Overexpression of shgScer\UAS.cSa
6-fold in nurse cells (under the control of Scer\GAL4otu.T:Hsim\VP16
, and Scer\GAL4nos.UTR.T:Hsim\VP16
) results in border cells migrating along a normal trajectory more slowly than normal and exhibiting a hyperpolarized morphology. A total of 86% of protrusions from border cell clusters migrating between nurse cells are directed toward the oocyte (compared to 76% in wild-type).
Overexpression of shg
in outer, migratory border cells using Scer\GAL4slbo.2.6
or in polar cell using Scer\GAL4upd.PU
does not affect border cell migration significantly.
The expression of shgScer\UAS.cSa
under the control of Scer\GAL4dpp.PU
induces long distance cell migration and scattering, even across the anterior-posterior compartment border, in third instar larval wing discs, as compared to controls.
Expression of shgScer\UAS.cSa
in outer border cells, driven by Scer\GAL4slbo.2.6
, delays border cell migration and blocks their detachment from the anterior follicular epithelium.
Cells of the optic placode maintain their epithelial shape but do not invaginate in embryos expressing shgScer\UAS.cSa
under the control of Scer\GAL4da.G32
. The Bolwig's organ does not separate from the optic lobe, in contrast to wild type.
Expression driven by Scer\GAL4en-e16E
causes notching in the posterior compartment of the wing.
Clones in the wing disc expressing shgScer\UAS.cSa
under the control of Scer\GAL4Act5C.PP
form smooth borders with their neighbouring cells in both the anterior and posterior compartment. If in contact with each other, clones tend to fuse and interdigitate irrespective of the compartment boundary.
When expression is driven in clones in the embryonic epidermis by Scer\GAL4αTub84B.PP
, the response of the cells to wg
is blocked and ectopic denticles result. When the clones fall within the normal denticle belt, normal denticles are formed.