Allele Dmel\norpA^ninaE.PM

General Information
Symbol	Dmel\norpA^ninaE.PM	Species	D. melanogaster
Name	neither inactivation nor afterpotential E promoter construct of McKay	FlyBase ID	FBal0056441
Feature type	allele	Associated gene	Dmel\norpA

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (McKay et al., 1995, Pearn et al., 1996, van Huizen et al., 1998, Kim et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A ninaE promoter drives expression of the norpA subtype I isoform. (Kim et al., 2003) Full length norpA is expressed under the control of a ninaE promoter. (van Huizen et al., 1998) norpA cDNA sequences from 519 to 4331 (coordinates according to FBrf0047660) are expressed under the control of a ninaE promoter. (Pearn et al., 1996) Construct: norpA cDNA sequences from 519 to 4331 (coordinates according to FBrf0047660) are expressed under the control of a ninaE promoter. (McKay et al., 1995)
Carried in construct	P{ninaE/norpA} (Salcedo et al., 1999, Kim et al., 2003, Malpel et al., 2002, Knox et al., 2003, Cook et al., 2000, McKay et al., 1995, Pearn et al., 1996, van Huizen et al., 1998, Salcedo et al., 2009, Wernet et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	wild-type (van Huizen et al., 1998)
Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference norpA³⁶ ; ninaE¹⁷/+ flies that carry norpA^ninaE.PM have a robust response to light at both 350nm and 520nm (measured using an electroretinogram recording). norpA³⁶ ; ninaE¹⁷/ninaE¹⁷ flies that carry norpA^ninaE.PM have no response to light at both 350nm and 520nm (measured using an ERG recording). Introduction of Lpol\Ops1^ninaE.PK or Lpol\Ops1^{ninaE.T:Btau\1D4} into a norpA³⁶ ; ninaE¹⁷/ninaE¹⁷ ; norpA^ninaE.PM background restores the response to light (measured using an ERG recording), although the response at 520nm is much great than that at 350nm. (Knox et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	norpA^ninaE.PM rescues norpA³⁶ (van Huizen et al., 1998, Kim et al., 2003)
Partially rescues	norpA^ninaE.PM partially rescues norpA³⁶ (Pearn et al., 1996, McKay et al., 1995) norpA^ninaE.PM partially rescues norpA^unspecified (Wernet et al., 2012)
Comments	norpA[ninaE.PM] fails to rescue a polarotactic response to linearly polarised UV light presented dorsally in norpA[unspecified] flies. norpA[ninaE.PM] weakly rescues a polarotactic response to linearly polarised UV light presented ventrally in norpA[unspecified] flies. norpA[ninaE.PM] rescues a polarotactic response to linearly polarised green light presented ventrally in norpA[unspecified] flies. (Wernet et al., 2012) norpA^ninaE.PM flies are able to rescue the physiological defects in norpA³⁶ flies; the rescued flies exhibit electroretinograms (ERGs) that are similar in magnitude and shape to those of control flies. (Kim et al., 2003) norpA^ninaE.PM rescues the electrophysiological phenotype of norpA³⁶ flies in photoreceptor cells R1 to R6, but not in R7 and R8. The degeneration phenotype of the R1 to R6 photoreceptors is also rescued. (Pearn et al., 1996) Partially rescues the reduction in phospholipase C activity seen in the heads of norpA³⁶ flies. Also restores the electroretinogram and prolonged depolarising afterpotential responses of norpA³⁶ flies to a more normal response. (McKay et al., 1995)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	norpA^ninaE.PM
Name Synonym	neither inactivation nor afterpotential E promoter construct of McKay (McKay et al., 1995)
Secondary FlyBase IDs

References ( 10 )
Research paper	Wernet et al., 2012, Curr. Biol. 22(1): 12--20 Genetic dissection reveals two separate retinal substrates for polarization vision in Drosophila. [FBrf0217218] Salcedo et al., 2009, J. Biol. Chem. 284(9): 5717--5722 The Green-absorbing Drosophila Rh6 Visual Pigment Contains a Blue-shifting Amino Acid Substitution That Is Conserved in Vertebrates. [FBrf0207124] Kim et al., 2003, Insect Mol. Biol. 12(2): 147--153 Substitution of a non-retinal phospholipase C in Drosophila phototransduction. [FBrf0159010] Knox et al., 2003, J. Biol. Chem. 278(42): 40493--40502 Heterologous expression of Limulus rhodopsin. [FBrf0174768] Malpel et al., 2002, Development 129(6): 1443--1453 Larval optic nerve and adult extra-retinal photoreceptors sequentially associate with clock neurons during Drosophila brain development. [FBrf0144829] Cook et al., 2000, Nat. Cell Biol. 2(5): 296--301 Phospholipase C and termination of G-protein-mediated signalling in vivo. [FBrf0128818] Salcedo et al., 1999, J. Neurosci. 19(24): 10716--10726 Blue- and green-absorbing visual pigments of Drosophila: ectopic expression and physiological characterization of the R8 photoreceptor cell-specific Rh5 and Rh6 rhodopsins. [FBrf0123183] van Huizen et al., 1998, EMBO J. 17(8): 2285--2297 Two distantly positioned PDZ domains mediate multivalent INAD-phospholipase C interactions essential for G protein-coupled signaling. [FBrf0102437] Pearn et al., 1996, J. Biol. Chem. 271(9): 4937--4945 Molecular, biochemical, and electrophysiological characterization of Drosophila norpA mutants. [FBrf0086575] McKay et al., 1995, J. Biol. Chem. 270(22): 13271--13276 Phospholipase C rescues visual defect in norpA mutant of Drosophila melanogaster. [FBrf0082338]

Allele Dmel\norpAninaE.PM

Allele Dmel\norpA^ninaE.PM