FlyBase Allele Report: Dmel\ftz-f1[hs.PM]

General Information

Symbol

Dmel\ftz-f1^hs.PM

Species

D. melanogaster

Name

heat shock construct of Murata

FlyBase ID

FBal0057393

Feature type

allele

Associated gene

Dmel\ftz-f1

Associated Insertion(s)

Carried in Construct

P{hsLFTZ-F1}

Also Known As

hs-βFTZ-F1, hsΒFTZ-F1

Key Links

Transgenic product class

genomic_DNA

(Murata et al., 1996)

wild_type

(Murata et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Murata et al., 1996)

wild_type

(Murata et al., 1996)

Mutagen

in vitro construct

(Murata et al., 1996)

Progenitor genotype

Carried in construct

P{hsLFTZ-F1}

Cytology

Description

Hsp70 promoter drives expression of a genomic fragment of ftz-f1.

(Murata et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

ftz-f1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | prepupal stage | heat sensitive

(Akagi et al., 2016)

lethal | heat sensitive

(Yamada et al., 2000)

Phenotype Manifest In

cephalopharyngeal skeleton | heat sensitive

(Yamada et al., 2000)

cephalopharyngeal skeleton | heat sensitive, with ftz-f1^ex7

(Yamada et al., 2000)

embryonic/larval anterior spiracle | heat sensitive

(Yamada et al., 2000)

embryonic/larval anterior spiracle | heat sensitive, with ftz-f1^ex7

(Yamada et al., 2000)

embryonic/larval cuticle | heat sensitive

(Yamada et al., 2000)

embryonic/larval cuticle | heat sensitive, with ftz-f1^ex7

(Yamada et al., 2000)

embryonic/larval posterior spiracle | heat sensitive

(Yamada et al., 2000)

embryonic/larval posterior spiracle | heat sensitive, with ftz-f1^ex7

(Yamada et al., 2000)

epicuticle | heat sensitive

(Yamada et al., 2000)

larval tracheal system | heat sensitive

(Yamada et al., 2000)

larval tracheal system | heat sensitive, with ftz-f1^ex7

(Yamada et al., 2000)

mouth hook | heat sensitive

(Yamada et al., 2000)

mouth hook | heat sensitive, with ftz-f1^ex7

(Yamada et al., 2000)

pupal endocuticle | heat sensitive

(Yamada et al., 2000)

Detailed Description

Statement

Reference

Using the heat-inducible ftz-f1^hs.PM line, pupation timing is significantly advanced by around 30min when heat shock is received at 6hr APF (no advancement is seen when animals are exposed at 7hr APF or later).

In ftz-f1^ex7/ftz-f1⁰³⁶⁴⁹ mutants, no pupation occurs; when ftz-f1^hs.PM is induced at 8hr APF or later, pupation occurs and is delayed (length of delay depends on the timing of the heat shock).

(Akagi et al., 2016)

Expression of ftz-f1^hs.PM in a wild-type background using heat shock at or around 36 or 60 hours after egg laying results in a drastic decrease in the number of animals that survive to adulthood. Animals that are heat shocked during the critical period can survive for at least a few days, but they never progress to the next larval instar stage. The larvae have two pairs of mouth hooks, cephalopharyngeal skeletons, anterior and posterior spiracles and double tubular tracheae, and cuticle for the third instar is present in addition to that for the second instar 24 hours after heat shock. The cuticle consists of two sets of epicuticle layers and lamellar endocuticle layers. The epicuticle layer is not even and in some cases is discontinuous. Expression of ftz-f1^hs.PM in a wild-type background during the mid- to late prepupal preiod and early pupal period has no effect on development. Heat treatment in the period between 0-4 hours after puparium formation arrests development before stage P4.

(Yamada et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ftz-f1^hs.PM is a suppressor of lethal | pupal stage phenotype of Cyp18a1^GD1176, Scer\GAL4^da.G32

(Rewitz et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

ftz-f1^hs.PM is a suppressor of leg | pupal stage phenotype of Cyp18a1^GD1176, Scer\GAL4^da.G32

(Rewitz et al., 2010)

ftz-f1^hs.PM is a suppressor of leg | pupal stage phenotype of Cyp18a1^GD1176, Scer\GAL4^da.G32, Scer\GAL80^ts.αTub84B

(Rewitz et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Induction of expression of ftz-f1^hs.PM at 25[o]C in animals expressing Cyp18a1^GD1176 under the control of Scer\GAL4^da.G32 rescues lethality and leg abnormalities.

Induction of expression of ftz-f1^hs.PM at 25[o]C in animals expressing Cyp18a1^GD1176 under the control of Scer\GAL4^da.G32 and Scer\GAL80^ts.αTub84B rescues the leg malformation phenotype. Only 12% of animals display any leg defects, compared to 87% of control animals.

(Rewitz et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

ftz-f1^hs.PM partially rescues ftz-f1⁰³⁶⁴⁹/ftz-f1^ex7

(Akagi et al., 2016)

ftz-f1^hs.PM partially rescues ftz-f1⁰³⁶⁴⁹/ftz-f1^ex7

(Yamada et al., 2000)

ftz-f1^hs.PM partially rescues ftz-f1^ex7

(Yamada et al., 2000)

Comments

In ftz-f1^ex7/ftz-f1⁰³⁶⁴⁹ mutants, no pupation occurs when ftz-f1^hs.PM is induced at 4 or 5hr APF, but 75 or 100% of animals become pupa at 12.3 or 12.6 APF on average when ftz-f1^hs.PM is induced at 6 or 7hr APF, respectively.

(Akagi et al., 2016)

About 40% of ftz-f1^ex7 homozygous embryos hatch when ftz-f1^hs.PM is expressed using heat shock at 13-16 hours after egg laying. ftz-f1^ex7 lethality is not rescued by heat shock at other times. ftz-f1^hs.PM rescues ftz-f1^ex7/ftz-f1⁰³⁶⁴⁹ animals that are arrested as prepupae before stage P4; the rescued animals are able to become pupae and a fraction of them develop until at least stage P12. Efficient rescue is seen when the animals are heat shocked 6-10 hours after puparium formation.

(Yamada et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ftz-f1^hs.PM

Name Synonyms

heat shock construct of Murata

(Murata et al., 1996)

Secondary FlyBase IDs

References (10)

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