FlyBase Allele Report: Dmel\wun[k10201]

General Information

Symbol

Dmel\wun^k10201

Species

D. melanogaster

Name

FlyBase ID

FBal0055780

Feature type

allele

Associated gene

Dmel\wun

Associated Insertion(s)

P{lacW}wun^k10201

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Zhang et al., 1997)

Mutagen

P-element activity

(Zhang et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{lacW}wun^k10201

Cytology

Description

P{lacW} is inserted into the l(2)k10201 transcription unit, approximately 2kb downstream of the wun transcription unit, and transcribed in the same direction as l(2)k10201, thus the opposite direction to wun. The product of l(2)k10201 is truncated and the expression of wun is down-regulated.

(Zhang et al., 1997)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Duchenne muscular dystrophy

modeled by Dys^RNAi.NH2.UAS

(Pantoja et al., 2013)

modeled by Dys^RNAi.C.UAS

(Pantoja et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Manifest In

germline cell | female

(Zhang et al., 1997)

germline cell | male

(Zhang et al., 1997)

Detailed Description

Statement

Reference

Germ cells are not normally found in the distal part of the gut, though they are in wun mutants. Germ cells migrate largely at random. Neuronal migration is unaffected.

(Zhang et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

wun^k10201, Scer\GAL4^Act.PU is a suppressor of abnormal locomotor behavior phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Pantoja et al., 2013)

wun[+]/wun^k10201 is a suppressor of abnormal locomotor behavior phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Pantoja et al., 2013)

wun[+]/wun^k10201 is a suppressor of visible phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

wun[+]/wun^k10201 is a suppressor of visible phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Phenotype Manifest In

Suppressor of

Statement

Reference

wun[+]/wun^k10201 is a suppressor of indirect flight muscle cell phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Pantoja et al., 2013)

wun[+]/wun^k10201 is a suppressor of myofibril phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Pantoja et al., 2013)

wun[+]/wun^k10201 is a suppressor of posterior crossvein phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

wun[+]/wun^k10201 is a suppressor of posterior crossvein phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Other

Statement

Reference

Dys^RNAi.C.UAS, wun[+]/wun^k10201 has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of wun^k10201 suppresses the gross defects in indirect flight muscle morphology seen when Dys^{dsRNA.C.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU. The reduction in activity levels is also suppressed.

One copy of wun^k10201 suppresses the defects in indirect flight muscle myofibril integrity seen in Df(3R)Exel6184 mutant flies.

One copy of wun^k10201 suppresses the reduction in activity levels seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

(Pantoja et al., 2013)

One copy of wun^k10201 weakly suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.C.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU but produces extra wing vein material.

One copy of wun^k10201 suppresses the posterior crossvein phenotype seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

(Kucherenko et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

wun^EMS23

(Kozopas, 2003.3)

wun^EMS41

(Kozopas, 2003.3)

wun^EMS4