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General Information
Symbol
Dmel\hhUAS.cAa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Azpiazu
FlyBase ID
FBal0057274
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-hh
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of hh cDNA 11.

Expression of full length hh protein is governed by UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing hhUAS.cAa under the control of Scer\GAL4C587 leads to follicle cell defects.

Midline glia do not completely ensheath the axon commissures, and there is an absence of membrane projections into the commissures in stage 15 embryos expressing hhScer\UAS.cAa under the control of Scer\GAL4sim.P3.7.

Expression of hhScer\UAS.cAa under the control of Scer\GAL471B results in merging of wing veins L2 and L3.

Females with hhScer\UAS.cAa, driven by Scer\GAL4hs.PB (and heatshocked) have an increased number of stalk cells (six-fold more than wild-type).

When hhScer\UAS.cAa is expressed in clones in the developing eye (under the control of Scer\GAL4Act5C.PP) a normal number of glial cells is seen in the eye disc and the optic stalk. However glial cells that overshoot past the axonal boundary are seen, usually in streams a few cells wide, and without regard to the position of the clone.

When expression is driven by Scer\GAL4en-e16E, only slight differences from wild type are observed in the ventral and dorsal embryonic cuticle pattern. Embryos go on to hatch and form adults that are normal except for wing venation defects and supernumerary scutellar bristles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
NOT Suppressor of
Statement
Reference

hhUAS.cAa/Scer\GAL4ey.PH is a non-suppressor of eye disc phenotype of skdT13

hhUAS.cAa/Scer\GAL4ey.PH is a non-suppressor of photoreceptor cell phenotype of skdT13

hhUAS.cAa/Scer\GAL4ey.PH is a non-suppressor of eye disc phenotype of ktoT631

hhUAS.cAa/Scer\GAL4ey.PH is a non-suppressor of eye disc phenotype of ktoT241

hhUAS.cAa/Scer\GAL4ey.PH is a non-suppressor of eye disc phenotype of skdT413

hhUAS.cAa/Scer\GAL4ey.PH is a non-suppressor of eye disc phenotype of ktoT555

hhUAS.cAa/Scer\GAL4ey.PH is a non-suppressor of eye disc phenotype of skdT606

Other
Additional Comments
Genetic Interactions
Statement
Reference

When hhScer\UAS.cAa and eyScer\UAS.cHa are expressed in clones (under the control of Scer\GAL4Act5C.PI) in the anterior compartment of the wing disc, 46% of clones cause ectopic eye formation. When hhScer\UAS.cAa and eyScer\UAS.cHa are expressed under the control of Scer\GAL430A in the anterior compartment of the wing disc, ectopic eye formation is seen at 100% penetrance, although the size of these ectopic eyes varies from disc to disc. When ciCe-2.Scer\UAS.T:Ivir\HA1 and hhScer\UAS.cAa co-expressed (driven by Scer\GAL4bi-omb-Gal4) in the wing disc leads to overgrowth and ectopic morphogenetic furrow initiation. When dppScer\UAS.cSa, hhScer\UAS.cAa and eyScer\UAS.cHa are co-expressed in clones (driven by Scer\GAL4Act5C.PI), very strong overgrowth and distorted morphology is seen in the wing disc. Co-expression of dppScer\UAS.cSa, hhScer\UAS.cAa and eyScer\UAS.cHa driven by Scer\GAL4bi-omb-Gal4, causes a high larval lethality at 25oC.

Co-expression of wgwg.3'UTR.Scer\UAS and hhScer\UAS.cAa under the control of Scer\GAL4dpp.blk1 does not rescue morphogenetic furrow progression from the posterior edge of the eye disc, but usually results in one (or occasionally more) ectopic morphogenetic furrows initiating in the interior of the eye primordium and progressing concentrically.

eyg1/Df(3L)iro-2 hemizygous eye discs completely lack photoreceptors. Photoreceptor development in these discs is not rescued by hhScer\UAS.cAa expressed under the control of Scer\GAL4dpp.PH. The lack of photoreceptor phenotype of eya1 eye discs is not rescued by hhScer\UAS.cAa expressed under the control of Scer\GAL4ey.PH.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

hhScer\UAS.cAa driven with Scer\GAL4GMR.PF, rescues morphogenetic furrow progression and ommatidial over-rotation defects in hhbar3 mutants.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Carried in a plasmid and transfected into S2R+ cells.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
hhScer\UAS.cAa
hhUAS.cAa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Azpiazu
Secondary FlyBase IDs
  • FBal0051517
References (32)