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General Information
Symbol
Dmel\shgi.UAS.Tag:SS(aos),Tag:MYC
Species
D. melanogaster
Name
intracellular
FlyBase ID
FBal0057352
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Cadintra
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of a dominant negative form of shg (consists of the C-terminal 208 amino acids, which encode the transmembrane and cytoplasmic domains). The protein is tagged at the N-terminal end with the Tag:SS(aos) signal sequence and is also tagged with Tag:MYC.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
model of  cancer
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
The expression of shgi.Scer\UAS.T:SS-aos,T:Hsap\MYC under the control of Scer\GAL4dpp.PU in third instar larval wing disc cells results in some expressing cells migrating and crossing the anterior-posterior compartment border; the clonal expression of shgi.Scer\UAS.T:SS-aos,T:Hsap\MYC under the control of Scer\GAL4Act5C.PI leads to expressing cells migrating and scattering across long distances in the wing disc, and frequently exhibiting long actin rich protrusions, as compared to controls.
Expression of aos::shgi.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4en.PU results in loss of wing blade.
When argos::shgi.Scer\UAS.T:Hsap\MYC is driven by Scer\GAL4pnr-MD237, abnormal patterning of the notal microchaetae is seen. Instead of being aligned in five straight rows, as seen in wild-type, they are arranged chaotically. The density of bristles is also increased. When argos::shgi.Scer\UAS.T:Hsap\MYC is driven by Scer\GAL4neur-GAL4-A101 or Scer\GAL4sca-537.4 no effect is seen on bristle pattern.
Overexpression of argos::shgi.Scer\UAS.T:Hsap\MYC in all cells of the medial half of the notum, results in a slight decrease in bristle density.
When argos::shgi.Scer\UAS.T:Hsap\MYC is driven by Scer\GAL4en-e16E wing margin defects are seen in the adult wing.
Expression of argos::shgi.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4en-e16E results in posterior wing notching, mimicking wg hypomorphic mutants. The strength of the wing notching phenotype caused by expression of argos::shgi.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4en-e16E varies depending on the particular P{UAS-shg.CADi} insertion used. Flies carrying the P{UAS-shg.CADi}10 insertion expressed under the control of Scer\GAL4en-e16E always retain the posterior crossvein and a tuft of marginal bristles between the tips of wing veins 4 and 5, while these structures are consistently absent in flies carrying the P{UAS-shg.CADi}5 insertion expressed under the control of Scer\GAL4en-e16E. Expression of argos::shgi.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF results in rough eyes. Extra interommatidial bristles are produced and two bristles can often be seen originating from a single socket.
Scer\GAL4arm.T:Hsim\VP16 mediated expression causes head and dorsal defects. Scer\GAL4en-e16E mediated expression causes loss of wing margin in the posterior compartment. Scer\GAL4arm.PS mediated expression causes defects in adult appendages; notum duplications or a drastic reduction of the wing to a tiny winglet.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
NOT Suppressor of
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Additional Comments
Genetic Interactions
Statement
Reference
The loss of wing blade caused by expression of aos::shgi.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4en.PU is partially suppressed by Traf43/+.
The severity of the serrated wing margin phenotype in argos::shgi.Scer\UAS.T:Hsap\MYC; Scer\GAL4vg.PM flies is enhanced by twsj11C8/+.
The metastatic behaviour seen in clones in the eye disc which are homozygous for scrib1 and are also expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act5C.PI is not suppressed if they are co-expressing argos::shgi.Scer\UAS.T:Hsap\MYC.
Overexpression of argos::shgi.Scer\UAS.T:Hsap\MYC in all cells of the medial half of the notum, results in a slight increase in bristle density in a scaBP2 mutant background.
The addition of pont5.1 enhances the wing margin phenotype seen in argos::shgi.Scer\UAS.T:Hsap\MYC, Scer\GAL4en-e16E flies. The addition of arm4 strongly enhances the wing margin phenotype seen in argos::shgi.Scer\UAS.T:Hsap\MYC, Scer\GAL4en-e16E flies. The addition of rept35 suppresses the wing margin phenotype seen in argos::shgi.Scer\UAS.T:Hsap\MYC, Scer\GAL4en-e16E flies.
The wing notching phenotype caused by expression of argos::shgi.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4en-e16E is enhanced by wgl-17, arm1, arm2 or dshb117. The wing notching phenotype caused by expression of argos::shgi.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4en-e16E is suppressed by sggM11. The rough eye phenotype caused by expression of argos::shgi.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF is enhanced by wgl-17 and dshb117 and suppressed by sggM11. The wing notching phenotype caused by expression of argos::shgi.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4en-e16E is rescued by co-expression of armΔα.Scer\UAS though not by co-expression of Hsap\JUPScer\UAS.cWa.
Ectopic expression of armScer\UAS.cSa rescues the wing defect caused by expression of argos::shgi.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4en-e16E to wild type. Loss of one copy of arm (arm1) enhances the wing phenotype of surviving flies expressing argos::shgi.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4en-e16E, most die as pupae.
Xenogenetic Interactions
Statement
Reference
The wing notching phenotype caused by expression of argos::shgi.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4en-e16E is not rescued by co-expression of Hsap\JUPScer\UAS.cWa.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
Reported As
Symbol Synonym
UASCadintra
aos::shgi.Scer\UAS.T:Hsap\MYC
argos::shgi.Scer\UAS.T:Hsap\MYC
argos::shgi.UAS.T:Myc
gil::shgi.UAS.T:Myc
shgi.Scer\UAS.T:SS-aos,T:Hsap\MYC
shgi.UAS.Tag:SS(aos),Tag:MYC
Name Synonyms
intracellular
Secondary FlyBase IDs
    References (12)