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General Information
Symbol
Dmel\dapUAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Lane
FlyBase ID
FBal0057654
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Dap, UAS-Dacapo
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of dap cDNA sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
ameliorates  brain cancer
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Overexpression of dapScer\UAS.cLa in the imaginal discs, under the control of Scer\GAL4en.PU or Scer\GAL4hh.PU, leads to G[[1]] cell cycle arrest.

Expression of dapScer\UAS.cLa under the control of Scer\GAL4GMR.PF results in a small eye phenotype.

S-phase in secondary precursor cells in the bristle lineage is delayed by around 20 minutes by expression of dapScer\UAS.cLa under the control of Scer\GAL4neur-P72.

Histoblast clones that express dapScer\UAS.cLa under the control of Scer\GAL4Act5C.PI do not proliferate. This results in a profound delay in larval epidermis cell death. Developmental timing is not affected in these animals but pharate adult esacpers show multiple differentiation and morphogenetic abdominal defects.

The distance between wing veins L3 and L4 at the wing margin is reduced in animals expressing dapScer\UAS.cLa under the control of Scer\GAL4ptc-559.1.

There is a decrease in S phase cells in cells expressing dapScer\UAS.cLa under the control of Scer\GAL4ptc-559.1 in the wing disc.

Expression of dapScer\UAS.cLa under the control of Scer\GAL4vg.int2.1 results in nicks in the wing.

Over-expression of dapScer\UAS.cLa under the control of Scer\GAL4GMR.PU blocks the second wave of mitosis posterior to the morphogenetic furrow in the eye imaginal disc.

Overexpression of dapScer\UAS.cLa under the control of Scer\GAL4neur-P72 triggers a G1-phase in pIIa and pIIb cells.

Overexpression of dapScer\UAS.cLa, under the regulation of Scer\GAL4sca-537.4 results in a reduction in the number of neurons in the NB6-4t lineage (from the wild-type number of 6 to 2-4 in approximately 81% of hemisegments), but homeotic transformation of the lineage does not occur. In addition, the number of glial cells is not affected, neither in the thorax nor in the abdomen.

When dapScer\UAS.cLa is driven by Scer\GAL4twi.PG, most hemisegments of mutant embryos show only one eve expressing pericardial cell (EPC), suggesting that the last division generating two EPCs did not occur. A reduction is also seen in the number of myocardial cells in these mutants. When dapScer\UAS.cLa is driven by Scer\GAL4eve.eme, both DA1 and DO2 founders form, but the number of EPCs is reduced to half.

Females expressing dapScer\UAS.cLa under the control of Scer\GAL4c323 are sterile, and lay flaccid eggs with thin eggshells and smaller dorsal appendages than normal.

Scer\GAL4F4-mediated expression results in inhibition of endoreplication.

Scer\GAL4prd.RG1 mediated overexpression results in a stable G1 arrest.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Scer\GAL4vg.int2.1, dapUAS.cLa has visible phenotype, enhanceable by vg[+]/vgnull

Suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Scer\GAL4vg.int2.1, dapUAS.cLa has wing phenotype, enhanceable by vg[+]/vgnull

Suppressed by
Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Overexpression of dapScer\UAS.cLa in the imaginal discs does not affect the amount of apoptosis seen in BubR1VDRC.cUa knockdown cells (with all transgenes under the control of Scer\GAL4en.PU or Scer\GAL4hh.PU).

Overexpression of dapScer\UAS.cLa in the imaginal discs greatly reduces the amount of apoptosis seen in bskKK108156 and BubR1VDRC.cUa knockdown cells (with all transgenes under the control of Scer\GAL4en.PU or Scer\GAL4hh.PU).

The severity of the wing nicking phenotype caused by expression of dapScer\UAS.cLa under the control of Scer\GAL4vg.int2.1 is greatly enhanced by vgnull/+.

Over-expression of Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG and dapScer\UAS.cLa under the control of Scer\GAL4GMR.PU does not alter the ability of Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG to induce ectopic cell division in the eye disc.

Expression of dapScer\UAS.cLa along the dorsal/ventral boundary of the wing under the control of Scer\GAL4vg.int2.1 in a vgnull/+ mutant background results in nicks at the wing tips. There is a significant decrease in mitotic cells along the dorsal/ventral boundary.

Co-expression of vgScer\UAS.cZa in flies expressing dapScer\UAS.cLa at the dorsal/ventral wing boundary under the control of Scer\GAL4vg.int2.1 in a vgnull/+ mutant background rescues wing morphology, and mitotic activity of cells is restored in this region.

Co-expression of dapScer\UAS.cLa blocks the ability of ciScer\UAS.cDa (expressed under the control of Scer\GAL4Act5C.PP) to induce S phase in clones in the morphogenetic furrow.

Expression of dapScer\UAS.cLa under the control of Scer\GAL4vg.PU in a vgnull/+ background results in a mutant wing phenotype.

Simultaneous expression of CycEScer\UAS.cLa completely rescues the cell cycle arrest induced by Scer\GAL4prd.RG1 mediated overexpression of dapScer\UAS.cLa.

Xenogenetic Interactions
Statement
Reference

Expression of dapScer\UAS.cLa greatly enhances the eye phenotypes seen when Mmus\Shisa5Scer\UAS.cGa is expressed under the control of Scer\GAL4ey.PH.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Incorporation of BrdU is undetectable in virtually all follicle cells in egg chambers of females expressing dapScer\UAS.cLa under the control of Scer\GAL4c323 or Scer\GAL4hs.PB.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
dapScer\UAS.cLa
dapUAS.cLa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Lane
Secondary FlyBase IDs
    References (26)