Open Close
General Information
Symbol
Dmel\MadUAS.cNa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Newfeld
FlyBase ID
FBal0052172
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Mad, UAS.Mad
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
Expression of the complete Mad cDNA is governed by UASt regulatory sequences.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Overexpression of MadScer\UAS.cNa with Scer\GAL4A9 leads to a dramatic reduction in wing size, ectopic vein tissue and blistering.
Expression of MadScer\UAS.cNa in the wing discs under the control of Scer\GAL4T80 leads to wing vein truncation.
Expression of MadScer\UAS.cNa under the control of Scer\GAL4sca-537.4 results in wild-type wings 34% of the time, while the remaining wings have a variety of mild ectopic vein phenotypes.
Expression of MadScer\UAS.cNa using Scer\GAL4salm-459.2 has no severe effects on wing growth or patterning. Expression of MadScer\UAS.cNa using Scer\GAL4nub-AC-62 causes alterations in the vein pattern and wing size.
Overexpression of MadScer\UAS.cNa under the control of Scer\GAL4A9 results in wings of almost normal size that exhibit some ectopic vein tissue.
Expression of MadScer\UAS.cNa under the control of Scer\GAL4vg.int2.1 results in a broader wing and ectopic veins along L2 and L5 and emanating from the posterior crossvein.
Expression of MadScer\UAS.cNa under the control of Scer\GAL4ptc-559.1 produces excess vein tissue between L3/L4/L5 wing veins.
When expression is driven by Scer\GAL4ptc-559.1, ectopic vein tissue appears between wing veins L3 and L5. The distance between L3 and L5 appears reduced. Wings are 22% smaller than wild type. An ectopic leg develops on the ventral side of the normal limb. The ectopic leg is well formed with several segments and tarsal claws.
When expression is driven by Scer\GAL4vg.PM, outgrowth of wing tissue results.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
Statement
Reference
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Coexpression of fussScer\UAS.C in a MadScer\UAS.cNa, Scer\GAL4A9 background restores not only the size of the wing, but leads to a notable amelioration of the vein-intervein patterning.
Co-expression of sggS9A.Scer\UAS and MadScer\UAS.cNa under the control of Scer\GAL4sca-537.4 results in wings with no L1 or L3 sensilla, missing margin bristles of all types and occasional vein truncations. Co-expression of dshScer\UAS.cAa and MadScer\UAS.cNa under the control of Scer\GAL4sca-537.4 results in wings with no L1 or L3 sensilla, ectopic mechanosensory bristles on the dorsal margin and expansion of the L3 vein distally. Co-expression of sggA81T.Scer\UAS and MadScer\UAS.cNa under the control of Scer\GAL4sca-537.4 results in wings with ectopic sensilla on a slightly thickened L3 vein and ectopic mechanosensory bristles on the dorsal margin. Flies co-expressing AxnΔRGS.Scer\UAS and MadScer\UAS.cNa under the control of Scer\GAL4sca-537.4 have normal wing sensilla with occasional small gaps in the row or stout mechanosensory bristles on the margin.
Co-expression of MadScer\UAS.cNa suppresses the wing phenotypes caused by Scer\GAL4salm-459.2-mediated expression of SnooM67, giving rise to wings of almost normal size and pattern. Co-expression of MadScer\UAS.cNa and SnooM67 using Scer\GAL4nub-AC-62 results in wings with a normal pattern of veins. Scer\GAL4MD-638-mediated expression of the Snoo2c3 mutant via the P{GSV1} element associated with SnooM67 partially suppresses the wing phenotype caused by Scer\GAL4MD-638-mediated expression of Mad[Scer\UAS.cNa].
Co-expression of baboQ302D.Scer\UAS and MadScer\UAS.cNa, both under the control of Scer\GAL4A9, increases the transformation of intervein into vein tissue. Although the wing discs are larger, the adult wings are smaller due to cell death and transformation of intervein into more tightly associated vein cells. Co-expression of myoglianinScer\UAS.cGa with MadScer\UAS.cNa, both under the control of Scer\GAL4A9, represses the pattern defects caused by excess MadScer\UAS.cNa. Co-expression of ActβCM.Scer\UAS and MadScer\UAS.cNa, both under the control of Scer\GAL4A9 dramatically enhances the ectopic wing vein formation seen in MadScer\UAS.cNa mutants alone. Co-expression of dawScer\UAS.cGa and MadScer\UAS.cNa, under the control of Scer\GAL4A9, decreases the wing size to 88% without affecting ectopic vein formation. Male wing discs are smaller in size in these double mutants.
Co-expression of nmoScer\UAS.cUa suppresses the mutant wing phenotype caused by expression of MadScer\UAS.cNa under the control of Scer\GAL4vg.int2.1. rlSem/+ enhances the mutant wing phenotype caused by expression of MadScer\UAS.cNa under the control of Scer\GAL4vg.int2.1. Co-expression of rlSem.S.Scer\UAS enhances the mutant wing phenotype caused by expression of MadScer\UAS.cNa under the control of Scer\GAL4vg.int2.1.
Co-expression of MadScer\UAS.cNa partially rescues the phenotype caused by expression of SnooScer\UAS.cTa under the control of Scer\GAL4A9; wing size is rescued to wild type and the penetrance of the multiple wing vein defects is reduced to 19%.
DadScer\UAS.cTa suppresses the wing outgrowth phenotype of MadScer\UAS.cNa driven by Scer\GAL4vg.PM, resulting in an almost wild type wing.
Xenogenetic Interactions
Statement
Reference
Expression of MadScer\UAS.cNa in flies expressing Hsap\TOR1AΔE.Scer\UAS under the control of Scer\GAL4elav-C155 does not suppress the locomotor defects observed in flies expressing Hsap\TOR1AΔE.Scer\UAS alone.
Complementation and Rescue Data
Comments
Expression of MadScer\UAS.cNa under the control of Scer\GAL4elav.PLu in the neurons rescues neuromuscular junction size in Mad1/Df(2L)C28 mutants.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
MadScerUAS.cNa
MadScer\UAS.cNa
MadUAS.cNa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Newfeld
Secondary FlyBase IDs
    References (16)