Allele Dmel\dac^Scer\UAS.cSa

General Information
Symbol	Dmel\dac^Scer\UAS.cSa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Shen	FlyBase ID	FBal0057664
Feature type	allele	Associated gene	Dmel\dac

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Shen and Mardon, 1997, Chen et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Full length dac cDNA is cloned into the pUAST vector. (Shen and Mardon, 1997)
Carried in construct	P{UAS-dac.S} (Chen et al., 1997, Jiao et al., 2001, Rauskolb, 2002.2.5, Rauskolb, 2001, Anderson et al., 2006, Wu and Cohen, 2000, Noveen et al., 2000, Curtiss and Mlodzik, 2000, Bui et al., 2000, Bui et al., 2000, Chen et al., 1999, Czerny et al., 1999, Miguel-Aliaga et al., 2004, Dong et al., 2001, Shen and Mardon, 1997, Baumgardt et al., 2007, Salzer et al., 2010, Emmons et al., 2007, Giorgianni and Mann, 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^hs.PB (Shen and Mardon, 1997) visible, with Scer\GAL4^7B (Anderson et al., 2006) visible, with Scer\GAL4^dpp.blk1 (Chen et al., 1997, Curtiss and Mlodzik, 2000, Dong et al., 2001) visible, with Scer\GAL4^ey.PH (Jiao et al., 2001, Curtiss and Mlodzik, 2000) visible, with Scer\GAL4^GMR.long (Anderson et al., 2006) visible, with Scer\GAL4^ptc-559.1 (Rauskolb, 2001)
Phenotype Manifest In
	adult head, with Scer\GAL4^dpp.blk1 (Chen et al., 1997) adult head, with Scer\GAL4^ey.PH (Jiao et al., 2001) antenna, with Scer\GAL4^dpp.blk1 (Dong et al., 2001, Shen and Mardon, 1997) antenna, with Scer\GAL4^ey.PH (Jiao et al., 2001) bract \| ectopic, with Scer\GAL4^dpp.blk1 (Dong et al., 2001) eye, with Scer\GAL4^dpp.blk1 (Shen and Mardon, 1997) eye, with Scer\GAL4^ey.PH (Jiao et al., 2001, Curtiss and Mlodzik, 2000) eye \| ectopic, with Scer\GAL4^dpp.blk1 (Chen et al., 1997, Curtiss and Mlodzik, 2000, Bui et al., 2000, Shen and Mardon, 1997) eye \| ectopic & adult head, with Scer\GAL4^GMR.long (Anderson et al., 2006) eye \| ectopic & female genitalia, with Scer\GAL4^7B (Anderson et al., 2006) interommatidial bristle, with Scer\GAL4^dpp.blk1 (Shen and Mardon, 1997) joint, with Scer\GAL4^ptc-559.1 (Rauskolb, 2001) leg, with Scer\GAL4^dpp.blk1 (Chen et al., 1997, Shen and Mardon, 1997) leg, with Scer\GAL4^ptc-559.1 (Rauskolb, 2001) mushroom body, with Scer\GAL4^238Y (Noveen et al., 2000) ommatidium, with Scer\GAL4^ey.PH (Curtiss and Mlodzik, 2000) rhabdomere, with Scer\GAL4^dpp.blk1 (Shen and Mardon, 1997) ventral thoracic disc \| anterior/posterior compartment boundary, with Scer\GAL4^dpp.blk1 (Shen and Mardon, 1997) wing, with Scer\GAL4^dpp.blk1 (Chen et al., 1997, Shen and Mardon, 1997) wing disc \| anterior/posterior compartment boundary, with Scer\GAL4^dpp.blk1 (Shen and Mardon, 1997)
Detailed Description
	Statement Reference Expression of dac^Scer\UAS.cSa, under the control of Scer\GAL4^GMR.long, results in the formation of a small ectopic eye along the anterior margin of the head cuticle. Expression of dac^Scer\UAS.cSa, under the control of Scer\GAL4^7B, results in ectopic eye development within the female genitals, but not within male genitals. (Anderson et al., 2006) Expression of dac^Scer\UAS.cSa under the control of Scer\GAL4^dpp.blk1 results in the differentiation of medial leg structures (such as bracted bristles) in 100% of antennae. (Dong et al., 2001) Expression of dac^Scer\UAS.cSa under the control of Scer\GAL4^ey.PH results pharate adults with a headless phenotype. Four classes of phenotype are seen; class I pharate adults lack all head structures derived from the eye-antennal discs, class II consists of eyeless flies which lack most head structures and both antennae, class III consists of eyeless animals with large parts of the head missing but one or both antennae present and class IV consists of animals in which most of the head and one or both eyes of reduced size are present. (Jiao et al., 2001) Expression of dac^Scer\UAS.cSa under the control of Scer\GAL4^ptc-559.1 results in fusion of segments in the leg. (Rauskolb, 2001) Expression of dac^Scer\UAS.cSa driven by Scer\GAL4^dpp.blk1 causes ectopic retinal development, leading to the eventual formation of ectopic compound eyes in the adult head. (Bui et al., 2000) Expression of dac^Scer\UAS.cSa under the control of Scer\GAL4^ey.PH results in flies with smaller eyes than normal (which contain fewer facets than wild-type eyes). The regular arrangement of facets is lost in these eyes. Expression of dac^Scer\UAS.cSa under the control of Scer\GAL4^dpp.blk1 results in the induction of ectopic eyes. Expression of dac^Scer\UAS.cSa under the control of Scer\GAL4^ey.PH results in a variable decrease in the size of dpp^d-blk eyes. (Curtiss and Mlodzik, 2000) When expression is driven by Scer\GAL4^238Y minor defects in the mushroom body are evident. (Noveen et al., 2000) Expression of dac^Scer\UAS.cSa under the control of Scer\GAL4^30A does not result in the induction of ectopic retinal cells in the wing disc. (Chen et al., 1999) Ectopic expression of dac^Scer\UAS.cSa using Scer\GAL4^dpp.blk1 produces very small ectopic eyes on the anterior surface of the head ventral to the antennae in 56% of flies. Leg and wing morphology are severely disrupted. (Chen et al., 1997) In a wild type background Scer\GAL4^dpp.blk1 mediated expression truncates the adult eye in the dorsal-ventral dimension but ommatidial assembly is normal. Expression in the antennal disc and at the anterior-posterior compartment boundary of the leg and wing discs causes increased cell death and truncation of the antenna, leg and wing respectively. 20% of these flies develop ectopic eyes just ventral to the antenna on the anterior surface of the head. Eyes comprise up to 40 to 50 ommatidia that are of abnormal shape (rhabdomeres are unusually small and disorganised) and have irregular bristle arrangement. Ectopic expression also induces retinal development on the thorax just dorsal to the prothoracic leg, only 5% present obvious ommatidial structures. Patches of red pigment are also seen on the leg and wing with no well formed ommatidial structures. (Shen and Mardon, 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^dpp.blk1, dac^Scer\UAS.cSa has visible phenotype, enhanceable by eya^E7.Scer\UAS/Scer\GAL4^dpp.blk1 (Bui et al., 2000) Scer\GAL4^dpp.blk1, dac^Scer\UAS.cSa has visible phenotype, enhanceable by eya^E11.Scer\UAS/Scer\GAL4^dpp.blk1 (Bui et al., 2000) Scer\GAL4^dpp.blk1, dac^Scer\UAS.cSa has visible phenotype, enhanceable by Scer\GAL4^dpp.blk1/eya^Scer\UAS.cPa (Chen et al., 1997) Scer\GAL4^ey.PH, dac^Scer\UAS.cSa has visible phenotype, enhanceable by dm^Scer\UAS.cZa/Scer\GAL4^ey.PH (Jiao et al., 2001)
Suppressed by
Statement Reference Scer\GAL4^ey.PH, dac^Scer\UAS.cSa has visible phenotype, suppressible \| partially by CycE^Scer\UAS.cLa, Scer\GAL4^ey.PH (Jiao et al., 2001)
Enhancer of
Statement Reference Scer\GAL4^30A/dac^Scer\UAS.cSa is an enhancer of visible phenotype of Scer\GAL4^30A, ey^Scer\UAS.cHa (Chen et al., 1999) Scer\GAL4^ey.PH/dac^Scer\UAS.cSa is an enhancer of visible \| recessive phenotype of dpp^d-blk (Curtiss and Mlodzik, 2000)
Other
Statement Reference Scer\GAL4^dpp.blk1, dac^Scer\UAS.cSa, eya^Scer\UAS.cPa has visible phenotype (Chen et al., 1997)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^ey.PH, dac^Scer\UAS.cSa has adult head phenotype, enhanceable by dm^Scer\UAS.cZa/Scer\GAL4^ey.PH (Jiao et al., 2001)
Suppressed by
Statement Reference Scer\GAL4^ey.PH, dac^Scer\UAS.cSa has adult head phenotype, suppressible \| partially by CycE^Scer\UAS.cLa, Scer\GAL4^ey.PH (Jiao et al., 2001)
Enhancer of
Statement Reference Scer\GAL4^30A/dac^Scer\UAS.cSa is an enhancer of haltere phenotype of Scer\GAL4^30A, ey^Scer\UAS.cHa (Chen et al., 1999) Scer\GAL4^30A/dac^Scer\UAS.cSa is an enhancer of wing disc phenotype of Scer\GAL4^30A, ey^Scer\UAS.cHa (Chen et al., 1999) Scer\GAL4^30A/dac^Scer\UAS.cSa is an enhancer of wing phenotype of Scer\GAL4^30A, ey^Scer\UAS.cHa (Chen et al., 1999) Scer\GAL4^ey.PH/dac^Scer\UAS.cSa is an enhancer of eye phenotype of dpp^d-blk (Curtiss and Mlodzik, 2000)
Other
Statement Reference Scer\GAL4^dpp.blk1, dac^Scer\UAS.cSa, eya^E7.Scer\UAS has eye \| ectopic phenotype (Bui et al., 2000, Bui et al., 2000) Scer\GAL4^dpp.blk1, dac^Scer\UAS.cSa, eya^E11.Scer\UAS has eye \| ectopic phenotype (Bui et al., 2000, Bui et al., 2000) Scer\GAL4^dpp.blk1, dac^Scer\UAS.cSa, eya^Scer\UAS.cPa has adult head phenotype (Chen et al., 1997, Chen et al., 1997) Scer\GAL4^dpp.blk1, dac^Scer\UAS.cSa, eya^Scer\UAS.cPa has dorsal mesothorax phenotype (Chen et al., 1997, Chen et al., 1997) Scer\GAL4^dpp.blk1, dac^Scer\UAS.cSa, eya^Scer\UAS.cPa has eye-antennal disc phenotype (Chen et al., 1997, Chen et al., 1997) Scer\GAL4^dpp.blk1, dac^Scer\UAS.cSa, eya^Scer\UAS.cPa has femur \| dorsal phenotype (Chen et al., 1997, Chen et al., 1997) Scer\GAL4^dpp.blk1, dac^Scer\UAS.cSa, eya^Scer\UAS.cPa has tibia \| dorsal phenotype (Chen et al., 1997, Chen et al., 1997) Scer\GAL4^dpp.blk1, dac^Scer\UAS.cSa, eya^Scer\UAS.cPa has ventral thoracic disc phenotype (Chen et al., 1997, Chen et al., 1997) Scer\GAL4^dpp.blk1, dac^Scer\UAS.cSa, eya^Scer\UAS.cPa has wing disc phenotype (Chen et al., 1997, Chen et al., 1997) Scer\GAL4^dpp.blk1, dac^Scer\UAS.cSa, eya^Scer\UAS.cPa has wing phenotype (Chen et al., 1997, Chen et al., 1997)
Additional Comments
Genetic Interactions
Statement Reference eya^E7.Scer\UAS shows a synergy in ectopic eye formation when coexpressed under the control of Scer\GAL4^dpp.blk1 with dac^Scer\UAS.cSa. eya^E11.Scer\UAS shows a synergy in ectopic eye formation when coexpressed under the control of Scer\GAL4^dpp.blk1 with dac^Scer\UAS.cSa. (Bui et al., 2000) Enhances the ectopic retinal development phenotype in the wing and haltere discs caused by expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^30A. When ey^Scer\UAS.cHa and dac^Scer\UAS.cSa are coexpressed under the control of Scer\GAL4^30A, large ectopic eyes are seen on the wing and haltere hinges with complete penetrance. Clear ommatidial structures are seen on both the dorsal and ventral side of the wing hinge and the structure of the lens and interommatidial bristles is similar to wild-type. (Chen et al., 1999) Coexpression of eya^Scer\UAS.cPa and dac^Scer\UAS.cSa using Scer\GAL4^dpp.blk1 induces substantial ectopic eyes on the head, legs, wings and dorsal thorax. The cuticle between the normal eye field and the antenna is transformed into retinal cells such that the normal retinal field is expanded. Large patches of pigment are induced on the dorsal side of the femur and tibia of all legs, which are severely truncated. Ommatidial structures are observed in these patches. Red pigment, but no clear ommatidial morphology, is induced on the wing blade. Ectopic eyes are induced bilaterally on the dorsal thorax. All these phenotypes have 100% penetrance. Coexpression of eya^Scer\UAS.cPa and dac^Scer\UAS.cSa using Scer\GAL4^dpp.blk1 induces ectopic morphogenetic furrow advancement from the ventral side of the eye in late larval eye-antennal discs. Substantial ectopic photoreceptor development is also seen. The axons of these ectopic photoreceptors form a bundle that extends posteriorly into the eye imaginal disc. These axons appear to fuse with the axon tracts sent out by photoreceptors of the normal retinal field and probably exit through the optic stalk and synapse with the larval brain. Ectopic neurons are induced in leg and wing discs. Axons extended by these neurons retract during late larval and pupal development. (Chen et al., 1997)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^dpp.blk1/dac^Scer\UAS.cSa rescues dac¹ (Shen and Mardon, 1997) Scer\GAL4^dpp.blk1/dac^Scer\UAS.cSa rescues dac³ (Shen and Mardon, 1997)
Comments	Scer\GAL4^dpp.blk1 mediated expression of dac^Scer\UAS.cSa rescues eye development in dac³ and dac¹ homozygotes (it fully rescues the morphogenetic furrow initiation defect) but the ommatidial array is disorganised. (Shen and Mardon, 1997)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	dac^Scer\UAS.cSa dac^UAS.cSa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Shen (Shen and Mardon, 1997)
Secondary FlyBase IDs

References ( 19 )
Research paper	Giorgianni and Mann, 2011, Dev. Cell 20(4): 455--468 Establishment of Medial Fates along the Proximodistal Axis of the Drosophila Leg through Direct Activation of dachshund by Distalless. [FBrf0213490] Salzer et al., 2010, Genetics 184(1): 185--197 The retinal determination gene eyes absent is regulated by the EGF receptor pathway throughout development in Drosophila. [FBrf0209690] Baumgardt et al., 2007, PLoS Biol. 5(2): e37 Specification of neuronal identities by feedforward combinatorial coding. [FBrf0195184] Emmons et al., 2007, Dev. Biol. 302(2): 412--426 Regulation of the Drosophila distal antennal determinant spineless. [FBrf0193220] Anderson et al., 2006, Dev. Biol. 297(2): 536--549 Regulation of the retinal determination gene dachshund in the embryonic head and developing eye of Drosophila. [FBrf0193889] Miguel-Aliaga et al., 2004, Development 131(23): 5837--5848 Independent roles of the dachshund and eyes absent genes in BMP signaling, axon pathfinding and neuronal specification. [FBrf0180178] Dong et al., 2001, Development 128(12): 2365--2372 Proximodistal domain specification and interactions in developing Drosophila appendages. [FBrf0137225] Jiao et al., 2001, Development 128(17): 3307--3319 Headless flies generated by developmental pathway interference. [FBrf0138373] Rauskolb, 2001, Development 128(22): 4511--4521 The establishment of segmentation in the Drosophila leg. [FBrf0141484] Bui et al., 2000, Genetics 155(2): 709--720 Molecular analysis of Drosophila eyes absent mutants reveals features of the conserved eya domain. [FBrf0128419] Bui et al., 2000, Dev. Biol. 221(2): 355--364 Functional analysis of an eye enhancer of the Drosophila eyes absent gene: differential regulation by eye specification genes. [FBrf0127029] Curtiss and Mlodzik, 2000, Development 127(6): 1325--1336 Morphogenetic furrow initiation and progression during eye development in Drosophila: the roles of decapentaplegic, hedgehog and eyes absent. [FBrf0125176] Noveen et al., 2000, Development 127(16): 3475--3488 Early development of the Drosophila mushroom body: the roles of eyeless and dachshund. [FBrf0128595] Wu and Cohen, 2000, Mech. Dev. 94(1,2): 47--56 Proximal distal axis formation in the Drosophila leg: distinct functions of teashirt and homothorax in the proximal leg. [FBrf0128683] Chen et al., 1999, Development 126(5): 935--943 Signaling by the TGF-beta homolog decapentaplegic functions reiteratively within the network of genes controlling retinal cell fate determination in Drosophila. [FBrf0106322] Czerny et al., 1999, Mol. Cell 3(3): 297--307 twin of eyeless, a second Pax-6 gene of Drosophila, acts upstream of eyeless in the control of eye development. [FBrf0107668] Chen et al., 1997, Cell 91(7): 893--903 Dachshund and eyes absent proteins form a complex and function synergistically to induce ectopic eye development in Drosophila. [FBrf0099986] Shen and Mardon, 1997, Development 124(1): 45--52 Ectopic eye development in Drosophila induced by directed dachshund expression. [FBrf0091167]
Personal communication to FlyBase	Rauskolb, 2002.2.5, UASDll and UASdac21M5. UASDll and UASdac21M5. [FBrf0145597]

Allele Dmel\dacScer\UAS.cSa

Allele Dmel\dac^Scer\UAS.cSa