Open Close
General Information
Symbol
Ecol\lacZkek1-15A6
Species
E. coli
Name
FlyBase ID
FBal0055567
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
kek1-lacZ, 15A6, kek-lacZ
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
P{lArB}kek115A6
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
egg chamber | restricted

Comment: at anterior and posterior poles

Additional Information
Statement
Reference

In the wing pouch, Ecol\lacZkek1-15A6 is expressed as a broad stripe straddling the A/P boundary in the L3 and L4 intervein region and also in the hinge and the notum. Ecol\lacZkek1-15A6 is expressed in the presumptive hinge of the haltere pouch.

Expression of the kek1 lacZ reporter which mimicks endogenous transcript expression in the embryo is detected in the vein primordia and the L3/L4 intervein region in the wing disc. kek1-lacZ is also detected posterior to the morphogenetic furrow in the developing eye disc.

Enhancer trap expression is first detected at stage 10 in a few cells per segment in the dorsal midline of the CNS. By early stage 12, the midline staining has evolved to a ventral position. The stained midline cells are thought to include the VUM neurons. By stage 12, expression is seem in many CNS neurons and is maintained through larval development. Expression is also seen in a small subset of ventral and lateral cell clusters of the PNS and in the cap cells in the abdominal segments. In the head region, expression is seen in both neural and nonneuronal cells of the antenno-maxillary complex. Enhancer trap expression was observed in larvae in all imaginal disc while they are undergoing patterning. In the eye-antennal disc, expression is found in the differentiating retinal epithelium, from the posterior border up to several rows behind the morphogenetic furrow, as well as in the presumptive ocelli and ocellar bristles. Dynamic expression is observed during patterning of the follicle cell epithelium in the ovary. During oogenesis, weak expression in nurse cells and stronger expression in columnar follicle cells surrounding the oocyte is seen. By stage S7, expression is apparent in follicle cells at the posterior pole surrounding the oocyte. By stage 9, the posterior expression has disappeared and expression is strong in follicle cells above the oocyte nucleus. This pattern reflects the onset of dorsoventral polarity in the egg chamber and at stage S9 the expression occurs in a gradient. Expression is also seen in border follicle cells.

 
Marker for
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Ecol\lacZkek1-15A6
lacZkek1-15A6
Name Synonyms
Secondary FlyBase IDs
    References (12)