|Name||Saccharomyces cerevisiae UAS construct a of Shen||FlyBase ID||FBal0057664|
|Feature type||allele||Associated gene||Dmel\dac|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
(Chen et al., 1997, Jiao et al., 2001, Rauskolb, 2002.2.5, Rauskolb, 2001, Anderson et al., 2006, Wu and Cohen, 2000, Noveen et al., 2000, Curtiss and Mlodzik, 2000, Bui et al., 2000, Bui et al., 2000, Chen et al., 1999, Czerny et al., 1999, Miguel-Aliaga et al., 2004, Dong et al., 2001, Shen and Mardon, 1997, Baumgardt et al., 2007, Salzer et al., 2010, Emmons et al., 2007, Giorgianni and Mann, 2011)
|Phenotype Manifest In|
Expression of dacScer\UAS.cSa, under the control of Scer\GAL4GMR.long, results in the formation of a small ectopic eye along the anterior margin of the head cuticle. Expression of dacScer\UAS.cSa, under the control of Scer\GAL47B, results in ectopic eye development within the female genitals, but not within male genitals.
Expression of dacScer\UAS.cSa under the control of Scer\GAL4dpp.blk1 results in the differentiation of medial leg structures (such as bracted bristles) in 100% of antennae.
Expression of dacScer\UAS.cSa under the control of Scer\GAL4ey.PH results pharate adults with a headless phenotype. Four classes of phenotype are seen; class I pharate adults lack all head structures derived from the eye-antennal discs, class II consists of eyeless flies which lack most head structures and both antennae, class III consists of eyeless animals with large parts of the head missing but one or both antennae present and class IV consists of animals in which most of the head and one or both eyes of reduced size are present.
Expression of dacScer\UAS.cSa under the control of Scer\GAL4ptc-559.1 results in fusion of segments in the leg.
Expression of dacScer\UAS.cSa driven by Scer\GAL4dpp.blk1 causes ectopic retinal development, leading to the eventual formation of ectopic compound eyes in the adult head.
Expression of dacScer\UAS.cSa under the control of Scer\GAL4ey.PH results in flies with smaller eyes than normal (which contain fewer facets than wild-type eyes). The regular arrangement of facets is lost in these eyes. Expression of dacScer\UAS.cSa under the control of Scer\GAL4dpp.blk1 results in the induction of ectopic eyes. Expression of dacScer\UAS.cSa under the control of Scer\GAL4ey.PH results in a variable decrease in the size of dppd-blk eyes.
When expression is driven by Scer\GAL4238Y minor defects in the mushroom body are evident.
Expression of dacScer\UAS.cSa under the control of Scer\GAL430A does not result in the induction of ectopic retinal cells in the wing disc.
Ectopic expression of dacScer\UAS.cSa using Scer\GAL4dpp.blk1 produces very small ectopic eyes on the anterior surface of the head ventral to the antennae in 56% of flies. Leg and wing morphology are severely disrupted.
In a wild type background Scer\GAL4dpp.blk1 mediated expression truncates the adult eye in the dorsal-ventral dimension but ommatidial assembly is normal. Expression in the antennal disc and at the anterior-posterior compartment boundary of the leg and wing discs causes increased cell death and truncation of the antenna, leg and wing respectively. 20% of these flies develop ectopic eyes just ventral to the antenna on the anterior surface of the head. Eyes comprise up to 40 to 50 ommatidia that are of abnormal shape (rhabdomeres are unusually small and disorganised) and have irregular bristle arrangement. Ectopic expression also induces retinal development on the thorax just dorsal to the prothoracic leg, only 5% present obvious ommatidial structures. Patches of red pigment are also seen on the leg and wing with no well formed ommatidial structures.
Scer\GAL4dpp.blk1, dacScer\UAS.cSa has visible phenotype, enhanceable by eyaE7.Scer\UAS/Scer\GAL4dpp.blk1
Scer\GAL4dpp.blk1, dacScer\UAS.cSa has visible phenotype, enhanceable by eyaE11.Scer\UAS/Scer\GAL4dpp.blk1
Scer\GAL4dpp.blk1, dacScer\UAS.cSa has visible phenotype, enhanceable by Scer\GAL4dpp.blk1/eyaScer\UAS.cPa
|Phenotype Manifest In|
eyaE7.Scer\UAS shows a synergy in ectopic eye formation when coexpressed under the control of Scer\GAL4dpp.blk1 with dacScer\UAS.cSa. eyaE11.Scer\UAS shows a synergy in ectopic eye formation when coexpressed under the control of Scer\GAL4dpp.blk1 with dacScer\UAS.cSa.
Enhances the ectopic retinal development phenotype in the wing and haltere discs caused by expression of eyScer\UAS.cHa under the control of Scer\GAL430A. When eyScer\UAS.cHa and dacScer\UAS.cSa are coexpressed under the control of Scer\GAL430A, large ectopic eyes are seen on the wing and haltere hinges with complete penetrance. Clear ommatidial structures are seen on both the dorsal and ventral side of the wing hinge and the structure of the lens and interommatidial bristles is similar to wild-type.
Coexpression of eyaScer\UAS.cPa and dacScer\UAS.cSa using Scer\GAL4dpp.blk1 induces substantial ectopic eyes on the head, legs, wings and dorsal thorax. The cuticle between the normal eye field and the antenna is transformed into retinal cells such that the normal retinal field is expanded. Large patches of pigment are induced on the dorsal side of the femur and tibia of all legs, which are severely truncated. Ommatidial structures are observed in these patches. Red pigment, but no clear ommatidial morphology, is induced on the wing blade. Ectopic eyes are induced bilaterally on the dorsal thorax. All these phenotypes have 100% penetrance. Coexpression of eyaScer\UAS.cPa and dacScer\UAS.cSa using Scer\GAL4dpp.blk1 induces ectopic morphogenetic furrow advancement from the ventral side of the eye in late larval eye-antennal discs. Substantial ectopic photoreceptor development is also seen. The axons of these ectopic photoreceptors form a bundle that extends posteriorly into the eye imaginal disc. These axons appear to fuse with the axon tracts sent out by photoreceptors of the normal retinal field and probably exit through the optic stalk and synapse with the larval brain. Ectopic neurons are induced in leg and wing discs. Axons extended by these neurons retract during late larval and pupal development.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of Shen
|Secondary FlyBase IDs|
|References ( 19 )|
|Personal communication to FlyBase|