Open Close
General Information
Symbol
Dmel\shgUAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Sanson
FlyBase ID
FBal0056179
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-DE-cadherin, UAS-DE-cad, UAS-CAD, UAS-DECad
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of a full-length shg cDNA.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
model of  cancer
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
DOES NOT ameliorate  cancer
model of  cancer
is NOT ameliorated by biUAS.cBa
is NOT ameliorated by biUAS.cPa
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Overexpression of shgScer\UAS.cSa 6-fold in nurse cells (under the control of Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG, and Scer\GAL4nos.UTR.T:Hsim\VP16) results in border cells migrating along a normal trajectory more slowly than normal and exhibiting a hyperpolarized morphology. A total of 86% of protrusions from border cell clusters migrating between nurse cells are directed toward the oocyte (compared to 76% in wild-type). Overexpression of shg in outer, migratory border cells using Scer\GAL4slbo.2.6 or in polar cell using Scer\GAL4upd.PU does not affect border cell migration significantly.
The expression of shgScer\UAS.cSa under the control of Scer\GAL4dpp.PU induces long distance cell migration and scattering, even across the anterior-posterior compartment border, in third instar larval wing discs, as compared to controls.
Expression of shgScer\UAS.cSa, under the control of Scer\GAL4e22c, results in clones that are apically constricted. Somatic clones overexpressing shgScer\UAS.cSa under the control of Scer\GAL4vg.PM, separate from their neighbours and display smooth clone borders.
Expression of shgScer\UAS.cSa under the control of Scer\GAL4hs.2sev in ommatidia at the time of rotation has no effect on eyes when expressed at moderate levels and causes epithelial defects at high levels. The ommatidial under-rotation of Scer\GAL4hs.2sev>shgdCR3h.Scer\UAS.T:Avic\GFP-rs eyes is suppressed in Scer\GAL4hs.2sev>shgdCR3h.Scer\UAS.T:Avic\GFP-rs; Scer\GAL4hs.2sev>shgScer\UAS.cSa flies. Expression of shgScer\UAS.cSa under the control of Scer\GAL4hs.2sev increases the number of ommatidial clusters that initiate rotation correctly in shgk03401 clones.
Clones of follicle cells that are expressing shgScer\UAS.cSa under the control of Scer\GAL4Act5C.PP can cause mislocalisation of the oocyte, and in these cases the mislocalised oocyte is always in contact with a cell or group of cells expressing shgScer\UAS.cSa under the control of Scer\GAL4Act5C.PP.
Expression of shgScer\UAS.cSa, under the control of Scer\GAL4elav-C155, does not suppress the R cell axon extension phenotype of Df(2L)CadNΔ14 clones.
Expression of shgScer\UAS.cSa in outer border cells, driven by Scer\GAL4slbo.2.6, delays border cell migration and blocks their detachment from the anterior follicular epithelium.
In shgScer\UAS.cSa; Scer\GAL4nos.UTR.T:Hsim\VP16 embryos, both the cell bodies and ensheathing cytoplasmic extensions of the somatic germ cell precursors are excluded from the cluster of germ cells.
When shgScer\UAS.cSa is driven by Scer\GAL4btl.PS tracheal development appears unaffected.
Cells of the optic placode maintain their epithelial shape but do not invaginate in embryos expressing shgScer\UAS.cSa under the control of Scer\GAL4da.G32. The Bolwig's organ does not separate from the optic lobe, in contrast to wild type.
Expression driven by Scer\GAL4en-e16E causes notching in the posterior compartment of the wing.
Clones in the wing disc expressing shgScer\UAS.cSa under the control of Scer\GAL4Act5C.PP form smooth borders with their neighbouring cells in both the anterior and posterior compartment. If in contact with each other, clones tend to fuse and interdigitate irrespective of the compartment boundary.
When expression is driven in clones in the embryonic epidermis by Scer\GAL4αTub84B.PP, the response of the cells to wg is blocked and ectopic denticles result. When the clones fall within the normal denticle belt, normal denticles are formed.
When expression is driven by Scer\GAL4en-e16E gaps in the wing margin occur.
Scer\GAL4arm.T:Hsim\VP16 mediated expression causes a cuticle phenotype identical to wgl-17. Scer\GAL4en-e16E mediated expression causes loss of wing margin in the posterior compartment.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
Statement
Reference
Scer\GAL4en-e16E, shgUAS.cSa has wing phenotype, suppressible by nej[+]/nej3
Suppressor of
Statement
Reference
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Expression of shgScer\UAS.cSa under the control of Scer\GAL4332.3 largely suppresses the embryonic lethality and dorsal closure defects in embryos expressing crbY10A in a crbGX24w-/crb11A22 background, resulting in some adult survival.
Expression of shgScer\UAS.cSa under the control of either Scer\GAL4upd1.PU or Scer\GAL4tj.PU fully rescues the decreased cell numbers of hub cells and cyst stem cells, and partially rescues the decreased number of germline stem cells, in the adult testicular niche of sdaiso7.8/Df(3R)ED6235 mutants. Expression of shgScer\UAS.cSa under the control of Scer\GAL4upd1.PU fails to rescue the decrease in germline stem cells with disintegrating fusomes seen in sdaiso7.8/Df(3R)ED6235 mutants. Expression of shgScer\UAS.cSa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 fails to rescue the germline stem cell, cyst stem cell, and hub cell number phenotypes in testicular niches of sdaiso7.8/Df(3R)ED6235 mutants.
The co-expression of both biScer\UAS.cBa and shgScer\UAS.cSa under the control of Scer\GAL4dpp.PU does not significantly suppress the long distance cell migration and scattering observed in third instar larval wing discs upon the expression of only biScer\UAS.cBa or only shgScer\UAS.cSa, under the control of Scer\GAL4dpp.PU.
Scer\GAL4unspecified-driven expression of shgScer\UAS.cSa suppresses the loss of follicle stem cells in somatic clones of CycEWX.
The extra secondary and tertiary pigment cell phenotype seen in the developing retinas of CskIR.Scer\UAS; Scer\GAL4GMR.PF animals at 42 hours after puparium formation is suppressed by shgScer\UAS.cSa.
The inhibition of border cell migration seen in the ovaries of jara.Scer\UAS; Scer\GAL4slbo.2.6 flies is significantly suppressed by the presence of shghs.PW under heat shock condition (flies raised at 29oC and given a 1 hour heat shock at 37oC 6-10 hours prior to dissection.). Partial suppression is also seen in flies carrying slboScer\UAS.cLa or shgScer\UAS.cSa, but not Fak56DScer\UAS.T:Ivir\HA1.
The wing phenotype caused by shgScer\UAS.cSa expressed under the control of Scer\GAL4en-e16E is exacerbated by heterozygosity for arm2 and suppressed by heterozygosity for nej3.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Expression of shgScer\UAS.cSa under the control of Scer\GAL4Scer\FRT.Rnor\CD2.αTub84B suppresses the follicle cell sorting, loss of epithelial integrity and oocyte mis-positioning phenotypes seen in shgR69 mutant follicle cell clones. Expression of shgScer\UAS.cSa can suppress the shgk03401 mutant follicle cell clone phenotype (follicle cell sorting, loss of epithelial integrity, and oocyte positioning). Expression of shgScer\UAS.cSa can suppress the migration defects of shgk03401 mutant border cell clones.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
shgScer\UAS.cSa
shgUAS.cSa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Sanson
Secondary FlyBase IDs
    References (29)