Allele Dmel\dap^Scer\UAS.cLa

General Information
Symbol	Dmel\dap^Scer\UAS.cLa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Lane	FlyBase ID	FBal0057654
Feature type	allele	Associated gene	Dmel\dap

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Lane et al., 1996, Calvi et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\GAL4 Scer\UAS binding sites are fused upstream of dap cDNA sequences. (Lane et al., 1996)
Carried in construct	P{UAS-dap.L} (Han and Bodmer, 2003, Weiss et al., 1998, Calvi et al., 1998, Audibert et al., 2005, Szuplewski et al., 2003, Delanoue et al., 2002, Remaud et al., 2008, Duman-Scheel et al., 2004, Duman-Scheel et al., 2002, Wang et al., 2006, Berger et al., 2005, Lane et al., 1996, Legent et al., 2006, Shibutani et al., 2007, Ninov et al., 2007, Brun et al., 2006, van de Bor et al., 1999, Zielke et al., 2006, Delanoue et al., 2004, Gupta et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile, with Scer\GAL4^c323 (Calvi et al., 1998) mitotic cell cycle defective, with Scer\GAL4^neur-P72 (Audibert et al., 2005) neuroanatomy defective, with Scer\GAL4^sca-537.4 (Berger et al., 2005) size defective, with Scer\GAL4^ey.PH (Gupta et al., 2008) visible, with Scer\GAL4^GMR.PF (Gupta et al., 2008) visible, with Scer\GAL4^ptc-559.1 (Legent et al., 2006) visible, with Scer\GAL4^vg.int2.1 (Legent et al., 2006)
Phenotype Manifest In
	adult abdomen \| somatic clone, with Scer\GAL4^Act5C.PI (Ninov et al., 2007) cardioblast, with Scer\GAL4^twi.PG (Han and Bodmer, 2003) chorion, with Scer\GAL4^c323 (Calvi et al., 1998) dorsal appendage, with Scer\GAL4^c323 (Calvi et al., 1998) egg, with Scer\GAL4^c323 (Calvi et al., 1998) embryonic/larval epidermis \| somatic clone, with Scer\GAL4^Act5C.PI (Ninov et al., 2007) embryonic pericardial cell, with Scer\GAL4^eve.eme (Han and Bodmer, 2003) embryonic pericardial cell, with Scer\GAL4^twi.PG (Han and Bodmer, 2003) eye, with Scer\GAL4^GMR.PF (Gupta et al., 2008) eye disc posterior to the morphogenetic furrow \| somatic clone, with Scer\GAL4^GMR.PU (Zielke et al., 2006) G1 phase & external sensory organ precursor cell IIa, with Scer\GAL4^neur-P72 (Audibert et al., 2005) G1 phase & external sensory organ precursor cell IIb, with Scer\GAL4^neur-P72 (Audibert et al., 2005) histoblast \| somatic clone, with Scer\GAL4^Act5C.PI (Ninov et al., 2007) neuroblast NB6-4 of thorax, with Scer\GAL4^sca-537.4 (Berger et al., 2005) neuron & embryonic thorax, with Scer\GAL4^sca-537.4 (Berger et al., 2005) wing, with Scer\GAL4^vg.int2.1 (Legent et al., 2006) wing disc, with Scer\GAL4^ptc-559.1 (Legent et al., 2006) wing margin, with Scer\GAL4^ptc-559.1 (Legent et al., 2006)
Detailed Description
	Statement Reference Expression of dap[Scer\UAS.cLa] under the control of Scer\GAL4[GMR.PF] results in a small eye phenotype. (Gupta et al., 2008) S-phase in secondary precursor cells in the bristle lineage is delayed by around 20 minutes by expression of dap[Scer\UAS.cLa] under the control of Scer\GAL4[neur-P72]. (Remaud et al., 2008) Histoblast clones that express dap^Scer\UAS.cLa under the control of Scer\GAL4^Act5C.PI do not proliferate. This results in a profound delay in larval epidermis cell death. Developmental timing is not affected in these animals but pharate adult esacpers show multiple differentiation and morphogenetic abdominal defects. (Ninov et al., 2007) The distance between wing veins L3 and L4 at the wing margin is reduced in animals expressing dap^Scer\UAS.cLa under the control of Scer\GAL4^ptc-559.1. There is a decrease in S phase cells in cells expressing dap^Scer\UAS.cLa under the control of Scer\GAL4^ptc-559.1 in the wing disc. Expression of dap^Scer\UAS.cLa under the control of Scer\GAL4^vg.int2.1 results in nicks in the wing. (Legent et al., 2006) Over-expression of dap[Scer\UAS.cLa] under the control of Scer\GAL4[GMR.PU] blocks the second wave of mitosis posterior to the morphogenetic furrow in the eye imaginal disc. (Zielke et al., 2006) Overexpression of dap^Scer\UAS.cLa under the control of Scer\GAL4^neur-P72 triggers a G1-phase in pIIa and pIIb cells. (Audibert et al., 2005) Overexpression of dap^Scer\UAS.cLa, under the regulation of Scer\GAL4^sca-537.4 results in a reduction in the number of neurons in the NB6-4t lineage (from the wild-type number of 6 to 2-4 in approximately 81% of hemisegments), but homeotic transformation of the lineage does not occur. In addition, the number of glial cells is not affected, neither in the thorax nor in the abdomen. (Berger et al., 2005) When dap^Scer\UAS.cLa is driven by Scer\GAL4^twi.PG, most hemisegments of mutant embryos show only one eve expressing pericardial cell (EPC), suggesting that the last division generating two EPCs did not occur. A reduction is also seen in the number of myocardial cells in these mutants. When dap^Scer\UAS.cLa is driven by Scer\GAL4^eve.eme, both DA1 and DO2 founders form, but the number of EPCs is reduced to half. (Han and Bodmer, 2003) Females expressing dap^Scer\UAS.cLa under the control of Scer\GAL4^c323 are sterile, and lay flaccid eggs with thin eggshells and smaller dorsal appendages than normal. (Calvi et al., 1998) Scer\GAL4^F4-mediated expression results in inhibition of endoreplication. (Weiss et al., 1998) Scer\GAL4^prd.RG1 mediated overexpression results in a stable G1 arrest. (Lane et al., 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^vg.int2.1, dap^Scer\UAS.cLa has visible phenotype, enhanceable by vg[+]/vg^null (Legent et al., 2006)
Suppressed by
Statement Reference Scer\GAL4^vg.int2.1, dap^Scer\UAS.cLa, vg[+]/vg^null has visible phenotype, suppressible by vg^Scer\UAS.cZa/Scer\GAL4^vg.int2.1 (Delanoue et al., 2004)
Enhancer of
Statement Reference dap^Scer\UAS.cLa, Scer\GAL4^ey.PH is an enhancer of size defective phenotype of Mmus\Shisa5^Scer\UAS.cGa, Scer\GAL4^ey.PH (Gupta et al., 2008) dap^Scer\UAS.cLa, Scer\GAL4^ey.PH is an enhancer of visible phenotype of Mmus\Shisa5^Scer\UAS.cGa, Scer\GAL4^ey.PH (Gupta et al., 2008)
Suppressor of
Statement Reference dap^Scer\UAS.cLa/Scer\GAL4^wor.PA is a suppressor of neuroanatomy defective phenotype of aur⁸⁸³⁹ (Wang et al., 2006)
NOT Suppressor of
Statement Reference dap^Scer\UAS.cLa/Scer\GAL4^vg.PM is a non-suppressor of lethal \| pupal stage phenotype of Scer\GAL4^vg.PM, stwl^UY823 (Brun et al., 2006)
Other
Statement Reference Scer\GAL4^vg.int2.1, dap^Scer\UAS.cLa, vg[+]/vg^null has visible phenotype (Delanoue et al., 2004) Scer\GAL4^vg.PU, dap^Scer\UAS.cLa, vg[+]/vg^null has visible phenotype (van de Bor et al., 1999)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^vg.int2.1, dap^Scer\UAS.cLa has wing phenotype, enhanceable by vg[+]/vg^null (Legent et al., 2006)
Suppressed by
Statement Reference Scer\GAL4^prd.RG1, dap^Scer\UAS.cLa has cell cycle phenotype, suppressible by CycE^Scer\UAS.cLa, Scer\GAL4^prd.RG1 (Lane et al., 1996) Scer\GAL4^vg.int2.1, dap^Scer\UAS.cLa, vg[+]/vg^null has wing phenotype, suppressible by vg^Scer\UAS.cZa/Scer\GAL4^vg.int2.1 (Delanoue et al., 2004)
Enhancer of
Statement Reference dap^Scer\UAS.cLa, Scer\GAL4^ey.PH is an enhancer of eye phenotype of Mmus\Shisa5^Scer\UAS.cGa, Scer\GAL4^ey.PH (Gupta et al., 2008)
Suppressor of
Statement Reference dap^Scer\UAS.cLa/Scer\GAL4^Act5C.PP is a suppressor of morphogenetic furrow \| somatic clone phenotype of Scer\GAL4^Act5C.PP, ci^Scer\UAS.cDa (Duman-Scheel et al., 2002) dap^Scer\UAS.cLa/Scer\GAL4^Act5C.PP is a suppressor of S phase \| ectopic \| somatic clone phenotype of Scer\GAL4^Act5C.PP, ci^Scer\UAS.cDa (Duman-Scheel et al., 2002) dap^Scer\UAS.cLa/Scer\GAL4^wor.PA is a suppressor of neuroblast \| supernumerary & larval brain phenotype of aur⁸⁸³⁹ (Wang et al., 2006)
NOT Suppressor of
Statement Reference dap^Scer\UAS.cLa, Scer\GAL4^GMR.PU is a non-suppressor of eye disc posterior to the morphogenetic furrow \| somatic clone phenotype of Rca1^{Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG}, Scer\GAL4^GMR.PU (Zielke et al., 2006)
Other
Statement Reference Scer\GAL4^vg.int2.1, dap^Scer\UAS.cLa, vg[+]/vg^null has wing phenotype (Delanoue et al., 2004) Scer\GAL4^vg.PU, dap^Scer\UAS.cLa, vg[+]/vg^null has wing phenotype (van de Bor et al., 1999)
Additional Comments
Genetic Interactions
Statement Reference The severity of the wing nicking phenotype caused by expression of dap^Scer\UAS.cLa under the control of Scer\GAL4^vg.int2.1 is greatly enhanced by vg^null/+. (Legent et al., 2006) Over-expression of Rca1[Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG] and dap[Scer\UAS.cLa] under the control of Scer\GAL4[GMR.PU] does not alter the ability of Rca1[Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG] to induce ectopic cell division in the eye disc. (Zielke et al., 2006) Expression of dap[Scer\UAS.cLa] along the dorsal/ventral boundary of the wing under the control of Scer\GAL4[vg.int2.1] in a vg[null]/+ mutant background results in nicks at the wing tips. There is a significant decrease in mitotic cells along the dorsal/ventral boundary. Co-expression of vg[Scer\UAS.cZa] in flies expressing dap[Scer\UAS.cLa] at the dorsal/ventral wing boundary under the control of Scer\GAL4[vg.int2.1] in a vg[null]/+ mutant background rescues wing morphology, and mitotic activity of cells is restored in this region. (Delanoue et al., 2004) Co-expression of dap^Scer\UAS.cLa blocks the ability of ci^Scer\UAS.cDa (expressed under the control of Scer\GAL4^Act5C.PP) to induce S phase in clones in the morphogenetic furrow. (Duman-Scheel et al., 2002) Expression of dap[Scer\UAS.cLa] under the control of Scer\GAL4[vg.PU] in a vg[null]/+ background results in a mutant wing phenotype. (van de Bor et al., 1999) Simultaneous expression of CycE^Scer\UAS.cLa completely rescues the cell cycle arrest induced by Scer\GAL4^prd.RG1 mediated overexpression of dap^Scer\UAS.cLa. (Lane et al., 1996)
Xenogenetic Interactions
Statement Reference Expression of dap[Scer\UAS.cLa] greatly enhances the eye phenotypes seen when Mmus\Shisa5[Scer\UAS.cGa] is expressed under the control of Scer\GAL4[ey.PH]. (Gupta et al., 2008)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Incorporation of BrdU is undetectable in virtually all follicle cells in egg chambers of females expressing dap^Scer\UAS.cLa under the control of Scer\GAL4^c323 or Scer\GAL4^hs.PB. (Calvi et al., 1998)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	dap^Scer\UAS.cLa dap^UAS.cLa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Lane (Lane et al., 1996)
Secondary FlyBase IDs

References ( 20 )
Research paper	Gupta et al., 2008, Cell Cycle 7(15): 2401--2408 Cell cycle regulation by the pro-apoptotic gene Scotin. [FBrf0205659] Remaud et al., 2008, PLoS ONE 3(11): e3646 S-phase favours notch cell responsiveness in the Drosophila bristle lineage. [FBrf0206232] Ninov et al., 2007, Development 134(2): 367--379 Extrinsic and intrinsic mechanisms directing epithelial cell sheet replacement during Drosophila metamorphosis. [FBrf0194691] Shibutani et al., 2007, Development 134(3): 467--478 Rbf1-independent termination of E2f1-target gene expression during early Drosophila embryogenesis. [FBrf0192129] Brun et al., 2006, Cell Death Differ. 13(10): 1752--1762 The myb-related gene stonewall induces both hyperplasia and cell death in Drosophila: rescue of fly lethality by coexpression of apoptosis inducers. [FBrf0193692] Legent et al., 2006, Genes Cells 11(8): 907--918 Cell cycle genes regulate vestigial and scalloped to ensure normal proliferation in the wing disc of Drosophila melanogaster. [FBrf0193006] Wang et al., 2006, Genes Dev. 20(24): 3453--3463 Aurora-A acts as a tumor suppressor and regulates self-renewal of Drosophila neuroblasts. [FBrf0194325] Zielke et al., 2006, EMBO Rep. 7(12): 1266--1272 Molecular dissection of the APC/C inhibitor Rca1 shows a novel F-box-dependent function. [FBrf0193300] Audibert et al., 2005, Development 132(10): 2287--2297 Cell cycle diversity involves differential regulation of Cyclin E activity in the Drosophila bristle cell lineage. [FBrf0187449] Berger et al., 2005, Nat. Cell Biol. 7(1): 56--62 A critical role for Cyclin E in cell fate determination in the central nervous system of Drosophila melanogaster. [FBrf0184186] Delanoue et al., 2004, Cell Death Differ. 11(1): 110--122 The Drosophila wing differentiation factor Vestigial-Scalloped is required for cell proliferation and cell survival at the dorso-ventral boundary of the wing imaginal disc. [FBrf0167376] Duman-Scheel et al., 2004, Proc. Natl. Acad. Sci. U.S.A. 101(11): 3857--3862 Repression of dMyc expression by Wingless promotes Rbf-induced G1 arrest in the presumptive Drosophila wing margin. [FBrf0175090] Han and Bodmer, 2003, Development 130(13): 3039--3051 Myogenic cells fates are antagonized by Notch only in asymmetric lineages of the Drosophila heart, with or without cell division. [FBrf0160590] Szuplewski et al., 2003, Development 130(16): 3651--3662 The Drosophila bZIP transcription factor Vrille is involved in hair and cell growth. [FBrf0160992] Delanoue et al., 2002, Genes Cells 7(12): 1255--1266 Interaction between apterous and early expression of vestigial in formation of the dorso-ventral compartments in the Drosophila wing disc. [FBrf0155789] Duman-Scheel et al., 2002, Nature 417(6886): 299--304 Hedgehog regulates cell growth and proliferation by inducing Cyclin D and Cyclin E. [FBrf0149123] van de Bor et al., 1999, Cell Death Differ. 6(6): 557--564 Truncated products of the vestigial proliferation gene induce apoptosis. [FBrf0111532] Calvi et al., 1998, Genes Dev. 12(5): 734--744 Cell cycle control of chorion gene amplification. [FBrf0101877] Weiss et al., 1998, Curr. Biol. 8(4): 239--242 Continuous Cyclin E expression inhibits progression through endoreduplication cycles in Drosophila. [FBrf0100714] Lane et al., 1996, Cell 87(7): 1225--1235 Dacapo, a cyclin-dependent kinase inhibitor, stops cell proliferation during Drosophila development. [FBrf0091107]

Allele Dmel\dapScer\UAS.cLa

Allele Dmel\dap^Scer\UAS.cLa