FlyBase Allele Report: Dmel\E(spl)mδ-HLH[UAS.cdCa]

General Information

Symbol

Dmel\E(spl)mδ-HLH^UAS.cdCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of de Celis

FlyBase ID

FBal0052338

Feature type

allele

Associated gene

Dmel\E(spl)mδ-HLH

Associated Insertion(s)

Carried in Construct

P{UAS-mδ}

Also Known As

UAS-mδ, UAS-E(spl)mδ

Key Links

Transgenic product class

wild_type

(de Celis et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(de Celis et al., 1996)

Mutagen

in vitro construct

(Ligoxygakis et al., 1998, de Celis et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-mδ}

Cytology

Description

Expression of E(spl)mδ-HLH is governed by UAS regulatory sequences.

(de Celis et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

E(spl)mδ-HLH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^Mef2.PU

(Engel and Rand, 2014)

lethal - all die before end of P-stage, with Scer\GAL4^h-H10

(Ligoxygakis et al., 1998)

some die during pharate adult stage, with Scer\GAL4^h-H10

(Ligoxygakis et al., 1998)

visible, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^455.2

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^dpp.blk1

(Li et al., 2009)

visible, with Scer\GAL4^h-1J3

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^h-H10

(Ligoxygakis et al., 1998)

visible, with Scer\GAL4^pnr-MD237

(Giagtzoglou et al., 2003)

visible, with Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

visible, with Scer\GAL4^sca-537.4

(Nagel et al., 2000)

Phenotype Manifest In

adult thorax, with Scer\GAL4^pnr-MD237

(Giagtzoglou et al., 2003)

embryonic somatic muscle cell, with Scer\GAL4^Mef2.PU

(Engel and Rand, 2014)

eye, with Scer\GAL4^h-H10

(Ligoxygakis et al., 1998)

eye, with Scer\GAL4^sca-109-68

(Li et al., 2003)

larval segmental nerve branch SNa of A1-7, with Scer\GAL4^Mef2.PU

(Engel and Rand, 2014)

macrochaeta, with Scer\GAL4^pnr-MD237

(Giagtzoglou et al., 2003)

macrochaeta, with Scer\GAL4^sca-537.4

(Nagel et al., 2000)

microchaeta, with Scer\GAL4^pnr-MD237

(Giagtzoglou et al., 2003)

ommatidium, with Scer\GAL4^h-H10

(Ligoxygakis et al., 1998)

photoreceptor cell R1, with Scer\GAL4^hs.2sev

(Bhattacharya and Baker, 2009)

photoreceptor cell R6, with Scer\GAL4^hs.2sev

(Bhattacharya and Baker, 2009)

photoreceptor cell R7 | ectopic, with Scer\GAL4^hs.2sev

(Bhattacharya and Baker, 2009)

photoreceptor cell R8, with Scer\GAL4^sca-109-68

(Li et al., 2003)

scutellar bristle, with Scer\GAL4^455.2

(Ligoxygakis et al., 1999)

scutellar bristle, with Scer\GAL4^dpp.blk1

(Li et al., 2009)

scutellar bristle, with Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

scutum & macrochaeta, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

scutum & macrochaeta, with Scer\GAL4^h-1J3

(Ligoxygakis et al., 1999)

scutum & microchaeta, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

scutum & microchaeta, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

wing margin bristle, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

wing vein, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

Detailed Description

Statement

Reference

Expression of E(spl)mδ-HLH^{Scer\UAS.cdCa} in the somatic musculature, under the control of Scer\GAL4^Mef2.PU causes a significant failure in SNa branching.

Expression of E(spl)mδ-HLH^{Scer\UAS.cdCa} in neurons, under the control of Scer\GAL4^elav-C155, does not affect SNa branching.

Embryos expressing E(spl)mδ-HLH^{Scer\UAS.cdCa} in the somatic musculature, under the control of Scer\GAL4^Mef2.PU show higher rates of muscle pattern failure, demonstrating a disordered muscle phenotype in 58% of hemisegments.

(Engel and Rand, 2014)

No extra neuroblasts are seen when E(spl)mδ-HLH^{Scer\UAS.cdCa} is expressed under the control of Scer\GAL4^wor.PA or Scer\GAL4^elav.PU.

(San-Juán and Baonza, 2011)

In 14% of ommatidia expressing Scer\GAL4^hs.2sev driven HLHmδ^{Scer\UAS.cdCa}, some of the R1/6 precursors develop as R7-like cells.

(Bhattacharya and Baker, 2009)

Flies expressing HLHmδ^{Scer\UAS.cdCa} under the control of Scer\GAL4^dpp.blk1 have missing scutellar bristles.

(Li et al., 2009)

Expression of HLHmδ^{Scer\UAS.cdCa} under the control of Scer\GAL4^pnr-MD237 inhibits the formation of both macro- and micro-chaetae, resulting in a bald stripe in the centre of the adult thorax.

(Giagtzoglou et al., 2003)

When HLHmδ^{Scer\UAS.cdCa} is driven by Scer\GAL4^sca-109-68 R8 photoreceptor cells are sometimes missing. Adult eyes are small and rough.

(Li et al., 2003)

Expression of HLHmδ^{Scer\UAS.cdCa} under the control of Scer\GAL4^ey.PB has no effect on either eye or antenna disc development.

(Kumar and Moses, 2001)

When HLHmδ^{Scer\UAS.cdCa} is driven by Scer\GAL4^sca-537.4 bristle los is observed on the notum.

(Nagel et al., 2000)

Scutellar bristles are completely suppressed in flies with HLHmδ^{Scer\UAS.cdCa} driven by Scer\GAL4^ptc-559.1. Scutellar bristles are completely suppressed in flies with HLHmδ^{Scer\UAS.cdCa} driven by Scer\GAL4^455.2.

(Apidianakis et al., 1999)

Flies expressing HLHmδ^{Scer\UAS.cdCa} under the control of Scer\GAL4^32B show loss of wing veins. Loss of notum microchaetae and wing margin bristles is seen. Flies expressing HLHmδ^{Scer\UAS.cdCa} under the control of Scer\GAL4^ap-md544 show loss of notum microchaetae and macrochaetae, except for a few central microchaetae and at the very anterior edge of the notum. Flies expressing HLHmδ^{Scer\UAS.cdCa} under the control of Scer\GAL4^455.2 show severe loss of scutellar bristles. Flies expressing HLHmδ^{Scer\UAS.cdCa} under the control of Scer\GAL4^h-1J3 show loss of notum macrochaetae.

(Ligoxygakis et al., 1999)

Flies expressing two copies of HLHmδ^{Scer\UAS.cdCa} under the control of two copies of Scer\GAL4^h-H10 have much smaller eyes than normal which contain a few facets. Eye imaginal discs contain few ommatidia. These animals survive to pharate adulthood, occasionally producing adult escapers. Flies expressing one copy of HLHmβ^{Scer\UAS.cdCa} under the control of one copy of Scer\GAL4^h-H10 have a mild rough eye phenotype.

(Ligoxygakis et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

E(spl)mδ-HLH^UAS.cdCa, Scer\GAL4^ptc-559.1 has visible phenotype, non-suppressible by E(spl)m4-BFM^UAS.cAa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

E(spl)mδ-HLH^UAS.cdCa, Scer\GAL4^ptc-559.1 has visible phenotype, non-suppressible by E(spl)mα-BFM^UAS.cAa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

Enhancer of

Statement

Reference

Scer\GAL4^32B/E(spl)mδ-HLH^UAS.cdCa is an enhancer of visible | recessive | heat sensitive phenotype of N^l1N-ts1

(Ligoxygakis et al., 1999)

Suppressor of

Statement

Reference

Scer\GAL4^32B/E(spl)mδ-HLH^UAS.cdCa is a suppressor of visible | recessive | heat sensitive phenotype of N^l1N-ts1

(Ligoxygakis et al., 1999)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

E(spl)mδ-HLH^UAS.cdCa, Scer\GAL4^ptc-559.1 has scutellar bristle phenotype, non-suppressible by E(spl)m4-BFM^UAS.cAa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

E(spl)mδ-HLH^UAS.cdCa, Scer\GAL4^ptc-559.1 has scutellar bristle phenotype, non-suppressible by E(spl)mα-BFM^UAS.cAa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

Enhancer of

Statement

Reference

Scer\GAL4^32B/E(spl)mδ-HLH^UAS.cdCa is an enhancer of wing | heat sensitive phenotype of N^l1N-ts1

(Ligoxygakis et al., 1999)

Suppressor of

Statement

Reference

E(spl)mδ-HLH^UAS.cdCa is a suppressor of macrochaeta & wing | ectopic phenotype of Mmus\Neurog1^UAS.cQa, Scer\GAL4^dpp.blk1

(Quan et al., 2004)

E(spl)mδ-HLH^UAS.cdCa, Scer\GAL4^pnr-MD237 is a suppressor | partially of chaeta | ectopic phenotype of Scer\GAL4^pnr-MD237, sc^UAS.cPa

(Giagtzoglou et al., 2003)

Scer\GAL4^32B/E(spl)mδ-HLH^UAS.cdCa is a suppressor of wing | heat sensitive phenotype of N^l1N-ts1

(Ligoxygakis et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of HLHmδ^{Scer\UAS.cdCa} partially suppresses the ectopic chaetae phenotype caused by expression of sc^Scer\UAS.cPa under the control of Scer\GAL4^pnr-MD237.

(Giagtzoglou et al., 2003)

HLHmδ^{Scer\UAS.cdCa} expressed under the control of Scer\GAL4^32B restores the distal wing margin and adjacent wing blade tissue that is missing in Df(1)N-54l9 heterozygotes. Expression of HLHmδ^{Scer\UAS.cdCa} under the control of Scer\GAL4^32B enhances the scalloped wing phenotype seen in hemizygous N^l1N-ts1 flies shifted to the non-permissive temperature at the early third larval instar stage (72-84 hours after egg laying). Expression of HLHmδ^{Scer\UAS.cdCa} under the control of Scer\GAL4^32B suppresses the nicked wing phenotype seen in hemizygous N^l1N-ts1 flies shifted to the non-permissive temperature at the mid third larval instar stage (96-108 hours after egg laying).

(Ligoxygakis et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26677

y¹ w^*; P{UAS-mδ}h8

Notes on Origin

Discoverer

Comments

The relative ability of UAS constructs of the E(spl) complex to induce wing vein deletion when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): HLHmβ^{Scer\UAS.cdCa} = HLHmγ^Scer\UAS.cLa > E(spl)^Scer\UAS.cNa = HLHm7^{Scer\UAS.cdCa} > HLHmδ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of notum microchaetae when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): E(spl)^Scer\UAS.cNa > HLHm7^{Scer\UAS.cdCa} >> HLHmγ^Scer\UAS.cLa = HLHmβ^{Scer\UAS.cdCa} = HLHm3^Scer\UAS.cLa > HLHmδ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of wing margin bristles when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): HLHm7^{Scer\UAS.cdCa} > E(spl)^Scer\UAS.cNa > HLHmγ^Scer\UAS.cLa > HLHmδ^{Scer\UAS.cdCa} > HLHmβ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of notum macrochaetae when expressed under the control of Scer\GAL4^h-1J3 is (ranked according to severity of phenotype): E(spl)^Scer\UAS.cNa > HLHm7^{Scer\UAS.cdCa} > HLHmβ^{Scer\UAS.cdCa} > HLHmδ^{Scer\UAS.cdCa} > HLHmγ^Scer\UAS.cLa = HLHm3^Scer\UAS.cLa.

(Ligoxygakis et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

E(spl)mδ-HLH^{Scer\UAS.cdCa}

E(spl)mδ-HLH^UAS.cdCa

HLHmδ^{Scer\UAS.cdCa}

HLHmδ^UAS.cdCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of de Celis

(de Celis et al., 1996)

Secondary FlyBase IDs

References (22)

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