FlyBase Allele Report: Dmel\rho[UAS.cGa]

General Information

Symbol

Dmel\rho^UAS.cGa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Golembo

FlyBase ID

FBal0055945

Feature type

allele

Associated gene

Dmel\rho

Associated Insertion(s)

Carried in Construct

P{UAS-ve.(rho)G}

Also Known As

UAS-rho

Key Links

Transgenic product class

cDNA

(Golembo et al., 1996)

wild_type

(Golembo et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Golembo et al., 1996)

wild_type

(Golembo et al., 1996)

Mutagen

in vitro construct

(Golembo et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-ve.(rho)G}

Cytology

Description

UASt regulatory sequences drive expression of a 1.05kb DdeI cDNA fragment containing the entire rho coding sequence.

(Golembo et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

rho

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

chordotonal organ precursor cell & ventral thoracic disc, with Scer\GAL4^sca-109-68

(zur Lage and Jarman, 1999)

denticle | ectopic, with Scer\GAL4^en-e16E

(O'Keefe et al., 1997)

dorsal appendage | cell non-autonomous | ectopic | somatic clone, with Scer\GAL4^Act5C.PP

(Ward and Berg, 2005)

dorsal appendage | ectopic, with Scer\GAL4^55B

(Sapir et al., 1998)

dorsal appendage | ectopic | somatic clone, with Scer\GAL4^Act5C.PP

(Ward and Berg, 2005)

egg chorion, with Scer\GAL4^T155

(Ghiglione et al., 1999)

egg chorion | ectopic | somatic clone, with Scer\GAL4^Act5C.PP

(Ward and Berg, 2005)

embryonic/first instar larval cuticle, with Scer\GAL4^en-e16E

(O'Keefe et al., 1997)

larval dorsal hair, with Scer\GAL4^55B

(Sapir et al., 1998)

midline glial cell, with Scer\GAL4^elav.PLu

(Bergmann et al., 2002)

ventral denticle belt, with Scer\GAL4^55B

(Sapir et al., 1998)

wing disc | somatic clone, with Scer\GAL4^αTub84B.PZ

(Zecca and Struhl, 2002)

wing disc dorsal compartment | ectopic | somatic clone, with Scer\GAL4^αTub84B.PZ

(Zecca and Struhl, 2002)

wing disc dorsal compartment | somatic clone, with Scer\GAL4^αTub84B.PZ

(Zecca and Struhl, 2002)

Detailed Description

Statement

Reference

Somatic clones of rho^Scer\UAS.cGa; Scer\GAL4^Act5C.PP cells in the follicle cells produce either ectopic dorsal appendages or ectopic chorionic material resembling warts. These warts are restricted to the the ventral/lateral region in the middle of the egg chamber, whereas ectopic dorsal appendages only form in the ventral/lateral region around the anterior circumference of the egg chamber (the collar region). These ectopic appendages include many cells recruited by the clone - clones of just 2 rho^Scer\UAS.cGa; Scer\GAL4^Act5C.PP cells can induce ectopic appenadge formation.

(Ward and Berg, 2005)

rho^Scer\UAS.cGa; Scer\GAL4^elav.PLu embryos have increased numbers of midline glial cells.

(Bergmann et al., 2002)

Clones in the prospective notum or wing hinge in the wing disc expressing rho^Scer\UAS.cGa under the control of Scer\GAL4^αTub84B.PZ have wiggly borders.

(Zecca and Struhl, 2002)

Clones in the wing disc (generated during the first larval instar) expressing rho^Scer\UAS.cGa under the control of Scer\GAL4^αTub84B.PZ can result in dramatic reorganisation of the wing disc, including the formation of ectopic dorsal compartments and ectopic notum primordia or the abnormal expansion of the dorsal compartment.

(Zecca and Struhl, 2002)

Ectopic expression of rho^Scer\UAS.cGa under the control of Scer\GAL4^E4 in a wild-type background has no visible effect on chorion structure.

(Peri and Roth, 2000)

Eggs derived from females expressing rho^Scer\UAS.cGa under the control of Scer\GAL4^T155 are dorsalised.

(Ghiglione et al., 1999)

Leg discs in which rho^Scer\UAS.cGa is expressed under the control of Scer\GAL4^sca-109-68 have an increased number of sensory organ precursor cells in the chordotonal cluster. The proneural cluster in the ectoderm is reduced or absent and the invaginating stalk of cells is much thicker than wild type.

(zur Lage and Jarman, 1999)

Scer\GAL4^55B-mediated expression causes the production of dorsal appendages around the anterior circumference of the egg. A small fraction of the eggs are fertilised and develop into dorsalised embryos (absence or complete elimination of denticle bands and expansion of dorsal hairs).

(Sapir et al., 1998)

Embryos expressing rho^Scer\UAS.cGa under the control of Scer\GAL4^en-e16E have ectopic denticles anterior to the denticle belts.

(O'Keefe et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^αTub84B.PZ/rho^UAS.cGa is a suppressor | cell non-autonomous | somatic clone of decreased occurrence of cell division | larval stage phenotype of vn^L6/vn^ddd-4

(Zecca and Struhl, 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Act5C.PP, rho^UAS.cGa, spi^s.UAS has eye photoreceptor cell | somatic clone phenotype, suppressible by rasp^T392/rasp^T802

(Miura et al., 2006)

Scer\GAL4^Act5C.PP, rho^UAS.cGa, spi^s.UAS.EGFP has eye photoreceptor cell | somatic clone phenotype, suppressible by rasp^T392/rasp^T802

(Miura et al., 2006)

Scer\GAL4^55B, rho^UAS.cGa has dorsal appendage phenotype, suppressible by S^IIN

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has ventral denticle belt phenotype, suppressible by S^IIN

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has larval dorsal hair phenotype, suppressible by S^IIN

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has dorsal appendage phenotype, suppressible | partially by grk³

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has ventral denticle belt phenotype, suppressible | partially by grk³

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has larval dorsal hair phenotype, suppressible | partially by grk³

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has dorsal appendage phenotype, suppressible | partially by grk⁶

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has ventral denticle belt phenotype, suppressible | partially by grk⁶

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has larval dorsal hair phenotype, suppressible | partially by grk⁶

(Sapir et al., 1998)

Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/rho^UAS.cGa is a suppressor of denticle row 4 phenotype of Ser^RX82

(Walters et al., 2005)

Scer\GAL4^αTub84B.PZ/rho^UAS.cGa is a suppressor | partially of wing disc phenotype of vn^L6/vn^ddd-4

(Zecca and Struhl, 2002)

Other

Statement

Reference

Scer\GAL4^Act5C.PP, rho^UAS.cGa, spi^s.UAS has eye photoreceptor cell | somatic clone phenotype

(Miura et al., 2006)

Scer\GAL4^Act5C.PP, rho^UAS.cGa, spi^s.UAS.EGFP has eye photoreceptor cell | somatic clone phenotype

(Miura et al., 2006)

Scer\GAL4^ptc-559.1, Ser^RX82, rho^UAS.cGa has denticle row 5 phenotype

(Walters et al., 2005)

S^UAS.cBa, Scer\GAL4^hs.PB, rho^UAS.cGa has bract | ectopic phenotype

(del Alamo et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Somatic clones of Scer\GAL4^Act5C.PP cells in the eye disc in animals carrying rho^Scer\UAS.cGa and spi^s.Scer\UAS or spi^{Scer\UAS.s.T:Avic\GFP} cause ectopic photoreceptor differentiation. This phenotype is completely suppressed by rasp^T392/rasp^T802.

(Miura et al., 2006)

Expression of rho^Scer\UAS.cGa, under the control of Scer\GAL4^ptc-559.1, in Ser^RX82 embryos raised at 18 or 22^oC rarely restores proper denticle row 4 polarity. However, raising embryos of the same genotype at 29^oC does restore row 4 polarity and causes an expansion of type 5 denticles posterior to the normal denticle field.

(Walters et al., 2005)

Clones expressing rho^Scer\UAS.cGa under the control of Scer\GAL4^αTub84B.PZ induced in the first larval instar can stimulate proliferation in vn^L6/vn^ddd-4 wing discs, irrespective of the position of the clone within the wing disc.

(Zecca and Struhl, 2002)

When rho^Scer\UAS.cBa and S^Scer\UAS.cBa are driven by Scer\GAL4^hs.PB and heatshocked, extra bracts are seen to associate with a few of the mechanosensory bristles, but not chemosensory bristles.

(del Alamo et al., 2002)

The ventralised phenotype of eggs derived from females expressing kek1^Scer\UAS.cGa under the control of Scer\GAL4^T155 is not altered if the females are also coexpressing rho^Scer\UAS.cGa.

(Ghiglione et al., 1999)

Leg discs in which both rho^Scer\UAS.cGa and Dl::N^{ΔECN.Scer\UAS} are expressed under the control of Scer\GAL4^sca-109-68 have a phenotype resembling that of rho^Scer\UAS.cGa expressed under the control of Scer\GAL4^sca-109-68 rather than that of Dl::N^{ΔECN.Scer\UAS} expressed under the control of Scer\GAL4^sca-109-68.

(zur Lage and Jarman, 1999)

S^IIN mutation dramatically suppresses the dorsal appendage phenotype, appendages appear more normal and the embryos displays reduced but distinct ventral denticle belts. spi mutation has no effect on the dorsal appendage phenotype. Expression in grk mutants reduced the severity of the phenotype, some ectopic dorsal appendage material is produced and embryos form denticle belts.

(Sapir et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^rho.654/rho^UAS.cGa partially rescues rho^7M43

(Li-Kroeger et al., 2008)

Comments

rho^Scer\UAS.cGa driven by Scer\GAL4^rho.654 rescues the oenocyte defect but not the secondary sensory organ precursor cell defects associated with rho^7M43.

(Li-Kroeger et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

rho^Scer\UAS.cGa

rho^UAS.cGa

ve^Scer\UAS.cGa

(Sapir et al., 1998)

ve^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Golembo

(Golembo et al., 1996)

Secondary FlyBase IDs

References (19)

Report Sections

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