|Name||Saccharomyces cerevisiae UAS construct a of Azpiazu||FlyBase ID||FBal0057274|
|Feature type||allele||Associated gene||Dmel\hh|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
(Menne et al., 1997, Amanai and Jiang, 2001, Chamoun et al., 2001, Rangarajan et al., 2001, Micchelli et al., 2002, Chou and Chien, 2002, Jia et al., 2003, Cadigan et al., 2002, Zhang et al., 2005, Treisman, 2001, Ho et al., 2005, Wang et al., 2000, Lee and Frasch, 2000, Buescher et al., 2004, King et al., 2001, Hazelett et al., 1998, Kango-Singh et al., 2003, Azpiazu et al., 1996, Porter et al., 1996, Zhang et al., 2006, Watson et al., 2011, Suh et al., 2006, Fan et al., 2012, Su et al., 2007, Zheng et al., 2010)
|Phenotype Manifest In|
glial cell & eye disc | somatic clone | cell non-autonomous, with Scer\GAL4Act5C.PP
Midline glia do not completely ensheath the axon commissures, and there is an absence of membrane projections into the commissures in stage 15 embryos expressing hh[Scer\UAS.cAa] under the control of Scer\GAL4[sim.P3.7].
Expression of hh[Scer\UAS.cAa] under the control of Scer\GAL4[71B] results in merging of wing veins L2 and L3.
Females with hhScer\UAS.cAa, driven by Scer\GAL4hs.PB (and heatshocked) have an increased number of stalk cells (six-fold more than wild-type).
When hhScer\UAS.cAa is expressed in clones in the developing eye (under the control of Scer\GAL4Act5C.PP) a normal number of glial cells is seen in the eye disc and the optic stalk. However glial cells that overshoot past the axonal boundary are seen, usually in streams a few cells wide, and without regard to the position of the clone.
|Phenotype Manifest In|
|NOT Suppressor of|
hhScer\UAS.cAa/Scer\GAL4dpp.blk1 is a non-suppressor of morphogenetic furrow phenotype of Scer\GAL4dpp.blk1, wgwg.3'UTR.Scer\UAS
Scer\GAL4Act5C.PI/Scer\GAL4Act5C.PI, dppScer\UAS.cSa, eyScer\UAS.cHa, hhScer\UAS.cAa has wing disc | ectopic | somatic clone phenotype
Scer\GAL4Act5C.PI/Scer\GAL4Act5C.PI, eyScer\UAS.cHa, hhScer\UAS.cAa has eye | ectopic | somatic clone phenotype
Scer\GAL4Act5C.PI/Scer\GAL4Act5C.PI, eyScer\UAS.cHa, hhScer\UAS.cAa has wing disc | somatic clone phenotype
Scer\GAL4bi-omb-Gal4, ciCe-2.Scer\UAS.T:Ivir\HA1, hhScer\UAS.cAa has morphogenetic furrow | ectopic phenotype
When hhScer\UAS.cAa and eyScer\UAS.cHa are expressed in clones (under the control of Scer\GAL4Act5C.PI) in the anterior compartment of the wing disc, 46% of clones cause ectopic eye formation. When hhScer\UAS.cAa and eyScer\UAS.cHa are expressed under the control of Scer\GAL430A in the anterior compartment of the wing disc, ectopic eye formation is seen at 100% penetrance, although the size of these ectopic eyes varies from disc to disc. When ciCe-2.Scer\UAS.T:Ivir\HA1 and hhScer\UAS.cAa co-expressed (driven by Scer\GAL4bi-omb-Gal4) in the wing disc leads to overgrowth and ectopic morphogenetic furrow initiation. When dppScer\UAS.cSa, hhScer\UAS.cAa and eyScer\UAS.cHa are co-expressed in clones (driven by Scer\GAL4Act5C.PI), very strong overgrowth and distorted morphology is seen in the wing disc. Co-expression of dppScer\UAS.cSa, hhScer\UAS.cAa and eyScer\UAS.cHa driven by Scer\GAL4bi-omb-Gal4, causes a high larval lethality at 25oC.
Co-expression of wgwg.3'UTR.Scer\UAS and hhScer\UAS.cAa under the control of Scer\GAL4dpp.blk1 does not rescue morphogenetic furrow progression from the posterior edge of the eye disc, but usually results in one (or occasionally more) ectopic morphogenetic furrows initiating in the interior of the eye primordium and progressing concentrically.
eyg1/Df(3L)iro-2 hemizygous eye discs completely lack photoreceptors. Photoreceptor development in these discs is not rescued by hhScer\UAS.cAa expressed under the control of Scer\GAL4dpp.PH. The lack of photoreceptor phenotype of eya1 eye discs is not rescued by hhScer\UAS.cAa expressed under the control of Scer\GAL4ey.PH.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
Carried in a plasmid and transfected into S2R+ cells.
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of Azpiazu
|Secondary FlyBase IDs|
|References ( 27 )|
|Generate a list of|
|List References by type|
|Recent research papers ( 2 )|