FB2025_01 , released February 20, 2025
Allele: Dmel\numbUAS.cWa
Open Close
General Information
Symbol
Dmel\numbUAS.cWa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Wang
FlyBase ID
FBal0056360
Feature type
allele
Associated gene
Dmel\numb
Associated Insertion(s)
Carried in Construct
P{UAS-numb.W}
Also Known As
UAS-numb, UAS-Nb, UASnumb
Key Links
Transgenic product class
wild_type
(Chien et al., 1998)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Chien et al., 1998)
Mutagen
in vitro construct
(Chien et al., 1998, Frise et al., 1996, Zhong et al., 1996)
Progenitor genotype
Carried in construct
P{UAS-numb.W}
Cytology
Description

Full length numb is expressed under the control of UASt regulatory sequences.

(Chien et al., 1998)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
numb
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal 1 dorsal adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 1 lateral adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 1 segment border muscle cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 1 ventral adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 2 dorsal adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 2 lateral adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 2 segment border muscle cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 2 ventral adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 3 dorsal adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 3 lateral adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 3 segment border muscle cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 3 ventral adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 4 dorsal adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 4 lateral adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 4 segment border muscle cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 4 ventral adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 5 dorsal adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 5 lateral adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 5 segment border muscle cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 5 ventral adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 6 dorsal adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 6 lateral adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 6 segment border muscle cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 6 ventral adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 7 dorsal adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 7 lateral adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 7 segment border muscle cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal 7 ventral adult muscle precursor cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      abdominal ventral muscle cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      adult sense organ, with Scer\GAL4sca-109-68

      (Chien et al., 1998)

      antenna, with Scer\GAL4sca-P309

      (Sen et al., 2003)

      dorsal acute muscle cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      dorsal oblique muscle cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      dorsal transverse muscle cell, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      embryonic Malpighian tubule, with Scer\GAL4unspecified

      (Ainsworth et al., 2000)

      embryonic Malpighian tubule tip cell | ectopic, with Scer\GAL4unspecified

      (Ainsworth et al., 2000)

      external sensory organ, with Scer\GAL4sca-109-68

      (Frise et al., 1996)

      external sensory organ precursor cell IIa, with Scer\GAL4sca-109-68

      (Wang et al., 1997)

      external sensory organ precursor cell IIb, with Scer\GAL4sca-109-68

      (Wang et al., 1997)

      fascicle & antennal segment 3, with Scer\GAL4sca-P309

      (Sen et al., 2003)

      ganglion mother cell | somatic clone | third instar larval stage

      (Toriya et al., 2006)

      glial cell & antennal segment 3 | ectopic, with Scer\GAL4sca-P309

      (Sen et al., 2003)

      hypodermal muscle cell of larval abdominal 9, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      larval neuroblast | somatic clone | third instar larval stage

      (Toriya et al., 2006)

      larval outer optic anlage | somatic clone | third instar larval stage

      (Toriya et al., 2006)

      macrochaeta, with Scer\GAL4sca-109-68

      (Younger, 2001.2)

      macrochaeta, with Scer\GAL4sca-537.4

      (Merdes et al., 2004)

      Malpighian tubule tip cell, with Scer\GAL4hs.PB

      (Wan et al., 2000)

      mechanosensory sensory organ, with Scer\GAL4sca-537.4

      (Merdes et al., 2004)

      mesonotum, with Scer\GAL4ap-md544

      (Xie et al., 2010)

      microchaeta & antennal segment 3, with Scer\GAL4sca-P309

      (Sen et al., 2003)

      muscle cell of abdominal 1 dorsal acute muscle 1, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 1 dorsal acute muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of abdominal 1 dorsal oblique muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of abdominal 1 dorsal oblique muscle 5, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 1 lateral transverse muscle 3, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 1 ventral acute muscle 1, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 2 dorsal acute muscle 1, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 2 dorsal acute muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of abdominal 2 dorsal oblique muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of abdominal 2 dorsal oblique muscle 5, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 2 lateral transverse muscle 3, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 2 ventral acute muscle 1, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 3 dorsal acute muscle 1, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 3 dorsal acute muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of abdominal 3 dorsal oblique muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of abdominal 3 dorsal oblique muscle 5, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 3 lateral transverse muscle 3, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 3 ventral acute muscle 1, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 4 dorsal acute muscle 1, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 4 dorsal acute muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of abdominal 4 dorsal oblique muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of abdominal 4 dorsal oblique muscle 5, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 4 lateral transverse muscle 3, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 4 ventral acute muscle 1, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 5 dorsal acute muscle 1, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 5 dorsal acute muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of abdominal 5 dorsal oblique muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of abdominal 5 dorsal oblique muscle 5, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 5 lateral transverse muscle 3, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 5 ventral acute muscle 1, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 6 dorsal acute muscle 1, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 6 dorsal acute muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of abdominal 6 dorsal oblique muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of abdominal 6 dorsal oblique muscle 5, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 6 lateral transverse muscle 3, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 6 ventral acute muscle 1, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 7 dorsal acute muscle 1, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 7 dorsal acute muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of abdominal 7 dorsal oblique muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of abdominal 7 dorsal oblique muscle 5, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 7 lateral transverse muscle 3, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 7 ventral acute muscle 1, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      muscle cell of abdominal 8 dorsal oblique muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of mesothoracic dorsal acute muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of mesothoracic dorsal oblique muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of metathoracic dorsal acute muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of metathoracic dorsal oblique muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      muscle cell of prothoracic dorsal oblique muscle 1, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      neuron | somatic clone | third instar larval stage

      (Toriya et al., 2006)

      notal microchaeta, with Scer\GAL4ap-md544

      (Xie et al., 2010)

      pericardial cell | embryonic stage, with Scer\GAL4twi.PB

      (Ruiz Gomez and Bate, 1997)

      pericardial cell | embryonic stage, with Scer\GAL4twi.PG

      (Carmena et al., 1998)

      PNS glial cell, with Scer\GAL4Ntan1-Mz97

      (Edenfeld et al., 2007)

      scolopale cell | embryonic stage, with Scer\GAL4sca-537.4

      (Chien et al., 1998)

      tormogen cell, with Scer\GAL4sca-109-68

      (Wang et al., 1997)

      wing, with Scer\GAL4ptc-559.1

      (Frise et al., 1996)
      Detailed Description
      Statement
      Reference

      Expression of numbScer\UAS.cWa in the wing disc under the control of Scer\GAL4ap-md544 inhibits the production of hairs during asymmetric division of the sensory organ precursors, which results in bald nota in all progeny.

      (Xie et al., 2010)

      Expression of numbScer\UAS.cWa in peripheral glial cells under the control of Scer\GAL4Mz97 leads to an impairment of glial cell migration. The ventral peripheral glial cells and the ventral intersegmental peripheral glial cells stall at the exit zone, while the medial intersegmental peripheral glial cells migrate further laterally. Ventral peripheral glia in which numbScer\UAS.cWa is not expressed due to the pattern of the Scer\GAL4Mz97 driver migrate dorsally.

      (Edenfeld et al., 2007)

      Compared to control clones in third instar larvae, clones with expression of numbScer\UAS.cWa have a cell cycle delay: there are a reduced number of neuroepithelial stem cells that have not differentiated into neurons on the lateral surface of the brain, and post-embryonic neuroblasts contain smaller numbers of neurons and ganglion mother cells; Hsap\NUMBScer\UAS.PTBS-PRRS clones containing only neurons on the medial surface near the central brain are found at a higher frequency than control clones.

      (Toriya et al., 2006)

      When numbScer\UAS.cWa is driven by Scer\GAL4sca-537.4, bristle formation is inhibited leading to bald patches of cuticle, and the flies die shortly after hatching.

      (Merdes et al., 2004)

      When numbScer\UAS.cWa is driven by Scer\GAL4sca-P309, a strong reduction in external structures on the adult antenna is seen, with a concomitant increase in glial cell numbers, with a concomitant increase in glial cell numbers. The diameter of fascicles in antennal segment 3 are also increased.

      (Sen et al., 2003)

      Lethality, when driven by Scer\GAL4109-53 or Scer\GAL4109-79 acts in the embryonic-larval stages. When driven by Scer\GAL4sca-109-68 bristle defects occur.

      (Younger, 2001.2)

      Expression of numbScer\UAS.cWa under the control of Scer\GAL4unspecified in the Malpighian tubules results in two tip cells developing, and the tubules are not arranged normally.

      (Ainsworth et al., 2000)

      When numbScer\UAS.cWa is driven by Scer\GAL4hs.PB from 3.5 to 7.5 hours after egg laying, Malpighian tubules have two tip cells, instead of one as seen in wild-type. However, there is no change in the final number of tubule cells. The distinction between the number of cells in the anterior and posterior tubules is also unaffected.

      (Wan et al., 2000)

      The number of eve-expressing pericardial cells is reduced and the number of DA1 muscles is increased in embryos expressing numbScer\UAS.cWa under the control of Scer\GAL4twi.PG.

      (Carmena et al., 1998)

      Embryos expressing numbScer\UAS.cWa under the control of Scer\GAL4sca-537.4 show transformation of sheath cells to neurons in the chordotonal organs. Overexpression of numbScer\UAS.cWa under the control of Scer\GAL4sca-109-68 in adult sensory organs produces a balding phenotype (external sensory organs have no hair and no socket).

      (Chien et al., 1998)

      Embryos expressing numbScer\UAS.cWa under the control of Scer\GAL4twi.PB show duplications and transformations of muscles, and adult muscle precursors are lost. Muscle VA2 is duplicated at the expense of VA1, DO5 is transformed to LL1 and LT3 is transformed to LT4. The ventral adult muscle precursors are missing and muscle VA3 is duplicated. Lateral and dorsal adult muscle precursors are also missing, and the segment border muscle and dorsal muscles (including DA1) are duplicated. Embryos lack pericardial cells. Despite the muscle aberrations, some embryos develop to form adult flies. These flies are flightless, do not jump and lack ventral abdominal muscles that would normally be generated from the ventral adult muscle precursors.

      (Ruiz Gomez and Bate, 1997)

      Scer\GAL4sca-109-68-mediated expression causes twinned hairs and balding. Phenotype is caused by transformation of both the IIa and IIb cell and the sheath to a neuron.

      (Wang et al., 1997)

      When expression is driven by Scer\GAL4ptc-559.1, at 29oC during the second larval instar stage, adult flies show a loss of distal wing margin at the anterior/posterior boundary. When expressed in the sensory organ precursor cells of the adult external sense organs, using Scer\GAL4sca-109-68, a twinned hair phenotype is produced.

      (Frise et al., 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Scer\GAL4sca-109-68, numbUAS.cWa has visible phenotype, enhanceable by Df(2L)TW3

      (Chien et al., 1998)
      Suppressed by
      Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Scer\GAL4sca-109-68, numbUAS.cWa has adult sense organ phenotype, enhanceable by Df(2L)TW3

      (Chien et al., 1998)
      NOT Enhanced by
      Suppressed by
      Enhancer of
      Statement
      Reference

      Scer\GAL4ptc-559.1/numbUAS.cWa is an enhancer of wing phenotype of N55e11

      (Frise et al., 1996)
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4wor.PA/numbUAS.cWa is a non-suppressor of spindle & neuroblast & larva phenotype of aurA8839

      (Lee et al., 2006)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of molScer\UAS.cXa in the wing disc does not enhance the bald notum phenotype seen upon expression of numbScer\UAS.cWa under the control of Scer\GAL4ap-md544.

      (Xie et al., 2010)

      Co-expression of numbScer\UAS.cWa with Su(H)Scer\UAS.cNa driven by Scer\GAL4sca-537.4 results in flies that are partially bald.

      The addition of numbScer\UAS.cWa driven by Scer\GAL4sca-537.4 to H99/HP8 flies rescues the double socket phenotype seen in the external sensory organs in these flies.

      (Nagel et al., 2000)

      The balding phenotype produced by expression of numbScer\UAS.cWa under the control of Scer\GAL4sca-109-68 is enhanced by Df(2L)TW3. The cell fate transformation phenotypes produced by overexpression of either numbScer\UAS.cWa or NakScer\UAS.cCa in the adult sensory organs are suppressed in flies expressing both numbScer\UAS.cWa and NakScer\UAS.cCa in the adult sensory organs under the control of Scer\GAL4sca-109-68.

      (Chien et al., 1998)

      When expression is driven by Scer\GAL4ptc-559.1 (without increasing the temperature to 29oC) in N55e11/+ flies, an even stronger notched wing phenotype is seen.

      (Frise et al., 1996)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4Tub.PU/numbUAS.cWa rescues numb15

      (Toriya et al., 2006)
      Comments

      Expression of numbScer\UAS.cWa rescues neuronal differentiation defects seen in the outer optic anlage of numb15/numb15 clones in third instar larvae.

      (Toriya et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      S. Wang.

      (Frise et al., 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      numbScer\UAS.cWa
      numbUAS.cWa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Wang
      (Frise et al., 1996)
      Secondary FlyBase IDs
        References (28)