FlyBase Allele Report: Dmel\wg[UAS.cAa]

General Information

Symbol

Dmel\wg^UAS.cAa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Azpiazu

FlyBase ID

FBal0055792

Feature type

allele

Associated gene

Dmel\wg

Associated Insertion(s)

Carried in Construct

P{UAS-wg.A}

Also Known As

UAS-wg

Key Links

Transgenic product class

cDNA

(Azpiazu et al., 1996)

wild_type

(Azpiazu et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Azpiazu et al., 1996)

wild_type

(Azpiazu et al., 1996)

Mutagen

in vitro construct

(Hazelett et al., 1998, Azpiazu et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-wg.A}

Cytology

Description

UASt regulatory sequences drive expression of the wg cDNA c14.

(Azpiazu et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Singh et al., 2006)

eye disc, with Scer\GAL4^ey.PH

(Singh et al., 2006)

eye disc | posterior, with Scer\GAL4^ey.PH

(Hazelett et al., 1998)

eye disc morphogenetic furrow | cell non-autonomous | somatic clone, with Scer\GAL4^Act5C.PP

(Lee and Treisman, 2001)

photoreceptor neuron, with Scer\GAL4^ey.PH

(Hazelett et al., 1998)

Detailed Description

Statement

Reference

Expression of wg^Scer\UAS.cAa under the control of Scer\GAL4^ey.PH results in small eyes both at the level of adult eyes and in the eye disc.

(Singh et al., 2006)

Expression of wg^Scer\UAS.cAa under the control of Scer\GAL4^Act5C.PP in clones in the posterior of the eye disc can result in nonautonomous stalling of the morphogenetic furrow.

(Lee and Treisman, 2001)

Flies expressing wg^Scer\UAS.cAa under the control of Scer\GAL4^ey.PH do not show photoreceptor development at the posterior of the eye disc.

(Hazelett et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ey.PH, wg^UAS.cAa has eye phenotype, enhanceable by L²/L[+]

(Singh et al., 2006)

Scer\GAL4^ey.PH, wg^UAS.cAa has eye disc phenotype, enhanceable by L²/L[+]

(Singh et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, wg^UAS.cAa has photoreceptor neuron phenotype, suppressible | partially by spi^s.UAS/Scer\GAL4^ey.PH

(Hazelett et al., 1998)

Scer\GAL4^ey.PH, wg^UAS.cAa has eye disc | posterior phenotype, suppressible | partially by spi^s.UAS/Scer\GAL4^ey.PH

(Hazelett et al., 1998)

Scer\GAL4^ey.PH, wg^UAS.cAa has photoreceptor neuron phenotype, suppressible by Ras85D^V12.UAS, Scer\GAL4^ey.PH

(Hazelett et al., 1998)

Scer\GAL4^ey.PH, wg^UAS.cAa has eye disc | posterior phenotype, suppressible by Ras85D^V12.UAS, Scer\GAL4^ey.PH

(Hazelett et al., 1998)

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PH, wg^UAS.cAa has photoreceptor neuron phenotype, non-suppressible by dpp^UAS.cFa/Scer\GAL4^ey.PH

(Hazelett et al., 1998)

Scer\GAL4^ey.PH, wg^UAS.cAa has eye disc | posterior phenotype, non-suppressible by dpp^UAS.cFa/Scer\GAL4^ey.PH

(Hazelett et al., 1998)

Scer\GAL4^ey.PH, wg^UAS.cAa has photoreceptor neuron phenotype, non-suppressible by tkv^{Q253D.UAS.cNb}/Scer\GAL4^ey.PH

(Hazelett et al., 1998)

Scer\GAL4^ey.PH, wg^UAS.cAa has eye disc | posterior phenotype, non-suppressible by tkv^{Q253D.UAS.cNb}/Scer\GAL4^ey.PH

(Hazelett et al., 1998)

Enhancer of

Statement

Reference

Scer\GAL4^ey.PH/wg^UAS.cAa is an enhancer of eye disc | ventral phenotype of L²

(Singh et al., 2006)

Scer\GAL4^ey.PH/wg^UAS.cAa is an enhancer of eye | ventral phenotype of L²

(Singh et al., 2006)

Suppressor of

Statement

Reference

Scer\GAL4^ey.PH/wg^UAS.cAa is a suppressor of eye disc | somatic clone phenotype of pnr^VX6

(Maurel-Zaffran and Treisman, 2000)

Scer\GAL4^ey.PH/wg^UAS.cAa is a suppressor of photoreceptor neuron | ectopic | somatic clone phenotype of pnr^VX6

(Maurel-Zaffran and Treisman, 2000)

NOT Suppressor of

Statement

Reference

Scer\GAL4^twi.PG/wg^UAS.cAa is a non-suppressor of embryonic/larval dorsal vessel & embryonic myoblast phenotype of slp1^Δ34B, slp2^Δ34B

(Lee and Frasch, 2000)

Scer\GAL4^twi.PG/wg^UAS.cAa is a non-suppressor of embryonic/larval somatic muscle & embryonic myoblast | dorsal phenotype of slp1^Δ34B, slp2^Δ34B

(Lee and Frasch, 2000)

Scer\GAL4^ey.PH/wg^UAS.cAa is a non-suppressor of photoreceptor neuron | ectopic phenotype of Scer\GAL4^ey.PH, dpp^UAS.cFa

(Hazelett et al., 1998)

Scer\GAL4^ey.PH/wg^UAS.cAa is a non-suppressor of eye disc | anterior phenotype of Scer\GAL4^ey.PH, dpp^UAS.cFa

(Hazelett et al., 1998)

Other

Statement

Reference

Delta::N^ΔECN.UAS, Scer\GAL4^ey.PH, wg^UAS.cAa has eye disc phenotype

(Maurel-Zaffran and Treisman, 2000)

Delta::N^ΔECN.UAS, Scer\GAL4^ey.PH, wg^UAS.cAa has photoreceptor neuron phenotype

(Maurel-Zaffran and Treisman, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of either wg^Scer\UAS.cAa, under the control of Scer\GAL4^ey.PH, in L²/+ mutants results in a no eye phenotype, while small eyes are observed in both of the single mutants.

(Singh et al., 2006)

Coexpression of both wg^Scer\UAS.cAa and tsh^Scer\UAS.cGa in clones under the control of Scer\GAL4^Act5C.PI results in eye suppression in the dorsal eye disc. Coexpression of both wg^Scer\UAS.cAa and tsh^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 results in suppression of eye fate at the dorsal and ventral margins of the eye.

(Singh et al., 2002)

Flies expressing both wg^Scer\UAS.cAa and Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^ey.PH show photoreceptor differentiation initiating all around the margins in the eye disc. Large pnr^VX6 clones in the eye, which would be expected to produce an ectopic eye field in a wild-type background, show no photoreceptor differentiation in discs if they are also expressing wg^Scer\UAS.cAa under the control of Scer\GAL4^ey.PH.

(Maurel-Zaffran and Treisman, 2000)

The lack of photoreceptor development seen in flies expressing wg^Scer\UAS.cAa under the control of Scer\GAL4^ey.PH is not rescued if the flies are also co-expressing dpp^Scer\UAS.cFa or tkv^{Q253D.Scer\UAS.cNb}. Flies expressing both dpp^Scer\UAS.cFa and wg^Scer\UAS.cAa under the control of Scer\GAL4^Act5C.PP also do not show photoreceptor development at the posterior margin of the eye disc. Photoreceptor differentiation and growth is rescued in flies expressing wg^Scer\UAS.cAa under the control of Scer\GAL4^ey.PH if they are also co-expressing Ras85D^V12.Scer\UAS (strong rescue) or spi^s.Scer\UAS (weak rescue). Flies expressing dpp^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH show induction of photoreceptor differentiation from the anterior margin of the eye disc. This ectopic differentiation is not inhibited if the flies are also co-expressing wg^Scer\UAS.cAa.

(Hazelett et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

wg^Scer\UAS.cAa

wg^UAS.cAa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Azpiazu

(Azpiazu et al., 1996)

Secondary FlyBase IDs

References (10)

Report Sections

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