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General Information
Symbol
Dmel\numbUAS.cWa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Wang
FlyBase ID
FBal0056360
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-numb, UAS-Nb, UASnumb
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
Full length numb is expressed under the control of UASt regulatory sequences.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
fascicle & antennal segment 3, with Scer\GAL4sca-P309
glial cell & antennal segment 3 | ectopic, with Scer\GAL4sca-P309
microchaeta & antennal segment 3, with Scer\GAL4sca-P309
Detailed Description
Statement
Reference
Expression of numbScer\UAS.cWa in the wing disc under the control of Scer\GAL4ap-md544 inhibits the production of hairs during asymmetric division of the sensory organ precursors, which results in bald nota in all progeny.
Expression of numbScer\UAS.cWa in peripheral glial cells under the control of Scer\GAL4Mz97 leads to an impairment of glial cell migration. The ventral peripheral glial cells and the ventral intersegmental peripheral glial cells stall at the exit zone, while the medial intersegmental peripheral glial cells migrate further laterally. Ventral peripheral glia in which numbScer\UAS.cWa is not expressed due to the pattern of the Scer\GAL4Mz97 driver migrate dorsally.
Compared to control clones in third instar larvae, clones with expression of numbScer\UAS.cWa have a cell cycle delay: there are a reduced number of neuroepithelial stem cells that have not differentiated into neurons on the lateral surface of the brain, and post-embryonic neuroblasts contain smaller numbers of neurons and ganglion mother cells; Hsap\NUMBScer\UAS.PTBS-PRRS clones containing only neurons on the medial surface near the central brain are found at a higher frequency than control clones.
When numbScer\UAS.cWa is driven by Scer\GAL4sca-537.4, bristle formation is inhibited leading to bald patches of cuticle, and the flies die shortly after hatching.
When numbScer\UAS.cWa is driven by Scer\GAL4sca-P309, a strong reduction in external structures on the adult antenna is seen, with a concomitant increase in glial cell numbers, with a concomitant increase in glial cell numbers. The diameter of fascicles in antennal segment 3 are also increased.
Lethality, when driven by Scer\GAL4109-53 or Scer\GAL4109-79 acts in the embryonic-larval stages. When driven by Scer\GAL4sca-109-68 bristle defects occur.
Expression of numbScer\UAS.cWa under the control of Scer\GAL4unspecified in the Malpighian tubules results in two tip cells developing, and the tubules are not arranged normally.
When numbScer\UAS.cWa is driven by Scer\GAL4hs.PB from 3.5 to 7.5 hours after egg laying, Malpighian tubules have two tip cells, instead of one as seen in wild-type. However, there is no change in the final number of tubule cells. The distinction between the number of cells in the anterior and posterior tubules is also unaffected.
The number of eve-expressing pericardial cells is reduced and the number of DA1 muscles is increased in embryos expressing numbScer\UAS.cWa under the control of Scer\GAL4twi.PG.
Embryos expressing numbScer\UAS.cWa under the control of Scer\GAL4sca-537.4 show transformation of sheath cells to neurons in the chordotonal organs. Overexpression of numbScer\UAS.cWa under the control of Scer\GAL4sca-109-68 in adult sensory organs produces a balding phenotype (external sensory organs have no hair and no socket).
Embryos expressing numbScer\UAS.cWa under the control of Scer\GAL4twi.PB show duplications and transformations of muscles, and adult muscle precursors are lost. Muscle VA2 is duplicated at the expense of VA1, DO5 is transformed to LL1 and LT3 is transformed to LT4. The ventral adult muscle precursors are missing and muscle VA3 is duplicated. Lateral and dorsal adult muscle precursors are also missing, and the segment border muscle and dorsal muscles (including DA1) are duplicated. Embryos lack pericardial cells. Despite the muscle aberrations, some embryos develop to form adult flies. These flies are flightless, do not jump and lack ventral abdominal muscles that would normally be generated from the ventral adult muscle precursors.
Scer\GAL4sca-109-68-mediated expression causes twinned hairs and balding. Phenotype is caused by transformation of both the IIa and IIb cell and the sheath to a neuron.
When expression is driven by Scer\GAL4ptc-559.1, at 29oC during the second larval instar stage, adult flies show a loss of distal wing margin at the anterior/posterior boundary. When expressed in the sensory organ precursor cells of the adult external sense organs, using Scer\GAL4sca-109-68, a twinned hair phenotype is produced.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
Enhancer of
Statement
Reference
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Scer\GAL4wor.PA/numbUAS.cWa is a non-suppressor of spindle & neuroblast & larva phenotype of aurA8839
Other
Additional Comments
Genetic Interactions
Statement
Reference
Expression of molScer\UAS.cXa in the wing disc does not enhance the bald notum phenotype seen upon expression of numbScer\UAS.cWa under the control of Scer\GAL4ap-md544.
The addition of numbScer\UAS.cWa driven by Scer\GAL4sca-537.4 to H99/HP8 flies rescues the double socket phenotype seen in the external sensory organs in these flies.
Co-expression of numbScer\UAS.cWa with Su(H)Scer\UAS.cNa driven by Scer\GAL4sca-537.4 results in flies that are partially bald.
The balding phenotype produced by expression of numbScer\UAS.cWa under the control of Scer\GAL4sca-109-68 is enhanced by Df(2L)TW3. The cell fate transformation phenotypes produced by overexpression of either numbScer\UAS.cWa or NakScer\UAS.cCa in the adult sensory organs are suppressed in flies expressing both numbScer\UAS.cWa and NakScer\UAS.cCa in the adult sensory organs under the control of Scer\GAL4sca-109-68.
When expression is driven by Scer\GAL4ptc-559.1 (without increasing the temperature to 29oC) in N55e11/+ flies, an even stronger notched wing phenotype is seen.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Expression of numbScer\UAS.cWa rescues neuronal differentiation defects seen in the outer optic anlage of numb15/numb15 clones in third instar larvae.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
numbScer\UAS.cWa
numbUAS.cWa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Wang
Secondary FlyBase IDs
    References (24)