FlyBase Allele Report: Dmel\htl[YY262]

General Information

Symbol

Dmel\htl^YY262

Species

D. melanogaster

Name

FlyBase ID

FBal0057258

Feature type

allele

Associated gene

Dmel\htl

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Michelson et al., 1998)

Mutagen

ethyl methanesulfonate

(Gisselbrecht et al., 1996)

Progenitor genotype

Cytology

Description

Amino acid replacement: E588K. Nucleotide substitution: G1871A.

(Gisselbrecht et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:18,046,114..18,046,114 [-]

Nucleotide change:

G18046114A

Reported nucleotide change:

G1871A

Amino acid change:

E603K | htl-PA; E603K | htl-PB; E603K | htl-PC

Reported amino acid change:

E588K

Comment:

The annotated and reported mutation locations differ by 15aa due to use of a different initiator methione.

(Gisselbrecht et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | embryonic stage

(Schwarz et al., 2018)

Phenotype Manifest In

axon & mechanosensory neuron & adult head (with htl^AB42), with Scer\GAL4^Bx-MS1096, Scer\GAL4^Mz1277, Scer\GAL4^twi.PB, htl^UAS.cMa

(Garcia-Alonso et al., 2000)

axon & ocellus sensory structure (with htl^AB42), with Scer\GAL4^Bx-MS1096, Scer\GAL4^Mz1277, Scer\GAL4^twi.PB, htl^UAS.cMa

(Garcia-Alonso et al., 2000)

embryonic/larval dorsal vessel | embryonic stage

(Gisselbrecht et al., 1996)

embryonic cardioblast

(Schwarz et al., 2018)

embryonic heart ostial cardioblast

(Schwarz et al., 2018)

heart primordium

(Schwarz et al., 2018)

mesoderm

(Mariappa et al., 2011, Michelson et al., 1998)

somatic trunk mesoderm

(Gisselbrecht et al., 1996)

Detailed Description

Statement

Reference

htl^YY262 homozygous embryos exhibit random loss of cardioblasts (both generic cardioblasts and ostial cardioblasts), as compared to controls.

(Schwarz et al., 2018)

Embryos zygotically mutant for htl^YY262 exhibit defects in mesoderm layer formation during gastrulation. Differentiation of eve-positive dorsal mesoderm precursors is also affected in these embryos.

(Mariappa et al., 2011)

htl^YY262/htl^AB42 flies rescued to the pupal stage by expression of htl^Scer\UAS.cMa under the control of three drivers (Scer\GAL4^Mz1277,Scer\GAL4^Bx-MS1096 and Scer\GAL4^twi.PB) gives axon guidance phenotypes in the head. Ocellar pioneer (OP) axons project into the epidermis, bristle mechanosensory (BM) axons fail to project into the brain, or stall in the epidermis or extend apart of epidermis.

(Garcia-Alonso et al., 2000)

Mesodermal cells show some dorsolateral migration in homozygous embryos, but fail to reach the dorsal-most epidermal cells in most segments.

(Michelson et al., 1998)

Significant number of both dorsal somatic muscles and cardiac cells develop.

(Gisselbrecht et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

htl^YY262 has mesoderm phenotype, enhanceable by nst¹⁶⁹²³

(Mariappa et al., 2011)

htl^YY262 has mesoderm phenotype, enhanceable by nst^c04986

(Mariappa et al., 2011)

htl^YY262 has mesoderm phenotype, enhanceable by Df(3L)ED4486

(Mariappa et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

nst¹⁶⁹²³, nst^c04986 or Df(3L)ED4486 zygotically enhance the reduced eve-positive dorsal mesoderm precursor phenotype of htl^YY262 embryos.

(Mariappa et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

htl^EMS2

(Bradley et al., 2003)

htl^J372

(Bradley et al., 2003)

htl^AB42

(Bradley et al., 2003)

Partially rescued by

htl^YY262/htl^AB42 is partially rescued by htl^UAS.cMa/Scer\GAL4^twi.PB/Scer\GAL4^Mz1277/Scer\GAL4^Bx-MS1096

(Garcia-Alonso et al., 2000)

Not rescued by

htl^YY262/htl^AB42 is not rescued by htl^UAS.cMa/Scer\GAL4^twi.PB

(Garcia-Alonso et al., 2000)

Comments

The addition of htl^Scer\UAS.cMa driven by Scer\GAL4^twi.PB fails to rescue the lethality seen in htl^YY262/htl^AB42. However, when htl^Scer\UAS.cMa is driven by Scer\GAL4^Mz1277, Scer\GAL4^Bx-MS1096 and Scer\GAL4^twi.PB concurrently, some escaper pupae do survive.

(Garcia-Alonso et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

htl^YY262

(Schwarz et al., 2018, Mariappa et al., 2011, Garcia-Alonso et al., 2000)

htl^YY26

(Schumacher et al., 2004)

htl^YY

(Mariappa et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

Open Close