Allele Dmel\shg^Scer\UAS.cSa

General Information
Symbol	Dmel\shg^Scer\UAS.cSa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Sanson	FlyBase ID	FBal0056179
Feature type	allele	Associated gene	Dmel\shg

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Sanson et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: EcoRI fragment carrying full length shg cDNA is inserted into pUAST vector. (Sanson et al., 1996)
Carried in construct	P{UAS-shg.CAD} (Grzeschik and Knust, 2005, Barker et al., 2001, Schober et al., 2005, Dumstrei et al., 2002, Lee et al., 2003, Geisbrecht and Montell, 2002, Wang et al., 2004, Warner and Longmore, 2009, Jaiswal et al., 2006, Dahmann and Basler, 2000, Bao and Cagan, 2005, Collins and Treisman, 2000, Sanson et al., 1999, Waltzer and Bienz, 1998, Sanson et al., 1996, Jenkins et al., 2003, Almeida and Bray, 2005, Mirkovic and Mlodzik, 2006, Prakash et al., 2005, Vidal et al., 2006, Becam et al., 2005, Wang and Kalderon, 2009, Pereira et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	cell shape defective \| somatic clone (Jaiswal et al., 2006) lethal \| embryonic stage, with Scer\GAL4^{arm.T:Hsim\VP16} (Sanson et al., 1996)
Phenotype Manifest In
	Bolwig organ, with Scer\GAL4^da.G32 (Dumstrei et al., 2002) border follicle cell, with Scer\GAL4^slbo.2.6 (Schober et al., 2005) denticle & embryonic epidermis \| somatic clone, with Scer\GAL4^αTub84B.PP (Sanson et al., 1999) denticle \| ectopic \| somatic clone, with Scer\GAL4^αTub84B.PP (Sanson et al., 1999) denticle \| somatic clone, with Scer\GAL4^αTub84B.PP (Sanson et al., 1999) embryonic/first instar larval cuticle, with Scer\GAL4^{arm.T:Hsim\VP16} (Sanson et al., 1996) embryonic epidermis \| somatic clone, with Scer\GAL4^αTub84B.PP (Sanson et al., 1999) embryonic optic lobe, with Scer\GAL4^da.G32 (Dumstrei et al., 2002) embryonic optic lobe primordium, with Scer\GAL4^da.G32 (Dumstrei et al., 2002) fat body/gonad primordium, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Jenkins et al., 2003) gonadal sheath proper primordium, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Jenkins et al., 2003) ommatidium, with Scer\GAL4^hs.2sev (Mirkovic and Mlodzik, 2006) oocyte \| cell non-autonomous \| somatic clone, with Scer\GAL4^Act5C.PP (Becam et al., 2005) wing, with Scer\GAL4^en-e16E (Waltzer and Bienz, 1998) wing \| posterior compartment, with Scer\GAL4^en-e16E (Barker et al., 2001, Sanson et al., 1996) wing disc \| somatic clone, with Scer\GAL4^Act5C.PP (Dahmann and Basler, 2000) wing margin, with Scer\GAL4^en-e16E (Barker et al., 2001)
Detailed Description
	Statement Reference Expression of shg[Scer\UAS.cSa], under the control of Scer\GAL4[e22c], results in clones that are apically constricted. Somatic clones overexpressing shg[Scer\UAS.cSa] under the control of Scer\GAL4[vg.PM], separate from their neighbours and display smooth clone borders. (Jaiswal et al., 2006) Expression of shg^Scer\UAS.cSa under the control of Scer\GAL4^hs.2sev in ommatidia at the time of rotation has no effect on eyes when expressed at moderate levels and causes epithelial defects at high levels. The ommatidial under-rotation of Scer\GAL4^hs.2sev>shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} eyes is suppressed in Scer\GAL4^hs.2sev>shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs}; Scer\GAL4^hs.2sev>shg^Scer\UAS.cSa flies. Expression of shg^Scer\UAS.cSa under the control of Scer\GAL4^hs.2sev increases the number of ommatidial clusters that initiate rotation correctly in shg^k03401 clones. (Mirkovic and Mlodzik, 2006) Clones of follicle cells that are expressing shg^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PP can cause mislocalisation of the oocyte, and in these cases the mislocalised oocyte is always in contact with a cell or group of cells expressing shg^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PP. (Becam et al., 2005) Expression of shg^Scer\UAS.cSa, under the control of Scer\GAL4^elav-C155, does not suppress the R cell axon extension phenotype of Df(2L)CadN^Δ14 clones. (Prakash et al., 2005) Expression of shg^Scer\UAS.cSa in outer border cells, driven by Scer\GAL4^slbo.2.6, delays border cell migration and blocks their detachment from the anterior follicular epithelium. (Schober et al., 2005) In shg^Scer\UAS.cSa; Scer\GAL4^{nos.UTR.T:Hsim\VP16} embryos, both the cell bodies and ensheathing cytoplasmic extensions of the somatic germ cell precursors are excluded from the cluster of germ cells. (Jenkins et al., 2003) When shg^Scer\UAS.cSa is driven by Scer\GAL4^btl.PS tracheal development appears unaffected. (Lee et al., 2003) Cells of the optic placode maintain their epithelial shape but do not invaginate in embryos expressing shg^Scer\UAS.cSa under the control of Scer\GAL4^da.G32. The Bolwig's organ does not separate from the optic lobe, in contrast to wild type. (Dumstrei et al., 2002) Expression driven by Scer\GAL4^en-e16E causes notching in the posterior compartment of the wing. (Barker et al., 2001) Clones in the wing disc expressing shg^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PP form smooth borders with their neighbouring cells in both the anterior and posterior compartment. If in contact with each other, clones tend to fuse and interdigitate irrespective of the compartment boundary. (Dahmann and Basler, 2000) When expression is driven in clones in the embryonic epidermis by Scer\GAL4^αTub84B.PP, the response of the cells to wg is blocked and ectopic denticles result. When the clones fall within the normal denticle belt, normal denticles are formed. (Sanson et al., 1999) When expression is driven by Scer\GAL4^en-e16E gaps in the wing margin occur. (Waltzer and Bienz, 1998) Scer\GAL4^{arm.T:Hsim\VP16} mediated expression causes a cuticle phenotype identical to wg^l-17. Scer\GAL4^en-e16E mediated expression causes loss of wing margin in the posterior compartment. (Sanson et al., 1996)
External Data
Linkouts
Interactions
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Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^en-e16E, shg^Scer\UAS.cSa has wing \| posterior compartment phenotype, enhanceable by brm[+]/brm² (Barker et al., 2001) Scer\GAL4^en-e16E, shg^Scer\UAS.cSa has wing \| posterior compartment phenotype, enhanceable by mor²/mor[+] (Barker et al., 2001) Scer\GAL4^en-e16E, shg^Scer\UAS.cSa has wing \| posterior compartment phenotype, enhanceable by pan[+]/pan² (Barker et al., 2001) Scer\GAL4^en-e16E, shg^Scer\UAS.cSa has wing margin phenotype, enhanceable by brm[+]/brm² (Barker et al., 2001) Scer\GAL4^en-e16E, shg^Scer\UAS.cSa has wing margin phenotype, enhanceable by mor²/mor[+] (Barker et al., 2001) Scer\GAL4^en-e16E, shg^Scer\UAS.cSa has wing margin phenotype, enhanceable by pan[+]/pan² (Barker et al., 2001) Scer\GAL4^en-e16E, shg^Scer\UAS.cSa has wing phenotype, enhanceable by arm²/arm[+] (Waltzer and Bienz, 1998)
Suppressed by
Statement Reference Scer\GAL4^en-e16E, shg^Scer\UAS.cSa has wing phenotype, suppressible by nej[+]/nej³ (Waltzer and Bienz, 1998)
Suppressor of
Statement Reference shg^Scer\UAS.cSa/Scer\GAL4^slbo.2.6 is a suppressor \| partially of border follicle cell phenotype of Scer\GAL4^slbo.2.6, jar^a.Scer\UAS (Geisbrecht and Montell, 2002) shg^Scer\UAS.cSa/Scer\GAL4^unspecified is a suppressor of follicle stem cell \| somatic clone phenotype of CycE^WX (Wang and Kalderon, 2009) shg^Scer\UAS.cSa is a suppressor of secondary pigment cell \| supernumerary phenotype of Csk^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Vidal et al., 2006) shg^Scer\UAS.cSa is a suppressor of tertiary pigment cell \| supernumerary phenotype of Csk^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Vidal et al., 2006)
NOT Suppressor of
Statement Reference shg^Scer\UAS.cSa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI is a non-suppressor of adherens junction \| somatic clone phenotype of Rho1^{dsRNA.1.Scer\UAS}, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI (Warner and Longmore, 2009)
Additional Comments
Genetic Interactions
Statement Reference Scer\GAL4[unspecified]-driven expression of shg[Scer\UAS.cSa] suppresses the loss of follicle stem cells in somatic clones of CycE[WX]. (Wang and Kalderon, 2009) The extra secondary and tertiary pigment cell phenotype seen in the developing retinas of Csk[IR.Scer\UAS]; Scer\GAL4[GMR.PF] animals at 42 hours after puparium formation is suppressed by shg[Scer\UAS.cSa]. (Vidal et al., 2006) The inhibition of border cell migration seen in the ovaries of jar^a.Scer\UAS; Scer\GAL4^slbo.2.6 flies is significantly suppressed by the presence of shg^hs.PW under heat shock condition (flies raised at 29^oC and given a 1 hour heat shock at 37^oC 6-10 hours prior to dissection.). Partial suppression is also seen in flies carrying slbo^Scer\UAS.cLa or shg^Scer\UAS.cSa, but not Fak56D^{Scer\UAS.T:Ivir\HA1}. (Geisbrecht and Montell, 2002) The wing phenotype caused by shg^Scer\UAS.cSa expressed under the control of Scer\GAL4^en-e16E is exacerbated by heterozygosity for arm² and suppressed by heterozygosity for nej³. (Waltzer and Bienz, 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	shg^Scer\UAS.cSa/Scer\GAL4^arm.PS rescues shg¹ (Sanson et al., 1996) shg^Scer\UAS.cSa/Scer\GAL4^arm.PS rescues shg² (Sanson et al., 1996)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	shg^Scer\UAS.cSa shg^UAS.cSa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Sanson (Sanson et al., 1996)
Secondary FlyBase IDs

References ( 23 )

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List References by type	Research paper ( 23 )
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Allele Dmel\shgScer\UAS.cSa

Allele Dmel\shg^Scer\UAS.cSa