|Name||Saccharomyces cerevisiae UAS construct a of Wang||FlyBase ID||FBal0056360|
|Feature type||allele||Associated gene||Dmel\numb|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
(Chien et al., 1998, Lundell et al., 2003, Wan et al., 2000, Younger, 2001.2, Edenfeld et al., 2007, Ainsworth et al., 2000, Nagel et al., 2000, Reddy and Rodrigues, 1999, Griffiths and Hidalgo, 2004, Wang et al., 1997, Zhong et al., 1997, Merdes et al., 2004, Sen et al., 2003, Zhong et al., 1996, Frise et al., 1996, Carmena et al., 1998, Lee et al., 2006, Griffiths et al., 2007, Rebeiz et al., 2011, Xie et al., 2010)
|Phenotype Manifest In|
Expression of numb[Scer\UAS.cWa] in the wing disc under the control of Scer\GAL4[ap-md544] inhibits the production of hairs during asymmetric division of the sensory organ precursors, which results in bald nota in all progeny.
Expression of numbScer\UAS.cWa in peripheral glial cells under the control of Scer\GAL4Mz97 leads to an impairment of glial cell migration. The ventral peripheral glial cells and the ventral intersegmental peripheral glial cells stall at the exit zone, while the medial intersegmental peripheral glial cells migrate further laterally. Ventral peripheral glia in which numbScer\UAS.cWa is not expressed due to the pattern of the Scer\GAL4Mz97 driver migrate dorsally.
When numbScer\UAS.cWa is driven by Scer\GAL4sca-537.4, bristle formation is inhibited leading to bald patches of cuticle, and the flies die shortly after hatching.
When numbScer\UAS.cWa is driven by Scer\GAL4sca-P309, a strong reduction in external structures on the adult antenna is seen, with a concomitant increase in glial cell numbers, with a concomitant increase in glial cell numbers. The diameter of fascicles in antennal segment 3 are also increased.
Lethality, when driven by Scer\GAL4109-53 or Scer\GAL4109-79 acts in the embryonic-larval stages. When driven by Scer\GAL4sca-109-68 bristle defects occur.
Expression of numbScer\UAS.cWa under the control of Scer\GAL4unspecified in the Malpighian tubules results in two tip cells developing, and the tubules are not arranged normally.
When numbScer\UAS.cWa is driven by Scer\GAL4hs.PB from 3.5 to 7.5 hours after egg laying, Malpighian tubules have two tip cells, instead of one as seen in wild-type. However, there is no change in the final number of tubule cells. The distinction between the number of cells in the anterior and posterior tubules is also unaffected.
The number of eve-expressing pericardial cells is reduced and the number of DA1 muscles is increased in embryos expressing numbScer\UAS.cWa under the control of Scer\GAL4twi.PG.
Embryos expressing numbScer\UAS.cWa under the control of Scer\GAL4sca-537.4 show transformation of sheath cells to neurons in the chordotonal organs. Overexpression of numbScer\UAS.cWa under the control of Scer\GAL4sca-109-68 in adult sensory organs produces a balding phenotype (external sensory organs have no hair and no socket).
Embryos expressing numbScer\UAS.cWa under the control of Scer\GAL4twi.PB show duplications and transformations of muscles, and adult muscle precursors are lost. Muscle VA2 is duplicated at the expense of VA1, DO5 is transformed to LL1 and LT3 is transformed to LT4. The ventral adult muscle precursors are missing and muscle VA3 is duplicated. Lateral and dorsal adult muscle precursors are also missing, and the segment border muscle and dorsal muscles (including DA1) are duplicated. Embryos lack pericardial cells. Despite the muscle aberrations, some embryos develop to form adult flies. These flies are flightless, do not jump and lack ventral abdominal muscles that would normally be generated from the ventral adult muscle precursors.
Scer\GAL4sca-109-68-mediated expression causes twinned hairs and balding. Phenotype is caused by transformation of both the IIa and IIb cell and the sheath to a neuron.
When expression is driven by Scer\GAL4ptc-559.1, at 29oC during the second larval instar stage, adult flies show a loss of distal wing margin at the anterior/posterior boundary. When expressed in the sensory organ precursor cells of the adult external sense organs, using Scer\GAL4sca-109-68, a twinned hair phenotype is produced.
|Phenotype Manifest In|
|NOT Enhanced by|
Scer\GAL4ap-md544, numbScer\UAS.cWa has mesothoracic tergum phenotype, non-enhanceable by Scer\GAL4ap-md544, numbScer\UAS.cWa
Scer\GAL4sca-537.4, numbScer\UAS.cWa has macrochaeta phenotype, suppressible by Hsap\APLP2::Hsap\APPScer\UAS.T:Hsap\MYC, Scer\GAL4sca-537.4
|NOT Enhancer of|
Scer\GAL4ap-md544, numbScer\UAS.cWa is a non-enhancer of mesothoracic tergum phenotype of Scer\GAL4ap-md544, numbScer\UAS.cWa
numbScer\UAS.cWa, Scer\GAL4sca-537.4 is a suppressor of mechanosensory sensory organ phenotype of Hsap\APLP2::Hsap\APPScer\UAS.T:Hsap\MYC, Scer\GAL4sca-537.4
|NOT Suppressor of|
Expression of mol[Scer\UAS.cXa] in the wing disc does not enhance the bald notum phenotype seen upon expression of numb[Scer\UAS.cWa] under the control of Scer\GAL4[ap-md544].
Co-expression of numbScer\UAS.cWa with Su(H)Scer\UAS.cNa driven by Scer\GAL4sca-537.4 results in flies that are partially bald.
The addition of numbScer\UAS.cWa driven by Scer\GAL4sca-537.4 to H99/HP8 flies rescues the double socket phenotype seen in the external sensory organs in these flies.
The balding phenotype produced by expression of numbScer\UAS.cWa under the control of Scer\GAL4sca-109-68 is enhanced by Df(2L)TW3. The cell fate transformation phenotypes produced by overexpression of either numbScer\UAS.cWa or NakScer\UAS.cCa in the adult sensory organs are suppressed in flies expressing both numbScer\UAS.cWa and NakScer\UAS.cCa in the adult sensory organs under the control of Scer\GAL4sca-109-68.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of Wang
|Secondary FlyBase IDs|
|References ( 21 )|
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|List References by type|
|Recent research papers ( 1 )|