Allele Dmel\numbScer\UAS.cWa
| General Information | |||
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| Symbol | Dmel\numbScer\UAS.cWa | Species | D. melanogaster |
| Name | Saccharomyces cerevisiae UAS construct a of Wang | FlyBase ID | FBal0056360 |
| Feature type | allele | Associated gene | Dmel\numb |
| Allele class | |||
| Mutagen | in vitro construct - regulatory fusion | ||
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| Description |
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| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
Nature of the Allele
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| Allele class | |||
| Mutagen | |||
| Mutations Mapped to the Genome | |||
Type Location Additional Notes References | |||
| Associated Sequence Data | |||
| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | ||
| UniProtKB/Swiss-Prot | |||
| UniProtKB/TrEMBL | |||
| Progenitor genotype | |||
| Nature of the lesion | Statement Reference | ||
| Carried in construct | (Chien et al., 1998, Lundell et al., 2003, Wan et al., 2000, Younger, 2001.2, Edenfeld et al., 2007, Ainsworth et al., 2000, Nagel et al., 2000, Reddy and Rodrigues, 1999, Griffiths and Hidalgo, 2004, Wang et al., 1997, Zhong et al., 1997, Merdes et al., 2004, Sen et al., 2003, Zhong et al., 1996, Frise et al., 1996, Carmena et al., 1998, Lee et al., 2006, Griffiths et al., 2007, Rebeiz et al., 2011, Xie et al., 2010) | ||
| Cytology | |||
Phenotypic Data
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Phenotypic Class
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lethal, with Scer\GAL4109-53 lethal, with Scer\GAL4109-79 | |||
Phenotype Manifest In
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fascicle & antennal segment 3, with Scer\GAL4sca-P309 glial cell & antennal segment 3 | ectopic, with Scer\GAL4sca-P309 microchaeta & antennal segment 3, with Scer\GAL4sca-P309 | |||
Detailed Description
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Statement Reference Expression of numb[Scer\UAS.cWa] in the wing disc under the control of Scer\GAL4[ap-md544] inhibits the production of hairs during asymmetric division of the sensory organ precursors, which results in bald nota in all progeny. Expression of numbScer\UAS.cWa in peripheral glial cells under the control of Scer\GAL4Mz97 leads to an impairment of glial cell migration. The ventral peripheral glial cells and the ventral intersegmental peripheral glial cells stall at the exit zone, while the medial intersegmental peripheral glial cells migrate further laterally. Ventral peripheral glia in which numbScer\UAS.cWa is not expressed due to the pattern of the Scer\GAL4Mz97 driver migrate dorsally. When numbScer\UAS.cWa is driven by Scer\GAL4sca-537.4, bristle formation is inhibited leading to bald patches of cuticle, and the flies die shortly after hatching. When numbScer\UAS.cWa is driven by Scer\GAL4sca-P309, a strong reduction in external structures on the adult antenna is seen, with a concomitant increase in glial cell numbers, with a concomitant increase in glial cell numbers. The diameter of fascicles in antennal segment 3 are also increased. Lethality, when driven by Scer\GAL4109-53 or Scer\GAL4109-79 acts in the embryonic-larval stages. When driven by Scer\GAL4sca-109-68 bristle defects occur. Expression of numbScer\UAS.cWa under the control of Scer\GAL4unspecified in the Malpighian tubules results in two tip cells developing, and the tubules are not arranged normally. When numbScer\UAS.cWa is driven by Scer\GAL4hs.PB from 3.5 to 7.5 hours after egg laying, Malpighian tubules have two tip cells, instead of one as seen in wild-type. However, there is no change in the final number of tubule cells. The distinction between the number of cells in the anterior and posterior tubules is also unaffected. The number of eve-expressing pericardial cells is reduced and the number of DA1 muscles is increased in embryos expressing numbScer\UAS.cWa under the control of Scer\GAL4twi.PG. Embryos expressing numbScer\UAS.cWa under the control of Scer\GAL4sca-537.4 show transformation of sheath cells to neurons in the chordotonal organs. Overexpression of numbScer\UAS.cWa under the control of Scer\GAL4sca-109-68 in adult sensory organs produces a balding phenotype (external sensory organs have no hair and no socket). Embryos expressing numbScer\UAS.cWa under the control of Scer\GAL4twi.PB show duplications and transformations of muscles, and adult muscle precursors are lost. Muscle VA2 is duplicated at the expense of VA1, DO5 is transformed to LL1 and LT3 is transformed to LT4. The ventral adult muscle precursors are missing and muscle VA3 is duplicated. Lateral and dorsal adult muscle precursors are also missing, and the segment border muscle and dorsal muscles (including DA1) are duplicated. Embryos lack pericardial cells. Despite the muscle aberrations, some embryos develop to form adult flies. These flies are flightless, do not jump and lack ventral abdominal muscles that would normally be generated from the ventral adult muscle precursors. Scer\GAL4sca-109-68-mediated expression causes twinned hairs and balding. Phenotype is caused by transformation of both the IIa and IIb cell and the sheath to a neuron. When expression is driven by Scer\GAL4ptc-559.1, at 29oC during the second larval instar stage, adult flies show a loss of distal wing margin at the anterior/posterior boundary. When expressed in the sensory organ precursor cells of the adult external sense organs, using Scer\GAL4sca-109-68, a twinned hair phenotype is produced. | |||
External Data
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| Linkouts | |||
Interactions
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Phenotypic Class
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Enhanced by | |||
Statement Reference | |||
Suppressed by | |||
Statement Reference Scer\GAL4sca-537.4, numbScer\UAS.cWa has lethal | adult stage phenotype, suppressible by Hsap\APLP2::Hsap\APPScer\UAS.T:Hsap\MYC, Scer\GAL4sca-537.4 | |||
Suppressor of | |||
Statement Reference Scer\GAL4wor.PA/numbScer\UAS.cWa is a suppressor | partially of neuroanatomy defective phenotype of aur14641 | |||
Other | |||
Statement Reference | |||
Phenotype Manifest In
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Enhanced by | |||
Statement Reference | |||
NOT Enhanced by | |||
Statement Reference Scer\GAL4ap-md544, numbScer\UAS.cWa has mesothoracic tergum phenotype, non-enhanceable by Scer\GAL4ap-md544, numbScer\UAS.cWa Scer\GAL4ap-md544, numbScer\UAS.cWa has notal microchaeta phenotype, non-enhanceable by Scer\GAL4ap-md544, numbScer\UAS.cWa | |||
Suppressed by | |||
Statement Reference Scer\GAL4sca-537.4, numbScer\UAS.cWa has macrochaeta phenotype, suppressible by Hsap\APLP2::Hsap\APPScer\UAS.T:Hsap\MYC, Scer\GAL4sca-537.4 Scer\GAL4sca-537.4, numbScer\UAS.cWa has mechanosensory sensory organ phenotype, suppressible by Hsap\APLP2::Hsap\APPScer\UAS.T:Hsap\MYC, Scer\GAL4sca-537.4 | |||
Enhancer of | |||
Statement Reference | |||
NOT Enhancer of | |||
Statement Reference Scer\GAL4ap-md544, numbScer\UAS.cWa is a non-enhancer of mesothoracic tergum phenotype of Scer\GAL4ap-md544, numbScer\UAS.cWa Scer\GAL4ap-md544, numbScer\UAS.cWa is a non-enhancer of notal microchaeta phenotype of Scer\GAL4ap-md544, numbScer\UAS.cWa | |||
Suppressor of | |||
Statement Reference numbScer\UAS.cWa, Scer\GAL4sca-537.4 is a suppressor of mechanosensory sensory organ phenotype of Hsap\APLP2::Hsap\APPScer\UAS.T:Hsap\MYC, Scer\GAL4sca-537.4 Scer\GAL4wor.PA/numbScer\UAS.cWa is a suppressor | partially of neuroblast | supernumerary & larval brain phenotype of aur14641 | |||
NOT Suppressor of | |||
Statement Reference Scer\GAL4wor.PA/numbScer\UAS.cWa is a non-suppressor of spindle & neuroblast & larva phenotype of aur8839 | |||
Other | |||
Statement Reference | |||
Additional Comments
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Genetic Interactions
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Statement Reference Expression of mol[Scer\UAS.cXa] in the wing disc does not enhance the bald notum phenotype seen upon expression of numb[Scer\UAS.cWa] under the control of Scer\GAL4[ap-md544]. Co-expression of numbScer\UAS.cWa with Su(H)Scer\UAS.cNa driven by Scer\GAL4sca-537.4 results in flies that are partially bald. The addition of numbScer\UAS.cWa driven by Scer\GAL4sca-537.4 to H99/HP8 flies rescues the double socket phenotype seen in the external sensory organs in these flies. The balding phenotype produced by expression of numbScer\UAS.cWa under the control of Scer\GAL4sca-109-68 is enhanced by Df(2L)TW3. The cell fate transformation phenotypes produced by overexpression of either numbScer\UAS.cWa or NakScer\UAS.cCa in the adult sensory organs are suppressed in flies expressing both numbScer\UAS.cWa and NakScer\UAS.cCa in the adult sensory organs under the control of Scer\GAL4sca-109-68. When expression is driven by Scer\GAL4ptc-559.1 (without increasing the temperature to 29oC) in N55e11/+ flies, an even stronger notched wing phenotype is seen. | |||
Xenogenetic Interactions
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Statement Reference | |||
Complementation & Rescue Data
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| Comments | |||
Stocks
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Notes on Origin
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| Discoverer | S. Wang. | ||
External Crossreferences & Linkouts
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Synonyms & Secondary IDs
( 3 ) | |||
| Reported As | |||
| Symbol Synonym | numbScer\UAS.cWa numbUAS.cWa | ||
| Name Synonym | Saccharomyces cerevisiae UAS construct a of Wang | ||
| Secondary FlyBase IDs | |||
References
( 21 ) | |||
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Recent research papers ( 1 ) | |||
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External Crossreferences & Linkouts