (Gesualdi and Haerry, 2007) Expression of Mad[Scer\UAS.cNa] under the control of Scer\GAL4[vg.int2.1] results in a broader wing and ectopic veins along L2 and L5 and emanating from the posterior crossvein. (Zeng et al., 2007) Expression of Mad^Scer\UAS.cNa under the control of Scer\GAL4^ptc-559.1 produces excess vein tissue between L3/L4/L5 wing veins. (Takaesu et al., 2005) When expression is driven by Scer\GAL4^ptc-559.1, ectopic vein tissue appears between wing veins L3 and L5. The distance between L3 and L5 appears reduced. Wings are 22% smaller than wild type. An ectopic leg develops on the ventral side of the normal limb. The ectopic leg is well formed with several segments and tarsal claws. (Marquez et al., 2001) When expression is driven by Scer\GAL4^vg.PM, outgrowth of wing tissue results. (Tsuneizumi et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Mad^Scer\UAS.cNa, Scer\GAL4^vg.int2.1 has visible phenotype, enhanceable by rl[+]/rl^Sem (Zeng et al., 2007) Mad^Scer\UAS.cNa, Scer\GAL4^vg.int2.1 has visible phenotype, enhanceable by rl^{Sem.S.Scer\UAS}, Scer\GAL4^vg.int2.1 (Zeng et al., 2007)
Suppressed by
Statement Reference Mad^Scer\UAS.cNa, Scer\GAL4^A9 has size defective phenotype, suppressible by fuss^Scer\UAS.C, Scer\GAL4^A9 (Fischer et al., 2012) Mad^Scer\UAS.cNa, Scer\GAL4^vg.int2.1 has visible phenotype, suppressible by nmo^Scer\UAS.cUa, Scer\GAL4^vg.int2.1 (Zeng et al., 2007) Mad^Scer\UAS.cNa, Scer\GAL4^vg.PM has visible phenotype, suppressible by Dad^Scer\UAS.cTa, Scer\GAL4^vg.PM (Tsuneizumi et al., 1997)
Suppressor of
Statement Reference Mad^Scer\UAS.cNa, Scer\GAL4^A9 is a suppressor \| partially of visible phenotype of Scer\GAL4^A9, Snoo^Scer\UAS.cTa (Takaesu et al., 2006)
NOT Suppressor of
Statement Reference Mad^Scer\UAS.cNa, Scer\GAL4^elav-C155 is a non-suppressor of locomotor behavior defective \| heat sensitive phenotype of Hsap\TOR1A^ΔE.Scer\UAS, Scer\GAL4^elav-C155 (Koh et al., 2004)
Other
Statement Reference Axn^{ΔRGS.Scer\UAS}, Mad^Scer\UAS.cNa, Scer\GAL4^sca-537.4 has visible phenotype (Quijano et al., 2011) Mad^Scer\UAS.cNa, Scer\GAL4^sca-537.4, dsh^Scer\UAS.cAa has visible phenotype (Quijano et al., 2011) Mad^Scer\UAS.cNa, Scer\GAL4^sca-537.4, sgg^{A81T.Scer\UAS} has visible phenotype (Quijano et al., 2011) Mad^Scer\UAS.cNa, Scer\GAL4^sca-537.4, sgg^S9A.Scer\UAS has visible phenotype (Quijano et al., 2011)
Phenotype Manifest In
Enhanced by
Statement Reference Mad^Scer\UAS.cNa, Scer\GAL4^vg.int2.1 has wing phenotype, enhanceable by rl[+]/rl^Sem (Zeng et al., 2007) Mad^Scer\UAS.cNa, Scer\GAL4^vg.int2.1 has wing phenotype, enhanceable by rl^{Sem.S.Scer\UAS}, Scer\GAL4^vg.int2.1 (Zeng et al., 2007) Mad^Scer\UAS.cNa, Scer\GAL4^vg.int2.1 has wing vein \| ectopic phenotype, enhanceable by rl[+]/rl^Sem (Zeng et al., 2007) Mad^Scer\UAS.cNa, Scer\GAL4^vg.int2.1 has wing vein \| ectopic phenotype, enhanceable by rl^{Sem.S.Scer\UAS}, Scer\GAL4^vg.int2.1 (Zeng et al., 2007) Mad^Scer\UAS.cNa has wing disc phenotype, enhanceable by Scer\GAL4^A9/babo^{Q302D.Scer\UAS} (Gesualdi and Haerry, 2007) Mad^Scer\UAS.cNa has wing phenotype, enhanceable by Scer\GAL4^A9/babo^{Q302D.Scer\UAS} (Gesualdi and Haerry, 2007) Mad^Scer\UAS.cNa has wing vein phenotype, enhanceable by Scer\GAL4^A9/babo^{Q302D.Scer\UAS} (Gesualdi and Haerry, 2007)
Suppressed by
Statement Reference Mad^Scer\UAS.cNa, Scer\GAL4^A9 has wing vein \| ectopic phenotype, suppressible \| partially by fuss^Scer\UAS.C, Scer\GAL4^A9 (Fischer et al., 2012) Mad^Scer\UAS.cNa, Scer\GAL4^nub-AC-62 has wing phenotype, suppressible \| partially by Snoo^M67/Snoo^2c3, Scer\GAL4^nub-AC-62 (Barrio et al., 2007) Mad^Scer\UAS.cNa, Scer\GAL4^nub-AC-62 has wing phenotype, suppressible by Snoo^M67, Scer\GAL4^nub-AC-62 (Barrio et al., 2007) Mad^Scer\UAS.cNa, Scer\GAL4^nub-AC-62 has wing vein phenotype, suppressible \| partially by Snoo^M67/Snoo^2c3, Scer\GAL4^nub-AC-62 (Barrio et al., 2007) Mad^Scer\UAS.cNa, Scer\GAL4^nub-AC-62 has wing vein phenotype, suppressible by Snoo^M67, Scer\GAL4^nub-AC-62 (Barrio et al., 2007) Mad^Scer\UAS.cNa, Scer\GAL4^vg.int2.1 has wing phenotype, suppressible by nmo^Scer\UAS.cUa, Scer\GAL4^vg.int2.1 (Zeng et al., 2007) Mad^Scer\UAS.cNa, Scer\GAL4^vg.int2.1 has wing vein \| ectopic phenotype, suppressible by nmo^Scer\UAS.cUa, Scer\GAL4^vg.int2.1 (Zeng et al., 2007) Mad^Scer\UAS.cNa, Scer\GAL4^vg.PM has wing phenotype, suppressible by Dad^Scer\UAS.cTa, Scer\GAL4^vg.PM (Tsuneizumi et al., 1997) Mad^Scer\UAS.cNa has wing phenotype, suppressible by Scer\GAL4^A9/Actβ^CM.Scer\UAS (Gesualdi and Haerry, 2007) Mad^Scer\UAS.cNa has wing phenotype, suppressible by Scer\GAL4^A9/myo^Scer\UAS.cGa (Gesualdi and Haerry, 2007) Mad^Scer\UAS.cNa has wing vein phenotype, suppressible by Scer\GAL4^A9/Actβ^CM.Scer\UAS (Gesualdi and Haerry, 2007) Mad^Scer\UAS.cNa has wing vein phenotype, suppressible by Scer\GAL4^A9/myo^Scer\UAS.cGa (Gesualdi and Haerry, 2007)
Enhancer of
Statement Reference Scer\GAL4^A9/Mad^Scer\UAS.cNa is an enhancer of wing disc phenotype of babo^{Q302D.Scer\UAS} (Gesualdi and Haerry, 2007) Scer\GAL4^A9/Mad^Scer\UAS.cNa is an enhancer of wing vein phenotype of babo^{Q302D.Scer\UAS} (Gesualdi and Haerry, 2007)
Suppressor of
Statement Reference Mad^Scer\UAS.cNa, Scer\GAL4^A9 is a suppressor \| partially of wing phenotype of Scer\GAL4^A9, Snoo^Scer\UAS.cTa (Takaesu et al., 2006) Mad^Scer\UAS.cNa, Scer\GAL4^A9 is a suppressor \| partially of wing vein phenotype of Scer\GAL4^A9, Snoo^Scer\UAS.cTa (Takaesu et al., 2006) Mad^Scer\UAS.cNa, Scer\GAL4^nub-AC-62 is a suppressor of wing phenotype of Scer\GAL4^nub-AC-62, Snoo^M67 (Barrio et al., 2007) Mad^Scer\UAS.cNa, Scer\GAL4^nub-AC-62 is a suppressor of wing vein phenotype of Scer\GAL4^nub-AC-62, Snoo^M67 (Barrio et al., 2007) Mad^Scer\UAS.cNa, Scer\GAL4^salm-459.2 is a suppressor of wing phenotype of Scer\GAL4^salm-459.2, Snoo^M67 (Barrio et al., 2007) Mad^Scer\UAS.cNa, Scer\GAL4^salm-459.2 is a suppressor of wing vein phenotype of Scer\GAL4^salm-459.2, Snoo^M67 (Barrio et al., 2007) Scer\GAL4^A9/Mad^Scer\UAS.cNa is a suppressor of wing phenotype of babo^{Q302D.Scer\UAS} (Gesualdi and Haerry, 2007)
Other
Statement Reference Axn^{ΔRGS.Scer\UAS}, Mad^Scer\UAS.cNa, Scer\GAL4^sca-537.4 has wing margin bristle phenotype (Quijano et al., 2011) Mad^Scer\UAS.cNa, Scer\GAL4^A9, daw^Scer\UAS.cGa has wing phenotype (Gesualdi and Haerry, 2007, Gesualdi and Haerry, 2007) Mad^Scer\UAS.cNa, Scer\GAL4^sca-537.4, dsh^Scer\UAS.cAa has wing margin bristle \| ectopic phenotype (Quijano et al., 2011) Mad^Scer\UAS.cNa, Scer\GAL4^sca-537.4, dsh^Scer\UAS.cAa has wing sensillum phenotype (Quijano et al., 2011) Mad^Scer\UAS.cNa, Scer\GAL4^sca-537.4, dsh^Scer\UAS.cAa has wing vein L3 \| distal phenotype (Quijano et al., 2011) Mad^Scer\UAS.cNa, Scer\GAL4^sca-537.4, sgg^{A81T.Scer\UAS} has wing margin bristle \| ectopic phenotype (Quijano et al., 2011) Mad^Scer\UAS.cNa, Scer\GAL4^sca-537.4, sgg^{A81T.Scer\UAS} has wing sensillum \| ectopic phenotype (Quijano et al., 2011) Mad^Scer\UAS.cNa, Scer\GAL4^sca-537.4, sgg^{A81T.Scer\UAS} has wing vein L3 phenotype (Quijano et al., 2011) Mad^Scer\UAS.cNa, Scer\GAL4^sca-537.4, sgg^S9A.Scer\UAS has wing margin bristle phenotype (Quijano et al., 2011) Mad^Scer\UAS.cNa, Scer\GAL4^sca-537.4, sgg^S9A.Scer\UAS has wing sensillum phenotype (Quijano et al., 2011) Mad^Scer\UAS.cNa, Scer\GAL4^sca-537.4, sgg^S9A.Scer\UAS has wing vein phenotype (Quijano et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Coexpression of fuss[Scer\UAS.C] in a Mad[Scer\UAS.cNa], Scer\GAL4[A9] background restores not only the size of the wing, but leads to a notable amelioration of the vein-intervein patterning. (Fischer et al., 2012) Co-expression of sgg[S9A.Scer\UAS] and Mad[Scer\UAS.cNa] under the control of Scer\GAL4[sca-537.4] results in wings with no L1 or L3 sensilla, missing margin bristles of all types and occasional vein truncations. Co-expression of dsh[Scer\UAS.cAa] and Mad[Scer\UAS.cNa] under the control of Scer\GAL4[sca-537.4] results in wings with no L1 or L3 sensilla, ectopic mechanosensory bristles on the dorsal margin and expansion of the L3 vein distally. Co-expression of sgg[A81T.Scer\UAS] and Mad[Scer\UAS.cNa] under the control of Scer\GAL4[sca-537.4] results in wings with ectopic sensilla on a slightly thickened L3 vein and ectopic mechanosensory bristles on the dorsal margin. Flies co-expressing Axn[ΔRGS.Scer\UAS] and Mad[Scer\UAS.cNa] under the control of Scer\GAL4[sca-537.4] have normal wing sensilla with occasional small gaps in the row or stout mechanosensory bristles on the margin. (Quijano et al., 2011) Co-expression of Mad[Scer\UAS.cNa] suppresses the wing phenotypes caused by Scer\GAL4[salm-459.2]-mediated expression of Snoo[M67], giving rise to wings of almost normal size and pattern. Co-expression of Mad[Scer\UAS.cNa] and Snoo[M67] using Scer\GAL4[nub-AC-62] results in wings with a normal pattern of veins. Scer\GAL4[MD-638]-mediated expression of the Snoo[2c3] mutant via the P{GSV1} element associated with Snoo[M67] partially suppresses the wing phenotype caused by Scer\GAL4[MD-638]-mediated expression of Mad[Scer\UAS.cNa]. (Barrio et al., 2007) Co-expression of babo^{Q302D.Scer\UAS} and Mad^Scer\UAS.cNa, both under the control of Scer\GAL4^A9, increases the transformation of intervein into vein tissue. Although the wing discs are larger, the adult wings are smaller due to cell death and transformation of intervein into more tightly associated vein cells. Co-expression of myoglianin^Scer\UAS.cGa with Mad^Scer\UAS.cNa, both under the control of Scer\GAL4^A9, represses the pattern defects caused by excess Mad^Scer\UAS.cNa. Co-expression of Actβ^CM.Scer\UAS and Mad^Scer\UAS.cNa, both under the control of Scer\GAL4^A9 dramatically enhances the ectopic wing vein formation seen in Mad^Scer\UAS.cNa mutants alone. Co-expression of daw^Scer\UAS.cGa and Mad^Scer\UAS.cNa, under the control of Scer\GAL4^A9, decreases the wing size to 88% without affecting ectopic vein formation. Male wing discs are smaller in size in these double mutants. (Gesualdi and Haerry, 2007) Co-expression of nmo[Scer\UAS.cUa] suppresses the mutant wing phenotype caused by expression of Mad[Scer\UAS.cNa] under the control of Scer\GAL4[vg.int2.1]. rl[Sem]/+ enhances the mutant wing phenotype caused by expression of Mad[Scer\UAS.cNa] under the control of Scer\GAL4[vg.int2.1]. Co-expression of rl[Sem.S.Scer\UAS] enhances the mutant wing phenotype caused by expression of Mad[Scer\UAS.cNa] under the control of Scer\GAL4[vg.int2.1]. (Zeng et al., 2007) Co-expression of Mad^Scer\UAS.cNa partially rescues the phenotype caused by expression of Snoo^Scer\UAS.cTa under the control of Scer\GAL4^A9; wing size is rescued to wild type and the penetrance of the multiple wing vein defects is reduced to 19%. (Takaesu et al., 2006) Dad^Scer\UAS.cTa suppresses the wing outgrowth phenotype of Mad^Scer\UAS.cNa driven by Scer\GAL4^vg.PM, resulting in an almost wild type wing. (Tsuneizumi et al., 1997)
Xenogenetic Interactions
Statement Reference Expression of Mad[Scer\UAS.cNa] in flies expressing Hsap\TOR1A[ΔE.Scer\UAS] under the control of Scer\GAL4[elav-C155] does not suppress the locomotor defects observed in flies expressing Hsap\TOR1A[ΔE.Scer\UAS] alone. (Koh et al., 2004)
Complementation & Rescue Data
Rescues	Mad^Scer\UAS.cNa/Scer\GAL4^elav.PLu rescues Df(2L)C28/Mad¹ (McCabe et al., 2004)
Comments	Expression of Mad^Scer\UAS.cNa under the control of Scer\GAL4^elav.PLu in the neurons rescues neuromuscular junction size in Mad¹/Df(2L)C28 mutants. (McCabe et al., 2004)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Mad^Scer\UAS.cNa Mad^ScerUAS.cNa (Moser and Campbell, 2005) Mad^UAS.cNa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Newfeld (Newfeld et al., 1996)
Secondary FlyBase IDs

References ( 16 )
Research paper	Fischer et al., 2012, PLoS ONE 7(8): e42349 fussel (fuss) - A Negative Regulator of BMP Signaling in Drosophila melanogaster. [FBrf0219160] Takaesu et al., 2012, Development 139(18): 3392--3401 Drosophila CORL is required for Smad2-mediated activation of Ecdysone Receptor expression in the mushroom body. [FBrf0219224] Quijano et al., 2011, Genetics 189(3): 809--824 Wg Signaling via Zw3 and Mad Restricts Self-Renewal of Sensory Organ Precursor Cells in Drosophila. [FBrf0216675] Barrio et al., 2007, Dev. Biol. 306(1): 66--81 Characterization of dSnoN and its relationship to Decapentaplegic signaling in Drosophila. [FBrf0200635] Gesualdi and Haerry, 2007, Fly 1(4): 212--221 Distinct signaling of Drosophila Activin/TGF-beta family members. [FBrf0205031] Letizia et al., 2007, Development 134(7): 1337--1346 Antagonistic and cooperative actions of the EGFR and Dpp pathways on the iroquois genes regulate Drosophila mesothorax specification and patterning. [FBrf0194844] Zeng et al., 2007, Development 134(11): 2061--2071 Drosophila Nemo antagonizes BMP signaling by phosphorylation of Mad and inhibition of its nuclear accumulation. [FBrf0201674] Takaesu et al., 2006, Genetics 174(3): 1299--1313 dSno facilitates baboon signaling in the Drosophila brain by switching the affinity of Medea away from Mad and toward dSmad2. [FBrf0193040] Moser and Campbell, 2005, Dev. Biol. 286(2): 647--658 Generating and interpreting the Brinker gradient in the Drosophila wing. [FBrf0190146] Takaesu et al., 2005, Development 132(21): 4883--4894 DNA-binding domain mutations in SMAD genes yield dominant-negative proteins or a neomorphic protein that can activate WG target genes in Drosophila. [FBrf0190324] Koh et al., 2004, Hum. Mol. Genet. 13(18): 2019--2030 A Drosophila model of early onset torsion dystonia suggests impairment in TGF-beta signaling. [FBrf0180338] McCabe et al., 2004, Neuron 41(6): 891--905 Highwire regulates presynaptic BMP signaling essential for synaptic growth. [FBrf0175022] Marquez et al., 2001, Genetics 157(4): 1639--1648 Transgenic analysis of the Smad family of TGF-beta signal transducers in Drosophila melanogaster suggests new roles and new interactions between family members. [FBrf0135803] Newfeld et al., 1997, Development 124(16): 3167--3176 Mothers against dpp participates in a DDP/TGF-beta responsive serine-threonine kinase signal transduction cascade. [FBrf0098317] Tsuneizumi et al., 1997, Nature 389(6651): 627--631 Daughters against dpp modulates dpp organizing activity in Drosophila wing development. [FBrf0098897] Newfeld et al., 1996, Development 122(7): 2099--2108 Mothers against dpp encodes a conserved cytoplasmic protein required in DPP/TGF-beta responsive cells. [FBrf0088659]

Allele Dmel\MadScer\UAS.cNa

Allele Dmel\Mad^Scer\UAS.cNa