5.2kb y gene fragment containing 2.8kb of 5' flanking and 0.13kb of 3' flanking DNA and lacking the intron that is the location of the tissue-specific bristle and tarsal claw enhancers.
Expression of y+mDint2 is reduced (in a background of reduced su(Hw)+) by the expression of tethered Pc, Scm or esc. In the case of Pchs.T:su(Hw)-ZnF,T:Zzzz\FLAG, the effect is seen without the use of heat shock. For Scmhs.T:su(Hw)-ZnF,T:Zzzz\FLAG and eschs.T:su(Hw)-ZnF,T:Zzzz\FLAG heat shock must be applied daily. Expression in the body cuticle is less sensitive to repression than in the wing cuticle.