Allele Dmel\hh^Scer\UAS.cAa

General Information
Symbol	Dmel\hh^Scer\UAS.cAa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Azpiazu	FlyBase ID	FBal0057274
Feature type	allele	Associated gene	Dmel\hh

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Porter et al., 1996, Azpiazu et al., 1996, Hazelett et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference hh cDNA 11 is cloned as a KpnI-XbaI fragment into pUAST. (Azpiazu et al., 1996) Construct: Expression of full length hh protein is governed by Scer\UAS regulatory sequences. (Porter et al., 1996)
Carried in construct	P{UAS-hh.A} (Menne et al., 1997, Amanai and Jiang, 2001, Chamoun et al., 2001, Rangarajan et al., 2001, Micchelli et al., 2002, Chou and Chien, 2002, Jia et al., 2003, Cadigan et al., 2002, Zhang et al., 2005, Treisman, 2001, Ho et al., 2005, Wang et al., 2000, Lee and Frasch, 2000, Buescher et al., 2004, King et al., 2001, Hazelett et al., 1998, Kango-Singh et al., 2003, Azpiazu et al., 1996, Porter et al., 1996, Zhang et al., 2006, Watson et al., 2011, Suh et al., 2006, Fan et al., 2012, Su et al., 2007, Zheng et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with Scer\GAL4^sim.P3.7 (Watson et al., 2011) visible, with Scer\GAL4^71B (Su et al., 2007)
Phenotype Manifest In
	embryonic dorsal epidermis, with Scer\GAL4^en-e16E (Porter et al., 1996) embryonic ventral epidermis, with Scer\GAL4^en-e16E (Porter et al., 1996) glial cell & eye disc \| somatic clone \| cell non-autonomous, with Scer\GAL4^Act5C.PP (Rangarajan et al., 2001) interfollicle cell \| supernumerary, with Scer\GAL4^hs.PB (King et al., 2001) midline glial cell, with Scer\GAL4^sim.P3.7 (Watson et al., 2011) scutellar bristle, with Scer\GAL4^en-e16E (Porter et al., 1996) wing, with Scer\GAL4^71B (Su et al., 2007) wing vein, with Scer\GAL4^en-e16E (Porter et al., 1996)
Detailed Description
	Statement Reference Midline glia do not completely ensheath the axon commissures, and there is an absence of membrane projections into the commissures in stage 15 embryos expressing hh[Scer\UAS.cAa] under the control of Scer\GAL4[sim.P3.7]. (Watson et al., 2011) Expression of hh[Scer\UAS.cAa] under the control of Scer\GAL4[71B] results in merging of wing veins L2 and L3. (Su et al., 2007) Females with hh^Scer\UAS.cAa, driven by Scer\GAL4^hs.PB (and heatshocked) have an increased number of stalk cells (six-fold more than wild-type). (King et al., 2001) When hh^Scer\UAS.cAa is expressed in clones in the developing eye (under the control of Scer\GAL4^Act5C.PP) a normal number of glial cells is seen in the eye disc and the optic stalk. However glial cells that overshoot past the axonal boundary are seen, usually in streams a few cells wide, and without regard to the position of the clone. (Rangarajan et al., 2001) When expression is driven by Scer\GAL4^en-e16E, only slight differences from wild type are observed in the ventral and dorsal embryonic cuticle pattern. Embryos go on to hatch and form adults that are normal except for wing venation defects and supernumerary scutellar bristles. (Porter et al., 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Scer\GAL4^bi-omb-Gal4, dpp^Scer\UAS.cSa, ey^Scer\UAS.cHa, hh^Scer\UAS.cAa has lethal \| larval stage phenotype (Kango-Singh et al., 2003, Kango-Singh et al., 2003, Kango-Singh et al., 2003)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, ey^Scer\UAS.cHa, hh^Scer\UAS.cAa has wing disc \| somatic clone phenotype, enhanceable by dpp^Scer\UAS.cSa/Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI (Kango-Singh et al., 2003)
NOT Suppressor of
Statement Reference hh^Scer\UAS.cAa/Scer\GAL4^dpp.blk1 is a non-suppressor of morphogenetic furrow phenotype of Scer\GAL4^dpp.blk1, wg^{wg.3'UTR.Scer\UAS} (Cadigan et al., 2002) hh^Scer\UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of kto^T241 (Treisman, 2001) hh^Scer\UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of kto^T555 (Treisman, 2001) hh^Scer\UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of kto^T631 (Treisman, 2001) hh^Scer\UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of skd^T13 (Treisman, 2001) hh^Scer\UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of skd^T413 (Treisman, 2001) hh^Scer\UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of skd^T606 (Treisman, 2001) hh^Scer\UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor cell phenotype of kto^T241 (Treisman, 2001) hh^Scer\UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor cell phenotype of kto^T555 (Treisman, 2001) hh^Scer\UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor cell phenotype of kto^T631 (Treisman, 2001) hh^Scer\UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor cell phenotype of skd^T13 (Treisman, 2001) hh^Scer\UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor cell phenotype of skd^T413 (Treisman, 2001) hh^Scer\UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor cell phenotype of skd^T606 (Treisman, 2001)
Other
Statement Reference Scer\GAL4^30A, ey^Scer\UAS.cHa, hh^Scer\UAS.cAa has eye \| ectopic phenotype (Kango-Singh et al., 2003, Kango-Singh et al., 2003) Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, dpp^Scer\UAS.cSa, ey^Scer\UAS.cHa, hh^Scer\UAS.cAa has wing disc \| ectopic \| somatic clone phenotype (Kango-Singh et al., 2003) Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, ey^Scer\UAS.cHa, hh^Scer\UAS.cAa has eye \| ectopic \| somatic clone phenotype (Kango-Singh et al., 2003, Kango-Singh et al., 2003) Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, ey^Scer\UAS.cHa, hh^Scer\UAS.cAa has wing disc \| somatic clone phenotype (Kango-Singh et al., 2003, Kango-Singh et al., 2003) Scer\GAL4^bi-omb-Gal4, ci^{Ce-2.Scer\UAS.T:Ivir\HA1}, hh^Scer\UAS.cAa has morphogenetic furrow \| ectopic phenotype (Kango-Singh et al., 2003, Kango-Singh et al., 2003) Scer\GAL4^bi-omb-Gal4, ci^{Ce-2.Scer\UAS.T:Ivir\HA1}, hh^Scer\UAS.cAa has wing disc phenotype (Kango-Singh et al., 2003, Kango-Singh et al., 2003) Scer\GAL4^dpp.blk1, hh^Scer\UAS.cAa, wg^{wg.3'UTR.Scer\UAS} has morphogenetic furrow \| ectopic phenotype (Cadigan et al., 2002) Scer\GAL4^dpp.blk1, hh^Scer\UAS.cAa, wg^{wg.3'UTR.Scer\UAS} has morphogenetic furrow phenotype (Cadigan et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference When hh^Scer\UAS.cAa and ey^Scer\UAS.cHa are expressed in clones (under the control of Scer\GAL4^Act5C.PI) in the anterior compartment of the wing disc, 46% of clones cause ectopic eye formation. When hh^Scer\UAS.cAa and ey^Scer\UAS.cHa are expressed under the control of Scer\GAL4^30A in the anterior compartment of the wing disc, ectopic eye formation is seen at 100% penetrance, although the size of these ectopic eyes varies from disc to disc. When ci^{Ce-2.Scer\UAS.T:Ivir\HA1} and hh^Scer\UAS.cAa co-expressed (driven by Scer\GAL4^bi-omb-Gal4) in the wing disc leads to overgrowth and ectopic morphogenetic furrow initiation. When dpp^Scer\UAS.cSa, hh^Scer\UAS.cAa and ey^Scer\UAS.cHa are co-expressed in clones (driven by Scer\GAL4^Act5C.PI), very strong overgrowth and distorted morphology is seen in the wing disc. Co-expression of dpp^Scer\UAS.cSa, hh^Scer\UAS.cAa and ey^Scer\UAS.cHa driven by Scer\GAL4^bi-omb-Gal4, causes a high larval lethality at 25^oC. (Kango-Singh et al., 2003) Co-expression of wg^{wg.3'UTR.Scer\UAS} and hh^Scer\UAS.cAa under the control of Scer\GAL4^dpp.blk1 does not rescue morphogenetic furrow progression from the posterior edge of the eye disc, but usually results in one (or occasionally more) ectopic morphogenetic furrows initiating in the interior of the eye primordium and progressing concentrically. (Cadigan et al., 2002) eyg¹/Df(3L)iro-2 hemizygous eye discs completely lack photoreceptors. Photoreceptor development in these discs is not rescued by hh^Scer\UAS.cAa expressed under the control of Scer\GAL4^dpp.PH. The lack of photoreceptor phenotype of eya¹ eye discs is not rescued by hh^Scer\UAS.cAa expressed under the control of Scer\GAL4^ey.PH. (Hazelett et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	hh^Scer\UAS.cAa, Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF rescues hh^bar3 (Chou and Chien, 2002) Scer\GAL4^en-e16E/hh^Scer\UAS.cAa rescues hh^ts2 (Chamoun et al., 2001)
Comments	hh^Scer\UAS.cAa driven with Scer\GAL4^GMR.PF, rescues morphogenetic furrow progression and ommatidial over-rotation defects in hh^bar3 mutants. (Chou and Chien, 2002)
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Carried in a plasmid and transfected into S2R+ cells. (Zhang et al., 2004)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	hh^Scer\UAS.cAa hh^UAS.cAa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Azpiazu (Azpiazu et al., 1996)
Secondary FlyBase IDs
	FBal0051517
References ( 27 )

Generate a list of	All references relating to this allele ( 27 )

List References by type	Research paper ( 26 ) Personal communication to FlyBase ( 1 )
Recent research papers ( 2 )
	Fan et al., 2012, Dev. Biol. 366(2): 172--184 Hh-induced Smoothened conformational switch is mediated by differential phosphorylation at its C-terminal tail in a dose- and position-dependent manner. [FBrf0218374] Watson et al., 2011, Development 138(7): 1285--1295 Drosophila hedgehog signaling and engrailed-runt mutual repression direct midline glia to alternative ensheathing and non-ensheathing fates. [FBrf0213237] Show all research papers ...

Allele Dmel\hhScer\UAS.cAa

Allele Dmel\hh^Scer\UAS.cAa